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P A Y W A L L E D S O U R C E : Mechanisms of Ageing and Development
Highlights
• Fucoxanthin, a main marine carotenoid, can cause antioxidant effect in the late passage human cells.
• Transcriptomic data showed increasing expression level of genes related to the Nrf2/ARE pathway.
• Fucoxanthin altered cellular processes, including ribosome biogenesis, lipid metabolism, cell cycle regulation and age-related pathways such as Wnt, JAK-STAT and FoxO signaling pathways.
Abstract
Fucoxanthin, as a main marine carotenoid, exhibit a wide variety of bioactivities, including antioxidant activity. Previously, we have shown the geroprotective activity of fucoxanthin on Drosophila and C. elegans.
Our new study aimed to compare the antioxidant activity of fucoxanthin in early and late passage normal human cells LECh4(81) in physiological conditions and under oxidative stress. In addition, using the RNA-seq we have analyzed the transcriptomic changes during the replicative senescence of fibroblasts treated with fucoxanthin.
Results showed that fucoxanthin at a concentration of 5 μM caused the most pronounced antioxidant effect in the late passage cells. Moreover, transcriptomic data showed the increased expression levels of genes related to the Nrf2/ARE pathway.
According to the analysis of enriched KEGG pathways, fucoxanthin altered cellular processes like ribosome biogenesis, lipid metabolism, and cell cycle regulation including some age-related pathways such as Wnt, JAK-STAT, and FoxO signaling pathways. We suggest that fucoxanthin may have therapeutic potential for treating age-related diseases.
Abbreviations
KEGG_Kyoto Encyclopedia of Genes and Genomes
DE_differential expression
ROS_reactive oxygen species
MMP_mitochondrial membrane potential
SASP_Senescence-associated secretory phenotype
OUTLINE:
1. Introduction
2. Materials and Methods
2.1. Cell culture
2.2. Viability assay
2.3. Senescence-associated β-galactosidase (SA- β-gal) activity
2.4. Detection of ROS and MMP levels
2.5. RNA isolation and sequencing of mRNA
2.6. Bioinformatics analysis
3. Results
3.1. The influence of fucoxanthin concentration on the viability of human embryonic lung fibroblasts
3.2. The influence of fucoxanthin on SA- β-gal activity
3.3. Fucoxanthin influence on antioxidant activity in normal human embryonic lung fibroblasts
3.4. Fucoxanthin influences on mitochondrial membrane potential
3.5. Transcriptome analysis
4. Discussion
Funding
Data availability
Declaration of Competing Interest
Acknowledgments
Appendix A. Supplementary data
References
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Edited by Engadin, 25 May 2020 - 08:18 PM.
