Metyhelene is so underdosed usually, so much that it won't cause any effect regarding MAO-A inhibition other than placebo. 300 mg (or more) of methylene blue increases the concentration of norepinephrine and serotonin much more than 15 or 30 mg. This is because despite 15 mg inhibiting 80% of mao-a, being reversible inhibitors they compete with serotonin (e.g.) and eventually the concentration of it increases until it wins against methylene blue and therefore begins to be degraded. Consequently, more reversible inhibitor dosage = more effectiveness, even though complete inhibition is achieved at lower doses! The opposite is true for the irreversible ones. You can use affinity values to calculate everything mathematically (which in fact is measured in moles of the substance).
This, in addition to being considered the norm by this basic theory, I have tried it on myself and I can assure you that 200 mg of methylene blue has a REALLY powerful antidepressant effect, as opposed to 30 mg, even if it is still noticeable.
I specify it with a post because if a person without special knowledge does a research on the recommended dose for an antidepressant effect, everywhere, it is said that a dose of 15 mg, inhibiting 80% of mao-a in normal conditions, is the optimal one, in reality it is an extremely low dose considering the competition with the ligands. I saw people who tried sub 15 mg doses for depression and noticing some sort of effects: it is just placebo.
If you are Italian I wrote about this and much more in my telegram channel @sciemenza.
Edited by Frank Walter, 26 March 2022 - 07:15 AM.
