75 replies to this topic

[CAtransplant]

I've had several years of very positive results.

Soak astraglaus root (1 lb) in 1500 ml of Everclear for 2 months before straining it. Drink 1 oz of the potent tincture every other night before bed (Mon, Wed, Fri, Sun). Skip a week, then resume.

Save supps like fish oil, garlic, resveratrol, reishi, he shou wu, or anything else that may impede or destroy telemorase for morning, but it is very important to take them. The telemorase should be destroyed the next morning every time, reishi works well. This is just what I do folks.

 

 

Please clarify re: need to destroy telemorase next morning. Are you saying telemorase cannot be taken and the body allowed to use what it needs or expel what it doesn't? You HAVE to take one or more of the noted supplements or it is not advisable to take telemorase in the manner you indicate (or at all)? If so, does your comment about reishi working well mean that taking just that supp will suffice to destroy telemorase, or is it necessary to take one of more of the others as well? Thanks.

[1kgcoffee]

There's this unproven idea floating around that excessive activation of telomerase could lead to the development of cancer.

I have begun taking a similar tincture (half astragalus, half other herbs) and it does seem to be having a subjectively positive effect.

[jamesagreen]

Alphabetical Index for Telomerase Activators under Investigation [> Telomerase Inhibitors]

2OR,25S-epoxy-3β,16β,25-trihydroxy-9β-methyl-19-norlanost-1,5-diene (6f).
5-azacytidine (22). (DANGER: may promote prostate carcinomas).
26-deoxyactein (key Black Cohosh (18) extract saponin)
Acacia Bark Extract (133) (Product B, List) lengthens fibroblast telomeres.
Acetyl L-Carnitine (via NGF) (109).
Akt protein kinase (58), phosphorylates hTERT, upregulated by resveratrol & Sphingosine-1-Phosphate.
Amphiregulin (112), EGF family member, via MAP Kinase pathway, candidate.
Androgens can activate telomerase, perhaps after conversion into estrogens (64) [Forskolin].
Angiotensin II (82).
Anti-CD3 monoclonal antibody (mAb) (50).
Antigenic stimulation activates telomerase in T-cells (48).
Antisense oligode-oxyribonucleotides (ODNs) against Bcr-Abl/c-Abl mRNA (61).
Anthraquinone, New Symmetrical Bis-Substituted Derivatives of, (98).
AP-1 transcription factor activator protein (125).
AP-2 (Activating Enhancer-binding Protein-2) (89).
Arginine (130), by upregulating Nitric Oxide and HGH.
Arsenic, a poisonous tumor promoter, also elevates hTERT levels (63) Inhibits telomerase.
Asian Ginseng Root Extract (135) (Product B, List) (Panax Ginseng) lengthens fibroblast telomeres.
Astragalus Root (6_) [Index],
Astragalus Root Extract (6_) [Index],
Astragaloside IV (6c).
Astragaloside IV 16-one (6e).
Astragenol (6e).
Astraverrucins (126).
Bacopa (124). (136) Bacopa Extract (Product B, List) lengthens fibrobast telomeres.
Bcl-2 (75).
Ben Gurion University on the Negev, (99) Novel Telomerase Activators developed at.
Berberine Rhizome Extract (137) (Coptis Chinensis) (Product B, List) lengthens fibrobast telomeres.
Betacellulin (114), EGF family member, via MAP Kinase pathway, candidate.
Beta ecdysterone (20-hydroxyecdysone) (54). [Not known to be telomerase activator.]
Bisphenol A (BPA) (11).
Black Cohosh (18). C. racemosa (black cohosh) extracts (26-deoxyacetein) and other Cimicifuga family extracts.
Black Tea Extract (138) (Camellia sinensis) (Product B, List) lengthens fibrobast telomeres.
Bmi-1 protein (66). (Dangerously associated with the breast cancer Bmi-1 oncogene.)
Boswellia Fruit Extract (139) (Boswellia serrata) (Product B, List) lengthens fibrobast telomeres.
C3-(L)-isoleucyl-cycloastragenol (100.4), the most enhanced-bioavailability Geron telomerase activator.
4 C3-(L)-valyl-cycloastragenol (100.3), experimental Geron telomerase activator (Nov 18, 2010).
C-Abl tyrosine kinase (91), phosphorylates hTERT.
Ca ionophore (51) .
Carnosic Acid promotes Nerve Growth Factor to activate Id-1 helix-loop-helix protein transcription (73).
Cimicifuga (18). C. racemosa (black cohosh) extracts (26-deoxyacetein) and other Cimicifuga family extracts.
Citrulline (131), by upregulating Nitric Oxide and HGH.
C0057684 (37), (47) from Sierra Sciences.
C0313741 (37b) from Sierra Sciences.
CD40 [or Surface Ig (31)] activates telomerase in memory B-cells.
CGK733 (23). Revives senescent cells, but not a true telomerase activator.
CGK 1026 (19). CGK 1026 derepresses hTERT expression. Used in therapy to replace Tricostatin A.
c-Myc (59) is upregulated by EGF, Colostrum, and PDGF.
Cocoa (44). [Not known to be a telomerase activator.]
Colostrum (68) (Contains EGF (30)).
Cottage Cheese (casein) (110) by upregulating IGF-1 [Index].
Cycloastragenol (6d). Same as Geron TAT2, or TAT0002, the primary TA-65 ingredient.
Cycloastragenol 3-β-D-xylopyranoside (6h).
Cycloastragenol 6-β-D-glucopyranoside (6g).
DHEA (Dihydroepiandrosterone) (71).
Diosgenin promotes HIF-1 (Hypoxia-inducible factor 1) (24).
DL-Alpha Lipoic Acid (134) (Product B, List) lengthens fibroblast telomeres. Antioxidant.
DMSO (Dimethyl Sulfoxide) (29). [Telomerase Inhibitors/Dimethyl Sulfoxide (DMSO) (46)].
Doxycyclin (35b). Presence or absence of telomere growth can depend on the presence of doxycyclin.
E1A (95) upregulates hTERT transcription by binding to Sp1 sites.
E6/E6AP viral protein, a ubiquitin ligase from human papilloma virus (65). Poisonous.
Ecdysone (16). An insect hormone.
Enhanced-bioavailability telomerase activators (100) from Geron in 2010.
Ependymin Peptide (26). From goldfish brains: sequence ESCKKETLQFRKHL.
Epidermal growth factor EGF (30).
Epithelial Growth Factor (EGF) (83)
Epiregulin (107), an Epidermal Growth Factor family member.
Epithalon peptide (1) (Ala-Glu-Asp-Gly). Injectable. Discovered in 2003.
ER81 Transcription Factor (90).
EST1, for yeast (7).
EST1p, for yeast under (7) including its homologue hEST1A in humans and yeast EST1.
Estrogen (10).
Estrogen Receptors (13–18) - entry (10b)
Ets transcription factor (96) upregulates hTERT transcription.
Exercise (81).
FBX4 (117) overexpression lengthens telomeres.
Fenugreek Seeds & Fenugreek Extract (105) via Tankyrase, HIF-1 and Diosgenin.
FGF-1 (88).
FGF-2 (53).
FGF-7 (88).
Fibroblast Growth Factor (FGF) (88): FGF-1, FGF-2, FGF-7 and other FGF growth factors.
Fibroblast Growth Factor 2 (FGF-2) (53).
Folic acid (106), by phosphorylating cytoplasmic hTERT with AKT.
Forskolin [Index] (Androgens (64), Testosterone) for muscles, heart cells.
FR901228 (39). (Romidepsin) Japanese research at Okayama University.
Gamma Tocotrienol (123). A Terraternal report.
Ginkgo Biloba (86), by promoting the expression of HIF-1. Also taken with astragalus extract to improve circulation.
Ginsenoside Rg1 (45).
Ginsenoside RH1 (6e).
Glucocorticoid "putative" sites (97) upregulate hTERT transcription.
? Gotu Kola ? (69). Taken with astragalus extract to improve circulation.
G-CSF: Granulocyte Colony-Stimulating Factor (56).
Grape Seed Extract (140) (Vitis vinifera) (Product B, List) lengthens fibrobast telomeres.
Green Tea Extract (141) (Camellia sinensis) (Product B, List) lengthens fibrobast telomeres.
GRN139951 (Geron) (5). (Probably astragaloside IV.)
GRN510 (122)- A new Geron telomerase activator based on a Chinese medicinal herb.
hALP - Histone acetyltransferase hALP, activates hTERT transcription (93).
Harada Fruit Extract (142) (Terminalia Chebula) (Product B, List) lengthens fibrobast telomeres.
Haritaki (Terminalia chebula, Combretaceae), ethanol extract. (67) (Antioxidants.)
Hawthorn Fruit Extract (143) (Crataegus pinnatifida) (Product B, List) lengthens fibrobast telomeres.
Heat shock proteins (20). Heat shock proteins like Hsp90 mediate telomerase assembly.
hEST1A,the human homologue of yeast EST1p (See also (76)), in (7) with EST1 for yeast.
Hepatocyte Growth Factor (78).
Heregulins (115), EGF family member, via MAP Kinase pathway, candidate.
HP-EGF (113) (Heparin-binding EGF), EGF family member, via MAP Kinase pathway, candidate.
HIF-1 (24). Hypoxia-inducible factor 1 (HIF-1).
Histone deacetylase inhibitor possibilities (12). Other HDAC inhibitor possibilities.
HGH, Human Growth Hormone (8).
Horny Goat Weed (144) (Epimedium saggittatum) (Product B, List) lengthens fibrobast telomeres.
hRAP1 (36).
Id-1 helix-loop-helix protein (73), upregulated by Nerve Growth Factor.
IGF-1 (9).
IGF-2 (111), by upregulating progesterone [Index].
IL-2, Interleukin 2 (3). Injectable.
IL-3 (94) upregulates telomerase in hematopoietic stem cell in vitro cultures.
IL-7, Interleukin-7 (32), appears to stimulateS expression of telomerase within T-cells.
IL-15, Interleukin-15 (33) stimulates telomerase in T-cells.
Interleukin 2 (3).
IRF4 - Interferon Regulatory Factor 4 (and to a lesser degree IRF8), (70).
Juve Tea (49).
Lithium (128), by upregulating Bcl-2 (75).
Lysophosphatic Acid (80).
Maca Root Extract (145) (Lepidium Meyenii) (Product B, List) lengthens fibrobast telomeres.
Milk Thistle Extract (146) (Product B, List) lengthens fibrobast telomeres.
Mre11 (25). Mre11 protein.
NAC (N-acetyl-cysteine) (127) activates telomerase in endothelial cells.
Nerve Growth Factor activates Id-1 (73). Rosemary, Carnosic Acid, Acetyl L-Carnitine, Huperzine A up NGF.
Resveratrol (58b). Phosphorylates hTERT via Akt Protein Kinase.
NF-kappaB (40). [Transcription factor, for inflammatory cytokines.]
Nicotine (85).
Nitric Oxide (21).
Novel Telomerase ActivatorS developed at Ben Gurion University on the Negev. (46)
Nucleostemin (118) lengthens telomeres by degrading TRF1.
Okadaic acid (34).
Omega-6 < Omega-3 balance (132) can lengthen telomeres in leukocytes.
Plantain Leaf Extract (147), (Product B, List) lengthens fibrobast telomeres.
Platelet Derived Growth Factor (PDGF) (38).
Phorbol 12, 13-dibutyrate (52).
Pomegranate Fruit Extract (148) (Punica granatum) (Product B, List) lengthens fibrobast telomeres.
Product B (121) from IsAGenix.
Protein Phosphatase 2A (92), phosphorylates hTERT. Telomerase Inhibitor.
Portulaca Oleracea (Purslane) (87). (Antioxidant.)
PhosphatidylInositol 3'-kinase (57).
Pregnenolone. (72)
Progesterone (60)
Protein kinase C (27).
Purslane (Portulaca Oleracea) (87). (Antioxidant.)
Raloxifine (15). (orally bioavailable Evista from Eli Lilly).
Replication Protein A (76) might be upregulated by Sodium Butyrate or Sodium 4-Phenylbutyrate (check).
Rapamycin (116) rejuvenates progeria cells, extends mouse lifetimes.
Resveratrol (150) (Product B, List) lengthens fibrobast telomeres.
Resveratrol (84) (See also (58) Akt with (58b) Resveratrol).
Retinoic acid (28). [Telomerase Inhibitors/Retinoic Acid (27)]. Limits p16INK4A.
Rosemary Extract promotes Nerve Growth Factor to activate Id-1 (73).
Saquinavir (17). (Invirase [Roche Invirase]) an anti-HIV drug and protease inhibitor.
?Shark Liver Oil? (42) [Not known to be a telomerase activator.]
Silver (Ag) (101) upregulates telomerase in immune system cells.
Silymarin (102) activates telomerase.
Simvastatin upregulates Bcl-2 (75), a telomerase activator.
Sodium Butyrate (103) upregulates Sp1, which upregulates hTERT.
Sp1 (43) is upregulated by sodium butyrate, and down-regulated by low insulin.
Sphingosine-1-Phosphate (S1P). (79)
STAT3 (14).
Stem Cell Factor (Kitl) - (74).
Surface Ig (31).
Survivin (62).
TA-65 (TA Sciences) (6b). (Formerly GRN-665, Probably cycloastragenol, or Geron TAT0002.)
TAC1 with auxin (13).
Tankyrase (35). A telomeric PARP phosphorylated by insulin and promoted by Niacinamide.
TAT2 (Geron) (4). (TAT2 is cycloastragenol, the common alglycone of the astragalosides).
TAT0002 (Geron) (4). (TAT0002 is cycloastragenol, the common alglycone of the astragalosides).
TAT153 (119), announced by Geron in 2010.
Terminalia chebula (Combretaceae), the ethanol extract (67). (Antioxidant.)
TGF-alpha (108), (Transforming Growth FActor Alpha) - candidate telomerase activator.
TNF-alpha (41). [Inflammatory]
Trapoxin (104) is an HDAC inhibitor like Tricostatin A, may upregulate hTERT.
Tricostatin A (2). Injectable. Discovered as a telomerase activator in 2000.
Tri-Phenyl Compound Telomerase Activators (99) developed at Ben Gurion University.
Turmeric Root Extract (151) (Curcuma longa) (Product B, List) lengthens fibrobast telomeres.
UP1 (120) Works in UB1=UAGGGU-expressing cells, may be applied exogenously.
Uncaria sinensis Havil (77) Retards telomere shortening.
VEGF2 & VEGFA (55), Vascular Endothelial Growth Factor (VEGF).
Velvet Bean Extract (152) (Mucuna Pruriens) (Product B, List) lengthens fibrobast telomeres.
White Tea Extract (153) (Camellia sinensis) (Product B, List) lengthens fibrobast telomeres.
Wild Yam, via Diosgenin promotion of HIF-1 expression activating hTERT transcription.
Zinc (129), by upregulating Bcl-2 (75).

The discovery dates associated with these substances under investigation are in the non-alphabetical list:

List Pointers for Telomerase Activators under Investigation [> Telomerase Inhibitors]

(1) Epithalon peptide, (Ala-Glu-Asp-Gly). Injectable. Discovered in 2003. 2003
(2) Tricostatin A. Injectable. Discovered as telomerase activator in 2000. 2000
(3) Interleukin 2. Injectable 2005
(4) TAT0002 (Geron). (TAT2 = cycloastragenol, Geron 2005, main TA-65 ingredient). 2005
(5) GRN139951 (Geron). (Probably astragaloside IV.) 2005
(6) GRN140665 (Geron). (Mostly cycloastragenol, or Geron TAT0002 or TA-65). 2005
__(6b) TA-65 (TA Sciences). (GRN-665, mostly cycloastragenol, or Geron TAT0002). 2007
__(6_) Astragalus Root [Index], 2005
__ (6_)Astragalus Root Extract [Index], 2005
__(6c) astragaloside IV. Orally bioavailable, announced in 2005. 2005
__(6d) cycloastragenol. Orally biovailable, TAT2, the primary TA-65 ingredient. 2005
__(6e) astragenol. Orally bioavilable, announced in 2005. 2005
__(6e) astragaloside IV 16-one. Orally bioavailable, announced in 2005. 2005
__(6f) 2OR,25S-epoxy-3β,16β,25-trihydroxy-9β-methyl-19-norlanost-1,5-diene 2005
__(6g) cycloastragenol 6-β-D-glucopyranoside 2005
__(6h) cycloastragenol 3-β-D-xylopyranoside 2005
__(6e) Ginsenoside RH1. Orally bioavailable. Announced in 2005. 2005
(7) EST1, for yeast, including EST1p and its homologue hEST1A in humans 2003
(8) HGH, Human Growth Hormone. 2005
(9) IGF-1. 2001
(10) Estrogen. 1999
(10b) Estrogen Receptors (13–18). 1999
(11) Bisphenol A (BPA). 2004
(12) Other histone deacetylase inhibitor possibilities. .
(13) TAC1 with auxin. 2003
(14) STAT3. 2005
(15) Raloxifine. (orally bioavailable Evista from Eli Lilly). 2006
(16) Ecdysone. An insect hormone. 2006
(17) Saquinavir. (Invirase [Roche Invirase]) an anti-HIV drug and protease inhibitor. 2001
(18) Cimicifuga Extracts. C. racemosa (black cohosh) extracts (26-deoxyacetein). 2005
(18b) 26-deoxyacetin a C. racemosa (black cohosh) extract. 2005
(19) CGK 1026. CGK 1026 derepresses hTERT expression. 2004
(20) Heat shock proteins. Heat shock proteins like Hsp90 mediate telomerase assembly. 1999
(21) Nitric Oxide. (Vasa, 2000, Hayashi 2007) 2000
(22) 5-azacytidine. (DANGER: may promote prostate carcinomas). 2002
(23) CGK733. Revives senescent cells, but not a true telomerase activator. October 19, 2007
(24) HIF-1. Hypoxia-inducible factor 1, promoted by diosgenin, ginkgo biloba, and exercise. 2006
(25) Mre11. Mre11 protein. 2002
(26) Ependymin Peptide. From goldfish brains: sequence ESCKKETLQFRKHL. 2005
(27) Protein kinase C. 2002
(28) Retinoic acid. [Telomerase Inhibitors/Retinoic Acid]. Limits p16INK4A. 2000
(29) DMSO (Dimethyl Sulfoxide). [Telomerase Inhibitors/Dimethyl Sulfoxide (DMSO)].
(30) Epidermal growth factor EGF. 2002
(31) Surface Ig. 1997
(32) IL-7, Interleukin-7, appears to stimulate expression of telomerase within T-cells. 1998
(33) IL-15, Interleukin-15 stimulates telomerase in T-cells. 2005
(34) Okadaic acid. 2002
(35) Tankyrase. A telomeric PARP phosphorylated by insulin and promoted by Niacinamide. 2004
(35b) Doxycyclin. Telomere growth can depend on the presence of doxycyclin. .
(36) hRAP1. 2004
(37) C0057684 from Sierra Sciences. 2007
(37b) C0313741 from Sierra Sciences (announced February 2010, noted here Feb 2013). 2010
(38) Platelet Derived Growth Factor (PDGF). 2004
(39) FR901228. (Romidepsin) Japanese research at Okayama University. 1998
(40) NF-kappaB. [Transcription factor, for inflammatory cytokines.] 2000
(41) TNF-alpha. [Inflammatory] 2004
(42) ?Shark Liver Oil? [Not known to be a telomerase activator.]
(43) Sp1 is upregulated by sodium butyrate, and down-regulated by low insulin. 2000
(44) Cocoa. [Not known to be a telomerase activator.]
(45) Ginsenoside Rg1. 2011
(46) Novel Telomerase ActivatorS developed at Ben Gurion University on the Negev. 2008
(47) C0057684. From Sierra Sciences. 2002
(48) Antigenic stimulation activates telomerase in T-cells. 2002
(49) Juve Tea. 2007
(50) Anti-CD3 monoclonal antibody (mAb). 1996
(51) Ca ionophore. 1996
(52) Phorbol 12, 13-dibutyrate. 1996
(53) FGF-2, Fibroblast Growth Factor 2 . 2003
(54) beta ecdysterone (20-hydroxyecdysone). [Not known to be telomerase activator.]
(55) VEGF2 & VEGFA. 2009
(56) G-CSF: granulocyte colony-stimulating factor. 2003
(57) PhosphatidylInositol 3'-kinase. 2005
(58) Akt protein kinase, phosphorylates hTERT. 1999
(58b) Resveratrol. Phosphorylates hTERT via (58) Akt Protein Kinase. See also (84) 2009
(59) c-Myc is upregulated by EGF, Colostrum, and PDGF. 1999
(60) Progesterone. 2002
(61) Antisense oligode-oxyribonucleotides (ODNs) against Bcr-Abl/c-Abl mRNA. 2004
(62) Survivin. 2005
(63) Arsenic, a poisonous tumor promoter, also elevates hTERT levels. Inhibits telomerase.
(64) Androgens can activate telomerase, perhaps after conversion into estrogens [Forskolin]. 2003
(64b) Forskolin [Index], for the heart, muscles, and melanocytes. 2003
(65) E6/E6AP viral protein, a ubiquitin ligase from human papilloma virus. Poisonous.
(66) Bmi-1 protein (66). (Dangerously associated with the breast cancer Bmi-1 oncogene.) 2002
(67) Terminalia chebula (Combretaceae, Haritaki), ethanol extract. (Antioxidant.) 2012
(68) Colostrum (Contains EGF (30)). 2002
(69) ? Gotu Kola ?. Taken with astragalus extract to improve circulation.
(70) IRF4 - Interferon Regulatory Factor 4 (and to a lesser degree IRF8). 2009
(71) DHEA (Dihydroepiandrosterone). 2003
(72) Pregnenolone. 2003
(73) Id-1 helix-loop-helix protein, upregulated by Nerve Growth Factor. 1999
(74) Stem Cell Factor (Kitl). 2002
(75) Bcl-2. 1997
(76) Replication Protein A. 2003
(77) Uncaria sinensis Havil Just retards telomere shortening. 2006
(78) Hepatocyte Growth Factor. 2005
(79) Sphingosine-1-Phosphate (S1P). 2007
(80) Lysophosphatic Acid. 2007
(81) Exercise. 2001
(82) Angiotensin II. 2006
(83) Epithelial Growth Factor (EGF). 2002
(84) Resveratrol. (See also (58) Akt with (58b) Resveratrol). 2009
(85) Nicotine. 2009
(86) Ginkgo Biloba, by promoting the expression of HIF-1. 2007
(87) Purslane (Portulaca Oleracea). (Antioxidant). 2007
(88) Fibroblast Growth Factor (FGF): FGF-1, FGF-2, FGF-7, other FGF growth factors. 2003
(89) AP-2 (Activating Enhancer-binding Protein-2). 2007
(90) ER81 Transcription Factor. 2004
(91) C-Abl tyrosine kinase, phosphorylates hTERT. 2002
(92) Protein Phosphatase 2A, phosphorylates hTERT. Telomerase Inhibitor.
(93) hALP - Histone acetyltransferase hALP, activates hTERT transcription. December 2010
(94) IL-3 upregulates telomerase in hematopoietic stem cell in vitro cultures. January 2011.
(95) E1A upregulates hTERT transcription by binding to Sp1 sites. 2007
(96) Ets transcription factor upregulates hTERT transcription. 2007
(97) Glucocorticoid "putative" sites upregulate hTERT transcription. 2007
(98) New Symmetrical Bis-Substituted Derivatives of the Anthraquinone. 2003
(99) Tri-Phenyl Compound Telomerase Activators developed at Ben Gurion University. 2008
(100) Enhanced-bioavailability telomerase activators from Geron (2010). 2010
(101) Silver (Ag) upregulates telomerase in immune system cells. 1997
(102) Silymarin activates telomerase. 2010
(103) Sodium Butyrate upregulates Sp1, which upregulates hTERT.
(104) Trapoxin HDAC inhibitor like Tricostatin A, may upregulate hTERT.
(105) Fenugreek Seeds & Fenugreek Extract, via Tankyrase, HIF-1 and Diosgenin. 2004, 2006
(106) Folic acid, by phosphorylating cytoplasmic hTERT with AKT. 2009
(107) Epiregulin, an Epidermal Growth Factor family member. 2003
(108) TGF-alpha, (Transforming Growth Factor Alpha) - candidate telomerase activator. 2003
(109) Acetyl L-Carnitine (via NGF production of Id-1). 2002
(110) Cottage Cheese (casein) by upregulating IGF-1 [Index].
(111) IGF-2, by upregulating progesterone [Index]. 2002
(112) Amphiregulin, EGF family member, via MAP Kinase pathway, candidate. October 27, 2011
(113) HP-EGF (Heparin-binding EGF), EGF family member, via MAP Kinase pathway, candidate. 2002
(114) Betacellulin, EGF family, via MAP Kinase pathway. 2002
(115) Heregulins, EGF family, via MAP Kinase pathway. 2002
(116) Rapamycin rejuvenates progeria cells, extends mouse lifetimes.
(117) FBX4 overexpression lengthens telomeres. 2006
(118) Nucleostemin lengthens telomeres by degrading TRF1. 2006
(119) TAT153, announced by Geron in 2010. 2010
(120) UP1 Works in UB1=UAGGGU-expressing cells, may be applied exogenously. 1998
(121) Product B from IsAGenix. December 2011.
for (122) - (153) dates, see the online list.

Some of these substances are poisonous, so check the links and descriptions for details. Results are somewhat tenative,
and more measurements are definitely in order, as well as comparative studies.
See [url="http://greenray4ever...TANUMERICSELECT"]http://greenray4ever...TANUMERICSELECT[/url] for full details.
I use telomerase activators for 2 weeks before using anti-cancer telomerase inhibitors for the next 2 weeks.

Edited by jamesagreen, 13 March 2013 - 07:51 PM.

[Freebytes]

Jamesagreen, Would you happen to know which of these are the best bang for the buck and which ones are easily and readily available from online sources such as Amazon? I would like something affordable that has the potential to lengthen telomeres.

[jamesagreen]

Today I would still say that the best verified telomerase activator is
astragalus root, taken at about 33 grams/day, or astragalus root extract taken at about 11 grams/day.
Astragalus root extract, rubbed into the scalp for a year, can often change one's hair color back to normal.
I use colostrum both internally and externally in solution on the skin, although I am uncertain about
how well it gets through the skin. Colostrum contains many telomerase-activating factors. Quite a bit
of astragalus root extract can be taken. Astragalus root extract in glycerin is delicious, and harks back
to the days when the rejuvenating effects of astragalus root were discovered in China, when
natives were brewing quite a bit of the extract and serving it up as Root Beer. Experimental dogs
can tolerate very large quantities of astragalus extract well.

Exercise boosts 14 endogenous telomerase activators and improves levels of cyclic AMP, which can
help restore senescent cells to normal form in the presence of EGF and/or PDGF from exercise or
colostrum. It helps to take creatine monohydrate (I recommend 6 STAR creatine X3 in tablets) to
synthesize cyclic AMP from ATP while exercising. Exercise also boosts levels of HIF-1 transcription
factor, especially when taking ginkgo biloba. HIF-1 works to improve hTERT transcription in every cell of the body.
Also, secretagogue supplements can be taken to boost the 14 different telomerase activators from exercise.

Another exciting telomerase activator that is easy to get and promising is milk thistle extract (silymarin),
taken at 10 grams/day, according to the Product B people. Nitric oxide at 5-10 grams/day is
a good telomerase activator for the endothelial lining, and Hawthorn, which improves heart muscle
tone, is a good telomerase activator, according to the Product B folks at IsAGenix, who have put many telomerase
activators into Product B.

Otherwise, it turns out that tissues derived from mesenchymal cells, such as connective tissues, cartilage, and bone,
express hTERT and hTR poorly unless a histone deacetylase inhibitor like Tricostatin A, sodium butyrate, or L-carnitine
is used to expand the cellular chromatin for transcription. Perhaps sodium butyrate, which is available as a supplement,
is the best choice: it is used in chicken feed to produce giant-sized chickens. L-carnitine, which is made
from methionine (from fish) and lysine in the presence of vitamin C, has just recently been identified as a
histone deacetylase inhibitor (HDAC inhibitor). When the chromatin is expanded, conventional telomerase
activators work better to produce hTERT and hTR transcripts to rejuvenate the cell by building longer telomeres,
which produces a more youthful pattern of gene transcription in the cell.

Quite a few experiments need to be done yet to determine how best to use available telomerase activators
and determine the relative cost advantages of them. Many expensive measurements of the effect of various telomerase
activators in situ on volunteers should be done, especially the $400 to $600 per measurement point sort done by
telomere measurement firms such as Repeat Diagnostics.

For my recent program, see http://greenray4ever...013march31.html and visit
http://greenray4ever.../longevity.html .

Edited by jamesagreen, 18 April 2013 - 07:03 PM.

[niner]

Another exciting telomerase activator that is easy to get and promising is milk thistle extract (silymarin),
taken at 10 grams/day, according to the Product B people. Nitric oxide at 5-10 grams/day is
a good telomerase activator for the endothelial lining, and Hawthorn, which improves heart muscle
tone, is a good telomerase activator, according to the Product B folks at IsAGenix, who have put many telomerase
activators into Product B.

I'm pretty sure that 5-10 g nitric oxide would kill you. Maybe you mean arginine? I don't think there is any evidence that silymarin or hawthorn can increase telomere lengths in humans. The Product B "Development Work", and I use that term charitably, consisted of some high concentration in vitro screening results. I do not expect it to work as a telomere extender in humans.

Edited by Lister, 26 April 2013 - 12:12 AM.

[Methos000]

If I interpreted Anthony's posts correctly, he seemed to be indicating that Product B induced telomerase weakly in vitro (to a lesser extent than TA-65, but greater than control). I wouldn't expect much induction in vivo either, given that result. Are there any Product B users out there with before/after telomere length test results to dispute this?

[Freebytes]

From I remember, it sounded like Anthony was saying that Product B did not work at all. Otherwise, I thought he might be selling it on his web site.

[Methos000]

Yes, at first he made it seem like his test had shown Product B to be essentially worthless. Later, he backed up a bit and and admitted that it had worked better than the control substance, and indicated that he might be interested in selling it. Apparently this would violate Isagenix rules, however.

[niner]

Yes, at first he made it seem like his test had shown Product B to be essentially worthless. Later, he backed up a bit and and admitted that it had worked better than the control substance, and indicated that he might be interested in selling it. Apparently this would violate Isagenix rules, however.

Did they test it on cells in a Petri dish? Kinda sounds like it, and it wouldn't surprise me if it worked at least a little bit in that kind of assay, because the compounds in Product B were discovered in an in vitro assay. Normally, the next step would be to see if it worked in animals, and if it did, then move to humans. Those two intervening steps were skipped by Isagenix, and it went straight to market. I think you'll have a hard time finding someone who can show you a before and after telomere test where telomeres were lengthened using Product B, because I don't think any exist. I don't think you can get the concentration of silymarin in the body that was used in the assay.

[abelard lindsay]

I noticed that B-Catenin presence increases hTert

http://www.ncbi.nlm....pubmed/22854964

Furthermore, we found that silencing endogenous β-catenin expression by β-cateningene-specific shRNA effectively decreased hTERT expression, suppressed TA, and accelerated telomere shortening.

and promotes regeneration without causing cancer

http://www.ncbi.nlm....pubmed/23125530

β-catenin, although fostering osteogenic differentiation, does not induce the malignant features and tumorigenicity conveyed by oncogenic H-RAS when introduced into partly transformed mesenchymal stem cells.

and is phosphorylated by cAMP/PKA.

http://www.ncbi.nlm....pubmed/18353896

In conclusion, we show a novel mode of regulation of endogenous beta-catenin through its phosphorylation by PKA, and we demonstrate the importance of this mechanism for ATP-induced proliferation of VSMC.

which is regulated to some extent by PDE4....

http://www.ncbi.nlm....pubmed/18276108

In addition, PKA specific activator N(6)-benzoyladenosine 3',5-cyclic monophosphate (N(6)Bz-cAMP) mimicked the effects of roflumilast. CREB phosphorylation by roflumilast was also inhibited by H-89, indicating that roflumilast protects SNP-induced apoptosis via PKA-dependent pathway.
...
In summary, our data indicate that roflumilast protects NO-induced apoptosis via both cAMP-PKA/CREB and Epac/Akt-dependent pathway

Is this the source of the PDE4 inhibition/Reservatol longevity connection?

http://www.ncbi.nlm....pubmed/22304913

As a consequence, resveratrol increases NAD(+) and the activity of Sirt1. Inhibiting PDE4 with rolipram reproduces all of the metabolic benefits of resveratrol, including prevention of diet-induced obesity and an increase in mitochondrial function, physical stamina, and glucose tolerance in mice. Therefore, administration of PDE4 inhibitors may also protect against and ameliorate the symptoms of metabolic diseases associated with aging.

So this makes me wonder... is CILTEP the new longevity stack ?

[hav]

I noticed that B-Catenin presence increases hTert

http://www.ncbi.nlm....pubmed/22854964

Furthermore, we found that silencing endogenous β-catenin expression by β-cateningene-specific shRNA effectively decreased hTERT expression, suppressed TA, and accelerated telomere shortening.

...

Found the full text here. They used Lithium Chloride, which is toxic, and cells grown in Wnt3a conditioned medium... would wnt3a cells be safe as a probiotic?? But I was recently reading up on osteogenesis techniques and saw a study that concluded using pulsed electromagnetic fields (a therapy recognized as safe) increased bone density via the β-catenin pathway:

Effects of pulsed electromagnetic fields on bone mass and Wnt/β-catenin signaling pathway in ovariectomized rats.

After 12-week interventions, serum 17β-estradiol and bone-specific alkaline phosphatase levels increased in the PEMFs group. Bone mineral density of the femur and the fifth lumbar vertebral body also increased in the PEMFs group. Histomorphometrical studies showed that PEMFs improved trabecular area, trabecular width, and trabecular number by 77.50%, 17.38% and 51.06%, respectively, and reduced trabecular separation by 44.28% compared with the OVX group. Biomechanical studies showed that PEMFs increased maximum load and energy to failure in the fifth lumbar vertebral body. Quantitative real-time RT-PCR analysis showed that PEMFs increased the mRNA expressions of Wnt3a, low-density lipoprotein receptor-related protein 5(LRP5), β-catenin, c-myc and runt-related gene 2 (Runx2), and reduced dickkopf1 (DKK1) in ovariectomized rats.

Howard

[Methos000]

Yes, at first he made it seem like his test had shown Product B to be essentially worthless. Later, he backed up a bit and and admitted that it had worked better than the control substance, and indicated that he might be interested in selling it. Apparently this would violate Isagenix rules, however.

Did they test it on cells in a Petri dish? Kinda sounds like it, and it wouldn't surprise me if it worked at least a little bit in that kind of assay, because the compounds in Product B were discovered in an in vitro assay. Normally, the next step would be to see if it worked in animals, and if it did, then move to humans. Those two intervening steps were skipped by Isagenix, and it went straight to market. I think you'll have a hard time finding someone who can show you a before and after telomere test where telomeres were lengthened using Product B, because I don't think any exist. I don't think you can get the concentration of silymarin in the body that was used in the assay.

Anthony had posted "...We use the same testing procedures used by UCLA to determine that TAT2 activated telomerase for Geron. You can also check that our CSO is a Visiting Scholar at UCLA that was trained by Geron."

I'm pretty sure that RevGenetics didn't invest the necessary resources for animal or human testing. It seems that no one else has either where Product B is concerned, unless the study that Product-B-User posted about is ever published.

[Mind]

Dr. Estep will be a guest on the LongeCity Now podcast, coming up soon. (founder of Telome)

[jamesagreen]

Today I would still say that the best verified telomerase activator is
astragalus root, taken at about 33 grams/day, or astragalus root extract taken at about 11 grams/day.
Astragalus root extract, rubbed into the scalp for a year, can often change one's hair color back to normal.
I use colostrum both internally and externally in solution on the skin, although I am uncertain about
how well it gets through the skin. Colostrum contains many telomerase-activating factors. Quite a bit
of astragalus root extract can be taken. Astragalus root extract in glycerin is delicious, and harks back
to the days when the rejuvenating effects of astragalus root were discovered in China, when
natives were brewing quite a bit of the extract and serving it up as Root Beer. Experimental dogs
can tolerate very large quantities of astragalus extract well.

Exercise boosts 14 endogenous telomerase activators and improves levels of cyclic AMP, which can
help restore senescent cells to normal form in the presence of EGF and/or PDGF from exercise or
colostrum. It helps to take creatine monohydrate (I recommend 6 STAR creatine X3 in tablets) to
synthesize cyclic AMP from ATP while exercising. Exercise also boosts levels of HIF-1 transcription
factor, especially when taking ginkgo biloba. HIF-1 works to improve hTERT transcription in every cell of the body.
Also, secretagogue supplements can be taken to boost the 14 different telomerase activators from exercise.

Another exciting telomerase activator that is easy to get and promising is milk thistle extract (silymarin),
taken at 10 grams/day, according to the Product B people. Nitric oxide from arginine at 5-10 grams/day is
a good telomerase activator for the endothelial lining, and Hawthorn, which improves heart muscle
tone, is a good telomerase activator, according to the Product B folks at IsAGenix, who have put many telomerase
activators into Product B.

Otherwise, it turns out that tissues derived from mesenchymal cells, such as connective tissues, cartilage, and bone,
express hTERT and hTR poorly unless a histone deacetylase inhibitor like Tricostatin A, sodium butyrate, or L-carnitine
is used to expand the cellular chromatin for transcription. Perhaps sodium butyrate, which is available as a supplement,
is the best choice: it is used in chicken feed to produce giant-sized chickens. L-carnitine, which is made
from methionine (from fish) and lysine in the presence of vitamin C, has just recently been identified as a
histone deacetylase inhibitor (HDAC inhibitor). When the chromatin is expanded, conventional telomerase
activators work better to produce hTERT and hTR transcripts to rejuvenate the cell by building longer telomeres,
which produces a more youthful pattern of gene transcription in the cell.

Quite a few experiments need to be done yet to determine how best to use available telomerase activators
and determine the relative cost advantages of them. Many expensive measurements of the effect of various telomerase
activators in situ on volunteers should be done, especially the $400 to $600 per measurement point sort done by
telomere measurement firms such as Repeat Diagnostics.

For my recent program, see http://greenray4ever...013march31.html and visit
http://greenray4ever.../longevity.html .

[pleb]

someone mentioned at the start of this thread SKQ1, SKQ1 is skulachevs ions i think someone on here has posted how to make it, although my memory may be wrong on the that,

Edited by pleb, 10 May 2013 - 10:55 PM.

[hav]

someone mentioned at the start of this thread SKQ1, SKQ1 is skulachevs ions i think someone on here has posted how to make it, although my memory may be wrong on the that,

http://www.opencures...rotocol_outline

Howard

[pleb]

Thanks Howard, i'll save that for the future,

[nowayout]

Telomerase inducers do not increase mouse lifespan: http://healthactivat...ephen-spindler/

[hav]

Telomerase inducers do not increase mouse lifespan: http://healthactivat...ephen-spindler/

Couldn't find a paper by Dr Spindler regarding telomerase and lifespan. I did find this:

Simvastatin increases lifespan

Here we show that simvastatin significantly increased the mean and maximum lifespan of Drosophila melanogaster (Drosophila)

Which kind of contradicts the idea that telomerase inducers don't increase lifespan since simvastatin is one of them.

Simvastatin upregulates Bcl-2 (75), a telomerase activator.

Howard

Edited by hav, 11 May 2013 - 05:20 PM.

[nowayout]

Telomerase inducers do not increase mouse lifespan: http://healthactivat...ephen-spindler/

Couldn't find a paper by Dr Spindler regarding telomerase and lifespan.

It's a pre-publication seminar.

[niner]

someone mentioned at the start of this thread SKQ1, SKQ1 is skulachevs ions i think someone on here has posted how to make it, although my memory may be wrong on the that,

Yeah, it's Skulachev's ion. I wouldn't dream of trying to make it when it's so easy to make c60-oo and it very likely works similarly (as a mitochondrial antioxidant) but probably a lot better.

Telomerase inducers do not increase mouse lifespan: http://healthactivat...ephen-spindler/

Here we show that simvastatin significantly increased the mean and maximum lifespan of Drosophila melanogaster (Drosophila)

Which kind of contradicts the idea that telomerase inducers don't increase lifespan since simvastatin is one of them.

Simvastatin upregulates Bcl-2 (75), a telomerase activator.

I don't think so. First of all, it's simvastatin in flies, not in a mammal. That's a huge difference. Secondly, we have no idea how much telomerase activation you would actually get from simvastatin in vivo, but I'll bet it isn't much.

[pleb]

( quote)
Yeah, it's Skulachev's ion. I wouldn't dream of trying to make it when it's so easy to make c60-oo and it very likely works similarly (as a mitochondrial antioxidant) but probably a lot better,.


to many ingredients there for me i'll also stick with C60-00 i don''t have enough pots and pans in the kitchen :>)

[hav]

someone mentioned at the start of this thread SKQ1, SKQ1 is skulachevs ions i think someone on here has posted how to make it, although my memory may be wrong on the that,

Yeah, it's Skulachev's ion. I wouldn't dream of trying to make it when it's so easy to make c60-oo and it very likely works similarly (as a mitochondrial antioxidant) but probably a lot better.

Telomerase inducers do not increase mouse lifespan: http://healthactivat...ephen-spindler/

Here we show that simvastatin significantly increased the mean and maximum lifespan of Drosophila melanogaster (Drosophila)

Which kind of contradicts the idea that telomerase inducers don't increase lifespan since simvastatin is one of them.

Simvastatin upregulates Bcl-2 (75), a telomerase activator.

I don't think so. First of all, it's simvastatin in flies, not in a mammal. That's a huge difference. Secondly, we have no idea how much telomerase activation you would actually get from simvastatin in vivo, but I'll bet it isn't much.

Actually, the simvastatin telomerase study was with human cancer cells which showed the activity via bcl-2 regulation. I only mentioned the simvastatin Drosophila longevity study because Dr. Spindler wrote it and his newly announced general principle that telomerase activation does not increase longevity is inconsistent with his own previous research. Maybe he'll eventually try to qualify his new conclusion as a broad distinction between mice and drosophila. But I'm suspicious of folks that start promoting studies they haven't published yet. It's usually because they're trying to sell something. Whatever it is, I'm not buying it just yet.

Howard

[niner]

But I'm suspicious of folks that start promoting studies they haven't published yet. It's usually because they're trying to sell something. Whatever it is, I'm not buying it just yet.

It's not unusual for scientists to talk about their work, or some aspect of their work, prior to publication. It kind of depends on the work. If you think you might get scooped and you're in hot competition with someone, you probably would keep quiet. But Spindler has done these big studies that no one else is doing, so he probably feels like it's safe to talk about it. He might have a paper in press, too. As far as something to sell, I'm not sure what Spindler would have to sell, other than ideas, like "CR is the one, the only, the true way..."

[nowayout]

But I'm suspicious of folks that start promoting studies they haven't published yet. It's usually because they're trying to sell something. Whatever it is, I'm not buying it just yet.

It's not unusual for scientists to talk about their work, or some aspect of their work, prior to publication. It kind of depends on the work. If you think you might get scooped and you're in hot competition with someone, you probably would keep quiet. But Spindler has done these big studies that no one else is doing, so he probably feels like it's safe to talk about it. He might have a paper in press, too. As far as something to sell, I'm not sure what Spindler would have to sell, other than ideas, like "CR is the one, the only, the true way..."

no, that is not his point of view. His explicitly expressed hypothesis is that he believes the same pathways behind CR longevity should be able to be activated by appropriate drugs, and the main reason we haven't found safe drugs that do that yet is that we haven't looked enough. That is why he is working on this large compound screening study.

someone mentioned at the start of this thread SKQ1, SKQ1 is skulachevs ions i think someone on here has posted how to make it, although my memory may be wrong on the that,

Yeah, it's Skulachev's ion. I wouldn't dream of trying to make it when it's so easy to make c60-oo and it very likely works similarly (as a mitochondrial antioxidant) but probably a lot better.

Telomerase inducers do not increase mouse lifespan: http://healthactivat...ephen-spindler/

Here we show that simvastatin significantly increased the mean and maximum lifespan of Drosophila melanogaster (Drosophila)

Which kind of contradicts the idea that telomerase inducers don't increase lifespan since simvastatin is one of them.

Simvastatin upregulates Bcl-2 (75), a telomerase activator.

I don't think so. First of all, it's simvastatin in flies, not in a mammal. That's a huge difference. Secondly, we have no idea how much telomerase activation you would actually get from simvastatin in vivo, but I'll bet it isn't much.

Actually, the simvastatin telomerase study was with human cancer cells which showed the activity via bcl-2 regulation. I only mentioned the simvastatin Drosophila longevity study because Dr. Spindler wrote it and his newly announced general principle that telomerase activation does not increase longevity is inconsistent with his own previous research. Maybe he'll eventually try to qualify his new conclusion as a broad distinction between mice and drosophila. But I'm suspicious of folks that start promoting studies they haven't published yet. It's usually because they're trying to sell something. Whatever it is, I'm not buying it just yet.

Howard

It's the other way around. It is very common for scientists (in academia) to discuss work in seminars or conferences pre-publication.

It is actually the ones who are trying to sell something (i.e., pharma company researchers) who don't do that.

[Logic]

"...In another aspect, the invention provides a composition comprising at least 20% human telomerase catalytic protein having a sequence encoded by a polynucleotide of the invention, the composition only exhibiting a telomerase catalytic activity if a telomerase RNA is added to the composition so as to associate with said protein. Such proteins capable of exhibiting telomerase catalytic activity may, for example, be characterized as having a defined motif that has an amino acid sequence:
Trp-R1-X7-R1-R1-R2-X-Phe-Phe-Tyr-X-Thr-GIu-X8-9-R3-R3-Arg-R4-X2-Trp
where X is any amino acid and a subscript refers to the number of consecutive residues, R1 is leucine or isoleucine, R2 is glutamine or arginine, R3 is phenylalanine or tyrosine, and R4 is lysine or histidine.

Equally, isolated, substantially pure or recombinant nucleic acid of the invention may encode a protein comprising an amino acid sequence:
Trp-R1-X7-R1-R1-R2-X-Phe-Phe-Tyr-X-Thr-Glu-X8-9-R3-R3-Arg-R4-X2-Trp.
The invention encompasses oligonucleotides and polynucleotides sharing substantial sequence identity or complementarity with a subsequence of such nucleic acids..."
http://www.freepaten.../EP0841396.html

How to easily get these peptides/proteins into each cell is another question! One that is not well answered in this patent?

[nowayout]

Patents are often speculative flights of fancy, filed just in case anything ever comes of it, so the fact that there is a patent should not be taken as indicative of anything realistic.

Edited by viveutvivas, 13 May 2013 - 05:10 PM.

[Mind]

The Dr. Estep interview is now available. He discusses telomerase inducers, about 2 or 3 minutes into the interview is when it gets more into telomeres/telomerse and testing.

[Logic]

Patents are often speculative flights of fancy, filed just in case anything ever comes of it, so the fact that there is a patent should not be taken as indicative of anything realistic.

I quite agree Viveutvivas, but this patent is held by Geron Corporation and the University of Colorado and Andrews, William H is one of the patent filers...
So I'm thinking they probably have the amino acid sequenced correctly.

Edited by Logic, 13 May 2013 - 09:50 PM.