Its quite difficult to assess. I have moved the H2 to 20 mins each morning. However, I don't have a lot of identified health issues to resolve. My theory of the H2 is that 20 mins will to some extent saturate the body and act to reduce particular the Hydroxyl radical from causing oxidative stress for a good few hours. However, that can only really be measured in a macro measurement.
What I can say is that I am not aware of any negative side effects.
It does get quite hard with quite a wide ranging protocol (which in my view is the correct approach) to isolate out the effects of any part of that protocol.
A lot of the things I do are in esssence targeted on the mitochondria. I have moved the O2 to a good few hours away from H2 and Melatonin. That is because Melatonin tends to switch off HIF and O2 is looking for a shift in ROS to enable HIF. Hence if I go for upregulating HIF whilst serum Melatonin is high and ROS are low that is not clever. Going for O2 then H2 would perhaps be better, but I like doing the gas therapies early in the morning whilst I am typing things up and doing my morning routine (I am using the H2 at the moment).
One hypothesis I have about HIF is that it comes on when mitochondrial Melatonin goes low and mitochondrial Melatonin goes low when there are a lot of ROS to neutralise. Taking out mitochondrial Melatonin itself, however, does not stop the antioxidant process as Melatonin is the first anti-oxidant in a sequence of molecules which may allow the reduction of up to 10 Oxidants.
I have had a further thought on this. This approach would work particularly well if the production of mitochondrial Melatonin was in some way linked to the level of oxygenation (the partial pressure of Oxygen). To some extent, of course, that is true although it also links to the availability of glucose.
Mitochondrial Melatonin requires as a substrate Acetyl-CoA which the Mitochondria get from Oxidative Glycolysis. So if you switch down the Oxygen you produce less Melatonin hence that which is already there gets depleted for clearing up the ROS. I have no idea how the cells would determine what proportion of Acetyl-CoA goes into the Krebs Cycle and what propoportion is used for Melatonin production and what proportion wanders out of the cells in search of the Nucleus for acetylation etc etc. (and what other things might happen).
However, that would explain the response from HIF to an increase In ROS which is not associated with an increase in the partial pressure of Oxygen and would also explain why a reduction in the partial pressure of Oxygen would cause HIF to switch on.
What I would say about the machine, however, is that I am very happy with the machine itself and the support that was available although I set it up quite easily myself. (with reading their online documentation).
Edited by johnhemming, 13 February 2022 - 09:15 AM.