32 replies to this topic

[GreenPower]

After the most interesting results of the recent TRIIM study I've been reading up on the subject.

 

First I was kind of overwhelmed with the requirements, time and money needed for trying to replicate and/or incorporate the protocol in my own regimen. Then I started to feel it's actually a challenge .

 

I've therefore started to draft a regimen based on the TRIIM Protocol which I think I might be able to start in a month or so. Currently I'm collecting the necessary substances and tests needed. I've excluded HGH (Omnitrope) due to its lack of easy availability and will instead include some other substances which I think might be beneficial. From a testing point of view there will be no MRI's but some other tests with an emphasis on telomeres.

 

Because the the modified protocol is kind of ambitious I wonder if you see any obvious mistakes or easy improvements to it.

 

Below I've added is my first draft.

 

TRIIM Protocol without HGH and with some additional modifications

HGH has been left out because it seem to be a bit hard to obtain.

Pregnenolone has been included on a hunch.

Astragalus has been included since I'm currently using it (baseline/what I'm using today: once per day in the morning).

Gingko Biloba has been included since I'm currently using it (baseline: 1-3 times per week, usually around lunch).

 

Besides the substances below I consider the following to be included:

- Training sessions 2-3 times per week (baseline: currently 1 time per week)

- Regular saunas 4-5 times per week in order to decrease heavy metals (baseline: 1 time per half year)

- Transcendental Meditation at least 4-5 times per week around lunch (baseline: non-existent)

- Lower environmental stress (baseline: quite high)

- Low Carb High Fat diet (baseline: I'm currently in ketosis only one week every month or so)

 

1st week

Baseline tests

• Baseline blood tests: "Healthcheck XL+" with added Zinc, Cortisol, DHEAS, Thyroid tests. All tests during the morning.
• Baseline blood test: myDNAge Epigenetic Aging Clock test, around lunch
• Baseline blood test: RepeatDX 6-Panel assay Telomere length test, around lunch.
• Baseline blood test: LifeLength TAT, around lunch
• Baseline Saliva tests: Cortisol (morning), DHEA (morning)
• Baseline Heavy Metal Urine Test: 17 substances (Aluminum, Antimony, Arsenic, Lead, Cadmium, Chromium, Cobalt, Copper, Nickel, Palladium, Mercury, Iron, Platinum, Silver, Thallium, Zinc and Pewter), morning.
• uBiome Five Site bacterial tests: Gut, Mouth, Nose, Genitals, Skin)

 

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

2nd week

Adjustment tests

Blood

• CRP, Hb, LPK, TPK, MCH, MCHC, MCV, EPK, EVF
• DHEAS
• HbA1c (glycated hemoglobin)
• Glucosis
• C-Peptide

Saliva

• Cortisol (morning), DHEA (morning)

 

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

3rd week

Morning

2 x 25 mg DHEA (dose change to 50 mg if adjustment test results support this)

1 x 100 mg Pregnenolone (dose change to 100 mg if adjustment test results support this)

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1 x 500 mg Glucophage (Metformin)

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

4th week

Adjustment tests

Blood

• CRP, Hb, LPK, TPK, MCH, MCHC, MCV, EPK, EVF
• DHEAS
• HbA1c (Glycated hemoglobin)
• Glucosis
• C-Peptide

Saliva

• Cortisol (morning), DHEA (morning)

 

Morning

2 x 25 mg DHEA (dose change if adjustment test results support this)

1 x 100 mg Pregnenolone (dose change if adjustment test results support this)

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1 x 500 mg Glucophage (Metformin)

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

5th to 52nd week

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1 x 500 mg Glucophage (Metformin)

1 x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

Week 53

Tests for results

• Blood tests: Healthcheck XL+ with added Zinc, Cortisol, DHEAS, Thyroid tests. All tests during the morning.
• Blood test: myDNAge Epigenetic Aging Clock test, around lunch.
• Blood tests: RepeatDX 6-Panel assay Telomere length test, around lunch.
• Blood test: LifeLength TAT, around lunch.
• Saliva tests: Cortisol (morning), DHEA (morning)
• Heavy Metal Urine Test: 17 substances  (Aluminum, Antimony, Arsenic, Lead, Cadmium, Chromium, Cobalt, Copper, Nickel, Palladium, Mercury, Iron, Platinum, Silver, Thallium, Zinc and Pewter), morning.
• uBiome Five Site bacterial tests: Gut, Mouth, Nose, Genitals, Skin

 

[Kentavr]

HGH is available. It is used by bodybuilders. There are even instructions on YouTube how to inject it with a syringe.

However, if you do not want to use growth hormone, you can try using Ubiquinol in an amount of 200-300 mg. It will stimulate the production of ATP, which approximately can be taken as an acceleration of metabolism.

[Kentavr]

There is one feature when taking ubiquinol: its effect is not immediately felt.

It is necessary to drink at least 1 month to feel the powerful effect. And you have to take it every day.

Only the effect can be a little smaller. Growth hormone works after injecting for 2 hours, then its effect drops sharply. I think that the feature of the TRIIM protocol is this: when IGF-1 was reduced using metformin, we inject growth hormone at that moment. In this case, forced cell division occurs with reduced IGF-1. A decrease in IGF-1 gives increased expression of FOXO1, which stimulates the symmetrical division of its own stem cells.

[Kentavr]

I’m afraid without growth hormone it won’t work. But you can try to stimulate the division with the help of Ubiquinol. But it will be a constant weak "pressure", and not a strong "pressure" for 2 hours.

[Kentavr]

Yes, and again: it seems that the activation of telomerase accelerates epigenetic drift.

You can get the opposite effect - the acceleration of epigenetic hours due to the activation of telomerase.

[Kentavr]

What drugs would I include in addition?

L-threonine is likely to facilitate the division of specific stem cells.

Before proceeding to your protocol, I advise you to fully read the topic:

https://www.longecit...newal-with-c60/

There you will find information on L-threonine.

[Kentavr]

In addition, I would increase the dosage of metformin from 500 mg to 650 mg, or 750 mg.

Probably, in this case the effect will be stronger (because the decrease in IGF-1 will be more significant).

[Fafner55]

10 to 15 mg / day MK-677 at bedtime should sufficiently increase hGH. MK-677 is readily available on the internet without prescription.

1.  “Effects of an Oral Ghrelin Mimetic on Body Composition and Clinical Outcomes in Healthy Older Adults: A Randomized Trial” (2008)  http://annals.org/article.aspx?articleid=743451
2. “MK-677, an Orally Active Growth Hormone Secretagogue, Reverses Diet-Induced Catabolism” (1998) https://academic.oup.com/jcem/article/83/2/320/2865101
3. “Two-Month Treatment of Obese Subjects with the Oral Growth Hormone (GH) Secretagogue MK-677 Increases GH Secretion, Fat-Free Mass, and Energy Expenditure” (1998) http://press.endocrine.org/doi/full/10.1210/jcem.83.2.4539
4. “Adult GH deficiency throughout lifetime” (2009) http://eje-online.org/content/161/suppl_1/S97.full
5. “Evening versus morning injections of growth hormone (GH) in GH-deficient patients: effects on 24-hour patterns of circulating hormones and metabolites” (1990) https://www.ncbi.nlm.nih.gov/pubmed/2294131

 

[GreenPower]

I think MK-677 might be the most interesting option.

- It seem to mimic HGH.

- It can be taken orally (no needles).

- It seem to have been widely used without serious side-effects reported.

- High glucose levels and insulin resistance have been reported, but this should be handled by adhering to a Low Carb High Fat diet in combination with Metformin.

- Does not affect cortisol levels.

 

It understand it to be sort of legal, although not many serious vendors in Europe seem to sell it. I suppose this is because it's still experimental and also considered forbidden for usage in sports.

 

If I get hold of it, I will try to go with 15mg/day. It seem to be well below the maximum limit of 25mg/day which some sites seem to recommend.

[lukas_93]

MK-677 sounds like a great idea! If you are located in Europe, importing Omnitrope from Russia might also be an approach. Rupharma sells HGH as Getropin for example. Getting it through customs might be a problem, though. 

[Brundel]

For increased HGH levels Impamorelin and cjc works exceptionally well.

100mcg of each 5 days a week is enough.

GH secretagogues.

Relatively inexpensive online at bodybuilding research chem sites.

[Fafner55]

 

10 to 15 mg / day MK-677 at bedtime should sufficiently increase hGH. MK-677 is readily available on the internet without prescription.

 

 

10 mg MK-677 is not enough. I took that dose before bed for more than 2 weeks then had an early morning blood test. The results showed a hGH level of nearly zero. Since then I upped the dose to 20 mg. I am 64 years old.

[Fafner55]

Here are additional references for MK-677 and other growth hormone secretagogues including Impamorelin.

 

“Effect of Alendronate and MK-677 (a Growth Hormone Secretagogue), Individually and in Combination, on Markers of Bone Turnover and Bone Mineral Density in Postmenopausal Osteoporotic Women” (2001) https://academic.oup.com/jcem/article/86/3/1116/2847590/

 

“The Safety and Efficacy of Growth Hormone Secretagogues” (2017) https://www.smr.jsexmed.org/article/S2050-0521(17)30032-X/pdf

[Brundel]

10 mg MK-677 is not enough. I took that dose before bed for more than 2 weeks then had an early morning blood test. The results showed a hGH level of nearly zero. Since then I upped the dose to 20 mg. I am 64 years old.

 

Have you retested?

Where did you obtain your Mk-677? Lots of the research stuff is fake.

One of the benefits of Ipamorelin and CJC is the right dr can script it for you. Its sort of expensive but nothing compared to serostim or the like.

I think I pay 475$ for 75 days worth. Aside from its use in the TRIIM protocol I can show from labs that its very effective and I can say anecdotally that its effects are noticeable.

Muscle and strength does rise and bodyfat drops over the course of the first round of treatment.

 

[Fafner55]

Have you retested?

Where did you obtain your Mk-677? Lots of the research stuff is fake.

One of the benefits of Ipamorelin and CJC is the right dr can script it for you. Its sort of expensive but nothing compared to serostim or the like.

I think I pay 475$ for 75 days worth. Aside from its use in the TRIIM protocol I can show from labs that its very effective and I can say anecdotally that its effects are noticeable.

Muscle and strength does rise and bodyfat drops over the course of the first round of treatment.

 

I have not retested since upping the dose to 20 mg.

 

Directly testing hGH is not particularly reliable in the sense that it can vary widely within a day. In hindsight, I should have tested for IGF-1 instead as most researchers did in the papers I cited. Next time I'll test for both hGH and IGF-1.

 

I don't doubt that MK-677 is effective. Even at 10 mg when I started, I could sense firmer muscle tone and some aches in the joints of my hands within the first week. The mimor aches subsided within a week as my body adjusted to the changes.

[Smith]

I also agree that without some form of HGH the protocol will not likely work.  The whole point of HGH was rejuvenation of the Thymus, w/o which, the major benefit is lost.

 

MK-677 should work as a substitute, as it is an analog of HGH with even fewer side effects and is known to boost IGF-1.  The question remains the dosing.   5mg might be enough to boost IGF-1 levels, but without testing, its hard to know.  Hope someone can post their test results, or point out where one can obtain inexpensive IGF-1 blood tests.

 

EDIT: Found that you can get IGF-1 blood test for $69 and IGF-1 + HGH test for $97 at UltaLabTests.  You can also find some coupon codes to drop the price even further.

Edited by Smith, 12 November 2019 - 08:55 PM.

[William Sterog]

Well. Reliable source af MK-667 in Europe? It is worth it to try this protocol at 27 if you already feel like shit?

[aribadabar]

It is worth it to try this protocol at 27 if you already feel like shit?

 

Your thymus is involuting since puberty so chances are it can benefit from restoration even at 27. You just have less work to do than older people 

[Rocket]

There are high secretogogue peptides widely available and legal. Ghrp2 is my favorite. 3 to 4 small pins a day is equivalent to +2iu of hgh. I have done blood tests on hgh and Ghrp2 and can attest to this.

[Smith]

Good source for MK-677, with analysis reports showing purity levels.

 

Is anyone reproducing the TRIIM study protocol?  I'm surprised so few people are trying it out considering the amazing and well documented human results that were published. Seems most people are more interested in NAD+ precursors.

Edited by Smith, 18 December 2019 - 04:48 AM.

[William Sterog]

I don't like that they use ethanol in their solutions.

[Smith]

I don't like that they use ethanol in their solutions.

Whats wrong with ethanol?  Its in all alcoholic beverages and many medications such as nyquil.

 

The amount of ethanol you would ingest using the MK-677 above would be negligible.

[William Sterog]

Whats wrong with ethanol? Its in all alcoholic beverages and many medications such as nyquil.

The amount of ethanol you would ingest using the MK-677 above would be negligible.

I am an ex-alcoholic. In any case, I don't understand the necessity of using it.

[Raphy]

Good source for MK-677, with analysis reports showing purity levels.

 

Is anyone reproducing the TRIIM study protocol?  I'm surprised so few people are trying it out considering the amazing and well documented human results that were published. Seems most people are more interested in NAD+ precursors.

Yes I am surprised that no one seems interested by this protocole....

[Kentavr]

Yes I am surprised that no one seems interested by this protocole....

Honestly, I am also very surprised. A lot of people want to really increase their lifespan, and this protocol is a great chance. I am at a loss why people do not seek to simply repeat it.

[Raphy]

Honestly, I am also very surprised. A lot of people want to really increase their lifespan, and this protocol is a great chance. I am at a loss why people do not seek to simply repeat it.

Yeah....

So to add to this topic, I've contacted Intervene Immune to follow this protocol with them remotely (using a local doctor). Because ideally I'd like them to guide my dosage. They are too busy right now to deal with that unfortunately, so I'm looking to start with something like MK-677. We'll see how it goes.

[Paravani]

Intervene Immune is seeking volunteers for their ongoing TRIIM-X study, but there's a BIG catch -- because they're having trouble finding investors to finance the study, volunteers who are accepted into the study must pay for their own treatment, to the tune of about $18k per year.

However, because Intervene Immune has contracted with a national chain of clinics and labs, volunteers will not need to be located in or travel to southern California to participate in the study. Volunteers for this study will be located all over the US and may enter the study at any time.

So if you happen to have an extra $18k or more to finance your participation, you too can join the TRIIM-X study.

Please keep us updated if you do.

[rodentman]

Intervene Immune is seeking volunteers for their ongoing TRIIM-X study, but there's a BIG catch -- because they're having trouble finding investors to finance the study, volunteers who are accepted into the study must pay for their own treatment, to the tune of about $18k per year.

However, because Intervene Immune has contracted with a national chain of clinics and labs, volunteers will not need to be located in or travel to southern California to participate in the study. Volunteers for this study will be located all over the US and may enter the study at any time.

So if you happen to have an extra $18k or more to finance your participation, you too can join the TRIIM-X study.

Please keep us updated if you do.

 

The $18K was just too much for me, so I had to drop out.  I've since started doing it on my own.

[Paravani]

The $18K was just too much for me, so I had to drop out. I've since started doing it on my own.

Please elaborate. What dosages, timing, modifications are you using?

I've apparently stumbled onto a similar protocol that relies on increased physiological levels of IGF-I. I've been taking 600mg of Co-Q10 (as ubiquinol) daily, which according to this Swedish study increases IGF-I.

https://www.ncbi.nlm...les/PMC5469470/

Participants in the study took 200mg daily over 4 years. Average age was 76, and over the course of the study, mortality among those taking placebo was 4% higher than active participants. Cardio health showed significant improvement, even ten years after the end of the study, with reduced all-cause mortality.

I am 60, and I started taking 600mg ubiquinol about a month ago, in addition to a comprehensive regimen of daily vitamins and supplements that is detailed in my profile.

I have no way of measuring the specific levels of IGF-I in my blood at any time, but I can state that over the first month of treatment with ubiquinol, inflammation has been greatly reduced in my joints and my lungs.

I no longer have pain in my fingers or in my knees. Previously, knee pain was occasionally so severe that it interrupted my sleep.

More spectacularly, my chronic asthma, which had been so severe it required the use of a rescue inhaler six times daily and was not responding to steroid inhalers, is nearly gone. I can BREATHE!!!

My overall flexibility has also increased. I move easily now, no longer bent over when I walk, no longer unfolding myself carefully when I rise from my chair.

My husband, who is 65, has also begun taking ubiquinol. His overall health has always been excellent, but he has also noticed improvement. He wakes up without stiffness now, unless he has overexerted himself the day before (as he did on Sunday). His rate of recovery from overexertion has improved remarkably -- he woke this morning without any pain or stiffness, even after sleeping on his back.

A week ago we added 100mg DHEA to our daily regimen.

Because ubiquinol increases IGF-I, which increases insulin sensitivity, we are not concerned about our blood sugar levels. We both feel extremely energetic and alert.

We are, however, falling asleep earlier and sleeping more soundly. Although we aren't sleeping quite as many hours as we did when young, we both feel very well rested.

This week we will add regular exercise to our protocol, to increase our HGH levels.

This month I am due for my annual lung MRI. I'm very interested to learn what it shows about the state of my lungs now, and how they have improved since last year. (I will also ask the tech if the MRI also shows my thymus.)

Two days ago, I increased my ubiquinol dose to 900mg daily. I am curious to see if an increased dose might trigger other effects, like changes in hair growth or skin texture?

As a newbie to the forum, I am only allowed 5 posts daily. I will therefore only post one reply to this thread per day, at most. If you have questions that need an earlier response, you may PM me.

Edited by Paravani, 16 November 2021 - 12:18 PM.

[rodentman]

Please elaborate. What dosages, timing, modifications are you using?

I've apparently stumbled onto a similar protocol that relies on increased physiological levels of IGF-I. I've been taking 600mg of Co-Q10 (as ubiquinol) daily, which according to this Swedish study increases IGF-I.

https://www.ncbi.nlm...les/PMC5469470/

Participants in the study took 200mg daily over 4 years. Average age was 76, and over the course of the study, mortality among those taking placebo was 4% higher than active participants. Cardio health showed significant improvement, even ten years after the end of the study, with reduced all-cause mortality.

I am 60, and I started taking 600mg ubiquinol about a month ago, in addition to a comprehensive regimen of daily vitamins and supplements that is detailed in my profile.

I have no way of measuring the specific levels of IGF-I in my blood at any time, but I can state that over the first month of treatment with ubiquinol, inflammation has been greatly reduced in my joints and my lungs.

I no longer have pain in my fingers or in my knees. Previously, knee pain was occasionally so severe that it interrupted my sleep.

More spectacularly, my chronic asthma, which had been so severe it required the use of a rescue inhaler six times daily and was not responding to steroid inhalers, is nearly gone. I can BREATHE!!!

My overall flexibility has also increased. I move easily now, no longer bent over when I walk, no longer unfolding myself carefully when I rise from my chair.

My husband, who is 65, has also begun taking ubiquinol. His overall health has always been excellent, but he has also noticed improvement. He wakes up without stiffness now, unless he has overexerted himself the day before (as he did on Sunday). His rate of recovery from overexertion has improved remarkably -- he woke this morning without any pain or stiffness, even after sleeping on his back.

A week ago we added 100mg DHEA to our daily regimen.

Because ubiquinol increases IGF-I, which increases insulin sensitivity, we are not concerned about our blood sugar levels. We both feel extremely energetic and alert.

We are, however, falling asleep earlier and sleeping more soundly. Although we aren't sleeping quite as many hours as we did when young, we both feel very well rested.

This week we will add regular exercise to our protocol, to increase our HGH levels.

This month I am due for my annual lung MRI. I'm very interested to learn what it shows about the state of my lungs now, and how they have improved since last year. (I will also ask the tech if the MRI also shows my thymus.)

Two days ago, I increased my ubiquinol dose to 900mg daily. I am curious to see if an increased dose might trigger other effects, like changes in hair growth or skin texture?

As a newbie to the forum, I am only allowed 5 posts daily. I will therefore only post one reply to this thread per day, at most. If you have questions that need an earlier response, you may PM me.

 

I spoke about this with Dr Fahy quite a bit.  He still doesn't honestly understand the mechanisms that caused the dramatic improvements but believes they go way beyond just re-growing the thymus.  I just decided to use a generic lyophilized rHGH that claimed 99% purity. I had the product group-tested a few months ago, and the purity was 94% purity, which is likely due to the time elapsed while in transit (It lasts longer if its refrigerated before reconstitution).  Anyway, based on my bodyweight (195 pounds) I do 6IUs 3x a week, along with 100mg DHEA  (im naturally deficient in), 50mg Zinc, 500 mg metformin.  Dr. Fahy believes the metformin is critical.  I just started, but will be testing my levels routinely.  Especially important to check anything related to cancer, including imflamation markers, and PSA levels.   These are likely to improve in the longterm. I will adjust the HGH dosage if I start to get side effects, etc.  

 

I honestly don't know if taking something else that increases IGF-1 will duplicate this.  Dr. Fahy speculates that HGH does more than just increase IGF-1, and it's presence may affect other mechanisms.  Just from what I see from those taking it on forums like eroids.com, HGH seems to provide the most dramatic improvements on things like skin, at least compared to other peptides that affect IGF-1.  And the improvement can be extraordinary.  Check out the latest videos from Dr. Fahy.  He's been on the protocol since January, and his face has really transformed.  He also said a lady in her 70s took it, and within a few months, she was not even recognizable as the same person.  He says it doesn't work on everyone though.  I don't know if Ubiquinol will have this same effect.