It has been awhile since my last post on my personal NMN experience. I have been quietly continuing my experimenting with NMN dosing and application methods. I am sharing this information on an informal basis and it is not to be taken as any kind of medical recommendation or advice.
Below is my updated NMN log.
Start Date End Date Duration Product MG Dose Daily Freq. Daily dose Admin. Result
July 11, 2015 May 5, 2016 10 months NMN w/Act. 3,400 2 6,800 Liquid Good
May 6, 2016 May 21, 2017 12.5 months NONE Arthritis
May 22, 2017 Aug. 28, 2017 3 months Niagen NR 500 2 1,000 Capsule Arthritis
Aug. 29, 2017 Sept. 21, 2017 1 month NONE Arthritis
Sept. 22, 2017 Dec. 21, 2017 3 months NMN w/Act. 1,600 2 3,200 Liquid Good
Dec. 22, 2017 Apr. 26, 2018 4 months NMN w/Act. 750 2 1,500 Liquid Good
Apr. 27, 2018 May 8, 2018 2 weeks NMN w/Act. 500 2 1,000 Sublingual Good
May 9, 2018 June 3,2018 1 month NMN w/Act. 500 1 500 Sublingual Arthritis
June 4, 2018 June 24, 2018 3 weeks NMN w/Act. 500 2 1,000 Oral tablet Good
June 25, 2018 June 30, 2018 5 days NMN w/Act. 25 8 200 Sublingual Arthritis
July 1, 2018 July 18, 2018 2.5 weeks NMN w/Act. 500 2 1,000 Oral tablet Good
July 19, 2018 Sept. 24, 2018 2 months NMN w/Act. 50 7 350 Sublingual Good
Sept. 25, 2018 Oct.14, 2018 3 weeks NAD+ w/Act. 50 8 400 Sublingual Good
Oct. 15, 2018 Nov. 15, 2018 1 month NMN w/Act. 30 8 240 Sublingual Good
Nov. 16, 2018 Dec. 2, 2018 3 weeks NMN only 30 8 240 Sublingual Good
Dec. 3, 2018 NMN w/Act. 400 2 800 Oral tablet
Dec. 3, 2018 NMN w/Act. 30 6 180 Sublingual
So that more recent viewers will not have to reread this entire thread, I will recap. Early in 2014 a group of 7 males, aged 45 to 66, decided that we would do a group buy of NMN. Our designated lead guinea pig is a molecular biologist and he began taking NMN late in 2014.
In the beginning we monitored his blood work with monthly full panel blood tests performed by LabCorp. We believed that inflammation levels were a good indicator of health and aging, so we added two inflammation tests to the standard panel. The inflammation markers that we tracked were; C-Reactive Protein, (CRP), Tumor Necrosis Factor-Alpha (TNF-α) and Interleukin-6 (IL-6). As he began the NMN dosing (3,500 mgs of NMN twice per day) we noted a steady increase of inflammation peaking at week 4, followed by a significant decline to the lowest inflammation levels at month 2 and then a steady rise that stabilized by month 6 at a slight improvement to his baseline inflammation numbers.
NMN study participant #1, age 61
Baseline Month 1 Month 2 Month 3 Month 6
12/7/2014
C-Reactive Protein mg/L 2.77 3.25 0.43 0.53 0.85
TNF-α pg/L 1.10 0.90 1.10 1.10 1.00
IL-6 pg/L 1.30 4.40 <0.7 0.90 3.10
Total Inflammation Score 5.17 8.55 1.53 2.53 4.95
We theorized as follows: During the 1st month the NMN had activated all of the Sirtuins and they had gotten busy with all of their long neglected biological “housekeeping” functions. The inflammation levels rose to get this accomplished. Once the Sirtuins had gotten everything in order, the inflammation levels dropped to their low point at the end of month 2. We attributed the slow and steady rise back to the baseline inflammation levels at month 6 to a homeostatic negative biological feedback loop kicking in.
While his inflammation level was rising, we researched the issue and came up with a theory as to why that was happening. Our theory was that the increase of activity by Sirtuins was out of balance with the other anti-aging pathways and the body was reacting by shutting down the Sirtuin activity brought on by the increased level of NMN. We theorized that by activating 2 other major anti-aging pathways (mTOR and AMPK) that we may activate all 3 of these anti-aging pathways in a balanced manner thereby achieving a sustained homeostasis. We added 2 compounds to our NMN and awaited the results.
NMN study participant #1, age 61
Baseline Month 1 Month 2 Month 3 Month 6 Month 7 Month 8
12/7/2014
C-Reactive Protein mg/L 2.77 3.25 0.43 0.53 0.85 0.21 0.40
TNF-α pg/L 1.10 0.90 1.10 1.10 1.00 0.50 0.30
IL-6 pg/L 1.30 4.40 <0.7 0.90 3.10 <0.7 <0.7
Total Inflammation Score 5.17 8.55 1.53 2.53 4.95 1.40 1.40
We were very pleased with results and the other 6 members began taking NMN along with the 2 other compounds. All of our blood tests have showed the same dramatic and sustained decrease of inflammation. Since that time we have continued to research and experiment by adding and/or replacing compounds and adjusting their ratios to each other. In our current iteration we are using a 5 compound formulation to activate a total of 9 anti-aging pathways that sustains the NMN activation at its peak anti-inflammation levels.
On July 29, 2015 Yasumichi Arai published Inflammation, But Not Telomere Length, Predicts Successful Ageing at Extreme Old Age: A Longitudinal Study of Semi-supercentenarians in which the author wrote, “We conclude that inflammation is an important malleable driver of ageing up to extreme old age in humans.” Furthermore, “Together, our results suggest suppression of chronic inflammation as a major determinant of successful longevity, which is relevant over a very wide age range up to extreme old age.” And, “Our results suggest that the development of more sophisticated and safer anti-inflammatory strategies could be an essential step towards the prevention of human premature ageing.” And finally, “Despite these limitations, our study showed that over a very wide age range from 45 to 115 years, including unprecedentedly large numbers of the extremely old, inflammation is an important driver of ageing that might be amenable to future pharmacological intervention. Accordingly, designing novel, safe anti-inflammatory or immune-modulating medication has major potential to improve healthy lifespan.”
We believe that the answer to Dr. Arai’s quest is NMN.
Reviewing my various dosing regimens since my last post.
July 1, 2018 July 18, 2018 2.5 weeks NMN w/Act. 500 2 1,000 Oral tablet Good
500 mg twice per day of oral tablet NMN with activator worked just fine.
July 19, 2018 Sept. 24, 2018 2 months NMN w/Act. 50 7 350 Sublingual Good
Put together some 50 mg NMN sublinguals and took 7 of them per day a minimum of 1 hr. apart. We capsuled up the activator and took the entire daily dose 1st thing in the morning. The results were equal to the 1,000 mg/day of oral tablets.
Sept. 25, 2018 Oct.14, 2018 3 weeks NAD+ w/Act. 50 8 400 Sublingual Good
Got some 99.1% purity NAD+ and tabletted it up for sublingual use. I took 8 per day a minimum of 1 hour apart and took the activator dose first thing in the morning. Made enough for a 1-month supply but the arthritis came back so strongly that I quit taking the NAD+ after 3 weeks. This result was very surprising as our quest, as I understood it, was to raise NAD+ in the blood levels. Upon reviewing numerous research documents, I now believe that our quest is to raise intracellular NAD+ levels.
Oct. 15, 2018 Nov. 15, 2018 1 month NMN w/Act. 30 8 240 Sublingual Good
After researching as much as we could on sublingual dosing, saturation limits and cross membrane transport times we have come to believe that NMN is a great candidate for sublingual dosing. We also believe that the saturation limit is 15 to 25 mgs per application and the transport time is somewhere around 45 minutes. To test that theory we put together some 30 mg NMN sublinguals and took 8 per day with a minimum of 1 hour between dosings. I also took the daily activator dose first thing in the morning. After 1 month of this regimen we found that the resulting benefits were the same as the 50 mg, 7 times per day regimen.
Nov. 16, 2018 Dec. 2, 2018 3 weeks NMN only 30 8 240 Sublingual Good
Tried the same 30 mg NMN sublingual 8 times per day but without the activator and found that my arthritis had returned by the 3rd week.
Dec. 3, 2018 NMN w/Act. 400 2 800 Oral tablet
Dec. 3, 2018 NMN w/Act. 30 6 180 Sublingual
I have now started on what I believe will be my personal maximum benefits for minimum NMN dosing regimen. 400 mg of oral NMN with the activator twice per day interspersed with sublingual NMN 6 times per day. I will be going in for my full blood test after 3 months of this regimen. I will post the results when I receive them.
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