29 replies to this topic

[Allen Walters]

I've been putting it on my head for several weeks now and not one hair more than i had before.

[Allen Walters]

I have offered a guy with ms some mixture for free if he will keep a log of how he feels everyday. If he wants to do it I will post it on here.

[niner]

I have offered a guy with ms some mixture for free if he will keep a log of how he feels everyday. If he wants to do it I will post it on here.

That's great. Lister's roomate has MS and was taking 1.5mg/day, as I recall. She reported that her balance improved. The key things to record are the person's weight, the dose they are taking, and maybe something about the extent of their disease. I'm not sure a daily log is needed, but some sort of periodic reporting would be good.

[Allen Walters]

I also gave some to my cousin who has had sever asthma sense she was a little.

[Allen Walters]

I put the c60/oo mix in some dmso and stirred it for an hour, then let it settle for a couple hours. Th mix separated but the dmso has a yellow color now. Something dissolved into it. I'm gonna let it go all night and see what it looks like tomorrow.

[bcelliott]

I put the c60/oo mix in some dmso and stirred it for an hour, then let it settle for a couple hours. Th mix separated but the dmso has a yellow color now. Something dissolved into it. I'm gonna let it go all night and see what it looks like tomorrow.

Try the same experiment with just the olive oil as a control. If the color isn't as yellow, you've got nano-clusters of C60 in your other sample.

[Chopperboy]

I have noticed being more focused and very vivid dreams on C60.
Apart from that has anyone noticed anything else?

I have noticed being more focused and very vivid dreams on C60.
Apart from that has anyone noticed anything else?

[tintinet]

I have noticed being more focused and very vivid dreams on C60.
Apart from that has anyone noticed anything else?

I have noticed being more focused and very vivid dreams on C60.
Apart from that has anyone noticed anything else?

Deja vu?

I've also notice more vivid dreams. Not much focus, though....

Edited by tintinet, 31 August 2012 - 01:59 PM.

[Allen Walters]

i put some c60 in D-LIMONENE and it turned pink, so I assume it's forming an some kind of adduct. Now I should be able to mix it with DMSO and see if it grows hair.

[Allen Walters]

after a night of just sitting the mixture was a light pink. I put it on the mixer and in an hour almost all the c60 is dissolved and it's a deep purple color. I'm surprised at how fast it dissolves in the D-LIMONENE.

[niner]

i put some c60 in D-LIMONENE and it turned pink, so I assume it's forming an some kind of adduct. Now I should be able to mix it with DMSO and see if it grows hair.

C60 is kind of a pinkish-purple when it's dissolved in a solvent like toluene. Limonene is a hydrocarbon of similar size and shape; I think you might be looking at a dilute solution of C60 rather than an adduct. It's not inconceivable that limonene might react with C60 to form an addition compound, but if it did, it would be more likely for the color to be a darker red. I think the odds are good that you have a solution.

[Turnbuckle]

after a night of just sitting the mixture was a light pink. I put it on the mixer and in an hour almost all the c60 is dissolved and it's a deep purple color. I'm surprised at how fast it dissolves in the D-LIMONENE.

Interesting. D-limonene is being used to dissolve things like Co-Q 10 for packaging in gel caps.

For example, here's a patent describing this.

Edited by Turnbuckle, 08 September 2012 - 10:04 PM.

[zorba990]

after a night of just sitting the mixture was a light pink. I put it on the mixer and in an hour almost all the c60 is dissolved and it's a deep purple color. I'm surprised at how fast it dissolves in the D-LIMONENE.

Great experiment!
It would be interesting to see if the limonene-c60 mixture could then be more easily redissolved in Olive Oil.

[Turnbuckle]

after a night of just sitting the mixture was a light pink. I put it on the mixer and in an hour almost all the c60 is dissolved and it's a deep purple color. I'm surprised at how fast it dissolves in the D-LIMONENE.

Great experiment!
It would be interesting to see if the limonene-c60 mixture could then be more easily redissolved in Olive Oil.

Or forget the olive oil. Fullerene/limonene could be sold in gel caps, in which case you'd see the C60 market explode.

[Metrodorus]

Except, once again, we don't know if the active molecule is fullerene itself, or an olive -oil -fullerene adduct.

Edited by Metrodorus, 09 September 2012 - 12:33 PM.

[Mind]

Except, once again, we don't know if the active molecule is fullerene itself, or an olive -oil -fullerene adduct.

Thank You Metrodus! Also, with such a small sample size in the original Baati experiment, the odds of some gross error are greater. Proceed with caution.

[Turnbuckle]

Except, once again, we don't know if the active molecule is fullerene itself, or an olive -oil -fullerene adduct.

Indeed we don't, but since there are so many other positive side effects of C60 in EVOO, expect the value of C60 in limonene to be quickly ascertained by intrepid users.

[niner]

Except, once again, we don't know if the active molecule is fullerene itself, or an olive -oil -fullerene adduct.

There are two things that we do know: 1) The actual SOLUBILITY of unmodified (pristine) C60 in olive oil is only 3-6 micrograms per ml, as measured by Braun et al. 2) C60 REACTS with olive oil over a period of days, eventually forming solutions of a C60-fatty acid adduct in concentrations of up to 900 micrograms per ml, or two orders of magnitude higher than the dissolved, unreacted C60. These reactions, including the reactions with pure fatty acids were characterized chromatographically by Pogorelij. See also a related paper by Franco Cataldo http://www.sciencedi...09308410000496.

These two things strongly suggest that the active species is the adduct, not pristine C60.

[maxwatt]

Except, once again, we don't know if the active molecule is fullerene itself, or an olive -oil -fullerene adduct.

...

These two things strongly suggest that the active species is the adduct, not pristine C60.

One more factoid for us to chew: I store my C60-olive oil in the freezer.
It froze with a yellowish color, except for a thin top layer that was an intense dark red color. So it should be possible to isolate and concentrate the C60 adduct, and maybe cap it without all that olive oil.

However, the OO alone mice did see a 30% increase lifespan,and we do not know if the olive oil is necessary for the adduct to have a live-extending effect.

More mice, please.

[niner]

One more factoid for us to chew: I store my C60-olive oil in the freezer.
It froze with a yellowish color, except for a thin top layer that was an intense dark red color. So it should be possible to isolate and concentrate the C60 adduct, and maybe cap it without all that olive oil.

Cool discovery! (pun not intended, but I left it there anyway...) This sounds a little bit like zone refining. The adducts (there are multiple possibilities) can also be purified chromatographically, but just throwing it in the freezer is a hell of a lot easier. It could certainly be capped. It's really starting to look like two or three ml of the olive oil solution is an adequate daily dose, so if someone wanted caps, it would be possible to just cap the olive oil. I would just put it on a salad with some balsamic vinegar. And more olive oil...

[Allen Walters]

The Limonene I used is food grade high purity from oranges, and is crystal clear. It dissolved all the c60 within a couple hours on the stirrer. I didn't measure anything but if I had to guess I'd say it could hold a lot more c60 than olive oil can.

Edited by Allen Walters, 09 September 2012 - 03:58 PM.

[Allen Walters]

I don't have a camera to do time lapse with. I did start another batch today and put 100mg of c60 in 100ml of d-limonene. It started turning purple within minutes and now it's been going for 12 hrs. There is still lots of undissolved c60. My guess that it would hold more I think was wrong, but it is so dark now that my ultrafire flash light is almost completely blocked by 2in of the mixture.

[Allen Walters]

Ok, I hope it's fine to post my findings here. I let my C60/Limonene mixture settle overnight and there is still a lot of undissolved C60. It did clear up though. Here is a picture I took. Sure looks like adduct to me.

Attached Files

•  20120913_0140442.jpg   57.05KB   3 downloads

Edited by Allen Walters, 13 September 2012 - 03:21 PM.

[Turnbuckle]

Inspired by Allen Walters I brewed up a batch of C60/C70 in d-limonene using 18 miniutes of ultrasonic treatment to achieve a concentration of just under 1 mg/ml. The color was very dark, almost the same as when mixed in EVOO. After filtering I mixed 1 tsp in 2 tbs EVOO, which produced an orange color. Taking this 5 mg dose I felt fine for several hours, but then, working out in the yard, I felt more quickly fatiuged than normal and went to bed early, sleeping for ten hours--much longer than usual for me. I feel fine today but with no interest in taking it again. I think this is more evidence that the adducts play a big part in how this stuff acts. Thus it would be valuable to test each component of EVOO individually with C60. Chances are only one of them is truly active.

Note that I've taken C60/C70/EVOO before and found it hard to distinguish from C60/EVOO, but I hadn't taken anything ultarsonically mixed before. Also, I mixed the d-limonene into EVOO because limonene alone is very harsh.

Edited by Turnbuckle, 14 September 2012 - 11:55 AM.

[niner]

I feel fine today but with no interest in taking it again. I think this is more evidence that the adducts play a big part in how this stuff acts. Thus it would be valuable to test each component of EVOO individually with C60. Chances are only one of them is truly active.

When C60 reacts with olive oil, it's in the triglyceride form, although I suppose it's possible that there are some free fatty acids floating around. Once it's in the body, I see no reason why it wouldn't be hydrolyzed by lipases just like any triglyceride, since the active site of lipases accepts the glycerol part of the triglyceride molecule, while the C60 should be bound to the fatty acid hydrocarbon chain, based on the known chemistry of C60. In this paper, Franco Cataldo has reacted C60 with the methyl esters of pure fatty acids. This would be a route to characterizing the structures of the addition products. You could vary the chain length and saturation to produce a lot of different adducts. An additional complication is the regioisomerism of the C60: There are a number of different structures that a bis-adduct could take on, ranging from both being on the same side of the C60 to being on opposite sides. Tris- and tetrakis- adducts, should they exist, would present even more regioisomers.

Once you had a large selection of adducts, you'd need a screening assay that could rank them in some way without spending years in a gigantic animal experiment. It might well be sufficient to use a well-characterized cell line, dosing with each unique adduct, and measuring the effect on mitochondrial ROS and oxidation products. Ultimately, you'd want to do animal experiments with several of them.

The FDA frowns on uncharacterized mixtures, but a specific adduct shouldn't prevent a problem, and I would think that a specific adduct would be patentable, as well, unless someone has already laid claim to all adducts (likely), and it could be made to stick (maybe..). Anyway, if I were a pharmaceutical company, this is the program I'd take a shot at.

Limonene is a cyclohexene with methyl and isopropenyl substituents. It's loaded with allylic carbons and is very prone to oxidation. It's thus a very likely candidate for adduct formation. The thing is, this is a very different adduct from the fatty acid adducts. Cataldo shows the absorption spectrum of cyclohexene-C60 in his paper, and it looks pretty different from the various fatty acids. It's likely to have a very different distribution in the body, and might find itself in compartments where it's doing more harm than good- For example, it might interfere with free radical-based signaling, or some such thing. I'd stay away from it.

[Turnbuckle]

I have noticed being more focused and very vivid dreams on C60.

I've been taking C60 in the morning and hadn't noticed anything like this. Yesterday I took it in the evening and had a whole night of first-person dreams about India. I've never been to India and have never had a night where my dreams all had the same theme.

I also took co-Q10 mixed in d-limonene yesterday evening, and this may have something to do with it.

Edited by Turnbuckle, 16 September 2012 - 12:27 PM.

[Hebbeh]

I have noticed being more focused and very vivid dreams on C60.

I've been taking C60 in the morning and hadn't noticed anything like this. Yesterday I took it in the evening and had a whole night of first-person dreams about India. I've never been to India and have never had a night where my dreams all had the same theme.

I also took co-Q10 mixed in d-limonene yesterday evening, and this may have something to do with it.

I experience more vivid dreams with C60. But not to the extent that I did with Epitalon...the dreams were extreme on it. Prior to Epitalon and C60, I would rarely recall dreaming and on the occasion that I would, the dreams would be brief and minor. Now dreams seem more like being "in" a movie. And I wake and wonder where that came from. Had never experienced this before. But Epitalon is much stronger in this regard.

[Chopperboy]

I have noticed being more focused and very vivid dreams on C60.

I've been taking C60 in the morning and hadn't noticed anything like this. Yesterday I took it in the evening and had a whole night of first-person dreams about India. I've never been to India and have never had a night where my dreams all had the same theme.

I also took co-Q10 mixed in d-limonene yesterday evening, and this may have something to do with it.

I experience more vivid dreams with C60. But not to the extent that I did with Epitalon...the dreams were extreme on it. Prior to Epitalon and C60, I would rarely recall dreaming and on the occasion that I would, the dreams would be brief and minor. Now dreams seem more like being "in" a movie. And I wake and wonder where that came from. Had never experienced this before. But Epitalon is much stronger in this regard.

Well put!
Dreams do feel like being in a full technicolor movie that can be remembered in detail afterwards.
I wonder if C60 has an impact on DMT production in the pineal gland or assists transport/ uptake.

[elixir]

Hi
I just joined Longecity since I wanted to share my experience with c60 and vivid dreaming. I've had a distinct increase in what I remember from my dreaming, in drama, colour, etc. I'm fairly certain it has to do with my newly started c60-supplementation.

Some background (I haven't introduced myself). I'm in my early forties and since I began noticing signs of aging I have read a lot about lifestyle and supplementation. I have used Resveratrol, Vitamin D, Omega 3:s and Phosphatidylcholine for over a year. Sporadically I use piracetam and L-theanine.

I've been curious about the buckyballs ever since the study and last week I ordered from owndoc.com. Day one I took about 1 mg and the same night I hade a very clear and somewhat disturbing dream. I did not associate my dream to C-60. Three days later I took another 1,5 mg and had more dreams. The vivid dreaming continued day four and five when I took another 1.5 mg and the dreams continued and I started to suspect that something induced them. I'm now going to stay off C-60 for at least a week and see what happens.

I'm fairly sure that my "explosive" dreaming does not have a psychological explanation. The only lifestyle change I made was the c60 and for me, intake of a substance is not a big thing (Otherwise I would have waited 20 years for definitive answers on C60 cancer frequency and so on.)

So, what is the mechanism? I guess it proves that C60 is taken up in the bloodstream and works in some way, but I guess we are not talking about mitochondria here, but rather serotoninproduction or something. Apart from the dreams I have noticed that I feel slightly more energetic in an "artificial" way. I feels like some neurtransmittor is pushing harder. This could be a placebo effect though.

BTW I cannot find information on WHY drugs lika champix or some malaria medication affect dreaming. Does anybody know?

[tintinet]

I have noticed being more focused and very vivid dreams on C60.

I've been taking C60 in the morning and hadn't noticed anything like this. Yesterday I took it in the evening and had a whole night of first-person dreams about India. I've never been to India and have never had a night where my dreams all had the same theme.

I also took co-Q10 mixed in d-limonene yesterday evening, and this may have something to do with it.

I experience more vivid dreams with C60. But not to the extent that I did with Epitalon...the dreams were extreme on it. Prior to Epitalon and C60, I would rarely recall dreaming and on the occasion that I would, the dreams would be brief and minor. Now dreams seem more like being "in" a movie. And I wake and wonder where that came from. Had never experienced this before. But Epitalon is much stronger in this regard.

1+ for Epitalon dreams! Intense, vivid, but not necessarily always pleasant. I feel like falling asleep is almost like preparing for an expedition, lately.