2 replies to this topic

[Believer]

hTERT supposedly does not enter into cells from the blood stream so injecting it would not do anything.

However several fragments and mutants of hTERT possess different properties

 

This is one hTERT fragment that does enter into cells

https://pubmed.ncbi....h.gov/29568955/

 

Why are we not having fragments discussed in literature synthesized in a group buy and tested?

[QuestforLife]

I've always wondered why we can't just inject recombinant telomerase. If telomerase can't enter cells from outside, then how do feeder cells work? Generally feeder cells are irradiated mouse embryonic stem cells that secrete growth factors including telomerase, which cause the cell culture to continue dividing.

 

The question for this fragment is does it lengthen telomeres; not all do? Having scanned the paper, it appears the answer is yes, although it isn't tested. 

 

Of note, GV1001 treatment markedly elevated the level of GRHL2, a transcription factor with diverse biological functions and a multitude of target genes, including hTERT, proliferating cell nuclear antigen, p63, microRNA-200 family genes and epidermal cell differentiation complex genes (24–26,36). GRHL2 promotes epithelial cell proliferation in part through the transcription regulation of hTERT; the ectopic overexpression of GRHL2 in primary NHOKs led to marked extension of cell life span with continued expression of hTERT and telomerase activity (25). As GV1001 is derived from the hTERT protein sequence, the fact that GV1001 enhanced GRHL2 may indicate a feed-forward mechanism by which it further elevates the level of hTERT through the expression of GRHL2

[Believer]

I've always wondered why we can't just inject recombinant telomerase. If telomerase can't enter cells from outside, then how do feeder cells work? Generally feeder cells are irradiated mouse embryonic stem cells that secrete growth factors including telomerase, which cause the cell culture to continue dividing.

 

The question for this fragment is does it lengthen telomeres; not all do? Having scanned the paper, it appears the answer is yes, although it isn't tested. 

I am confused, it seems that GV1001 both has studies saying that it reduces telomere elongation while other studies say the opposite? it was developed for treating cancer.

Needless to say this is just one out of many possible hTERT fragments