6 replies to this topic

[Mr Serendipity]

https://www.ncbi.nlm...les/PMC6521744/

 

 

Involvement of the Endocannabinoid System: In vitro Inhibition of Fatty Acid Amide Hydrolase and Monoacylglycerol Lipase Enzymes and in vivo Pre-treatment With the CB1 Receptor Antagonist AM251

 

In order to detect the potential implication of the endocannabinoid system in the analgesic activity of LEO, inhibition of the FAAH and MAGL enzymes was tested. LEO showed a dose-dependent response in the enzymatic bioassays (Figure 4A). The reference substance JZL 195 provided by Cayman showed a very similar profile both in the FAAH and MAGL enzymes with an IC50 value between 0.01 and 0.1 μM (Figure 4B). LEO was less potent than the inhibitor but was able to inhibit both enzymes, particularly FAAH (Figure 4A). Pre-treatment with the CB1 receptor antagonist AM251 (4 mg/kg) significantly attenuated LEO-induced anti-allodynic effect (Figure 4C).

 

Inhibition of fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL) performed by lavender essential oil (A) and the reference inhibitor JZL 195 (B). © Attenuation of the anti-allodynic effect FIGURE 4of LEO (100 mg/kg p.o.) by the CB1 receptor antagonist AM251 (4 mg/kg i.p.). (One-way ANOVA, F(5,59) = 24.29, p < 0.0001); *p < 0.05, ***p < 0.001 in comparison with CTRL contralateral side; °°°p < 0.001 in comparison with CTRL ipsilateral side.

 

 

I didn't want this to get lost in my own thread on amygdala tickling.

 

But for anyone interested in inhibiting their FAAH and increasing their endocannabinoid levels such as anandamide, look into using lavender oil.

 

From personal experience, I use the lavender oil pills from a well known brand in the UK called Kalms, which are taken orally/ingested as opposed to inhalation. And I can say without a doubt, and without placebo effect, they are very effective at calming the mind and thoughts.

 

Another note is lavender also significantly increases theta and alpha waves in all brain regions: https://www.research...trical_Activity

 

 

 

It is felt the effects of lavender inhalation on the brain wave activities are well demonstrated in the present study. During inhalation with lavender, the power of theta (4-8 Hz) and alpha (8-13 Hz) activities are significantly increased in all brain regions.This result is consistent with the study of Diego(8)that found after lavender inhalation that frontal alpha power was significantly increased. Furthermore, a study conducted at the University of Occupational and Environmental Health, Kitakyushu Japan(26)used changes of electroencephalogram (EEG) to measure the effects of aromas. The present study found relaxing effects with increases of alpha wave activities after administering lavender, cineol sandalwood, and alpha-pinene. The EEG evidence of relaxation can be seen in various practices such as meditation. Meditation is a way of balancing the body and the mind as well as controlling the mind to experience feelings of peace and relaxation. The study among people meditating can demonstrate similar EEG changes with lavender inhalation, which presented as an increase in theta and alpha activities in the brain during meditation(27). The increase in theta and alpha activities can also be observed even during pre-meditation states in people who frequently practice meditation(28). These results lend support that increases in theta and alpha wave activity causes a range of general relaxation effects and can be induced by a range of chemical and non chemical techniques.

 

No idea if this is caused by the FAAH inhibition. But is another interesting thing to note on the possible reasons lavender is mentally calming. 

[Mr Serendipity]

BTW I'm no expert on reading studies or anything.

 

But from what I can work out from the graph, lavender oil can inhibit FAAH and MAGL enzymes around 80%. However I have no idea what human dosage for this would be required. I also have no idea what the MAGL enzyme is or what inhibiting it would do, as I haven't looked into it. My main purpose for looking into FAAH inhibitors was to increase my anadamide levels.

[gamesguru]

interesting!  but is there any evidence that FAAH and this approach to endo-cannabinoids really affect the patient or help life quality much?

[Mr Serendipity]

interesting!  but is there any evidence that FAAH and this approach to endo-cannabinoids really affect the patient or help life quality much?

 

Well from my google searches, there's a lot of talk about the possibility of inhibiting FAAH and increasing endo-cannabinoids to help people with anxiety and PTSD issues.

 

I've posted studies which I've found interesting in my amygdala tickling thread but I'm not going to repost them all here. 

 

The only thing I will post is the interesting case of the woman with the FAAH gene mutation and her behaviour:

 

 

A Scottish woman with a rare genetic mutation in her FAAH gene with resultant elevated anandamide levels was reported to be immune to anxiety, unable to experience fear and insensitive to pain. The frequent burns and cuts she suffered due to her hypoalgesia healed quicker than average.^[31][32][33]

 

 

They are big statements:

 

1. immune to anxiety

2. unable to experience fear

3. insensitive to pain

[gamesguru]

so is Salvia^[1].. even tho only at 22% in the amygdala, the authors still speculate the FAAH inhibition contributes to the anxiolytic effect.

 

This was 10 µg·kg⁻¹ in mice and/or rats

[Galaxyshock]

I'll have to get myself some Lavender. In this study it was as effective as Lorazepam for general anxiety: https://pubmed.ncbi....h.gov/19962288/

 

Does it have to be the oil or does the whole herb work as well?

[bullGenteel]

I'm feeling in a bit better state this last week or so. I've figured out some kinks I had with another protocol I was doing. I thought I might add just a little more thoughts about 5htp1a pathway with some cbd2 receptor activation experiences and current tweaks I might make to this.

I did feel like the lavender opened me up to a new way of actually experiencing a lot of feelings for other people. It was like one tier above the functioning of just being interested in socializing more wirh people brought on by l-REUTERI yougart. I thought the heightened interest in socializing was the best I should expect from reuteri. Foe yeh lavender, I remember having suspicions my new found benefits wouldn't be permanent based on the fact I was just giving myself a month to experiment, till I recieved Relora or cbd to trial. I expected to have diminished pro feelings after switching, based of my experience with ginseng as an estorgenic effecting substance. I took ginseng for 8 months or so with also 20g of soy lecithin. I originally suspected lecithin was making me to be over emotional, maybe for first time in my life, even more than my early teen years. I slowly nexxed some of the stack elements responsible for it, when I felt it wasn't quite what normal people feel or wasn't right for me I decided. I had a concussion so I didn't do much complex thinking back then. I don't believe I could think concurrently on 3 subjects at once, if that makes sense. I'm a bit better now, even if I'd always been a bit of a scatterbrained in my thinking style.

I had some of the same pro social feelings, just on Relora but not nearly as strong as lavender which I chalked up to the faah inhibitor what have you, but minus the estrogen potentiated. Maybe I am getting slightly better at researching. I had heard of Berberine and Rhonda Patrick may have stated some negative effects on mitochondria. I had also heard it can be tough on microgut flora, bejng why I wrote it off with myself on reuteri and gut brain axis. It is possible the Relora vs Lavenser that the former was killing down the population of the reuteri. I just discovered that relora has Berberine. Story of my life, the last 4 years being my own orthomolecular consultant. I took Relora for about a year.

I've been doing another protocol, so I only experimented a little with replacing relora soley with magnolia bark extract. Well first, I added NAG to relora, before I read about the Berberine inclusion thinking NAG might effect the 5ht1a receptor. I haven't put much effort into studying or note taking and my memory is bad. I thought that the NAG was an adequate replacement for CBD, maybe mild confabulation or just badly confused memory. I am slow at researching I may have been wrong, I don't know if maybe, just Marijuana has some effect on telomeres but not CBD.

For NAG, it maybe that it has chitin, which I guess is a prebiotic so maybe it aids the colonizing of the reuteri. I did take NAG when I first started taking reteuri about 3 years ago and it felt like night and day difference almost immediately. It wasn't just placebo I think it changed me. Not to sound overly dramatic. I probably have the bio-pathological symptoms that respond positively to it.

I did read that chitin, but perhaps mostly chitosan can encapsulate or create polymers, or what not, that enhance the cellular absorption of hormones, one study said, in addtion to increased absorption of elements such as reversatrol, curcumin and quercetin, I believe. But chitosan is glucosamine coupled with NAG thru acetylization, it doesn't happen when the individual elements are ingested. I know some people, admittedly far more knowledgeable than me, have used mixtures of lecithin with some elements to boost absorption of such elements, in stay of lipsomal formulas. Fruit juice is sometimes used with study references. There is likely better reasoning they have than myself, whose just guesstimate if chitin in NAG can increased absorption or enhance oxytocin or other pathways like the faah inhibition or cabbinoid #2 receptor. It likely just acts as a prebiotic.

I like to think I have worked in myself with personal growth. I believe reuteri was absolutely necessary for myself as well as addition of cabbinoid recptor or faah inhibitor activity also is very helpful to me.

I am also attempting to treat a moderate concussion amount other things. I will see how the dropped Relora and addition of magnolia bark combined with 250mg of NAG works, along side the reuteri yougart.