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Stem cell self-renewal with C60

c60 stem cells mitochondria fusion stearic acid aging hydroxytyrosol olive oil mct oil proliferation

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#541 Andey

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Posted 24 September 2018 - 06:27 AM

 

Given that things have plateaued subjectively, I’ve stopped the treatments for now and will get more epigenetic age tests over the next months. Below is my last protocol, with a couple of speculative items in red that I don’t have enough experience with to say if they really add (though they seem to)—

 

 

 

  I imagine not everybody experiencing all the expected benefits of a protocol. At least I could say this in my case as I haven't noticed a significant effect on wrinkles or the quality of my skin.  

Though after I moved protocol to a morning and fasted state I noticed some improvements like my gray hair patches decreased, could be some improvements for the skin but I've started red light therapy approx this time so it's hard to tell.

 Is there anything that you think would be the first suspect for intervening with a protocol that some people could do differently than you? 


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#542 Turnbuckle

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Posted 24 September 2018 - 07:54 AM

I wouldn't do red light therapy at the same time as C60 (on the same day, anyway), given that C60 is so sensitive to light and red light is so penetrating. That said, could you list exactly what you are doing?


Edited by Turnbuckle, 24 September 2018 - 08:22 AM.

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#543 lost69

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Posted 24 September 2018 - 10:26 AM

  I imagine not everybody experiencing all the expected benefits of a protocol. At least I could say this in my case as I haven't noticed a significant effect on wrinkles or the quality of my skin.  

Though after I moved protocol to a morning and fasted state I noticed some improvements like my gray hair patches decreased, could be some improvements for the skin but I've started red light therapy approx this time so it's hard to tell.

 Is there anything that you think would be the first suspect for intervening with a protocol that some people could do differently than you? 

i m also planning to use redlight for face skin, thyroid and thymus, how many days you use it?i checked research for thyroid and it was used twice a week

 

C60 halflife should be about 3 days if i remember correct



#544 Andey

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Posted 24 September 2018 - 11:41 AM

i m also planning to use redlight for face skin, thyroid and thymus, how many days you use it?i checked research for thyroid and it was used twice a week

 

C60 halflife should be about 3 days if i remember correct

 

  To be honest I am not that organized to plan it. It's just when a have a spare time at home. 

I have pretty small beam device https://www.amazon.c...duct/B071Z12H67 , so its usually one or two zones 2-3 minutes each.

Joovv creators propose a daily session duration to 10 min with irradiance or 100mW/c2 at 10cm. My device should be 142mW/c2 at 10cm. I use it a bit closer, but for a shorter period.

 

All concept of photobiomodulation is that it mimics the sun exposure. I 've read that its estimated that sun avoidance could decrease lifespan by up to 8years. Red light therapy devices gave around the same irradiance that a sun does during a day in those frequency bands (red, near IR and IR). My gut feeling is that light exposure in a living cell doesn't lead to the same C60 degradation as in an inert medium. A cell is maintaining homeostasis so ROS peaks would be contained in moderate values.



#545 Andey

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Posted 24 September 2018 - 12:14 PM

That said, could you list exactly what you are doing?

 

I 've started with a full mirror of the protocol at a time but now somewhat deviated from it.

2 days in a row during a weekend

 

  Time 0 Stearic acid (now it's around 32g of food grade Shea butter to reach target 10g of Stearic acid) + sunflower lecithin in a warm water to melt butter.

  Time 2H TUDCA 500mg, L-Threonine — 5-7 g, Taurine — 5 g, ALA — 600 mg, Time-release Vitamin C, + sometimes vitamin B complex

  Time 2.5 C60oo+sunflower lecithin mixed in the mouth.

 

  Time 3H - either do the first meal or go to do a workout than have a meal.

 

  Ive pushed amino acids closer to C60  because they absorb readily anyway and C60 in olive oil would get to bloodstream probably 1h+ after taking it.

 

BTW Is it safe to use C60 that have been stored in a powder form in a drawer for few years? 

P.S. I've looked into it and looks like a messed it up with such long shelf life

 

 


Edited by Andey, 24 September 2018 - 01:04 PM.


#546 orion22

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Posted 24 September 2018 - 01:15 PM

My latest protocol, my latest results, and a note of caution:

 

First the caution.

 

The hypothesis here is that

  1. Pools of endogenous stem cells can be refilled with this protocol, and
  2. Being to some degree unprogrammed (age zero), these stem cells can be used to replace old somatic cells and thereby lower epigenetic age.

The problem with part 2 is that adult stem cells do have some epigenetic programing, and thus the age of those programed genes cannot be brought down this way. In fact, by overdoing this protocol, one might actually age them to a degree much greater than somatic cells. The results from epigenetic testing are still too noisy to tell if this is happening or not. (See the results for 3 epigenetic tests at end of this post.)

 

Thus I suggest this protocol should be considered only for people 60+ years, and even then should be used judiciously. By that I mean using it only once a week or two, and not after effects have plateaued. (Of course I was too impatient to do stretch it out like that, but I’ve stopped now that the effects seem to have plateaued.) I advise getting an epigenetic test before and halfway through.

 

 


and

1 what if the ageing is due to the many errors from the fusion 

2 the way i understand it every time a division happens errors are created adn thats ageing  if you did so many in such short time could that "overload " and cause more errors similar to how if you run to many programs on a pc at same time will cause problems 

3 you sure this effect of "ageing" will happen on young people as well ?

4 why did the mice live longer if this things ages you?

5 any theory how to prevent this?

6 if you agree on 2 than could raising nad+ when you do the protocol prevent the ageing because that will decrease the number of errors (i know that promotes fission)


Edited by orion22, 24 September 2018 - 01:21 PM.


#547 Turnbuckle

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Posted 24 September 2018 - 02:02 PM

1 what if the ageing is due to the many errors from the fusion 

2 the way i understand it every time a division happens errors are created adn thats ageing  if you did so many in such short time could that "overload " and cause more errors similar to how if you run to many programs on a pc at same time will cause problems 

3 you sure this effect of "ageing" will happen on young people as well ?

4 why did the mice live longer if this things ages you?

5 any theory how to prevent this?

6 if you agree on 2 than could raising nad+ when you do the protocol prevent the ageing because that will decrease the number of errors (i know that promotes fission)

 

 

This is about nuclear DNA, not mtDNA. My concern is with adult stem cells and the degree to which they are not entirely unmethylated. Epimutations could thus creep into any genes that are not methylated de novo. Those genes could thus age epigenetically (on average) even if the others grew younger.

 

For this to make you younger, you need to replace epigenetically old somatic cells with epigenetically young somatic cells derived from stem cells. Thus replacing senescent cells with new cells will make a big difference. For someone very young who doesn't have any old cells to replace, this isn't going to work. So the value of it goes up with age, even as the speculative danger of aging stem cells goes down.


Edited by Turnbuckle, 24 September 2018 - 02:46 PM.

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#548 lost69

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Posted 24 September 2018 - 03:09 PM

  To be honest I am not that organized to plan it. It's just when a have a spare time at home. 

I have pretty small beam device https://www.amazon.c...duct/B071Z12H67 , so its usually one or two zones 2-3 minutes each.

Joovv creators propose a daily session duration to 10 min with irradiance or 100mW/c2 at 10cm. My device should be 142mW/c2 at 10cm. I use it a bit closer, but for a shorter period.

 

All concept of photobiomodulation is that it mimics the sun exposure. I 've read that its estimated that sun avoidance could decrease lifespan by up to 8years. Red light therapy devices gave around the same irradiance that a sun does during a day in those frequency bands (red, near IR and IR). My gut feeling is that light exposure in a living cell doesn't lead to the same C60 degradation as in an inert medium. A cell is maintaining homeostasis so ROS peaks would be contained in moderate values.

 i will PM about redlight because off topic



#549 lost69

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Posted 24 September 2018 - 03:18 PM

This is about nuclear DNA, not mtDNA. My concern is with adult stem cells and the degree to which they are not entirely unmethylated. Epimutations could thus creep into any genes that are not methylated de novo. Those genes could thus age epigenetically (on average) even if the others grew younger.

 

For this to make you younger, you need to replace epigenetically old somatic cells with epigenetically young somatic cells derived from stem cells. Thus replacing senescent cells with new cells will make a big difference. For someone very young who doesn't have any old cells to replace, this isn't going to work. So the value of it goes up with age, even as the speculative danger of aging stem cells goes down.

 

can we make your protocol for senescence cell clearance more potent by adding good putiry Piperlongumine from labs since no supplement is available yet?it looks like a potent senolytic in vitro

 

Thus replacing senescent cells with new cells will make a big difference

 

do we have some impact from senecent cells clearance even in the range 45-50yo?


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#550 Kentavr

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Posted 25 September 2018 - 06:41 PM

can we make your protocol for senescence cell clearance more potent by adding good putiry Piperlongumine from labs since no supplement is available yet?it looks like a potent senolytic in vitro

 

 
A good idea, because then we will do the directed process.
 
As far as I know, cells do not divide, if there is no place for them near them.
It would be nice if the cell was gone, and in its place would come not a somatic cell, but a stem cell.
 
Thus, this could increase the efficiency of the protocol.

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#551 Turnbuckle

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Posted 25 September 2018 - 07:42 PM

Piperlongumine looks like a good addition for apoptosis, as does sodium butyrate, which notably induces senescence and apoptosis in many tumor cell types.

 

I'm presently using for clearance of senescent cells (apoptosis)--

 

sodium butyrate -- 500 mg

quercetin -- 500 mg

IP6 -- 500 mg

fisetin -- 50 mg

apigenin -- 50 mg

 

Butyrate protects against muscle loss (sarcopenia) in mice. It also "greatly enhances the efficiency of induced pluripotent stem (iPS) cell derivation from human adult or fetal fibroblasts," which though not directly on point, suggests that it might have some usefulness in the stem cell protocol, in particular as it upregulates some pluripotency genes.

 

A convergent picture from both studies suggests that butyrate can facilitate cell fate changes by promoting epigenetic remodeling and the expression of pluripotency-associated genes, among other possible conserved mechanisms. It would be of great interest to test whether butyrate could reduce epigenetic barriers and enhance other types of epigenetic reprogramming such as transdifferentiation.

https://www.ncbi.nlm...les/PMC3015217/

 

 

It's not clear to me how it would be used with existent stem cells, however.

 

Kentavr: As far as I know, cells do not divide, if there is no place for them near them. It would be nice if the cell was gone, and in its place would come not a somatic cell, but a stem cell.

 

 

Exactly. Once the stem cell pools are full, it's time to make room for shiny new cells minted from stem cells. So old cells must be killed off.

 

 


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#552 Kentavr

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Posted 26 September 2018 - 06:26 PM

Exactly. Once the stem cell pools are full, it's time to make room for shiny new cells minted from stem cells. So old cells must be killed off.

 

The main thing is that in their place there is no nerve cells))

 

Turnbuckle, what protocol are you currently running?

 
The second question is: if you do not perform any protocol with C60, do you appear wrinkles over time?


#553 tolerant

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Posted 27 September 2018 - 11:51 AM

Hi everyone,

 

I am new to this thread. I'm relocating here from Turnbuckle's Alzheimer's protocol thread after he advised me that my continuously deteriorating cognitive impairment is not likely the result of AD, but could be (along with my continuously deteriorating severe treatment-resistant major depression, anxiety, and other psychiatric symptoms) the result of prolonged use antidepressants. This post from the AD thread and the few below it sum up my situation.

 

So I'm about to start getting together all the ingredients needed from the protocol. As discussed in the posts referenced above, I am interested in increasing the stem cell pool in the hippocampus, so my antidepressant medication can regain its effect of producing new neurons. As this is all brand new to me, I have a list of questions to Turnbuckle and anyone else who is able to help out:

 

1. Which protocol should I follow? Is it this one (from his profile page) or this (which is the latest in time and is the one identified in his response to me in the AD thread)? Do I need just the stem cell renewal, or satellite cell renewal and senescent cell clearance as well? Given that my hippocampal volume has been estimated to be within top 1% of population for my age, as discussed in this post, yet I am very ill mentally and cognitively, could this mean that a significant proportion of my hippocampal cells are indeed senescent?

 

2. I am very bad at cooking. I had to google to find out what a brownie is. When people say that stearic acid should be consumed with brownies, does it mean you need to cook the brownies yourself mixing in the stearic acid, or can you just purchase the brownies and stuff the stearic acid inside? And when it comes consuming it with hot chocolate, does it mean just buying your standard cocoa powder and mixing it in (with added lecithin for solubility)?

 

3. Turnbuckle appears to say here that I may try stearic acid on its own. So do I understand correctly that it is the "master ingredient" when it comes to enlarging your pool of stem cells? (I would assume that, judging by the name of this thread, C60 is also a must-have.) I am anxious to understand which ingredients are key and which ingredients are very much secondary (i.e. I might want to avoid, for the moment, go down the path of including every single substance that has been linked to stem cell renewal, because I've been down that road with depression and anxiety and trying substance after substance with no luck takes a great emotional toll on me).

 

4. I have access to some HGH, which I was planning to use for depression and cognition. There are a number of papers linking HGH and hippocampal neurogenesis (see, e.g., here), but they are too technical in that I can't place their conclusions neatly within the symmetrical/asymmetrical division paradigm. If I am looking to increase my pool of hippocampal stem cells by way of symmetrical division, and let the antidepressant medication, which helped me so much in the past, do the job of producing somatic cells by way of asymmetrical division, is taking HGH advisable?

 

Thanks in advance to everybody who can help me out with my protocol.



#554 Turnbuckle

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Posted 27 September 2018 - 02:33 PM

@tolerant

 

Looking back at what you said before, I don't think this is right for you any more than an AD treatment would be right for you. You have not complained about memory issues, and in fact you say that your hippocampal volume puts you in the top 1% of your age group. So those things say that stem cell treatments are probably not right for you either. I suggest you look into the brain health threads on this site for a more likely avenue to explore. And since you apparently had a brain scan, you should also consider amygdala enlargement, which can be the source of mental issues.


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#555 Fafner55

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Posted 27 September 2018 - 03:21 PM

NSI-189 is synergistic with the Turnbuckle stem cell protocol

 

In November 2015 I self-experimented with 30 mg NSI-189, 6 days a week for 4 weeks. After a mild headache for the first few days, there were benefits. I was more verbal, more productive at work and experienced noticeably increased skin/touch sensitivity. After about 3 months, those benefits returned to basal levels.

 

Recently I took 40 mg NSI-189 daily for 3 days coincident with 3 mg C60-MCT oil 1x/day and 10 gm stearic acid 2x/day. The effect was much, much stronger than before. Nights were restless and filled with vivid dreams. During the days I felt jittery and edgy, similar I image to drinking 4 or 5 shots of espresso. On day 3 I was lost in my thoughts. On day 5, my sense of smell was unusually acute: I was annoyed to smell a dog park a block away and paint from a construction site 3 blocks away. I was impatient with myself and others. Overall, this was not a pleasant experience. Maybe 20 or 30 mg/day for just 2 days with the Turnbuckle protocol would be a better dose for stimulating neurogenesis.


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#556 Andey

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Posted 27 September 2018 - 06:51 PM

NSI-189 is synergistic with the Turnbuckle stem cell protocol

 

In November 2015 I self-experimented with 30 mg NSI-189, 6 days a week for 4 weeks. After a mild headache for the first few days, there were benefits. I was more verbal, more productive at work and experienced noticeably increased skin/touch sensitivity. After about 3 months, those benefits returned to basal levels.

 

Recently I took 40 mg NSI-189 daily for 3 days coincident with 3 mg C60-MCT oil 1x/day and 10 gm stearic acid 2x/day. The effect was much, much stronger than before. Nights were restless and filled with vivid dreams. During the days I felt jittery and edgy, similar I image to drinking 4 or 5 shots of espresso. On day 3 I was lost in my thoughts. On day 5, my sense of smell was unusually acute: I was annoyed to smell a dog park a block away and paint from a construction site 3 blocks away. I was impatient with myself and others. Overall, this was not a pleasant experience. Maybe 20 or 30 mg/day for just 2 days with the Turnbuckle protocol would be a better dose for stimulating neurogenesis.

 

  It looks more like neurons was overexcited. It could be a good thing but a bad one too.

  Good if they grow few millimeters along and those endings are not yet protected by myelin shielding. That's what people experience when neurons regrow after a trauma.

  But neurons overexcite too when they are damaged. To add up, its dangerous to overexcite neurons for too long, they die, that's how glutamate neurotoxicity works, its literally excitotoxicity.

 

  I've noticed it too, but without NSI-189. Sometimes I could hear my quartz wrist watch tick 2 meters away. Usually, it's 3-5cm max. For me its a bit concerning)


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#557 tolerant

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Posted 28 September 2018 - 07:32 AM

@tolerant

 

Looking back at what you said before, I don't think this is right for you any more than an AD treatment would be right for you. You have not complained about memory issues, and in fact you say that your hippocampal volume puts you in the top 1% of your age group. So those things say that stem cell treatments are probably not right for you either. I suggest you look into the brain health threads on this site for a more likely avenue to explore. And since you apparently had a brain scan, you should also consider amygdala enlargement, which can be the source of mental issues.

 

I haven't complained about memory issues? Maybe I have been using the term "cognitive impairment" or suchlike. I have horrendous memory issues. For example, I may brush my teeth and five minutes later forget whether I have in fact brushed my teeth. So I go to the bathroom and feel the bristles of my toothbrush. If they are wet, I know that I have brushed my teeth. Or to use your own example, I have great difficulty following plots of TV shows or movies. In fact, it is so taxing and painful that I hardly ever watch TV at all. When I was trying to watch the World Cup, I would have to remind myself every five minutes or so which way a team is kicking, left or right. 

 

Yes, I had two scans, and when I was able to run FDA-approved morphometric analysis on those scans. One scan put my hippocampus volume on the 80th percentile and the other on the 99th. So it appears likely that I have a larger-than-average hippocampus, but even the difference between the two scans confirms that brain morphometry is not an exact science. The reports also contained the volume of my amygdala but did not specify any sort of percentile or average. But looking at studies which measure the amygdala, it seems that my amygdala is also considerably larger than average. But it's not like I can take anything to reduce it. There appear to be some studies that suggest that mindfulness meditation can decrease the size of the amygdala, but I have tried mindfulness one hundred times and didn't get anywhere with it.

 

I have looked at Brain Health threads, but there really isn't in there that I haven't tried or have the energy to try. I mean, I have tried over 100 substances for my anxiety and depression, most of which are backed up by studies and haven't got anywhere. I am sure there are 100 more substances backed up by studies which I haven't tried, but my mind refuses to go down that track, partly because of impaired cognition.  I think I will try stearic acid and C60. Heaps of people in this thread appear to be doing it for fun, so I don't think I have anything to lose by trying. And the theory behind my psychiatric and cognitive health issues which you put forward is very elegant and really could explain everything that's been happening during the last six-and-a-half years.


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#558 Turnbuckle

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Posted 28 September 2018 - 09:55 AM

I have tried over 100 substances for my anxiety and depression, most of which are backed up by studies and haven't got anywhere. I am sure there are 100 more substances backed up by studies which I haven't tried, but my mind refuses to go down that track, partly because of impaired cognition.  I think I will try stearic acid and C60. Heaps of people in this thread appear to be doing it for fun, so I don't think I have anything to lose by trying.

 

 

I doubt if anyone is trying this for fun. People here seriously want to live longer and this is a treatment to reverse aging. So again, this doesn't seem right for you, and I suggest you look at your genetic results first before rushing down yet another wrong path. Why try hundreds of treatments randomly when a simple test could nail it down for you?


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#559 tolerant

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Posted 28 September 2018 - 10:04 AM

Well I didn't mean "for fun" literally. Which test would nail it down? 23andme?

#560 Turnbuckle

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Posted 28 September 2018 - 10:20 AM

Well I didn't mean "for fun" literally. Which test would nail it down? 23andme?

 

Absolutely. I'm surprised you haven't already ordered it. 


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#561 Fafner55

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Posted 28 September 2018 - 12:33 PM

Well I didn't mean "for fun" literally. Which test would nail it down? 23andme?

 

After a 23andMe test, you can upload the result file to https://promethease.com/ for a comprehensive analysis for just $5 or so.


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#562 lost69

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Posted 28 September 2018 - 01:10 PM

Piperlongumine looks like a good addition for apoptosis, as does sodium butyrate, which notably induces senescence and apoptosis in many tumor cell types.

 

I'm presently using for clearance of senescent cells (apoptosis)--

 

sodium butyrate -- 500 mg

quercetin -- 500 mg

IP6 -- 500 mg

fisetin -- 50 mg

apigenin -- 50 mg

 

Butyrate protects against muscle loss (sarcopenia) in mice. It also "greatly enhances the efficiency of induced pluripotent stem (iPS) cell derivation from human adult or fetal fibroblasts," which though not directly on point, suggests that it might have some usefulness in the stem cell protocol, in particular as it upregulates some pluripotency genes.

 

 

It's not clear to me how it would be used with existent stem cells, however.

 

 

Exactly. Once the stem cell pools are full, it's time to make room for shiny new cells minted from stem cells. So old cells must be killed off.

 

did the protocol on weekend, no fatigue except 15-30min after taking it (experienced brain fog, dizzyness, slow reaction time).main effect was big blisters around the mouth which spread after shaving and a herpes on lips.blisters are still there and annoying (antibiotic cream no effect)

 

the best part is effect on HRV parameters, they were stable for a very long time and now day after day improving, this could be the protocol or increasing gdf11 dose (gdf11 dose was increased early sept and response is usually very fast so not probable):

baseline about hrv 48 rmssd 22 now increasing day by day to 52 and rmssd 29

 

lets see if rmssd keeps this trend, this is the most interesting parameter and it was not responding by increasing to gdf11 anymore

 

i ll add sodium butyrate -- 500 mg while for Piperlongumine not really sure it is super expensive on sigma and others

 

i guess this senolytic protocol must be used once a month to allow stemcell to recover the damage, correct?or we can attempt more treatments?


Edited by lost69, 28 September 2018 - 01:12 PM.


#563 tolerant

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Posted 28 September 2018 - 01:19 PM

Absolutely. I'm surprised you haven't already ordered it. 

 

 

After a 23andMe test, you can upload the result file to https://promethease.com/ for a comprehensive analysis for just $5 or so.

 

I had a look at a sample report and FAQ on the 23andme site. I can see that it tells you your relative probability of developing certain diseases (which I'm not sure I want to know), and what kind of antidepressants I am more likely to respond to (which is not really relevant because I've tried them all). Will it tell me whether it is a good idea to try to stimulate hippocampal neurogenesis by using the protocol set out in this thread? 

 

I understand that most people in this thread are here because they want to stay young and healthy, whereas I have a grave problem and am frantically trying to fix myself. For me, time is of the essence. If I am unable to rescue at least one of (a) my rapidly deteriorating cognition or (b) my rapidly deteriorating mental health, I will end up tied up in the "official" psychiatric system, which has so far totally failed me over the course of six years. And that could be any day now, really. If that eventuates, my future is likely to be very grim. The methods being discussed in this thread are a generation away from entering mainstream medicine, so it's a case of now or never for me.


Edited by tolerant, 28 September 2018 - 01:23 PM.

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#564 Turnbuckle

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Posted 28 September 2018 - 01:34 PM

 

 

i guess this senolytic protocol must be used once a month to allow stemcell to recover the damage, correct?or we can attempt more treatments?

 

 

After more than seventy stem cell treatments with only the occasional senolic treatment, I began doing senolic treatments once a day. I've done them for a week and will probably continue for another week. I found the most noticeable effect several hours afterward, and this could be increased by taking potassium nitrate and going to the gym at that time. Apoptosis, exercise and supplemental nitrate ought to act synergistically to stimulate satellite cells. And this appears to be the case as I'm getting nice results from it.


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#565 tolerant

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Posted 29 September 2018 - 10:04 AM

Has anybody following the protocol in this thread experienced mental health or cognitive benefits? If so, can you please briefly describe your experience?

#566 Fafner55

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Posted 29 September 2018 - 04:32 PM

As autophagy is essential for maintaining stem cell health and stemness, I started a thread Autophagy Induction with Liposomal Trehalose that might be relevant to this one.

“Autophagy maintains the metabolism and function of young and old stem cells” (2017) https://www.nature.c...les/nature21388

 

 


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#567 Kentavr

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Posted 29 September 2018 - 07:39 PM

Has anybody following the protocol in this thread experienced mental health or cognitive benefits? If so, can you please briefly describe your experience?

 

 

In experiments in mice as well as in humans, the drug metformin activates the expression of the AMPK gene, which in turn affects aPKC / CBP and mobilizes stem cells, thereby creating new neurons (cells of the nervous system and brain). The stem cells of mice receiving the metformin drug produce neurons two times more potent. This leads to an increase in the number of new neurons in the hippocampus by 30%. The hippocampus is a region of the brain that actively participates in the formation of new memories. Indeed, as shown by experiments, the ability to form new memories in experimental mice significantly improves.
 
In order to obtain this effect, it is sufficient to consume about 1000 mg of metformin per day for a person weighing 60 kg.
 
 
 (Russian language)
 
Important note:
 
1. If you take metformin, take 2 hours before admission, take vitamin B12 in the form of methylcobalamin (!). For example, Methyl-B12 1000 mg sublingually from Jarrow Formulas
 
2. After taking vitamin B-12, do not take antioxidants for at least 10-12 hours.
 
The reason is as follows:
 
metformin - destabilizes membranes of defective mitochondria.
antioxidants - stabilize the membranes of any mitochondria.
 
If you do not observe the interval between doses, the effect of metformin will be minimal.
 
3. Choose metformin with sustained release. For example, Glucophage Long.

Edited by Kentavr, 29 September 2018 - 07:49 PM.

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#568 tolerant

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Posted 30 September 2018 - 11:37 PM

Absolutely. I'm surprised you haven't already ordered it. 

 

 

After a 23andMe test, you can upload the result file to https://promethease.com/ for a comprehensive analysis for just $5 or so.

 

So I am about to order 23andMe. The only product that they have is called Ancestry. Is this right? Is that what I need? Thanks.


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Posted 30 September 2018 - 11:50 PM

My latest protocol, my latest results, and a note of caution:

 

First the caution.

 

...

 

 

Thus I suggest this protocol should be considered only for people 60+ years, and even then should be used judiciously. ... I advise getting an epigenetic test before and halfway through.

 

 

Stem cell self-renewal, with C60

 

Time 0 —

Stearic acid — 10 g (in hot chocolate or brownie)

 

...

 

Some questions if you don't mind:

 

1. What are the risks associated with following the protocol for people under 60?

 

2. What website would you recommend for doing an epigenetic test?

 

3. Is the rationale for mixing stearic acid in hot chocolate that hot chocolate itself contains stearic acid.

 

4. I'm sorry if this has been addressed already, but since, as you say, most stearic acid out there, as well as cocoa, is half palmitic acid, and since, for the purposes of this protocol, palmitic acid acts in the opposite way to stearic acid, does this protocol still work with stearic acid that is half palmitic acid?

 

Thanks.


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#570 orion22

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Posted 01 October 2018 - 06:55 PM

by the way what about the antioxidant effect of c60 would taking 500ml of mct c:8 oil(the mct c8 Caprylic Acid) with c60 in it every day give you a insane boost?that would be like 50 million orac antioxidants every day also im making my c60 in c8 oil  hope thats ok 


Edited by orion22, 01 October 2018 - 06:56 PM.






Also tagged with one or more of these keywords: c60, stem cells, mitochondria, fusion, stearic acid, aging, hydroxytyrosol, olive oil, mct oil, proliferation

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