12 replies to this topic

[health_nutty]

I just came across this and found it interesting. I haven't had a chance to dig into this at all. I knew olive oil had protective phytonutrients, I just never investigated the mechanism.

http://lib2.dss.go.t...e...:@12145&nx=

http://en.wikipedia.org/wiki/Tyrosol
As an antioxidant, tyrosol can protect cells against injury due to oxidation.[1] Although it is not as potent as other antioxidants present in olive oil, its higher concentration and good bioavailability indicate that it may have an important overall effect.[2] This effect may contribute significantly to the health benefits of olive oil and, more generally, the Mediterranean diet.

Recently Tyrosol present in white wine is also shown to be cardioprotective. Samson et al. has shown that tyrosol-treated animals showed significant increase in the phosphorylation of Akt, eNOS and FOXO3a. In addition, tyrosol also induced the expression of longevity protein SIRT1 in the heart after myocardial infarction in a rat MI model. Hence tyrosol's SIRT1, Akt and eNOS activating power adds another dimension to the white wine research, because it adds a great link to the French paradox. In conclusion these findings suggest that tyrosol induces myocardial protection against ischemia related stress by inducing survival and longevity proteins that may be considered as anti-aging therapy for the heart.[3]

[Athanasios]

There was some discussion in this thread:
http://www.imminst.o...showtopic=38760

I am now taking a supplement that has 1g Vit C and 25mg Olive Extract (OleaSelect) that is offered by Jarrows. I still add a good amount of olive oil to food when possible.

I would still like to hear more discussion on this.

[kismet]

Tyrosol may have the benefits of olive oil. Olive oil has the benefits of olive oil. Isn't this basically enough? Why take something else? (unless of course you are only interested in the mechanism of action for "academic" reasons but I fear that is not the case)

Edited by kismet, 06 June 2010 - 10:11 PM.

[health_nutty]

Tyrosol may have the benefits of olive oil. Olive oil has the benefits of olive oil. Isn't this basically enough? Why something else?

Don't you know that more is better (kidding)

That being said, one can only get so much olive oil in your diet. Is this amount optimal (probably nobody knows the answer to this)? My other concern is that the virgin olive oil I consume is too processed and may not have all the benefits of the olive oil consumed in the Mediterranean (where the studies are being done).

[Blue]

There was some discussion in this thread:
http://www.imminst.o...showtopic=38760

I am now taking a supplement that has 1g Vit C and 25mg Olive Extract (OleaSelect) that is offered by Jarrows. I still add a good amount of olive oil to food when possible.

I would still like to hear more discussion on this.

I would prefer something you can take with every meal because of the effects seen on nf-kb after a single meal from olive oil. Similarly with large amounts of vitamin C, if taking it (which has not gained support in large human studies), then it would seem preferable to take it after every meal due to the very short half-life.

As per the link, I have been considering one or a few sun-dried raw olives (seem to be the only raw ones you can get) or olive leaf extract with every meal instead of or as a complement to olive oil. However, what happens when you sun-dry olives is unclear and olive leaf as noted does not exactly match olive fruit. Still pondering.

Edited by Blue, 06 June 2010 - 10:25 PM.

[health_nutty]

As per the link, I have been considering one or a few sun-dried raw olives (seem to be the only raw ones you can get) or olive leaf extract with every meal instead of or as a complement to olive oil. However, what happens when you sun-dry olives is unclear and olive leaf as noted does not exactly match olive fruit. Still pondering.

I'm not recommending this or doing this myself but here is another option (for now I'm just consuming olive oil):
BAC has a powdered olive leaf extract (I would prefer the fruit). Since it is not capped, you can take a tiny amount of the powder with every meal.

I'm encouraged that olive leaf extact lowers blood pressure (at least it is effective at doing something albeit at a pretty huge dose).
http://www.scienceda...80827002717.htm

[Athanasios]

I am currently taking the Vit C with olive extract about an hour before a meal. I also take other polyphenols with meals and switch up which ones at which meals daily.

General disclaimer:
My risk tolerance used to be lower than most at ImmInst. Even with getting a bit more conservative with supplementation over the last few years, I now seem to be at a higher risk tolerance than most active posters here. I am fine with that and comfortable with my level of supplementation.

[mike250]

how much is too much EVOO? I take one 1-2 tablespoons per day

[Lufega]

I smear olive oil on everything I eat. Everything. I also add a couple of tablespoons of turmeric to my bottle of olive oil and let it settle in. Quite tasty

[Blue]

As per the link, I have been considering one or a few sun-dried raw olives (seem to be the only raw ones you can get) or olive leaf extract with every meal instead of or as a complement to olive oil. However, what happens when you sun-dry olives is unclear and olive leaf as noted does not exactly match olive fruit. Still pondering.

I'm not recommending this or doing this myself but here is another option (for now I'm just consuming olive oil):
BAC has a powdered olive leaf extract (I would prefer the fruit). Since it is not capped, you can take a tiny amount of the powder with every meal.

I'm encouraged that olive leaf extact lowers blood pressure (at least it is effective at doing something albeit at a pretty huge dose).
http://www.scienceda...80827002717.htm

Some of olive leaf extract in fluid form can also be dosed exactly and thus taken with each meal like this one:
http://www.iherb.com...-60-ml/749?at=0

Somewhat interesting that some taking this product claim that it is effective for colds which fit with it being anti-inflammatory. But is this necessarily good? With olive leaf extracts (or the olive fruit pill) you can very easily get tyrosol amounts much higher than the typical olive oil consumer as noted in the earlier link.

The NOW product above gives you 36 mg Oleuropein + unknown amounts of other substances in the suggested dose. As compared to an average consumtion of 2 mg hydroxytyrosol-equivalents in the mediterranean area. Of course, you take less.

The oleaselect tablett contains 30% polyphenols = 45 mg of which a large part most likely is oleuropein which can metabolized to hydroxytyrosol in addition to the hydroxytyrosol already in it. But since it is a tablett maybe you can split it one or two times to reduce the amounts.

Edited by Blue, 07 June 2010 - 01:05 AM.

[Sillewater]

Pharmacol Res. 2010 Apr;61(4):364-70. Epub 2010 Jan 4.
Human absorption of a supplement containing purified hydroxytyrosol, a natural antioxidant from olive oil, and evidence for its transient association with low-density lipoproteins.
González-Santiago M, Fonollá J, Lopez-Huertas E.

Puleva Biotech S.A. 66, Camino de Purchil, Granada 18004, Spain.
Abstract
There is growing interest in the health effects of olive oil polyphenols, particularly hydroxytyrosol (HT), for their potential application in the treatment of inflammatory conditions such as cardiovascular disease (CVD). As oxidative modification of low-density lipoproteins (LDL) plays a central role in the development of CVD, natural antioxidants are a main target for the nutraceutical industry. In this study we firstly investigated the absorption of pure hydroxytyrosol (99.5%) administered as a supplement in an aqueous solution (2.5mg/kg BW) in the plasma and urine of healthy volunteers (n=10). Plasma C(max) for HT and homovanillic alcohol (HvOH) were detected at 13.0+/-1.5 and 16.7+/-2.4min, respectively. The HT and HvOH levels were undetectable 2-h after the administration. HT, HvOH, homovanillic acid and 3,4-dihydroxyphenylacetic acid were found as free forms (44%) or as glucuronide (34.4%) or sulphate (21.2%) conjugates in the 24-h urine samples of the subjects. In a second phase of the study, the same amounts of HT were administered to the subjects and the presence of HT in purified plasma lipoproteins was investigated in LDL fractions freshly isolated. 10min after the ingestion of the HT supplement, more than 50% of the total amount detected was present in the LDL-purified fractions and its concentration declined in accordance with its presence in plasma but no changes were found in total antioxidant capacity, malondialdehyde or LDL lag time. These results indicate that pure HT transiently associates with LDL lipoproteins in vivo. Copyright 2009 Elsevier Ltd. All rights reserved.

I haven't had time to comb through the references but there are some very good papers there regarding bioavailability of the phenols from sources (oil, aqueous, yoghurt).

FASEB J. 2010 Feb 23. [Epub ahead of print]
In vivo nutrigenomic effects of virgin olive oil polyphenols within the frame of the Mediterranean diet: a randomized controlled trial.
Konstantinidou V, Covas MI, Muñoz-Aguayo D, Khymenets O, de la Torre R, Saez G, Del Carmen Tormos M, Toledo E, Marti A, Ruiz-Gutiérrez V, Ruiz Mendez MV, Fito M.

*Cardiovascular Risk and Nutrition Research Group andHuman Pharmacology and Clinical Neurosciences Research Group, Institut Municipal d'Investigació Mèdica (IMIM-Hospital del Mar), Centro de Investigación Biomédica Eu |$$Red (CIBER) de Fisiopatología de la Obesidad y Nutrición, Barcelona, Spain;PhD Program in Biomedicine, Departament de Ciencies Experimentals i de la Salut, Pompeu Fabra University, Barcelona, Spain;Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain;||Department of Preventive Medicine and Public Health and paragraph signDepartment of Nutrition, Food Science, Physiology, and Toxicology, University of Navarra, Navarra, Spain; and#Instituto de Nutrition and Lipid Metabolism, Instituto de la Grasa, Seville, Spain.
Abstract
The aim of the study was to assess whether benefits associated with the traditional Mediterranean diet (TMD) and virgin olive oil (VOO) consumption could be mediated through changes in the expression of atherosclerosis-related genes. A randomized, parallel, controlled clinical trial in healthy volunteers (n=90) aged 20 to 50 yr was performed. Three-month intervention groups were as follows: 1) TMD with VOO (TMD+VOO), 2) TMD with washed virgin olive oil (TMD+WOO), and 3) control with participants' habitual diet. WOO was similar to VOO, but with a lower polyphenol content (55 vs. 328 mg/kg, respectively). TMD consumption decreased plasma oxidative and inflammatory status and the gene expression related with both inflammation [INF-gamma (INFgamma), Rho GTPase-activating protein15 (ARHGAP15), and interleukin-7 receptor (IL7R)] and oxidative stress [adrenergic beta2-receptor (ADRB2) and polymerase (DNA-directed) kappa (POLK)] in peripheral blood mononuclear cells. All effects, with the exception of the decrease in POLK expression, were particularly observed when VOO, rich in polyphenols, was present in the TMD dietary pattern. Our results indicate a significant role of olive oil polyphenols in the down-regulation of proatherogenic genes in the context of a TMD. In addition, the benefits associated with a TMD and olive oil polyphenol consumption on cardiovascular risk can be mediated through nutrigenomic effects.-Konstantinidou, V., Covas, M.-I., Muñoz-Aguayo, D., Khymenets, O., de la Torre, R., Saez, G., del Carmen Tormos, M., Toledo, E., Marti, A., Ruiz-Gutiérrez, V., Ruiz Mendez, M. V., Fito, M. In vivo nutrigenomic effects of virgin olive oil polyphenols within the frame of the Mediterranean diet: a randomized controlled trial.

PMID: 20179144 [PubMed - as supplied by publisher]

J Nutr. 2001 Jul;131(7):1993-6.
The in vivo fate of hydroxytyrosol and tyrosol, antioxidant phenolic constituents of olive oil, after intravenous and oral dosing of labeled compounds to rats.
Tuck KL, Freeman MP, Hayball PJ, Stretch GL, Stupans I.

Centre for Pharmaceutical Research, School of Pharmaceutical, Molecular and Biomedical Sciences, University of South Australia, Adelaide, 5000, Australia. kellie.tuck@unisa.edu.au
Abstract
In vitro studies have shown phenolics in olive oil to be strong radical scavengers. The absorption and elimination of two radiolabeled phenolic constituents of olive oil, hydroxytyrosol and tyrosol were studied in vivo using rats. Compounds were administered intravenously (in saline) and orally (in oil- and water-based solutions). For both compounds, the intravenously and orally administered oil-based dosings resulted in significantly greater elimination of the phenolics in urine within 24 h than the oral, aqueous dosing method. There was no significant difference in the amount of phenolic compounds eliminated in urine between the intravenous dosing method and the oral oil-based dosing method for either tyrosol or hydroxytyrosol. Oral bioavailability estimates of hydroxytyrosol when administered in an olive oil solution and when dosed as an aqueous solution were 99% and 75%, respectively. Oral bioavailability estimates of tyrosol, when orally administered in an olive oil solution and when dosed as an aqueous solution were 98% and 71%, respectively. This is the first study that has used a radiolabeled compound to study the in vivo biological fates of hydroxytyrosol and tyrosol.

PMID: 11435519 [PubMed - indexed for MEDLINE]

Anyways Skot turned me onto tyrosol and oleuperin and I've been doing a bit of reading on it. To me it seems so far the oil based is better absorbed and also olive oil has other benefits beyond tyosol so why supplement with it. I also remember reading that dairy may help with absorption (don't quote me, I'll try and find the study).

Also if anyone has access to this study it would be much appreciated:
Infect Disord Drug Targets. 2009 Aug;9(4):400-14.
Relevance of nutritional antioxidants in metabolic syndrome, ageing and cancer: potential for therapeutic targeting.
Soory M.
PMID: 19689382 [PubMed - indexed for MEDLINE]

[Blue]

Anyways Skot turned me onto tyrosol and oleuperin and I've been doing a bit of reading on it. To me it seems so far the oil based is better absorbed and also olive oil has other benefits beyond tyosol so why supplement with it. I also remember reading that dairy may help with absorption (don't quote me, I'll try and find the study).

One problem with olive oil, as stated in the original thread, is that "The concentration of phenols in extra virgin olive oil varies from 50 to 800 mg/kg". With consumers having no way of determining this. The possible alternatives discussed above was not pure tyrosol. Not sure it if possible to get it at all. If one takes the alternatives with meals with fat, then one may avoid lower bioavailability. Which was not that much lower for rats, "Oral bioavailability estimates of tyrosol, when orally administered in an olive oil solution and when dosed as an aqueous solution were 98% and 71%, respectively."

[lynx]

Life Extension has a number of products with Tyrosol. They are usually out on the bleeding edge.