382 replies to this topic

[hippocampus]

ok, how do I get 60 mcg in 50 mL with 0,3% solution?
isn't 60 mcg the nootropic dose? I would start with that and than take higher doses if needed.

[SuperjackDid_]

Here quote instrustion from Methylene Blue crystals powder

Here step for 0.01% solution

1.Pour 1L of Water
2. Add 0.01g or pinhead MB and stir until it's all blue
3.Solution is ready for use

How do I get 60 mcg from it ?

Edited by Nootropix, 20 January 2012 - 09:28 AM.

[hippocampus]

0,01 mcg in 1L --> 10 mcg in 1 mL --> 60 mcg = 6 mL

[syncleft]

ok, how do I get 60 mcg in 50 mL with 0,3% solution?
isn't 60 mcg the nootropic dose? I would start with that and than take higher doses if needed.

So you want 60 mcg of methylene blue per 50 mL of your final product, correct? Below is using the equation V1C1 = V2C2 to come to your answer. See the bold at the bottom for final answer, though I do suggest you follow along so you know what I did.

Your initial concentration is 0.3%, which is equivalent to 0.3 grams per 100 mL

Converting to milligrams --> (.003 grams/mL) * (1000mg/1g) = 3 mg methylene blue per mL

Volume 1 (how much of your 0.3% solution is necessary, is what we're going to find, so it's unknown)

Concentration 2, which is the concentration we want, is equal to 60mcg/50mL
Converting mcg to mg --> (60mcg/50mL) * (1mg/1000mcg) = .06mg/50mL --> .0012mg/mL

Volume 2 is up to your convenience, I prefer one water bottle amount as that is 500 mL, so I'm going to use that.

Concentration 1 = 3mg/mL
Concentration 2 = .0012mg/mL
Volume 2 = 500 mL

V1C1 = V2C2

Volume 1 = (V2C2)/C1
Volume 1 = (500mL * .0012 mg/mL) / 3mg/mL
Volume 1 = 0.2 mL

Because V2 in the equation is based on the final volume, you would put 0.2 mL of your methylene blue into 500mL - 0.2mL = 499.8 mL of water. (Obviously a negligible amount to reduce from your H20...)

50mL of the final solution will give you 60 mcg of methylene blue.

[mag1]

I am interested in pharmaceutical grade Methylene Blue. Anyone have a suggestion of a supplier that could ship MB to Canada?
I have read that MB is available over the counter in Canada. Does anyone know of a seller in Toronto that sells OTC pharmaceutical grade MB?

[elovefire]

Ok so I just read thru the whole thread.
I have kordon MB 2.3%.
-I took 1ml MB and put it in 19ml h2o.
-Then, took 1ml of that solution and put it in (diluted) 18ml.
-then I took 1 ml of that last solution and put it in a glass of water and drank it.
That technique came from devinthayer. Ty, DT.

So did I do that right to get 60mcg?
Is that the best dose to take?
Just double checking to get the best results.

[Dan009]

Hey guys,

Got a bottle from a local aquarium supplies store (Canberra, Australia).

It contains 12mg/ml - hoping you guys can check my maths is correct...



First dilution:

5ml of the prodct (60mg/60000mcg of MB) with 100ml water.

So 100ml = 600mcg per ml or 6000mcg per 10ml (might be easier to measure).

Second Dilution:

Diluted 10ml from the first step in to 100ml of water, so now 100ml = 6000mcg or 600mcg per 10ml or 60mcg per ml.

Final Dilution:

Took 10ml from the pvrevious step and diluted it into 1000ml (1L) for water, so now 100ml = 60mcg (target dose to start with) ? Color is a very light blue.

Anyone see any errors with this? When I try higher doses, will make the final step more concentrated.

Cheers.

[Googoltarian]

First dilution:

5ml of the prodct (60mg/60000mcg of MB) with 100ml water. <-this gives 105ml

So 100ml = 600mcg per ml or 6000mcg per 10ml (might be easier to measure). <- so you get 0,57mg/ml

[Dan009]

First dilution:

5ml of the prodct (60mg/60000mcg of MB) with 100ml water. <-this gives 105ml

So 100ml = 600mcg per ml or 6000mcg per 10ml (might be easier to measure). <- so you get 0,57mg/ml

Sorry, should have worded it better, The total for the dilutions what the amounts I specified, ie I should have said, added 95ml of water to make 100ml.

Other than that, are my calculation correct? Is 100ml of the final solution going to yield 60mcg of MB?

Thanks Googoltarian

[niner]

Other than that, are my calculation correct? Is 100ml of the final solution going to yield 60mcg of MB?

The math looks fine. Whether or not 60mcg is going to do anything beyond a placebo effect is an entirely different question.

[maxwatt]

True, many have found doses of 0.5 mg to 1 milligram to be much more noticeable.

[qemist]

Delete this link if inappropriate, but I notice a highly rated seller has what looks like Korean manufactured laboratory Methylene Blue trihydrate at $31 for 25g. This should be close to a life-time supply even for the 1mg p.d. people, let alone the 60 mcgers. Would there be any safety concerns with this form of MB?

[jayfoxpox]

Hi all,


http://www.ncbi.nlm....pubmed/17428524
Rats take 1mg/kg of MB a day ( if I read it right)

According to the methods described here
http://www.longecity...animal-studies/
so since the dosage for rats is 1mg/kg

1(mg/kg) x 6km(rat)/37km(human = 0.16mg/kg
I’m 97 kg and the formula is based on 60 kg , so there’s likely going to be some error here . But I’ll go on anyways.
0.16mg/kg x 97 kg = 15.52 mg

Would that mean I should take 5 mg dosages 3 times a day?

And also , did anyone find any papers on the effects it has from long term usages?
Though these are low dosages it has me worried what effects will creep up from using it regularly for a year or 5.
Has anyone found papers on rats with a dosage lower than 1mg/kg?

Edited by jayfoxpox, 29 July 2012 - 04:36 PM.

[rashlan]

Hey, is everyone taking their methylene blue on an empty stomach or with food? The reason I ask is that in the rember trials the

100-mg dose, had "absolutely no activity because it didn't dissolve in the stomach."

Would a 60 milligram dose in an elderly

patient

be more likely to be effective on an empty stomach or after food?

[rashlan]

Also is anyone experiencing oily skin since starting Methylene Blue?

[Turnbuckle]

Hey, is everyone taking their methylene blue on an empty stomach or with food? The reason I ask is that in the rember trials the

100-mg dose, had "absolutely no activity because it didn't dissolve in the stomach."

This explanation doesn't wash. Why wouldn't it dissolve in the stomach when the other doses did dissolve? And why would dissolving defective tau help when, once the MB was stopped, it would crystallize right back into defective microtubules? So you would have to take MB forever for AD, and if you took too much (as perhaps happens with 100 mg) you might experience excessive breakdown of the microtubules.

So I think the hyperphosphorylation at the root of the problem needs to be addressed simultaneously. Such as with sodium selenate, nicotinamide, and/or propranolol.

In the present study we tested whether propranolol, a β-receptor antagonist commonly used as antihypertensive drug, could ameliorate the cognitive impairments and increases in AD-related markers shown by the senescence-accelerated mouse prone-8 (SAMP8). Propranolol administration (5 mg/kg for 3 weeks) to 6-month-old SAMP8 mice attenuated cognitive memory impairments shown by these mice in the novel object recognition test. In the hippocampus of SAMP8 mice it has been found increases in Aβ(42) levels, the principal constituent of amyloid plaques observed in AD, accompanied by both an increased expression of the cleaving enzyme BACE1 and a decreased expression of the degrading enzyme IDE. All these effects were reversed by propranolol treatment.

http://www.ncbi.nlm....pubmed/22824191

These preclinical findings suggest that oral nicotinamide may represent a safe treatment for AD and other tauopathies, and that phosphorylation of tau at Thr231 may regulate tau stability.

http://www.ncbi.nlm....pubmed/18987186

As hyperphosphorylation of tau precedes NFT formation, the effect of selenate on tau phosphorylation was assessed in more detail, a process regulated by both kinases and phosphatases....Taken together, sodium selenate mitigates tau pathology in several AD models, making it a promising lead compound for tau-targeted treatments of AD and related dementias.

http://www.ncbi.nlm....erapy Alzheimer

Edited by Turnbuckle, 08 August 2012 - 10:07 AM.

[niner]

http://www.ncbi.nlm....pubmed/17428524
Rats take 1mg/kg of MB a day ( if I read it right)

According to the methods described here
http://www.longecity...animal-studies/
so since the dosage for rats is 1mg/kg

1(mg/kg) x 6km(rat)/37km(human = 0.16mg/kg
I’m 97 kg and the formula is based on 60 kg , so there’s likely going to be some error here . But I’ll go on anyways.
0.16mg/kg x 97 kg = 15.52 mg

But those rats were injected, not dosed orally, so you will need more. Atamna was using something on the order of 60mg orally in humans, if I recall correctly. That's the dose used in the Rember trials, too. I think that's about what it would take to replicate the rat study.

Hey, is everyone taking their methylene blue on an empty stomach or with food? The reason I ask is that in the rember trials the 100-mg dose, had "absolutely no activity because it didn't dissolve in the stomach."

Would a 60 milligram dose in an elderly patient be more likely to be effective on an empty stomach or after food?

Like Turnbuckle, I don't buy this business of 100mg "not dissolving". MB is soluble as hell. Was that some sort of weird formulation or something? I'd be inclined to take it on an empty stomach, but I'm not sure it would matter.

And why would dissolving defective tau help when, once the MB was stopped, it would crystallize right back into defective microtubules? So you would have to take MB forever for AD, and if you took too much (as perhaps happens with 100 mg) you might experience excessive breakdown of the microtubules.

Dissolving tau aggregates might allow the tau monomers to be cleared, and once they're gone, they're gone. Even if you needed to take MB forever, that's a hell of a lot better than AD. It's cheap and is a pretty benign compound, all in all.

[Turnbuckle]

Dissolving tau aggregates might allow the tau monomers to be cleared, and once they're gone, they're gone. Even if you needed to take MB forever, that's a hell of a lot better than AD. It's cheap and is a pretty benign compound, all in all.

Might is the operative word. If they aren't cleared, then converting them to their normal state would make sense, especially if such cheap and well known supplements and drugs will do it. And after that, MB would no longer be necessary.

Edited by Turnbuckle, 08 August 2012 - 06:53 PM.

[ClarkSims]

I take MB and have for about half a year.

Geddarkstorm, how much MB do you take, and where do you get it?

(assuming the standard human has 5 liters of blood).

The drug is water soluble, so it should diffuse through the entire water volume of the human body. Assuming the body is about 60 percent water, and the person weighs about 80 kg, that is about 45 KG of water, as opposed the the 5 Liters, or kg in your calculation.

Shouldn't you use the entire water volume, and not the blood volume?
This would mean that you would need to take about 10 mg per day, as opposed to 1 mg per day.
10 mg per day, is about 1/3 of the low dose group in the TauRX study, which used 30 mg and 60 mg doses.

[ClarkSims]

I take MB and have for about half a year.

Geddarkstorm, how much MB do you take, and where do you get it?

(assuming the standard human has 5 liters of blood). We want to keep MB around 100 nM in the blood as long as possible, so by taking around 300 or 500 nM, since we have to be aware of absorption and excretion kinetics, that should keep levels around 100 nM for as long as possible without spiking up towards 1 uM where MB's mitochondrial effects are halved. This also should allow proper dosage of MB to filter into peripheral tissues.

Methylene Blue will diffuse through the entire water volume of the body wont it? So that would be closer to 40 liters of liquid, wouldn't it?

I get that by assuming the person is about 70 Kg, and about 60% water,
70 * .6 = 42 kg = 42 liters

[ClarkSims]

Here are 2 links for what is advertised as "UltraPure Grade":

http://www.bioexpres...200212000212260


http://search.anaspe...=methylene blue (Will have to select country and redo the search)


Those came from the comments that follow this article:

http://pipeline.cora...es_comeback.php

Note comment #61 geddarkstorm

I took a look at the bioexpress site, and I couldn't find what was the concentration of their methylene blue.

Does anyone know what the concentration is?

[sbidl1]

Bump! Newbie here. Been lurking for a while. Could anyone help advise me on how to get to a 60mcg dose from a 12mg/ml methylene blue solution? Also... Sorry for being fussy, but is it possible to concentrate this so I'm not having to drink 500ml of water every time I want to take some MB. Assuming I will be usin 60mcg 3 times daily.

[highchief]

There's two things that I'm a bit unclear about:

I've read most of the big threads and all of the 'Experiences' thread and if I remember correctly, it was mentioned that it might take 2 weeks to get noticeable effects.
At the same time, the recommended starting dose was 60mcg, and there was some debate on the accuracy of that.
So my question is, should I stick to 60mcg doses for the first two weeks and then start changing the dose if I'm not experiencing effects? Or should I start with the 60mcg and titrate on a daily basis to find the optimal dose?

I started at 60mcg 3 times a day 3 days ago, and I'm at 90mcg 4 times a day now.
For what it's worth, I'm 6'5", 215 lbs, my urine hasn't changed color.

[Charmion]

What is the difference between R-250 methylene blue http://www.anaspec.c...ct.asp?id=29114
and G-250 methylene blue http://www.anaspec.c...ct.asp?id=29113
?

[rwac]

Both are blue dyes but neither of them are Methylene Blue.

[penisbreath]

I apologize if this has been covered already, but the methylene blue I obtained from the store for use in fish tanks has a warning not to ingest .. there's no ingredients listed, and the product's simply called 'methylene blue'.

Is this just a general/obligatory warning that I should ignore?

[maxwatt]

Probably. Fish are pretty sensitive and any nasties would likely kill them. But the label should give you the concentration of the solution of MB.

[Charmion]

Thats strange. Then does anyone know which of these search results are supposed to be the methylene blue? http://search.anaspe...=methylene blue

[pleb]

I'm no Chemist but looking at the link i don't think any off them are methylene blue, MB is an aniline dye,invented in the 1800's
you can get it from almost any shop that sells tropical fish,and used to kill fungus and other diseases,,

it used to be known as dolly blue in the 1940's and 50's
and used by women to dye parachute silk to make scarves, blouses, etc from surplus,stock after WW11

( showing my age now ! ),

Edited by pleb, 04 April 2013 - 07:28 AM.

[penisbreath]

Thats strange. Then does anyone know which of these search results are supposed to be the methylene blue? http://search.anaspe...=methylene blue

Looks like it's 12mg/ml of methylene blue ..