53 replies to this topic

[APBT]

Suggestive paper, but I am not holding my breath waiting for human studies. What oral dose would give a serum concentration similar to the in vitro concentration seen in the slices of rat brain?

Great question, for which, unfortunately I have know definitive answer. I do recall reading that ~40 strawberries yielded ~75 mg of fisetin, the amount of pure fisetin used in one of the studies.

I'm considering stacking several of the compounds (resveratrol, leutolin, pterostilbene, fiseten etc...) listed in your intriguing post
Effect of 36 Polyphenols on SIRT1 p53 deacetylase http://www.longecity...__fromsearch__1

I'm curious as the whether this would elicit a synergistic effect.

[maxwatt]

Great question, for which, unfortunately I have know definitive answer. I do recall reading that ~40 strawberries yielded ~75 mg of fisetin, the amount of pure fisetin used in one of the studies.

I'm considering stacking several of the compounds (resveratrol, leutolin, pterostilbene, fiseten etc...) listed in your intriguing post
Effect of 36 Polyphenols on SIRT1 p53 deacetylase http://www.longecity...__fromsearch__1

I'm curious as the whether this would elicit a synergistic effect.

I believe luteolin and resveratrol, in a 1:1 or 2:1 ratio, have a synergistic effect, greater than either alone. Also,, that luteolin by itself is as effective, or more, than resveratrol vis a vis endurance (mitochondrial boigenesis) and anti-arthritic effect. But this is not a scientific study, but observation of my self-experimentation. and the effect on my 13-year 0ld dog; she is more lively with luteolin in the mix, moves more easily than with resveratrol alone. And resveratrol is an improvement over her state with no supplementation.

[APBT]

I believe luteolin and resveratrol, in a 1:1 or 2:1 ratio, have a synergistic effect, greater than either alone. Also,, that luteolin by itself is as effective, or more, than resveratrol vis a vis endurance (mitochondrial boigenesis) and anti-arthritic effect. But this is not a scientific study, but observation of my self-experimentation. and the effect on my 13-year 0ld dog; she is more lively with luteolin in the mix, moves more easily than with resveratrol alone. And resveratrol is an improvement over her state with no supplementation.

Currently I'm using the (discontinued) RevGenetics Nitro-MX as my resveratrol source. I'm open to other source suggestions though.

One cap yields:
Trans Resveratrol 100 mg
Trans Resveratrol 3-O glucoside 100 mg
Quercetin 100 mg
Lecithin 390 mg
BioCurcumin 100 mg

I'm taking three caps in the AM prior to my workout. So, my total resveratrol intake is 600 mg. Based on your post, luteolin intake should be ~600 mg - 1200 mg?

I've ordered and yet to receive pterostilbene (pTeroPure). Any input on pterostilbene dosage?

I can't seem to source fisetin as a stand-alone product.

Interesting too that your pooch responds favorably to the luteolin/resveratrol combo. How much are you giving her daily?

[maxwatt]

pooch gets APPROXIMATELY 150 mg resveratrol, and/or 200 mg luteolin.

The quercetin in your pills may be counterproductive on the mitochondrial level, vis a vis ROS. I think 400 mg or more luteolin would be right with your regimen, though this is only a guess. Try titrating the dose, or use luteolin alone for a while.

If there is really a demand for fisetin, I may be able to source it, but I personally do not think it will work any better than say quercetin: It too does not appear to be a SIRT1 activator except under stabilizing conditions, which is not the case at the mitochondrial membrane under stress (ROS production.)

[Zaire]

Any updates on Fisetin?

[moomoo]

Any updates on Fisetin?

I have some Fisetin coming next week (http://www.amazon.co...g/dp/B0081NXW1C) and will let you know how I get on if there's anything notable to share.

[Gerald W. Gaston]

I'm using this uncapped as part of my 'berry elixir':

https://www.swansonv...-mg-30-veg-caps

[moomoo]

I'm using this uncapped as part of my 'berry elixir':

https://www.swansonv...-mg-30-veg-caps

Have you tried them outside of other nootropics and can you feel some benefit from just the Fisentin?

[moomoo]

Any updates on Fisetin?

I have some Fisetin coming next week (http://www.amazon.co...g/dp/B0081NXW1C) and will let you know how I get on if there's anything notable to share.

I have been taking this for three days so far.

My everyday weekday regime is:

• Noopept (about 20-30mg)
• Uridine TAU
• Maqui Berry
• Lypocene
• Cod-Liver Oil high strengh
• Ginseng
• Zinc
• Wellman multivitamin

When I have difficult work to do I'll add:

• Piracetam
• Hydergine

The Fisetin doesn't seem to be doing anything in addition to the above yet, but it could also be that the effect is being covered by everything else I'm taking. If any noticeable shows itself I'll update you all.

[moomoo]

Any updates on Fisetin?

I have some Fisetin coming next week (http://www.amazon.co...g/dp/B0081NXW1C) and will let you know how I get on if there's anything notable to share.

I have been taking this for three days so far.

My everyday weekday regime is:

• Noopept (about 20-30mg)
• Uridine TAU
• Maqui Berry
• Lypocene
• Cod-Liver Oil high strengh
• Ginseng
• Zinc
• Wellman multivitamin

When I have difficult work to do I'll add:

• Piracetam
• Hydergine

The Fisetin doesn't seem to be doing anything in addition to the above yet, but it could also be that the effect is being covered by everything else I'm taking. If any noticeable shows itself I'll update you all.

I've been dosing one tablet (100mg) every morning now since last week. Something is happening and it is around memory but it may be tied-in to wearing off of a cold. Seems to help my thinking somewhat - nothing dramatic, just a gentle easing like cycling with a slight tail-wind. Again, it might be simply to do with having battled a cold (which dulls my mental faculties) and getting over that, but it seems to be doing something subtle to me, mainly around my ability to multi-task (using short-term memory) and switching between tasks seems easier.

Too early to tell but I wanted to provide a report as promised.

Any others to report their experiences?

[dz93]

Anyone know where I can get just the powder not in capsules of this stuff? Also I prefer not to buy from China either. Thanks

[dz93]

No one at all?

[Castiel]

Is strawberries the highest food source?

[VERITAS INCORRUPTUS]

Actually, since both 10 and 25mg/kg showed significance it'd be 750-1875mg for 75kg person and 1000-2500...THEN adjust using the skin surface measurement (of which some debate, but is FDA tested and reference for big pharma) of about 6. 125mg-313mg for a 75kg person and 167mg-417mg of the fisetin.
http://www.pubmedcen...e...igure&id=F5
Using this table, there is a linear increase in cognitive effect with an exponential increase...aka diminishing returns with increasing doses. 25mg is not a dramatic difference between 10mg. To maybe top out...who knows...it could be further exponential of like 100mg/kg...and then you could run in to the down sides such as the mentioned DNA damage.

10mg/kg seems like a financially feasible and smart dose to experiment with. Buth either way study dosing in human terms would be about 100-500mg qd.

Compounds that exert effects that display such forms of dose response paradigms should generally be avoided as there is a relatively low 'positive therapeutic index'. Individual variance and species variance (as when such is only assayed for said effects in mice/rats) are important factor that as well further create a higher potential for dosing that is not only not optimal, but potentially of a deleterious nature; not merely simply suboptimal. This engenders a heightened risk:reward probability; as such, such substrates certainly require even more of a 'proceed with caution' disposition. If one is to proceed with usage, the most responsible path would be one to lean relatively severely on the low end dosing spectrum as so indicated within the results of the study.

Edited by VERITAS INCORRUPTUS, 27 February 2014 - 09:23 PM.

[toofast2live]

Hi there ive just bought some Fisetin, does anyone know if its best to take it on an empty stomach or with food?

Also, ive heard its synergistic with Resveratrol (possibly Pterastilbene?).

What do people think?

 

Thanks guys this is agreat forum

[stefan_001]

New study adds to evidence that Fisetin may protect against Alzheimer’s Disease

 

http://www.timelessl...eimers-disease/

 

Are there any long term users of Fisetin here? I have been using it for about 2 months and I have a sense my memory is functioning a tad better. Would love to hear other experiences to conclude whether its just placebo..

Edited by stefan_001, 13 April 2016 - 08:11 PM.

[eighthman]

https://selfhacked.c...tin-1-favorite/

 

I understand that fisetin may be senolytic.   As a speculation, I notice that fisetin seems to have a great many varied benefits across the body.  I would think that senescent cell clearance likewise has broad benefits in the same manner. Perhaps this breadth of benefits tells us that fisetin's benefits are the result of its senolytic action primarily.

[Florian E.]

https://selfhacked.c...tin-1-favorite/

 

I understand that fisetin may be senolytic.   As a speculation, I notice that fisetin seems to have a great many varied benefits across the body.  I would think that senescent cell clearance likewise has broad benefits in the same manner. Perhaps this breadth of benefits tells us that fisetin's benefits are the result of its senolytic action primarily.

 

I'm currently reading a lot about Fisetin and i think it will be one of my new favourite supplements.

 

Yes. Fisetin also seems to be senolytic, but not to all cell types.

See: https://www.ncbi.nlm...pubmed/28273655

 

 

It is not senolytic in senescent IMR90 cells, a human lung fibroblast strain, or primary human preadipocytes

 

 

But it seems that Fisetin could also be a better alternative to Rapamycin for "resetting epigenetics" !

Because Fisetin is a mTOR (mTORC1 & mTORC2) + NF-κB inhibitor (PI3K/AKT/mTOR pathway).

 

See:

https://www.ncbi.nlm...les/PMC4846973/

and

https://www.ncbi.nlm...les/PMC3539716/

 

According to other studies, inhibition of mTORC1/mTORC2 + NF-κB is enough to "reset" epigenetics/chromatin back to a proper state.

And with mTOR inhibition autophagy is increased so that less cells get senescent.

 

Plus, Fisetin does reduce DNA methylation.

 

Any current recommendations on Fisetin dosage for a strong therapeutic effect ??

[eighthman]

Good question about dosage.  I seem to recall that fisetin inhibits autophagy but maybe that's just temporary and it's longer term effects actually increase it.    

 

https://www.ncbi.nlm...pubmed/10969838

 

Maybe we can get rid of adipose cells by CLA.  Throw in some intermittent dasatinib/quercertin and who knows? We mere amateurs might be onto something big.

[John250]

Updates on this? On self decode it said it would be beneficial Supplement for me.

[Major Legend]

I love this compound, it works wonders for my really bad hospital level Eczema as part of a mast cell protocol I use for myself. I am only using it now because I am having a bad flare up, but in general, Fisetin is very expensive. Compile some research?

[John250]

I love this compound, it works wonders for my really bad hospital level Eczema as part of a mast cell protocol I use for myself. I am only using it now because I am having a bad flare up, but in general, Fisetin is very expensive. Compile some research?

It wasn’t that expensive here

https://www.amazon.c...O/dp/B0081NXW1C

[Major Legend]

It wasn’t that expensive here

https://www.amazon.c...O/dp/B0081NXW1C

 

This is the one I am using, it depends if you need higher dosage. 100mgX30 isn't a lot. Well it's a lot cheaper than I recalled from iherb.

[John250]

This is the one I am using, it depends if you need higher dosage. 100mgX30 isn't a lot. Well it's a lot cheaper than I recalled from iherb.

What dosage do you use?