1230 replies to this topic

[TRUGAN]

 

I agree that herbal synergies is becoming something of a lost 'art' and think we would do well to look more closely at oriental, ayurvedic etc combinations.

 

One certainly does not want to take out beneficial bacteria and biofilms!  Hence bacteriophages:

These are virii that live on bacteria and are very fussy/specific to the bacteria they 'consume'.

A case in point is M13 which not only kills E. Coli but serendipitously also 'dissolves' amyloid-beta plaques!

http://www.pbs.org/w...lzheimers-cure/

 

 

 

 

 

Thats very interesting about M13. Whats to stop folks from sticking this up their noses?  I guess its probably not for sale on any of the sites like http://www.sigmaaldrich.com/ ?

 

 

Edited by mrwhitee, 08 July 2016 - 03:25 AM.

[Logic]

 

 

I agree that herbal synergies is becoming something of a lost 'art' and think we would do well to look more closely at oriental, ayurvedic etc combinations.

 

One certainly does not want to take out beneficial bacteria and biofilms!  Hence bacteriophages:

These are virii that live on bacteria and are very fussy/specific to the bacteria they 'consume'.

A case in point is M13 which not only kills E. Coli but serendipitously also 'dissolves' amyloid-beta plaques!

http://www.pbs.org/w...lzheimers-cure/

 

 

 

 

 

Thats very interesting about M13. Whats to stop folks from sticking this up their noses?  I guess its probably not for sale on any of the sites like http://www.sigmaaldrich.com/ ?

 

 

 

Nothing whatsoever IMHO.
The drug being developed from the research is nothing more than an attempt to patent/commercialise an unpatentable viral solution.

The thought of purposely  snarfing a virus does elicit all sorts of mental alarm bells though!   

My time is limited atm, but I do plan to find this and other phages and organise a group buy.
The thought of full control over one's microbiota (No LPS etc or unwanted nutrienst) is very exciting IMHO!
Getting them alive and into one's gut is likely to pose some ...challenges..!

Edited by Logic, 08 July 2016 - 12:33 PM.

[TRUGAN]

 

 

 

I agree that herbal synergies is becoming something of a lost 'art' and think we would do well to look more closely at oriental, ayurvedic etc combinations.

 

One certainly does not want to take out beneficial bacteria and biofilms!  Hence bacteriophages:

These are virii that live on bacteria and are very fussy/specific to the bacteria they 'consume'.

A case in point is M13 which not only kills E. Coli but serendipitously also 'dissolves' amyloid-beta plaques!

http://www.pbs.org/w...lzheimers-cure/

 

 

 

 

 

Thats very interesting about M13. Whats to stop folks from sticking this up their noses?  I guess its probably not for sale on any of the sites like http://www.sigmaaldrich.com/ ?

 

 

 

Nothing whatsoever IMHO.
The drug being developed from the research is nothing more than an attempt to patent/commercialise an unpatentable viral solution.

The thought of purposely  snarfing a virus does elicit all sorts of mental alarm bells though!   

My time is limited atm, but I do plan to find this and other phages and organise a group buy.
The thought of full control over one's microbiota (No LPS etc or unwanted nutrienst) is very exciting IMHO!
Getting them alive and into one's gut is likely to pose some ...challenges..!

 

 

 

I will definitely do a group buy on these phages as long as is not 20,000.00. :-)    Im not too worried about snorting them up the nose. They only infect e coli and any of the bad stuff in our brains so sign me up for a billion or so of these phages.  The sooner the better. :-)

[Mian Ali Ismail]

Pulmozyme is minimally absorbed in rats.Does anybody here know about its oral Absorbtion in humans ?

[Logic]

That's interesting. I'll definitely have to look into that more. I didn't realize they could do a quick screening of many phages like that.

 

Yes; it would seem that phages are pretty ubiquitous and responsible for the healing properties of certain rivers/healing waters etc.
It would seem that we would be up to our eyeballs in bacterial blooms if it was not for phages.

Phages are anathema for any business built on fermentation processes and they go to great lengths to guard against phage contamination.
This means that the largest phage varieties tend to congregate around the largest various sources of bacteria, like in hospital drains and cemeteries!
This is in fact the russian source of the wide range of phages added to the purified bacteria one wants to kill!
ie: in short; add hospital drain water to the isolated/purified bacteria/s, see what grows and isolate/purify that!

Gross, but there it is!  

It's actually a very elegant, russian style, solution to a very difficult problem!

 

Do they flat out kill an entire species? Or is it concentration dependent so you can just dial it back to a healthy balance? I don't tend to think there are bad players so much as simple imbalances in the ecology

 
I have wondered about this myself.
My initial impression is that the phage will infect/kill all the bacteria and then die off itself, so one would need to reacquire another dose if one was re-infected. (Altogether possible when living in a family unit and a good reason to dose everyone..!?)
It would be nice if a bacteria/phage balance was reached with just a couple of bacteria surviving to keep the phages alive.
I don't know if this is the case, or would be the case in the gut..?
As always; more research required, but well worth it for dementia suffers and life extension in general IMHO.

 

@ "needs references"
Open Google; type in: 'M13 bacteriophage amyloid beta pubmed'

Magically, the following results appear!  

https://www.google.c...loid betapubmed
 

Edited by Logic, 09 July 2016 - 01:27 PM.

[TRUGAN]

No luck over here looking for phages. If we can find a source we could easily freeze them so that we have future doses. 

[TRUGAN]

found this interesting paper on phage therapy.

 

http://www.microbiol...A292E1C08ED58B5

 

 

Also, looks like you can find m13 in cow poop and maybe your own poop.

 

 

Those willing to travel can go to the phage therapy center.

 

http://www.phagether...tact&language=0

 

 

Article about them:

 

https://www.buzzfeed...A92J#.rqLXYmkeE

 

 

I'd rather have my phages mailed to me though.

 

 

 

[TRUGAN]

I just received an email from the phage center in Russia and they do not have the M13 phage so I guess thats it for that. :-(

[TRUGAN]

Isolate your own m13 from sewer water.

 

 

 

Its almost too easy but of course this is coming from someone thats never done it so maybe it just looks easy

 

 

Petri dishes and Agar cost almost nothing. Sewer water is free. Perhaps our own sewer water at the house contain the phages.

 

 

I know one of you will click that dangerous button but getting out of bed can be dangerous as well so stop bitching..

[Der Springende Punkt]

New possible target:Kallikrein-8 inhibition

 

Kallikrein-8 inhibition attenuates Alzheimer's pathology in mice

 

If anyone finds some available substances with Kallikrein-8 inhibition properties feel free to post them.

 

[Mian Ali Ismail]

Alzhimers patient improved with CT scans treatment as form of low dose radiatio,\.

 

http://www.ncbi.nlm....les/PMC4826954/

[corb]

Alzhimers patient improved with CT scans treatment as form of low dose radiatio,\.

 

http://www.ncbi.nlm....les/PMC4826954/

 

Hormesis. Makes me very hopeful for the senolytic testing on mice in the MMTP.
Senolytics should have the upper hand over radiation because they're targeted.

 

After 5 seconds just as I expected:

http://www.ncbi.nlm....cles/PMC3388011

http://www.ncbi.nlm....pubmed/25666032

 

Both Quercetin and Dasatinib show AD ameliorating potential in mice. Coincidence? Heh, maybe, but maybe not.
 

[mag1]

Wanted to make a note of these ones.

MKT-077 repurposed to YM-08 for possible neurodegenerative uses by reducing tau levels.

 

http://www.ncbi.nlm....pubmed/23472668

 

HP-B-cyclodextrin is on the shelf at Sigma.

http://www.ncbi.nlm....pubmed/27405335

Edited by mag1, 17 July 2016 - 09:56 PM.

[resveratrol_guy]

Not sure this fits in here but nevertheless I though useful to log it in this thread. I think previous work by some of the authors was discussed (and criticized) on LC, e.g. here, here.

 

A multi-ingredient dietary supplement abolishes large-scale brain cell loss, improves sensory function, and prevents neuronal atrophy in aging mice

http://onlinelibrary....22019/abstract

 

"Transgenic growth hormone mice (TGM) are a recognized model of accelerated aging with characteristics including chronic oxidative stress, reduced longevity, mitochondrial dysfunction, insulin resistance, muscle wasting, and elevated inflammatory processes. Growth hormone/IGF-1 activate the Target of Rapamycin known to promote aging. TGM particularly express severe cognitive decline. We previously reported that a multi-ingredient dietary supplement (MDS) designed to offset five mechanisms associated with aging extended longevity, ameliorated cognitive deterioration and significantly reduced age-related physical deterioration in both normal mice and TGM. Here we report that TGM lose more than 50% of cells in midbrain regions, including the cerebellum and olfactory bulb. This is comparable to severe Alzheimer's disease and likely explains their striking age-related cognitive impairment. We also demonstrate that the MDS completely abrogates this severe brain cell loss, reverses cognitive decline and augments sensory and motor function in aged mice. Additionally, histological examination of retinal structure revealed markers consistent with higher numbers of photoreceptor cells in aging and supplemented mice. We know of no other treatment with such efficacy, highlighting the potential for prevention or amelioration of human neuropathologies that are similarly associated with oxidative stress, inflammation and cellular dysfunction."

 

It is not open access but I included it in the list of readings, assuming it is worth, next time in the library.

 

FYI the formula for the MDS supplement is here. Note the comparatively huge dose of N-acetyl-cysteine, which is kind of prophetic considering that this was 2005, when the connection between cytoplasmic cysteine and respiratory efficiency was still unknown. Still trying to dig myself out from under a hundred studies to which people were kind enough to alert me...
 

I also just stumbled upon this paper which sounds like licorice root extract inhibits tau fibrilization. I have no time to read it all right now, but it sounded too good to ignore.

Edited by resveratrol_guy, 23 July 2016 - 07:17 AM.

[mag1]

CRISPRing obviously changes everything!

The technology that redefines humanity and every imaginable disease or condition.  

 

https://www.theguard...ng-trial-humans

[resveratrol_guy]

 

Alzhimers patient improved with CT scans treatment as form of low dose radiatio,\.

 

http://www.ncbi.nlm....les/PMC4826954/

 

Hormesis. Makes me very hopeful for the senolytic testing on mice in the MMTP.
Senolytics should have the upper hand over radiation because they're targeted.

 

After 5 seconds just as I expected:

http://www.ncbi.nlm....cles/PMC3388011

http://www.ncbi.nlm....pubmed/25666032

 

Both Quercetin and Dasatinib show AD ameliorating potential in mice. Coincidence? Heh, maybe, but maybe not.

 

Actually, in this case, I think we want an untargetted approach, so as to activate hormesis generally. That would tend to reduce all forms of pathology, including those outside the domain of senolytics, which are largely upregulating autophagy and apoptosis. Low-dose radiation might even kill virusses, which have no protection to speak of, and perhaps directly disaggregate plaques by breaking chemical bonds. It would also likely destroy cells in the midst of severe energy deficits on the verge of turning glycolytic and thus carcinogenic.

 

The authors stated that after the fifth and final CT scan, the patient's mental state temporarily declined quite sharply, only to improve later. They interpreted this as an indication of exposure in excess of the amounts in which beneficial hormesis would be expected. This might be the case because, although weeks had elapsed between the last 2 scans, affording plenty of healing time, the patient's advanced age may have inhibited healing to such a degree as to require even more time between exposures. This is just to point out that there are limitations to this approach, and as always, starting while younger and healthier would likely produce better outcomes. Nevertheless I find it difficult to imagine young or even middle aged people taking monthly brain CTs just for the sake of achieving beneficial hormesis, particularly because we have no good way to assess whether or not the radiation was more helpful than harmful, to say nothing of the financial implications.

 

As the authors pointed out, however, this suggests the possibility of using ultrasound to activate hormesis, which is much cheaper and could probably be done using an off-the-shelf device, in a manner similar to home-based LLLT. But I don't believe we have the same life extension data for ultrasound that we do for xrays, and the former disrupts the BBB, whereas the latter produces diffuse chemical damage of the sort which hormesis evolved to mitigate. The payoff could nonetheless be enormous if we could stave off dementia for a few years using such a device, so this is surely worth some research.

[corb]

 

 

Alzhimers patient improved with CT scans treatment as form of low dose radiatio,\.

 

http://www.ncbi.nlm....les/PMC4826954/

 

Hormesis. Makes me very hopeful for the senolytic testing on mice in the MMTP.
Senolytics should have the upper hand over radiation because they're targeted.

 

After 5 seconds just as I expected:

http://www.ncbi.nlm....cles/PMC3388011

http://www.ncbi.nlm....pubmed/25666032

 

Both Quercetin and Dasatinib show AD ameliorating potential in mice. Coincidence? Heh, maybe, but maybe not.

 

Actually, in this case, I think we want an untargetted approach, so as to activate hormesis generally. That would tend to reduce all forms of pathology, including those outside the domain of senolytics, which are largely upregulating autophagy and apoptosis. Low-dose radiation might even kill virusses, which have no protection to speak of, and perhaps directly disaggregate plaques by breaking chemical bonds. It would also likely destroy cells in the midst of severe energy deficits on the verge of turning glycolytic and thus carcinogenic.

 

The authors stated that after the fifth and final CT scan, the patient's mental state temporarily declined quite sharply, only to improve later. They interpreted this as an indication of exposure in excess of the amounts in which beneficial hormesis would be expected. This might be the case because, although weeks had elapsed between the last 2 scans, affording plenty of healing time, the patient's advanced age may have inhibited healing to such a degree as to require even more time between exposures. This is just to point out that there are limitations to this approach, and as always, starting while younger and healthier would likely produce better outcomes. Nevertheless I find it difficult to imagine young or even middle aged people taking monthly brain CTs just for the sake of achieving beneficial hormesis, particularly because we have no good way to assess whether or not the radiation was more helpful than harmful, to say nothing of the financial implications.

 

As the authors pointed out, however, this suggests the possibility of using ultrasound to activate hormesis, which is much cheaper and could probably be done using an off-the-shelf device, in a manner similar to home-based LLLT. But I don't believe we have the same life extension data for ultrasound that we do for xrays, and the former disrupts the BBB, whereas the latter produces diffuse chemical damage of the sort which hormesis evolved to mitigate. The payoff could nonetheless be enormous if we could stave off dementia for a few years using such a device, so this is surely worth some research.

 

 

Here we go a bit into the semantics and lack of standardization of terms in biology.
Senolytics even though they are targeted, are still considered to be working through a hormetic response.
I think targeted is better because : it lowers inflammation by removing the cause (or one cause), it improves the immune system, and it does it by only killing cells which should be useless, something which I generally consider better especially in the brain.

But I see your point as well. I had considered low doses of cancer therapy as a makeshift senolytic treatments alternative couple of years ago when targeted therapies were simply proposed possibilities. Granted most senolytics seem to be cancer drugs and derivatives.

Maybe we could use cancer research as a baseline comparison?

[resveratrol_guy]

Here we go a bit into the semantics and lack of standardization of terms in biology.

Senolytics even though they are targeted, are still considered to be working through a hormetic response.
I think targeted is better because : it lowers inflammation by removing the cause (or one cause), it improves the immune system, and it does it by only killing cells which should be useless, something which I generally consider better especially in the brain.

But I see your point as well. I had considered low doses of cancer therapy as a makeshift senolytic treatments alternative couple of years ago when targeted therapies were simply proposed possibilities. Granted most senolytics seem to be cancer drugs and derivatives.

Maybe we could use cancer research as a baseline comparison?

 

That's a good idea. Test cancer patients receiving various senolytics to ascertain their cognitive outcomes. Of course, this is complicated by the brain damage induced by the ordeal of cancer itself, including all the other medications such patients often consume. But probably we could control for that. The problem is that it would be unethical to provide fake cancer drugs to a control group, so we would have to rely on dose response to say something useful.

 

I don't think we want to kill normal brain cells. However, I actually think we should irradiate all of them, gently and once in a while. This would kill off dysfunctional mitochondria and activate their repair processes. Again, I have no idea how to define "gently" for any particular person, although it seems we can define the within a factor of 2 or so based on current research.

 

At least, it seems that we have a "salvage therapy" of some value for later stage patients, who could easily justify obtaining a brain CT for general diagnostic reasons.

 

In my personal experience, CT technicians can only provide you with exact dosimetry after the scan. However, they usually know the approximate range of radiation doses beforehand. But beware that different scanners can produce wildly different dosages. For the purposes of treating dementia, the patient should be wearing a lead collar and a lead apron to shield everything except the brain. I would even recommend draping a lead apron over the mouth and jaws, which have no reason to be irradiated.

 

Ali, would you consider giving your dad such a CT? It will probably cost between $100 and $1000.

 

[resveratrol_guy]

I noticed that M13K07 helper phage is available here and other places for a few hundred dollars. It's resistant to kanamycin, which is not good but probably not intractable, as it's presumably susceptible to other antibiotics in case it gets out of control. Can anyone determine whether or not it's sufficiently compatible with wild type M13 to "unlock" amyloid and tau fibrils? Specifically, it needs to display the GP3 protein.

 

It sounds like NeuroPhage is pursuing a hopeless pharmaceutical approach by making a drug with poor BBB permeability, which they hope will somehow penetrate the brain as pervasively as a real phage. Presumably phages have some sort of locomotion; even HIV has this feature. But a drug probably does not, so it seems unlikely to achieve sufficient perfusion. Of course they're doing this because they can't patent M13. I wish they would patent a novel delivery mechanism instead of running off into the weeds in search of unlikely treatments, while more people die. The incentives in this business are completely inverted from a social benefit maximization standpoint.

[TRUGAN]

I noticed that M13K07 helper phage is available here and other places for a few hundred dollars. It's resistant to kanamycin, which is not good but probably not intractable, as it's presumably susceptible to other antibiotics in case it gets out of control. Can anyone determine whether or not it's sufficiently compatible with wild type M13 to "unlock" amyloid and tau fibrils? Specifically, it needs to display the GP3 protein.

 

It sounds like NeuroPhage is pursuing a hopeless pharmaceutical approach by making a drug with poor BBB permeability, which they hope will somehow penetrate the brain as pervasively as a real phage. Presumably phages have some sort of locomotion; even HIV has this feature. But a drug probably does not, so it seems unlikely to achieve sufficient perfusion. Of course they're doing this because they can't patent M13. I wish they would patent a novel delivery mechanism instead of running off into the weeds in search of unlikely treatments, while more people die. The incentives in this business are completely inverted from a social benefit maximization standpoint.

 

 

Not sure about that helper phage but I am attempting to isolate the m13 from sewer water. I am still in the process of collecting needed supplies. The refrigerated centrifuge seems to be the only equipment needed and I have just received that from ebay and of course it doesnt work as advertised but I have identified the issue and have parts on order to repair. Hopefully I will be making the first attempt in 2 or 3 weeks.

 

One thing to consider is if enough of the phage can travel up the nose to do any good. Maybe if its snorted every day for a long period of time.

[Mian Ali Ismail]

 

Here we go a bit into the semantics and lack of standardization of terms in biology.

Senolytics even though they are targeted, are still considered to be working through a hormetic response.
I think targeted is better because : it lowers inflammation by removing the cause (or one cause), it improves the immune system, and it does it by only killing cells which should be useless, something which I generally consider better especially in the brain.

But I see your point as well. I had considered low doses of cancer therapy as a makeshift senolytic treatments alternative couple of years ago when targeted therapies were simply proposed possibilities. Granted most senolytics seem to be cancer drugs and derivatives.

Maybe we could use cancer research as a baseline comparison?

 

That's a good idea. Test cancer patients receiving various senolytics to ascertain their cognitive outcomes. Of course, this is complicated by the brain damage induced by the ordeal of cancer itself, including all the other medications such patients often consume. But probably we could control for that. The problem is that it would be unethical to provide fake cancer drugs to a control group, so we would have to rely on dose response to say something useful.

 

I don't think we want to kill normal brain cells. However, I actually think we should irradiate all of them, gently and once in a while. This would kill off dysfunctional mitochondria and activate their repair processes. Again, I have no idea how to define "gently" for any particular person, although it seems we can define the within a factor of 2 or so based on current research.

 

At least, it seems that we have a "salvage therapy" of some value for later stage patients, who could easily justify obtaining a brain CT for general diagnostic reasons.

 

In my personal experience, CT technicians can only provide you with exact dosimetry after the scan. However, they usually know the approximate range of radiation doses beforehand. But beware that different scanners can produce wildly different dosages. For the purposes of treating dementia, the patient should be wearing a lead collar and a lead apron to shield everything except the brain. I would even recommend draping a lead apron over the mouth and jaws, which have no reason to be irradiated.

 

Ali, would you consider giving your dad such a CT? It will probably cost between $100 and $1000.

 

The DR. ordered a CT scan for my dad the last year and also changed the meds.He had a lot of improvement like 30 % after the CT scan.We thought it might be because of the change in meds.We showed the DR.improvement as well.He also said tht it might be because of the meds changed.But I think it might be because of CT scan as he was already talking Dopamine and the only meds changed were memantine and one other I think a dopamine antagonist.

[resveratrol_guy]

The DR. ordered a CT scan for my dad the last year and also changed the meds.He had a lot of improvement like 30 % after the CT scan.We thought it might be because of the change in meds.We showed the DR.improvement as well.He also said tht it might be because of the meds changed.But I think it might be because of CT scan as he was already talking Dopamine and the only meds changed were memantine and one other I think a dopamine antagonist

 

If it was back in 2015, then it seems to me that plenty of time has elapsed for healing to occur. You could attempt another one now. I would recommend using exactly the same machine and doing exactly the same scan, but with the lead shielding that I suggested above.

 

A while ago I assembled a list of substances to consider taking for protection from xrays. However, in this case, you want some of the damage, which complicates the protocol. By default, I would recommend keeping him on the same diet as before, if possible. Then after a day or two, I would start supplementing items from the list in order to support the hormetic repair process. The main point is to allow the dysfunctional mitochondria and senescent cells to die in sufficient numbers to make a difference.

Edited by resveratrol_guy, 25 July 2016 - 05:19 AM.

[resveratrol_guy]

Not sure about that helper phage but I am attempting to isolate the m13 from sewer water. I am still in the process of collecting needed supplies. The refrigerated centrifuge seems to be the only equipment needed and I have just received that from ebay and of course it doesnt work as advertised but I have identified the issue and have parts on order to repair. Hopefully I will be making the first attempt in 2 or 3 weeks.

 

One thing to consider is if enough of the phage can travel up the nose to do any good. Maybe if its snorted every day for a long period of time.

 

The good news is that phages can reproduce, at least until they're killed by the immune system or a fever, so presumably we don't need to worry too much about how many of them get into the brain. And if they disaggregate plaques in humans as they do in mice, which I think is likely on account of the primitive nature of beta amyloid, they would be expected to produce an enduring cure that might need to be repeated only annually.

However, anyone attempting this needs to be aware of the hazards of snorting pathogens. If amoebas or certain bacteria cross the olfactory nerve into the brain, you're basically as good as dead because there is no treatment to stop some forms of encephalitis. The only way around this would be to isolate a few phages, then replicate them in media known to be sterile. This is certainly not beyond the realm of modern technology, but it demands high standards.

Realistically, though, we need to weigh the probability of acquiring fatal encephalitis from a few snorts of water containing few if any "bad" pathogens (very low, if you think about how many people swim in dirty rivers every day) against the probability that it will reduce your plaque load by enough to improve your life. That much is a personal decision.

In any case, how would you know if it worked? And by the way, do you really have the skills and equipment to identify M13 and be confident that it exposes the GP3 protein?

[TRUGAN]

 

Not sure about that helper phage but I am attempting to isolate the m13 from sewer water. I am still in the process of collecting needed supplies. The refrigerated centrifuge seems to be the only equipment needed and I have just received that from ebay and of course it doesnt work as advertised but I have identified the issue and have parts on order to repair. Hopefully I will be making the first attempt in 2 or 3 weeks.

 

One thing to consider is if enough of the phage can travel up the nose to do any good. Maybe if its snorted every day for a long period of time.

 

The good news is that phages can reproduce, at least until they're killed by the immune system or a fever, so presumably we don't need to worry too much about how many of them get into the brain. And if they disaggregate plaques in humans as they do in mice, which I think is likely on account of the primitive nature of beta amyloid, they would be expected to produce an enduring cure that might need to be repeated only annually.

However, anyone attempting this needs to be aware of the hazards of snorting pathogens. If amoebas or certain bacteria cross the olfactory nerve into the brain, you're basically as good as dead because there is no treatment to stop some forms of encephalitis. The only way around this would be to isolate a few phages, then replicate them in media known to be sterile. This is certainly not beyond the realm of modern technology, but it demands high standards.

Realistically, though, we need to weigh the probability of acquiring fatal encephalitis from a few snorts of water containing few if any "bad" pathogens (very low, if you think about how many people swim in dirty rivers every day) against the probability that it will reduce your plaque load by enough to improve your life. That much is a personal decision.

In any case, how would you know if it worked? And by the way, do you really have the skills and equipment to identify M13 and be confident that it exposes the GP3 protein?

 

 

 

I believe they only reproduce in e-coli so many virons are needed but Im sure a fresh batch will yield plenty . Even if you bought it online you'd still have contamination concerns because its not for human use so one just has to be careful to properly purify the isolate. If I did buy it online I think I would purify it further myself unless I had good reason to trust them.

[mag1]

Love to hear suggestions about how CRISPR might be used to prevent or treat AD.

Genetically engineering T-cells .... ?

 

This is a very exciting development.

[TRUGAN]

Maybe toking some THC every day might help.

 

http://www.cnn.com/2...uana/index.html

 

 

As the article and many others point out, we still dont know if removing plaques will even help. It may be another wild goose chase.

 

 

[TRUGAN]

Monoclonal Antibody with GP3

 

This is similar to what they want to patent.

 

https://www.exalpha....ts/detail/Z115M

 

Monoclonal Antibody

[TRUGAN]

I noticed that M13K07 helper phage is available here and other places for a few hundred dollars. It's resistant to kanamycin, which is not good but probably not intractable, as it's presumably susceptible to other antibiotics in case it gets out of control. Can anyone determine whether or not it's sufficiently compatible with wild type M13 to "unlock" amyloid and tau fibrils? Specifically, it needs to display the GP3 protein.

 

 

 

 

It appears to actually be suitable and express GP3 but I believe all the wild strains capable of infecting e-coli do express GP3 and this is definitely capable of infecting e-coli.  I cant be a 100% sure but I believe this would work identical to the wild type. I would never tell someone to use this on anything other than a rat....

 

One could easily buy this and grow more of it for continued use in rat testing. Storage should be in an ultra cold freezer which can be bought used from ebay. However, its not really that expensive so it may not be worth all that trouble to grow more.

Edited by mrwhitee, 26 July 2016 - 12:21 AM.

[resveratrol_guy]

 

I noticed that M13K07 helper phage is available here and other places for a few hundred dollars. It's resistant to kanamycin, which is not good but probably not intractable, as it's presumably susceptible to other antibiotics in case it gets out of control. Can anyone determine whether or not it's sufficiently compatible with wild type M13 to "unlock" amyloid and tau fibrils? Specifically, it needs to display the GP3 protein.

 

 

 

 

It appears to actually be suitable and express GP3 but I believe all the wild strains capable of infecting e-coli do express GP3 and this is definitely capable of infecting e-coli.  I cant be a 100% sure but I believe this would work identical to the wild type. I would never tell someone to use this on anything other than a rat....

 

One could easily buy this and grow more of it for continued use in rat testing. Storage should be in an ultra cold freezer which can be bought used from ebay. However, its not really that expensive so it may not be worth all that trouble to grow more.

 

 

Well, I hope said rat derives some benefit from your experiment. Let us know how it turns out.
 

[TRUGAN]

I or my rat have no reason to use it so it will go in the deep freeze if I am successful at making it. Next, I will look at making other useful phages that might be good for general health. So far all I have seen on the market is the product floraphage but I'm not convinced it's all that beneficial.