https://www.ncbi.nlm...les/PMC6521744/
Involvement of the Endocannabinoid System: In vitro Inhibition of Fatty Acid Amide Hydrolase and Monoacylglycerol Lipase Enzymes and in vivo Pre-treatment With the CB1 Receptor Antagonist AM251
In order to detect the potential implication of the endocannabinoid system in the analgesic activity of LEO, inhibition of the FAAH and MAGL enzymes was tested. LEO showed a dose-dependent response in the enzymatic bioassays (Figure 4A). The reference substance JZL 195 provided by Cayman showed a very similar profile both in the FAAH and MAGL enzymes with an IC50 value between 0.01 and 0.1 μM (Figure 4B). LEO was less potent than the inhibitor but was able to inhibit both enzymes, particularly FAAH (Figure 4A). Pre-treatment with the CB1 receptor antagonist AM251 (4 mg/kg) significantly attenuated LEO-induced anti-allodynic effect (Figure 4C).Inhibition of fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) performed by lavender essential oil (A) and the reference inhibitor JZL 195 (B). © Attenuation of the anti-allodynic effect FIGURE 4of LEO (100 mg/kg p.o.) by the CB1 receptor antagonist AM251 (4 mg/kg i.p.). (One-way ANOVA, F(5,59) = 24.29, p < 0.0001); *p < 0.05, ***p < 0.001 in comparison with CTRL contralateral side; °°°p < 0.001 in comparison with CTRL ipsilateral side.
I didn't want this to get lost in my own thread on amygdala tickling.
But for anyone interested in inhibiting their FAAH and increasing their endocannabinoid levels such as anandamide, look into using lavender oil.
From personal experience, I use the lavender oil pills from a well known brand in the UK called Kalms, which are taken orally/ingested as opposed to inhalation. And I can say without a doubt, and without placebo effect, they are very effective at calming the mind and thoughts.
Another note is lavender also significantly increases theta and alpha waves in all brain regions: https://www.research...trical_Activity
It is felt the effects of lavender inhalation on the brain wave activities are well demonstrated in the present study. During inhalation with lavender, the power of theta (4-8 Hz) and alpha (8-13 Hz) activities are significantly increased in all brain regions.This result is consistent with the study of Diego(8)that found after lavender inhalation that frontal alpha power was significantly increased. Furthermore, a study conducted at the University of Occupational and Environmental Health, Kitakyushu Japan(26)used changes of electroencephalogram (EEG) to measure the effects of aromas. The present study found relaxing effects with increases of alpha wave activities after administering lavender, cineol sandalwood, and alpha-pinene. The EEG evidence of relaxation can be seen in various practices such as meditation. Meditation is a way of balancing the body and the mind as well as controlling the mind to experience feelings of peace and relaxation. The study among people meditating can demonstrate similar EEG changes with lavender inhalation, which presented as an increase in theta and alpha activities in the brain during meditation(27). The increase in theta and alpha activities can also be observed even during pre-meditation states in people who frequently practice meditation(28). These results lend support that increases in theta and alpha wave activity causes a range of general relaxation effects and can be induced by a range of chemical and non chemical techniques.
No idea if this is caused by the FAAH inhibition. But is another interesting thing to note on the possible reasons lavender is mentally calming.
