5 replies to this topic

[thedevinroy]

Increasing NAD/NADH ratio has long been a topic of discussion here. Some people not taking NAD lozenges have notably improved their cognition. Many people who take NADH report to having no effects. NAD and NADH are created by the body using smaller molecules such as niacin. Studies show that NR (nicotinamide riboside) is great for elevating NAD levels.

http://www.ncbi.nlm....pubmed/15137942

NAD+ is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADPribosyl groups used in various reactions, including those that retard aging in experimental systems. Nicotinic acid and nicotinamide were defined as the vitamin precursors of NAD+ in Elvehjem's classic discoveries of the 1930s. The accepted view of eukaryotic NAD+ biosynthesis, that all anabolism flows through nicotinic acid mononucleotide, was challenged experimentally and revealed that nicotinamide riboside is an unanticipated NAD+ precursor in yeast. Nicotinamide riboside kinases from yeast and humans essential for this pathway were identified and found to be highly specific for phosphorylation of nicotinamide riboside and the cancer drug tiazofurin. Nicotinamide riboside was discovered as a nutrient in milk, suggesting that nicotinamide riboside is a useful compound for elevation of NAD+ levels in humans.

Life Extension Vitamins also did an article here: http://www.lifeexten.../05gotniri.html

This compound is found in milk! Cow's milk or people milk... not sure.

The reason NR works so great is that it is a more complete precursor. A quick glance at the molecular structure of each will reveal that niacin is a very small piece of the puzzle in comparison. Adding one quinone (from tryptophan) and two orthophosphates (from ATP) produces NAD. Therefore, tryptophan should be taken in conjunction with NR.

Are there any other sources of NAD or NR? Are there more ways of increasing the NAD/NADH ratio? Has anyone received a bulk powder shipment of NAD or NR?

Edited by devinthayer, 22 June 2011 - 08:10 PM.

[thedevinroy]

Found the full study at http://www.biochem.u...ganowski04a.pdf

Also, they tell you how to make a milk extract...

Nicotinamide Riboside and Whey Preparations
NAD+ (Sigma) concentration was determined by conversion to NADH with alcohol dehydrogenase using an absorption coefficient (340 nm) of 6200 cm-1 M-1. The concentration of NMN was determined by converting NAD+ to NMN plus AMP with rattlesnake venom NAD+ pyrophosphatase (E.C. 3.6.9.1, Sigma). Using 15,400 cm-1 M-1 as the absorption coefficient for AMP at 259 nm, we used relative peak areas to calculate the absorption coefficient (259 nm) of NMN to be 4740 cm-1 M-1. To prepare nicotinamide riboside, 120 umol NMN (Sigma, concentration corrected by absorption) was treated with 1250 units of calf intestinal alkaline phosphatase (Sigma) for 1 hr at 37°C in 1 ml 100 mM NaCl, 20 mM Tris [pH 8.0], 5 mM MgCl2. After hydrolysis of NMN to nicotinamide riboside was verified by HPLC, phosphatase was removed by centrifuging the reaction through a 5000 Da filter (Millipore). A whey vitamin fraction of commercial nonfat cow's milk was prepared by adjusting the pH to 4 with HCl, stirring at 55°C for 10 min, removal of denatured casein by centrifugation, and passage through a 5000 Da filter. In yeast media, nicotinamide riboside was used at 10 uM and whey vitamin fraction at 50% by volume.

Anyone got some milk extract?

Edited by devinthayer, 22 June 2011 - 09:28 PM.

[aaron43]

Found the full study at http://www.biochem.u...ganowski04a.pdf

Also, they tell you how to make a milk extract...

Nicotinamide Riboside and Whey Preparations
NAD+ (Sigma) concentration was determined by conversion to NADH with alcohol dehydrogenase using an absorption coefficient (340 nm) of 6200 cm-1 M-1. The concentration of NMN was determined by converting NAD+ to NMN plus AMP with rattlesnake venom NAD+ pyrophosphatase (E.C. 3.6.9.1, Sigma). Using 15,400 cm-1 M-1 as the absorption coefficient for AMP at 259 nm, we used relative peak areas to calculate the absorption coefficient (259 nm) of NMN to be 4740 cm-1 M-1. To prepare nicotinamide riboside, 120 umol NMN (Sigma, concentration corrected by absorption) was treated with 1250 units of calf intestinal alkaline phosphatase (Sigma) for 1 hr at 37°C in 1 ml 100 mM NaCl, 20 mM Tris [pH 8.0], 5 mM MgCl2. After hydrolysis of NMN to nicotinamide riboside was verified by HPLC, phosphatase was removed by centrifuging the reaction through a 5000 Da filter (Millipore). A whey vitamin fraction of commercial nonfat cow's milk was prepared by adjusting the pH to 4 with HCl, stirring at 55°C for 10 min, removal of denatured casein by centrifugation, and passage through a 5000 Da filter. In yeast media, nicotinamide riboside was used at 10 uM and whey vitamin fraction at 50% by volume.

Anyone got some milk extract?

http://www.longecity...-nadnadh-ratio/

[thedevinroy]

http://www.longecity...-nadnadh-ratio/

Thanks! Found that one already. Glad you mentioned it because I forgot to. Some great of ways of increasing the ratio mentioned were Aspartic acid, L-Serine, and Methylene Blue. L-Serine also reduces methionine which is great way to combat oxidative stress in mitochondria. There might be other ways, too if anyone else has heard of them.

I know there are NAD+ lozenges, but I was wondering if there was an digestible version or perhaps a NR extract from yeast or milk.

[Logic]

Bumping this as no-one has talked about Nicotinamide Riboside from Whey recently.
This info may even bring the price of NR down to reasonable again!

L-Serine, and Methylene Blue are also interesting but questions abound as to whether the NAD+ is in the right place within the cell to be effective?
http://www.ncbi.nlm..../emss-52498.pdf

The graphic in the liked paper
http://www.biochem.u...ganowski04a.pdf
is also interesting in that it points to finding a human equivalent of PNC1 (pyrazinamidase/nicotinamidase 1) is what we should actually be looking at.
http://www.researchg...yces_cerevisiae

(Does anyone know what's happened to Devinthayer?)

Edited by Logic, 30 September 2014 - 01:32 PM.

[Flex]

I´m also curious about what happened to Devinthayer