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Two Studies Suggesting C60-induced DNA damage in rats?

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#1 katzenjammer

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Posted 30 December 2022 - 04:45 PM


These two studies--at least on the surface=--are concerning.  On the other hand, they've been around for a while and may have already been beaten to death?  

 

1.  C60 oil caused DNA damage in rats, changing the DNA expression of the lungs and liver.   "Oxidatively Damaged DNA in Rats Exposed by Oral Gavage to C60 Fullerenes and Single-Walled Carbon Nanotubes," 2009.  

 

 

2.  C60 induced sister chromatid exchange and micronucleation, which can be a source of DNA mutation.  "In Vitro and in Vivo Genotoxicity Induced by Fullerene (C60) and Kaolin," 2011.  
 
 
 


#2 abelard lindsay

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Posted 31 December 2022 - 02:15 AM

If rats live twice as long when they take C60, does any of this matter? 


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Click HERE to rent this advertising spot for C60 HEALTH to support Longecity (this will replace the google ad above).

#3 katzenjammer

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Posted 31 December 2022 - 01:35 PM

If rats live twice as long when they take C60, does any of this matter? 

 

The answer is "yes."  

 

We might learn from rat experiments -- and take the results seriously - but we aren't rats most of us.  It's not 1 to 1.  


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#4 Mind

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Posted 31 December 2022 - 02:21 PM

If rats live twice as long when they take C60, does any of this matter? 

 

The original study was tiny (n=6) and has never been replicated. Nor has it been conclusively shown whether or not the rats lived longer solely because of dietary changes or olive oil consumption.


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#5 Rocket

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Posted 02 January 2023 - 01:50 AM

The original study was tiny (n=6) and has never been replicated. Nor has it been conclusively shown whether or not the rats lived longer solely because of dietary changes or olive oil consumption.


I don't take what i read on the internet as gospel truth. There is a huge effort by world governments to suppress medical breakthroughs and keep people sick. If something like c60 was found to benefit health there would be a huge effort to suppress it. Its been done before. Its being done now with covid. 10 years ago you could buy piracatem anywhere and now you practically have to go to the dark web. I've had great results with c60 and a few conditions its greatly aided.

Edited by Rocket, 02 January 2023 - 01:53 AM.

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#6 katzenjammer

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Posted 02 January 2023 - 02:12 AM

I don't take what i read on the internet as gospel truth. There is a huge effort by world governments to suppress medical breakthroughs and keep people sick. If something like c60 was found to benefit health there would be a huge effort to suppress it. Its been done before. Its being done now with covid. 10 years ago you could buy piracatem anywhere and now you practically have to go to the dark web. I've had great results with c60 and a few conditions its greatly aided.

 

I agree with the basic substance of what you're saying.  

 

On the other hand, if you're going to reject [xxx} - pushed by [xxx] - we also want to be as evidenced based as possible ourselves, no?  

 

So if [xxx] wants to inject me with [xxx] - not demonstrated to be necessary, safe or effective - I'm going to be careful to include in my protocols only what I believe - based on substantiated evidence - to be necessary, safe and effective.

 

The problem with C60 as I see it is that while it does seem to offer short term benefits, I question whether we really understand the long term risks.  However, I am just asking questions, which is an important thing to do.  

 

Just because it has "aided" in some/all of your own conditions doesn't mean by itself that it won't cause some other condition.  

 

Now you might say: "these conditions of mine are so horrible that to me it's worth that risk."  And believe me when I say that I respect that.  

 

But that's a far cry from saying that everyone  - no matter how healthy, whatever age - should start taking C60.  And if you read the SES Res. marketing pitches, it would be easy to think that everyone should.  


Edited by katzenjammer, 02 January 2023 - 02:13 AM.

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#7 Hip

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Posted 02 January 2023 - 05:21 AM

There is a huge effort by world governments to suppress medical breakthroughs and keep people sick. If something like c60 was found to benefit health there would be a huge effort to suppress it. 

 

You don't have to be gullible to believe in conspiracy theories; but it certainly helps.


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#8 Anthony_Loera

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Posted 04 January 2023 - 12:22 AM

But how many conspiracy theories have come true?
(me reading about the latest CIA / Kennedy one... :unsure: )
 
Now Back To The Topic:
Fathi mentioned he has done many studies on C60 previously, and if you search you will find that this was not his first.
In March of 2022 he did write an article concerned, not because of the C60 Olive Oil, but of fake or bad quality c60.
 
The text is below from this link:
https://www.research...rolong_lifespan
 
Can [60]fullerene maintain good health and prolong lifespan?
By Fathi Moussa
 
Fatalists aside, who doesn’t want to live longer and in good health? But, obviously, to live longer it would be nonsense to put our health at risk. Yet, since we published a paper in 2012 on prolonging the lifespan of rats by repeated administration of [60] fullerene (C60), myriad websites have started selling this product online as a dietary supplement without any certified toxicity test! Safety precautions  Since the early nineties countless publications have reported promising medicinal applications for C60 mostly related to its unparalleled affinity towards free radicals. However, until now no officially approved C60-based drug has reached the market, notably because of the alleged dangers of C60 nanoparticles.
 
 
post-4527-0-42502400-1672790605.png
 
 
From our experience in the field of biomedical applications of fullerenes, we know that pure C60 has neither acute nor chronic toxicity. Nevertheless, we also know that under certain conditions, linked to the presence of impurities into C60 aggregates or to defective adjuvants or to light exposure, certain preparations based on this product, can be highly toxic. Hence, it is important above all to avoid using C60 in solid form, i.e. in the form of aggregates or nanoparticles. Especially since, we should remember, C60 is active only in soluble form.
 
Overall, it is mandatory to verify the innocuousness of any C60-containing preparation proposed for human consumption. Is C60 active in all species regardless of age?  Recently, two studies found no beneficial effect of C60 administration on aging in mice. The authors therefore questioned the generalizability of the efficacy data across different species and age ranges. Meanwhile, many consumers testify online about the benefits of C60 on their health. But, of course, these testimonies, whether solicited or not by the sellers, cannot be acceptedf only for the omission of the placebo effect.
 
While pharmacokinetics data are missing in both studies in mice – i.e. we do not know if the administered ingredient is well absorbed and if so to what extent? -, their results clearly remind us, if needed, that clinical trials are unavoidable before proposing any C60 preparation for human consumption. Towards Regulatory Considerations  In order to perform successful clinical trials, the purity of an active pharmaceutical ingredient is a crucial prerequisite. This should also apply to C60, even though it is only sold as a dietary supplement.
 
In order to build a quality-testing strategy for regulatory considerations, we have recently evaluated several analytical tools to verify the purity of commercially available C60 samples. Our data clearly show that differential scanning calorimetry is the best choice to establish a purity criterion based on the sc-fcc transition of a C60 sample (Tonset ≥ 258 K, Dsc-fccH ≥ 8 J/g). Nevertheless, even the visual aspect of a C60 sample can already provide clues as to its quality. Highly pure C60 samples are crystalline and exhibit a black metallic appearance (Fig. 1a), whilst less pure C60 samples are less crystalline, and possess a brownish color (Fig. 1b, and c).
 
Furthermore, oily solutions of C60 are clear and have a purplish orange rose color, the intensity of which depends on the concentration (Fig. 1). This color results from mixing the yellow color of the oil and the violet color of C60 in solution. Any cloudy or differently colored solution should be discarded. As there are neither regulatory recommendations on this subject nor a purity criterion for C60 samples until now, C60 consumption could cause a public health issue. This would be doubly damaging. First of all, for consumers’ health of course, but also this could permanently compromise a product which is so promising for human health.
 
 
Fathi Moussa
Institut de Chimie Physique, CNRS – UMR 8000, Université Paris – Saclay, France
Attached File  c60 olive oill by Fathi Moussa x- March 2022.png   349.61KB   0 downloads

 

 

Now, do I believe in the findings that this seasoned researcher has provided? Yes

How much? Today is my third day taking 183.26 ml of C60 Olive Oil that I made myself.

 

I have 4 more days to go...

and I am not dead yet (love Monty Python)  :)

 


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#9 katzenjammer

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Posted 04 January 2023 - 01:36 AM

 

Now, do I believe in the findings that this seasoned researcher has provided? Yes

How much? Today is my third day taking 183.26 ml of C60 Olive Oil that I made myself.

 

I have 4 more days to go...

and I am not dead yet (love Monty Python)  :)

 

Glad you're not dead yet.  haha!  But the real question is:  is that african or european olive oil?  

 

183ml?  Wow, that is a lot.

 

I get why you think it has at least short-term benefits; what makes you think it's safe?  


Edited by katzenjammer, 04 January 2023 - 01:36 AM.


#10 Anthony_Loera

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Posted 04 January 2023 - 04:45 PM

Why do I think its safe?

 

I purchased 99.9% C60 that was baked to burn off all the residues...

(you are not guaranteed this if you buy it already made)

 

and used organic Olive Oil from a source who sells it by the bucket...

 

Then made the C60oo myself. 

no one else was involved.

 

A



#11 katzenjammer

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Posted 04 January 2023 - 07:38 PM

Why do I think its safe?

 

I purchased 99.9% C60 that was baked to burn off all the residues...

(you are not guaranteed this if you buy it already made)

 

and used organic Olive Oil from a source who sells it by the bucket...

 

Then made the C60oo myself. 

no one else was involved.

 

A

 

Yes, but just because you prepared, say, a strychnine liquor yourself (yummmm!) -- even if no one else was involved -- doesn't make it safe to ingest.  

 

Let's assume the C60 anyone is using is very pure.  

 

And let's assume that there are in fact at least short term health benefits. 

 

How do you know that there aren't long term risks?  

 

What risks?  Such as those implied by the above two studies? 


Edited by katzenjammer, 04 January 2023 - 07:38 PM.

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#12 Rocket

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Posted 05 January 2023 - 01:38 AM

Keep us posted in your research

#13 joesixpack

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Posted 06 January 2023 - 12:03 AM

The two studies mentioned in the original post mixed C60 with other things. Carbon nanotubes, which are toxic, and Kaolin, which is mentioned to be a possible cause of the dna damage found in the study.

 

Here is a short thread from 2012 that you might find of interest.

 

https://www.longecit...-c60-was-toxic/


Edited by joesixpack, 06 January 2023 - 12:04 AM.

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#14 katzenjammer

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Posted 06 January 2023 - 02:46 PM

The two studies mentioned in the original post mixed C60 with other things. Carbon nanotubes, which are toxic, and Kaolin, which is mentioned to be a possible cause of the dna damage found in the study.

 

Here is a short thread from 2012 that you might find of interest.

 

https://www.longecit...-c60-was-toxic/

 

Many thanks, I suspected these had been discussed before, given how long they've been around.  



#15 gamesguru

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Posted 18 June 2023 - 01:59 PM

I am not sure if adverse effects are limited to non-pristine C60, caution is warranted at higher doses.

 

Acute exposure to C60 fullerene damages pulmonary mitochondrial function and mechanics
2021 Apr
https://pubmed.ncbi....h.gov/33370539/

C60 fullerene (C60) nanoparticles, a nanomaterial widely used in technology, can offer risks to humans, overcome biological barriers, and deposit onto the lungs. However, data on its putative pulmonary burden are scanty. Recently, the C60 interaction with mitochondria has been described in vitro and in vivo. We hypothesized that C60 impairs lung mechanics and mitochondrial function.

Lung tissue elastance and viscance were augmented in FUL group. Increased inflammatory cell number, alveolar collapse, septal thickening, and pulmonary edema were detected. In other six FUL and six CTRL mice, mitochondria expressed reduction in state 1 respiration, ATP production, and higher oxygen consumption in state 4, generation of reactive oxygen species and reason ROS/ATP. In conclusion, exposure to fullerene C60 impaired pulmonary mechanics and mitochondrial function, increased ROS concentration, and decrease ATP production.


Reconciling the controversial data on the effects of C60 fullerene at the organismal and molecular levels using as a model Drosophila melanogaster
August 2019
https://www.scienced...378427418319143

The results of such studies range from direct destabilizing effects on DNA molecules to amelioration of the toxic effects of known genotoxic agents. We tested the effect of buckminsterfullerene (C60) on Drosophila melanogaster at DNA, tissue and organism levels.

The water-soluble pristine C60 fullerene at the concentration of 20 μg/ml and 40 μg/ml leads to the activation of the mus209 gene in D. melanogaster larvae salivary glands, which can indicate higher levels of DNA damage. However, the absence of effects at the cell and organismal level could be explained by the activation of repair systems or by active elimination of damaged cells.


C60 in olive oil causes light-dependent toxicity and does not extend lifespan in mice
29 October 2020
https://link.springe...357-020-00292-z

We additionally find that pristine C60-OO causes no acute toxicity in a rodent model but does form toxic species that can cause significant morbidity and mortality in mice in under 2 weeks when exposed to light levels consistent with ambient light.

We failed to observe significant lifespan and health span benefits of C60-EVOO or EVOO supplementation compared to untreated controls, both starting the treatment in adult or old age. Our results call into question the biological benefit of C60-OO in aging.


Cytogenotoxic effects of fullerene C60 in the freshwater teleostean fish, Anabas testudineus
November 2019
https://www.scienced...383571819302141

C60-DMSO suspension at sublethal concentrations (5 and 10 mg/L) showed cytogenotoxicity in the freshwater fish, Anabas testudinues.

DNA-damaging effect of fullerene C60 nanomaterial was evident by a significant increase in the micronucleus frequency and percent tail DNA in erythrocytes, gill and liver cells of the freshwater fish, Anabas testudineus.

Cytotoxicity of fullerene C60 was evident by the formation of sticky cells, vacuolated cytoplasm, cytoplasmic degeneration, echinocyte, acanthocyte, anisochromatic cells and abnormal erythrocyte membrane.

DNA damages scored from gill and liver cells showed different sensitivities, and the overall degree of genotoxicity observed were at high grades in gill cells followed by liver and erythrocytes of the fish, Anabas testudineus.


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