AGE REVERSAL PROTOCOL update
This is an update from post 1175. The major change is the addition of AAKG. After I added that, I felt there was definite acceleration in age reduction. As measured by Trume, my epigenetic age is now 22.6 years below my chronological age, a doubling of the decline in just 2 months. See this post. Since AKG is involved with TET-mediated DNA demethylation, it may be removing unnecessary methylation marks on older stem cells.
Caveats:
1. This is a work in progress.
2. It is intended as a geriatric treatment for age reversal.
3. One should avoid alcohol during this treatment.
4. A link to the latest protocol can always be found on my profile page.
Part 1: Stem cell self-renewal
Time 0 —
1Stearic acid — 5-10 g (food grade, in brownie)
Time 3:00 —
2Sulforaphane — 100 mg
3Liposomal glutathione — 1 g
4SAM-e — 500 mg
5AAKG — 2-3 g
Time 3:30 —
6C60 — 3 mg (in oil)
7Amino acids: Threonine — 2-3 g, Lysine — 2 g, Methionine — 1 g, Leucine — 1 g
Lysine and Methionine should be repeated as necessary every few hours.
Part 2: Senescent cell replacement (24 hours later or more)
7Lysine — 2 g, Methionine — 1 g
8Nicotinamide — 2g, Ribose — 2g
9Curcumin (liposomal or phytosomal) — 2g
Lysine and Methionine should be repeated as necessary every few hours.
A more aggressive senolytic protocol may be used from time to time for the resistant senescent cells that tend to build up with age. This would include —
10Azithromycin 500 mg
10Quercetin phytosome 250 mg
Notes:
(1) This is for mito fusion, which directs SCs to self-renewal. Food grade stearic acid is a waxy triglyceride with about 50% stearic acid moieties. It has a high melting point and will have very poor availability unless properly prepared. Baking it into brownies is one option: Using a box mix that calls for 1/2 to 2/3 cups of oil, eliminate the oil and add 120 grams of stearic acid flakes or granules, leaving the rest of the recipe unchanged. Mix at room temp using a power mixer, bake according to directions on the box, then divide 3x4 and freeze most of it for later use. Use ½ to 1 brownie for this protocol.
Mango butter is an option. Its melting point is so low it can be mixed into a cup of hot chocolate.
(2) Sulforaphane penetrates the BBB to produce mito fusion there, while stearic acid is blocked. 50 mg caps can be obtained from Amazon — Thorne Research - Crucera-SGS - Broccoli Seed Extract for Antioxidant Support - Sulforaphane Glucosinolate (SGS)
(3) Primary antioxidant.
(4) Methyl group donor.
(5) AAKG is arginine-alpha-ketoglutarate. Alpha-ketoglutarate facilitates TET-mediated DNA demethylation of stem cells.
(6) UCP2 blocker. A teaspoon of commercially available C60 oil contains about 3 mg.
(7) These can be used in caps or tablets, though I use a commercial blend of essential amino acids here, adding extra lysine, methionine and leucine. Pluripotent cells have a special need for these three. I would spread out the doses as indicated, to avoid nausea.
(8) Used together, nicotinamide and ribose rapidly increase NAD+, which drives mitochondrial fission, required for senescent cell apoptosis and replacement.
Niacin and ribose can alternatively be used at 1 g each. But this is only for people familiar with the niacin flush.
(9) Senolytic
(10) A pair of senolytics used to remove more resistant senescent cells. Hypothetically, these become more numerous with age.
A mathematical model, which for simplicity only uses two types of senescent cells (removable and non-removable), achieves an excellent fit to experimental data. Interestingly, our model also predicts a slowdown of senescent cell turnover with age, in our case explained by an accumulation of non-removable senescent cells relative to removable ones.
https://www.fightagi...lar-senescence/
Suggested frequency: One cycle every 10-14 days. Part 2 may be repeated more than once during that time, but not more than 3 days in a row.
Epigenetic testing:
Trume
Epiagingusa
MyDNAge
Edited by Turnbuckle, 01 December 2020 - 05:50 PM.