1084 replies to this topic

[sUper GeNius]

I asked whether anyone thought curcumin would help t-res absorption. See

http://en.wikipedia.org/wiki/Curcumin

"Curcuminoids induce glutathione S-transferase and are potent inhibitors of cytochrome P450."

Comments?

[maxwatt]

I rather like this recipe:

-Dissolve 3 grams of resveratrol in 30 ml of 80 proof vodka, set aside. (or use 90% ethanol, adjust proportions)
-take a heaping tsp of lecithin and add to one cup of water; mix until dissolved. Use of a blender speeds the process. I use a Braun mini-primer with mixing jar and blade. Any mini-blender should be good.
-Strain the lecithinated water to remove any lumps of lecithin.
-Add the resveratrol-vodka mix to the water and stir.

Maxwatt, can you really get 3 grams into 30ml of 80 proof vodka (40% EtOH)? I use Everclear (95% EtOH), and I don't think I can get 3 grams into 30ml of that. Maybe 2 grams, or a little more, but it's more than twice as concentrated in EtOH... I've never been brave enough to microwave it, and water baths are too much hassle, so I haven't tried heating it.

I stirred the bejesus out of it; the powder was extremely fine, which helped. It was not clear, so may have been a suspension with very small particle size.

[niner]

I asked whether anyone thought curcumin would help t-res absorption. See

http://en.wikipedia.org/wiki/Curcumin

"Curcuminoids induce glutathione S-transferase and are potent inhibitors of cytochrome P450."

Comments?

I hate seeing things that say "potent inhibitors of cytochrome P450" as though there were only one P450. There are lots of them, with very different specificity. That said, I don't think that any P450s play a significant role in resveratrol metabolism. It's already pretty well oxidized, so it goes straight to phase II metabolism where it gets conjugated with sulfate and glucuronidate. I don't think glutathione S-transferase is involved either, so I'd have to say that on the basis of the above, curcumin probably isn't going to have much effect on resveratrol bioavailability. Resveratrol is already absorbed pretty well. It's just conjugated so efficiently that its bioavailability is therefore quite low.

[sUper GeNius]

I asked whether anyone thought curcumin would help t-res absorption. See

http://en.wikipedia.org/wiki/Curcumin

"Curcuminoids induce glutathione S-transferase and are potent inhibitors of cytochrome P450."

Comments?

I hate seeing things that say "potent inhibitors of cytochrome P450" as though there were only one P450. There are lots of them, with very different specificity. That said, I don't think that any P450s play a significant role in resveratrol metabolism. It's already pretty well oxidized, so it goes straight to phase II metabolism where it gets conjugated with sulfate and glucuronidate. I don't think glutathione S-transferase is involved either, so I'd have to say that on the basis of the above, curcumin probably isn't going to have much effect on resveratrol bioavailability. Resveratrol is already absorbed pretty well. It's just conjugated so efficiently that its bioavailability is therefore quite low.

This is written a little further down in the article:

Little curcumin, when eaten, is absorbed — 2 grams of curcumin alone resulted in undetectable to very low serum levels.[13] Much of it is excreted through glucuronidation. Co-supplementation with 20 mg of piperine (extracted from black pepper) significantly increased the absorption of curcumin by 2000%

[zawy]

Or a few of us (including me and you, since it's our idea) could create an ImmInst resveratrol energy drink and then we could retire--call it ImmErgy, ImmPact, ImmErse, ImmRes or something like that...

No, it has to be a spiffy or clear name. Like "Revgenitry" or have a blueberry wine with "Super-Resveratrol" right below the "Blueberry". And "shake well". Any health nut would have to try it. The resveratrol would need to be prominent or any label. "100 times the resveratrol found in wine."

[luminous]

I asked whether anyone thought curcumin would help t-res absorption. See

http://en.wikipedia.org/wiki/Curcumin

"Curcuminoids induce glutathione S-transferase and are potent inhibitors of cytochrome P450."

Comments?

I hate seeing things that say "potent inhibitors of cytochrome P450" as though there were only one P450. There are lots of them, with very different specificity. That said, I don't think that any P450s play a significant role in resveratrol metabolism. It's already pretty well oxidized, so it goes straight to phase II metabolism where it gets conjugated with sulfate and glucuronidate. I don't think glutathione S-transferase is involved either, so I'd have to say that on the basis of the above, curcumin probably isn't going to have much effect on resveratrol bioavailability. Resveratrol is already absorbed pretty well. It's just conjugated so efficiently that its bioavailability is therefore quite low.

This is written a little further down in the article:

Little curcumin, when eaten, is absorbed — 2 grams of curcumin alone resulted in undetectable to very low serum levels.[13] Much of it is excreted through glucuronidation. Co-supplementation with 20 mg of piperine (extracted from black pepper) significantly increased the absorption of curcumin by 2000%

Glad you repointed that out, Fm! I've taken curcumin regularly for a couple of years and never wondered about bioavailability. Now I see that virtually none of it ever "took"--no wonder I'm still so forgetful... Know of a cheap, quality source of piperine? Maybe I should just do it the old-fashioned way by making tasty curry dishes which include pepper.

This thread about resveratrol's bioavailability has gotten me thinking about everything else I take. I wonder how many of my other supplements are not getting through? Must do more research....

[sUper GeNius]

I asked whether anyone thought curcumin would help t-res absorption. See

http://en.wikipedia.org/wiki/Curcumin

"Curcuminoids induce glutathione S-transferase and are potent inhibitors of cytochrome P450."

Comments?

I hate seeing things that say "potent inhibitors of cytochrome P450" as though there were only one P450. There are lots of them, with very different specificity. That said, I don't think that any P450s play a significant role in resveratrol metabolism. It's already pretty well oxidized, so it goes straight to phase II metabolism where it gets conjugated with sulfate and glucuronidate. I don't think glutathione S-transferase is involved either, so I'd have to say that on the basis of the above, curcumin probably isn't going to have much effect on resveratrol bioavailability. Resveratrol is already absorbed pretty well. It's just conjugated so efficiently that its bioavailability is therefore quite low.

This is written a little further down in the article:

Little curcumin, when eaten, is absorbed — 2 grams of curcumin alone resulted in undetectable to very low serum levels.[13] Much of it is excreted through glucuronidation. Co-supplementation with 20 mg of piperine (extracted from black pepper) significantly increased the absorption of curcumin by 2000%

Glad you repointed that out, Fm! I've taken curcumin regularly for a couple of years and never wondered about bioavailability. Now I see that virtually none of it ever "took"--no wonder I'm still so forgetful... Know of a cheap, quality source of piperine? Maybe I should just do it the old-fashioned way by making tasty curry dishes which include pepper.

This thread about resveratrol's bioavailability has gotten me thinking about everything else I take. I wonder how many of my other supplements are not getting through? Must do more research....

I use Vitacost curcumin with piperine. I do think though you have been absorbing some, maybe enough. Read towards the bottom here:
http://www.accelerat...s/curcumin.html

I take curcumin for a different reason. Seems that it stops certain MS like symptoms in its tracks. Not quite sure if it's MS, but I find that it works. It might be simply an inner ear issue. I think it is likely though to be some demylenating issue.

[missminni]

Somebody needs to put 500 mg in a caffiene-free energy/mood drink. If you can't figure out a soluble formulation, then work out a contract with a small winery. I know of a muscadine/blueberry winery that has grown pretty good. Still small enough to listen, act fast (i.e. change label), and capable of a good gentleman's agreement for fair payment. Lay the ground work now, then wait for the studies to come in. You could have a year jaw-dropping profits before someone big comes in. One of the big hits in the past year was a stupid little vitamin drink, and you know how big energy drinks got in the past 5 years. This is combining the two. Only those of us reading this know how strong the energy factor is for the first few days. Know the future, catch the wave, retire. Knowing the future is so much better than working.

Great idea. Fifty cent made a kazillion dollars with a stupid little vitamin drink.
All you need to do is partner with a rapper.

Or a few of us (including me and you, since it's our idea) could create an ImmInst resveratrol energy drink and then we could retire--call it ImmErgy, ImmPact, ImmErse, ImmRes or something like that...

You can call it anything you want, but if you want to make enough money to retire for life, get a rapper to endorse it.

Edited by missminni, 20 January 2008 - 07:02 PM.

[stephen_b]

I've followed this recipe, except dissolving 1 g into about 10 ml. It looks like it dissolves to me. I don't bother with straining the lumps of the soy lecithin, though. Not too different in texture from custard, and not unpleasant. In fact, I find a glass of water with lecithin to be quite refreshing.

Stephen

[maxwatt]

I've followed this recipe, except dissolving 1 g into about 10 ml. It looks like it dissolves to me. I don't bother with straining the lumps of the soy lecithin, though. Not too different in texture from custard, and not unpleasant. In fact, I find a glass of water with lecithin to be quite refreshing.

Stephen

Maybe if I perceived the undissolved lecithin as custard instead of lumps of snot it would work for me.

[rhc124]

My 77 year old mother had knee replacement surgery about three months ago. She still has a lot of pain and swelling. Do you guys think that a DMSO/ Resveratrol solution would help her condition. If so, could I mix the resv into some DMSO gel for her to apply over time. How long do you think Trans Resv would last in such a mixture. Thanks

[missminni]

My 77 year old mother had knee replacement surgery about three months ago. She still has a lot of pain and swelling. Do you guys think that a DMSO/ Resveratrol solution would help her condition. If so, could I mix the resv into some DMSO gel for her to apply over time. How long do you think Trans Resv would last in such a mixture. Thanks

Don't use the gel. I was told by Dr. Jacobs that the gel and cream were not as good a transport as the oil.
The spray that is 70%DMSO and 30%Aloe, or 30% water is an excellent transport. Spray DMSO in the palm of your hand, add a little resveratrol powder and rub it on the knee.
I wish I knew about it when my dad had his hip replacement.
As far as premixing, I think that was discussed before but I am not sure what the decision was. Somebody here will know.
Good luck

Edited by missminni, 21 January 2008 - 09:04 PM.

[luminous]

My 77 year old mother had knee replacement surgery about three months ago. She still has a lot of pain and swelling. Do you guys think that a DMSO/ Resveratrol solution would help her condition. If so, could I mix the resv into some DMSO gel for her to apply over time. How long do you think Trans Resv would last in such a mixture. Thanks

Don't use the gel. I was told by Dr. Jacobs that the gel and cream were not as good a transport as the oil.
The spray that is 70%DMSO and 30%Aloe, or 30% water is an excellent transport. Spray DMSO in the palm of your hand, add a little resveratrol powder and rub it on the knee.
I wish I knew about it when my dad had his hip replacement.
As far as premixing, I think that was discussed before but I am not sure what the decision was. Somebody here will know.
Good luck

Thanks, missminni--I just went ahead and ordered your recommended DMSO. At the same site, I found and ordered some LIQUID lecithin (http://www.herbalrem...hin-liquid.html)--so I can avoid the uh, custard consistency. Agreed about your excellent suggestion to ensure we can retire for life. I'll do the difficult legwork of coming up with a clever name. You can have the easy task of finding the world-famous rapper. Let me know...

[missminni]

Thanks, missminni--I just went ahead and ordered your recommended DMSO. At the same site, I found and ordered some LIQUID lecithin (http://www.herbalrem...hin-liquid.html)--so I can avoid the uh, custard consistency. Agreed about your excellent suggestion to ensure we can retire for life. I'll do the difficult legwork of coming up with a clever name. You can have the easy task of finding the world-famous rapper. Let me know...

LOL
okay, I'll start doing my research.

[sUper GeNius]

Anyone think it not wise to take a green tea supplement? I seem to remember that someone said it might inhibit Sirt.

[rhc124]

Anyong else tried the premixing of seven days worth of Revs, everclear, lecithin and water? My batch by the 4th day started getting pretty nasty and on the 5th day gave me an upset stomach.

[maxwatt]

Anyone think it not wise to take a green tea supplement? I seem to remember that someone said it might inhibit Sirt.

The paper on stability showed that under oxidizing conditions, that it did not activate Sirt1. This was in vitro; in vivo, oxygen levels are around 5%intracellularly, much lower. Using antioxidants stabilized it so it did activate Sirt1. A little vitamin C with your green tea, sir? I do not think it is really a problem. I drink a lot of green and white tea.

Edited by maxwatt, 26 January 2008 - 01:58 AM.

[health_nutty]

Anyone think it not wise to take a green tea supplement? I seem to remember that someone said it might inhibit Sirt.

The paper on stability showed that under oxidizing conditions, that it did not activate Sirt1. This was in vitro; in vivo, oxygen levels are around 5%intracellularly, much lower. Using antioxidants stabilized it so it did activate Sirt1. A little vitamin C with your green tea, sir? I do not tink it is really a problem. I drink a lot of green and white tea.

Listen to Maxwatt, His big toe does not lie!

[sUper GeNius]

Anyone think it not wise to take a green tea supplement? I seem to remember that someone said it might inhibit Sirt.

The paper on stability showed that under oxidizing conditions, that it did not activate Sirt1. This was in vitro; in vivo, oxygen levels are around 5%intracellularly, much lower. Using antioxidants stabilized it so it did activate Sirt1. A little vitamin C with your green tea, sir? I do not tink it is really a problem. I drink a lot of green and white tea.

Listen to Maxwatt, His big toe does not lie!

We ought to give the little guy a name. How about "Minwatt?"


Maxwatt: Thanks for the green tea info.

[bentl]

I rather like this recipe:

-Dissolve 3 grams of resveratrol in 30 ml of 80 proof vodka, set aside. (or use 90% ethanol, adjust proportions)
-take a heaping tsp of lecithin and add to one cup of water; mix until dissolved. Use of a blender speeds the process. I use a Braun mini-primer with mixing jar and blade. Any mini-blender should be good.
-Strain the lecithinated water to remove any lumps of lecithin.
-Add the resveratrol-vodka mix to the water and stir.

Maxwatt, can you really get 3 grams into 30ml of 80 proof vodka (40% EtOH)? I use Everclear (95% EtOH), and I don't think I can get 3 grams into 30ml of that. Maybe 2 grams, or a little more, but it's more than twice as concentrated in EtOH... I've never been brave enough to microwave it, and water baths are too much hassle, so I haven't tried heating it.

I stirred the bejesus out of it; the powder was extremely fine, which helped. It was not clear, so may have been a suspension with very small particle size.

Is it really necessary to dissolve it? Doesn't it precipitate out as soon as it mixes with anything else? My current approach is to add 5ml 95% ETH to 2 grams and stir (I used to use 7ml Everclear which is 75% ETH). All of the powder mixes with the ethanol to form a white liquid. I then add 4 oz. of pomegranate juice and stir. I end up with a nice purple liquid with just a few white specs here and there. I assume that I have a very fine suspension. Not the best tasting thing in the world but quite drinkable.

[niner]

I rather like this recipe:

-Dissolve 3 grams of resveratrol in 30 ml of 80 proof vodka, set aside. (or use 90% ethanol, adjust proportions)
-take a heaping tsp of lecithin and add to one cup of water; mix until dissolved. Use of a blender speeds the process. I use a Braun mini-primer with mixing jar and blade. Any mini-blender should be good.
-Strain the lecithinated water to remove any lumps of lecithin.
-Add the resveratrol-vodka mix to the water and stir.

Maxwatt, can you really get 3 grams into 30ml of 80 proof vodka (40% EtOH)? I use Everclear (95% EtOH), and I don't think I can get 3 grams into 30ml of that. Maybe 2 grams, or a little more, but it's more than twice as concentrated in EtOH... I've never been brave enough to microwave it, and water baths are too much hassle, so I haven't tried heating it.

I stirred the bejesus out of it; the powder was extremely fine, which helped. It was not clear, so may have been a suspension with very small particle size.

Is it really necessary to dissolve it? Doesn't it precipitate out as soon as it mixes with anything else? My current approach is to add 5ml 95% ETH to 2 grams and stir (I used to use 7ml Everclear which is 75% ETH). All of the powder mixes with the ethanol to form a white liquid. I then add 4 oz. of pomegranate juice and stir. I end up with a nice purple liquid with just a few white specs here and there. I assume that I have a very fine suspension. Not the best tasting thing in the world but quite drinkable.

If you don't dissolve it, then you'll get whatever particle size remains after some of it has been dissolved. The thinking behind dissolving it and then precipitating it in water (an anti-solvent, in this case) is that the particles will be extremely fine. The lecithin or PEG should in theory stabilize the very small particles and prevent agglomeration. I'm not sure how well this really works; at least with PEG I find that the suspension changes over time (on the order of minutes) and does seem to be agglomerating. I haven't tried lecithin, so I don't know what happens there. I looked at the suspension under a light microscope, and saw what appeared to be loose assemblages of much smaller particles. These might have the same practical effect as the initial smaller particles, or not; I don't know. I mix with about 4oz of PEG solution, then add fruit juice. I drink it quickly so it has less time to agglomerate. I figure that the more dilute it is (in the stomach, in this case) the less agglomeration I should see. It doesn't taste that great, does it?

ps: Everclear is 95%.

[maxwatt]

ps: Everclear is 95%.

In some states the highest proof one can sell is 151, so in those places Everclear is 75% alcohol (150 proof).

edited for speling and gramer

Edited by maxwatt, 27 January 2008 - 04:16 PM.

[renwosing]

How about resveratrol with HGH?

Wonder if anybody has done it before.

Renwosing

[health_nutty]

Why have we seemed to abandon the transdermal application of resveratrol in DMSO? It seems like bypassing the intestine's metabolizing enzymes would be a huge benefit to getting a larger peak serum resveratrol concentration. I mean its got to be better just to deal with the liver metabolizing resveratrol that BOTH the intestines and the liver.

[missminni]

Why have we seemed to abandon the transdermal application of resveratrol in DMSO? It seems like bypassing the intestine's metabolizing enzymes would be a huge benefit to getting a larger peak serum resveratrol concentration. I mean its got to be better just to deal with the liver metabolizing resveratrol that BOTH the intestines and the liver.

I haven't abandon DMSO. I've been rubbing it on Busta's infected ear as well
as giving it to him internally. I also have been rubbing it on the site of Minni's surgery since she is shaved in that area.
She is recovering beautifully. I also have her on 6g orally (she is 97lbs) a day to fight the cancer she was diagnosed with,
and have Busta on 3g a day orally (he is 125lbs).
I still use it transdermally myself as well as orally (3g a day...I weigh the same as Busta). I figure that way you
get the benefits of both methods.

[sUper GeNius]

Why have we seemed to abandon the transdermal application of resveratrol in DMSO? It seems like bypassing the intestine's metabolizing enzymes would be a huge benefit to getting a larger peak serum resveratrol concentration. I mean its got to be better just to deal with the liver metabolizing resveratrol that BOTH the intestines and the liver.

I haven't abandon DMSO. I've been rubbing it on Busta's infected ear as well
as giving it to him internally. I also have been rubbing it on the site of Minni's surgery since she is shaved in that area.
She is recovering beautifully. I also have her on 6g orally (she is 97lbs) a day to fight the cancer she was diagnosed with,
and have Busta on 3g a day orally (he is 125lbs).
I still use it transdermally myself as well as orally (3g a day...I weigh the same as Busta). I figure that way you
get the benefits of both methods.

Have you noticed any side effects with the dosages your are giving the dogs? How long have you been dosing them? How long at the highest dose?

I have started my 70lb dog on about 150mg a day, for the last week or so. How did you arrive at the 3g dose for Busta? How old is Busta? Have you noticed any benefits?

[missminni]

Have you noticed any side effects with the dosages your are giving the dogs?
No bad ones. Maybe their stool is a bit looser than normal. Prior to high dosing, I was giving them about 200 mg-400 mg a day for about 6 months on and off.
Busta had an ongoing (4yr) ear infection that basically cleared up within 24 hours of his first 3g dose (1.5 g x2) The discharge
changed from dark brown (dried blood goop) to amber. He also immediately stopped drooling brown drool, which had been
going on for 4years and I now realize was from his ear infection. No antibiotics could ever get rid of it. It is not even a week later and the clear amber discharge has diminished
every day and is now almost totally gone.
How long have you been dosing them?
They were on 200mg for about 6 months. No results good or bad. I started high dosing Minni on Jan 18th
the day I found out she had terminal cancer and was given a death sentence of 6 months. I have been giving her between 5 to 6 grams a day ever since. No bad side effects at all. She looks great and is healing beautifully from her mastectomy. Her eyes look stronger than normal. Busta has been hi dosing since Jan 22.
How long at the highest dose? One and a half weeks
I have started my 70lb dog on about 150mg a day, for the last week or so.How did you arrive at the 3g dose for Busta? How old is Busta? Have you noticed any benefits?
Busta and Minni turn 9 years old on Monday. I arrived at 3 grams for Busta because if I gave him the 10lb/100mg ratio of his weight, it would be 1.25 g. Since he had something wrong with him that I was trying to cure, I decided to double it and add some. I had the experience that the more you take the more good it does and since he is 9 years old and afterall, look what happened to Minni from not being fixed...Busta's not fixed either. They say dogs that are intact get cancer more frequently. I am doing it as precautionary measure. I am taking 3 grams too. I don't think it that high considering what was used in the experiments. If your dog is old, I would give him more.
At 70lb I would give at least 700mg and if he's an older dog and intact, I would give him at least a gram to 1.5 g. Especially if he has any ailments. Minni's only 97 lbs and I am giving her 6 grams cause I am trying to beat cancer. It all depends.

I've answered within the quote.

[sUper GeNius]

Have you noticed any side effects with the dosages your are giving the dogs?
No bad ones. Maybe their stool is a bit looser than normal. Prior to high dosing, I was giving them about 200 mg-400 mg a day for about 6 months on and off.
Busta had an ongoing (4yr) ear infection that basically cleared up within 24 hours of his first 3g dose (1.5 g x2) The discharge
changed from dark brown (dried blood goop) to amber. He also immediately stopped drooling brown drool, which had been
going on for 4years and I now realize was from his ear infection. No antibiotics could ever get rid of it. It is not even a week later and the clear amber discharge has diminished
every day and is now almost totally gone.
How long have you been dosing them?
They were on 200mg for about 6 months. No results good or bad. I started high dosing Minni on Jan 18th
the day I found out she had terminal cancer and was given a death sentence of 6 months. I have been giving her between 5 to 6 grams a day ever since. No bad side effects at all. She looks great and is healing beautifully from her mastectomy. Her eyes look stronger than normal. Busta has been hi dosing since Jan 22.
How long at the highest dose? One and a half weeks
I have started my 70lb dog on about 150mg a day, for the last week or so.How did you arrive at the 3g dose for Busta? How old is Busta? Have you noticed any benefits?
Busta and Minni turn 9 years old on Monday. I arrived at 3 grams for Busta because if I gave him the 10lb/100mg ratio of his weight, it would be 1.25 g. Since he had something wrong with him that I was trying to cure, I decided to double it and add some. I had the experience that the more you take the more good it does and since he is 9 years old and afterall, look what happened to Minni from not being fixed...Busta's not fixed either. They say dogs that are intact get cancer more frequently. I am doing it as precautionary measure. I am taking 3 grams too. I don't think it that high considering what was used in the experiments. If your dog is old, I would give him more.
At 70lb I would give at least 700mg and if he's an older dog and intact, I would give him at least a gram to 1.5 g. Especially if he has any ailments. Minni's only 97 lbs and I am giving her 6 grams cause I am trying to beat cancer. It all depends.

I've answered within the quote.

My dog, a male, has been fixed, (?) He has no serious ailments that I can see. He's 10. I have noticed a general stiffening, slowness, that I attribute to arthritis. He used to beg for a walk every single day. These days, although he doesn't turn a walk down, (still gets very excited when asked,) he never stands by the door to beg for a walk. I assume that's because walking hurts a bit.

How did you arrive at the 10lb/100mg ratio?

[missminni]

My dog, a male, has been fixed, (?) He has no serious ailments that I can see. He's 10. I have noticed a general stiffening, slowness, that I attribute to arthritis. He used to beg for a walk every single day. These days, although he doesn't turn a walk down, (still gets very excited when asked,) he never stands by the door to beg for a walk. I assume that's because walking hurts a bit.

How did you arrive at the 10lb/100mg ratio?

I read about the 10lb/100mg ratio here. Although I've been told Resveratrol makes glucosamine
redundant, I still give my dogs glucosamine sulfate at the ratio of 1g from every 33lbs of bodyweight. Prior to knowing that, I was just giving them 1500 mg a day. Busta was still stiff and having trouble going up stairs. The same day I gave him 4 grams of glucosamine, he ran up the stairs, so I continue glucosamine along with the Res. If your dog is stiff, I suggest you give him glucosamine. At 70 lbs I would give him 2.5 grams a day.

[maxwatt]

My dog, a male, has been fixed, (?) He has no serious ailments that I can see. He's 10. I have noticed a general stiffening, slowness, that I attribute to arthritis. He used to beg for a walk every single day. These days, although he doesn't turn a walk down, (still gets very excited when asked,) he never stands by the door to beg for a walk. I assume that's because walking hurts a bit.

How did you arrive at the 10lb/100mg ratio?

I read about the 10lb/100mg ratio here. Although I've been told Resveratrol makes glucosamine
redundant, I still give my dogs glucosamine sulfate at the ratio of 1g from every 33lbs of bodyweight. Prior to knowing that, I was just giving them 1500 mg a day. Busta was still stiff and having trouble going up stairs. The same day I gave him 4 grams of glucosamine, he ran up the stairs, so I continue glucosamine along with the Res. If your dog is stiff, I suggest you give him glucosamine. At 70 lbs I would give him 2.5 grams a day.

Glucosamine might not be redundant. It inhibits nf_Kappa B. As do creatine and silymarin. as well as resveratrol.