The latest stem cell protocol can be found in post #694, and a link to the latest can always be found on my profile page. The following mostly-flavonoid senolytic protocol seems to work fairly well, at least when one has topped off their stem cell pools. It is experimental, of course.
Senolytic protocol with stem cell replacement
This is where epigenetically old senescent cells are removed and replaced with epigenetically young cells derived from stem cells
Time 0 —
Curcumin (phytosome or liposome) — 2 g
Quercetin (phytosome or liposome) — 1 g
Apigenin — 100 mg
Resveratrol — 100 mg
Sodium butyrate — 1 g (then every ½ to 1 hour for 4 hours)
Time 4:00 —
Leucine — 5-10 g
Threonine — 10 g
I’ve also tried adding a gram of azithromycin at one or two hours, and that increases the effect somewhat. The butyrate (p38 activator) seems to make the biggest difference, but must be dosed several times as its half life is just a matter of minutes.
I’m working with the hypothesis that stem cells will be mobilized by apoptosis, thus the leucine (which stimulates satellite cells) and threonine (pluripotent cell nutrient).
Thanks for this, Turnbuckle. I notice that this is still shown as the latest version in your profile. Some questions, hopefully not already answered upthread:
1. How often can this be done? What interval is optimal? (I understand that you like to alternate it with your stem cell self-renewal protocol.)
2. What sort of creative prescription process is necessary to obtain azithromycin? I've found antibiotics difficult to obtain, even in otherwise dodgy countries. And what dose?
3. If we're to believe the mouse studies, then dasatinib plus quercetin is more effective than either alone. So why not add dasatinib? (I'm not sure whether or not I'm in favor of doing so, as the last thing I want to do is reduce competition to dormant tumor cells, even if that competition is senescent. On the other hand, senescence is also bad and can create its own tumor cells, eventually. A 2-year rat study showed a modest increase in tumor incidence with 100-plus-mg human-equivalent daily dose, but of course we're not going to repeat this daily for years, but then again we have many more years for the results of our errors to manifest as tumors. Unfortunately I lost the link.)