LOG- C60+olive oil on 3 mice at home: a lifespan study
#31
Posted 06 June 2012 - 08:22 PM
#32
Posted 07 June 2012 - 06:08 PM
#33
Posted 08 June 2012 - 08:29 AM
#34
Posted 09 June 2012 - 10:07 AM
I use the small green balls to transport mice to/from the cage and the big ball to have them eat normal food:
balls-to-eat.png 278.53KB 17 downloads
The least I can say is that the treatment (including IF= 2 ad libitum meals per day) did not do harm to them. They are very curious, play with each other and climb everywhere:
mice-cage-play.png 418.81KB 20 downloads
#35
Posted 09 June 2012 - 10:12 AM
Thanks for your work AgeVivo. There's so cute
#36
Posted 09 June 2012 - 10:16 PM
Like in the paper, they will now be treated with C60oo only once a week. So the intermittent fasting has last only one week, and it will now be done only once a week for a few weeks, then once every other week for a few months: very little intermittent fasting overall.
#37
Posted 09 June 2012 - 11:24 PM
#38
Posted 10 June 2012 - 06:10 AM
Concerning testings, indeed I think that with a small grant I could test the content of what I am giving to the mice (do we detect C60 particles?) and could then also measure it in the pee of the mice. I am just not sure where to have c60 content tested inexchange of money (paid by longecity): anyone to investigate this, in a collaborative effort?
It could allow to choose to do smthg else than in the c60 paper: if with time c60 in pee becomes undetected and the animals appear to be long luved but start to obviously age (many many "if") then having the knowledge of such past c60 measures we might want to change the paper protocol and do another series of c60 treatments.
We could even have a rat owner do a similar test and one of those who tested c60 on themselves (...) do the pee test.
Just an idea, without having thought much about it.
Edited by AgeVivo, 10 June 2012 - 07:01 AM.
#39
Posted 12 June 2012 - 06:49 AM
Sumner SC et al. Distribution of carbon-14 labeled C60 ([14C]C60) in theZorba: " I have a question. Do the mice in these studies ever procreate? Just curious about testing C60 with respect to that -- something that might be important to humans considering taking it."
Here's a relevant study: http://www.ncbi.nlm....pubmed/20063269
They used radioactively tagged C60 and found that C60 does cross the placenta and does show up in milk, but they didn't mention any abnormalities as a result--at least not in the abstract.
pregnant and in the lactating dam and the effect of C60 exposure on the
biochemical profile of urine. J Appl Toxicol. 2010 May;30(4):354-60.
The c60-rat-life-extension corresponding author has read this thread and emailed me this: he reacted on this article that does not cite him, although his lab is actually pionnieer on the toxicity studies of c60 itself; he was the one to demonstrate in the early 90s that c60 could be dissolved in water and that it was possible to study its toxicity in a biological media, contrary to what others believed at that time; that massive doses (5g/kg) could be injected without any sign of toxicity (1996 paper, in vivo toxicology). And that it is only 10 years later that they started using aquous c60 solutions, without mentioning his work. That the ones who discoveted fulletenes received the Nobel prize for that in 1996 but that in the toxicology field in has been not so grateful to work on c60. This being said, here are in a following post some papers on c60 for those who want to study them carefully; the first 2 ones deal with the same topic as the 2010 paper mentionned here above.
Edited by AgeVivo, 12 June 2012 - 07:12 AM.
#40
Posted 12 June 2012 - 07:37 PM
#41
Posted 13 June 2012 - 01:36 AM
...
he was the one to demonstrate in the early 90s that c60 could be dissolved in water and that it was possible to study its toxicity in a biological media, contrary to what others believed at that time; that massive doses (5g/kg) could be injected without any sign of toxicity (1996 paper, in vivo toxicology).
...
Can you give a more complete ref for the 1996 paper, I would like to pull it.
thanks!
#42
Posted 13 June 2012 - 07:41 PM
the first 2 ones deal with the same topic as the 2010 paper mentionned here above.
Early effects of C60 administration in Swiss mice: a preliminary account for in vivo C60 toxicity (1996)
http://agevivo.com/l...bution-1996.pdf
C60 fullerene toxicity: preliminary account of an in vivo study
http://agevivo.com/l...-tox-of-C60.pdf
#43
Posted 13 June 2012 - 07:49 PM
#44
Posted 14 June 2012 - 03:53 AM
In case you were wanting to test on worms... http://www.wormbook....inmaintain.html
#45
Posted 14 June 2012 - 06:08 AM
What if you combine this treatment with other treatments shown to extend rodent life (such as Gene therapy extends mouse lifespan by 24 pc)?
What will be the telomerase length of the treated rodents compared to the control?
What if you gave the animals higher amounts of C60-Olive Oil and/or more frequent feedings?
What eventually kills the treated rodents, Amyloidosis?
In light of recent findings about the C1q protein (see: Secret of ageing found), how do the immune systems of the treated animals compare to control animals?
How do you recruit human volunteers and what is the benchmark for success in a human trial?
Nevertheless, this is exciting research!
#46
Posted 14 June 2012 - 06:27 PM
#47
Posted 17 June 2012 - 08:15 AM
I took some pictures this morning:
- some pictures taken before giving them C60oo, where each of them wants to be the one to go on the top of a ball and show that it can stand up on it
who will climb on?
both, but the white one pushed it away and stood up: bravo
souris-qui-dessus2.jpg 70.02KB 18 downloads souris-blanche-dessus2.jpg 47.94KB 20 downloads souris-blanche-debout2.jpg 68.19KB 19 downloads
then the black one climbs on and stands up: bravo too
souris-noire-dessus2.jpg 70.99KB 25 downloads souris-noire-debout2.jpg 49.81KB 19 downloads
- pictures of each mouse taking its piece of bread with C60oo (as always I rotated the mice so that there is one mouse at a time in the cage, taking C60oo)
c60oo1.jpg 55.5KB 16 downloads c60oo2.jpg 79.09KB 14 downloads c60oo3.jpg 48.56KB 19 downloads
- ok back to normal: food for all in the cage. the mice are now focusing on it
food4all.jpg 97.53KB 18 downloads
Edited by AgeVivo, 17 June 2012 - 08:18 AM.
#48
Posted 17 June 2012 - 10:29 AM
#49
Posted 17 June 2012 - 01:29 PM
Do you know the average lifespan of the type of mice you are using?
Also, I'm sure you don't need any more encouragement than the possibility of extending life but, have you considered entering the M-prize if you do succeed?
http://www.mprize.or...j_mprize_record
The longevity prize deals with single mice and the record is just under 5 years - (10% less than the 66 months of the rats in the study) Winning the M-prize could help fund a lot of further research - likely about $400k! There is also a category just for published research that Faathi et al should probably shoot for given the record is under 4 years in this category!
http://www.mprize.or...n=mj_mprize_how
http://en.wikipedia....tion#The_Mprize
#50
Posted 17 June 2012 - 05:04 PM
#51
Posted 24 June 2012 - 10:46 AM
The mice are doing well, they play with each other, sometimes walk upside down hung on the top of the cage: they do well. They took C60oo+bread without any hesitation: all is doing well, nothing special to report.
#52
Posted 01 July 2012 - 09:45 AM
Here are images of them taking the treatment:
BB-C60oo.JPG 25.1KB 15 downloads BW-C60oo.JPG 22.98KB 16 downloads W-C60oo.JPG 34.12KB 20 downloads
This week I have found the mice in even better shape than previous weeks, often playing together and hanging on the top of the cage. This morning, this was particularly explicit: sometimes the 3 of them were hanging and walking upside down during up to one minute; even when they were young adults I had not seen such long upside down walkings. This could be C60oo, it could be the week-end fasting, it could be the combination. Here are 2 pictures and a video of their upside down walkings:
3miceBWtop.JPG 39.74KB 20 downloads 3miceWtop.JPG 107.74KB 18 downloads (not able to upload video)
Edited by AgeVivo, 01 July 2012 - 09:46 AM.
#53
Posted 01 July 2012 - 12:44 PM
#54
Posted 05 July 2012 - 07:10 AM
it is very nice, that You have decided to take really some action and to do something yourself for
defeating the aging.
Sorry for the stupid question, but what exactly is C60oo ?
I googled for some hours, and I reached several substances, that are abriviatured as C60
including cobalt 60
Do You mean the C60 Fullerene molecule?
And what the to "oo" mean after the C60? Is "C60oo" equal to "C60"?
#55
Posted 05 July 2012 - 09:28 AM
AgeVivo,
it is very nice, that You have decided to take really some action and to do something yourself for
defeating the aging.
Sorry for the stupid question, but what exactly is C60oo ?
I googled for some hours, and I reached several substances, that are abriviatured as C60
including cobalt 60
Do You mean the C60 Fullerene molecule?
And what the to "oo" mean after the C60? Is "C60oo" equal to "C60"?
Read all about it here:
http://www.longecity...of-wistar-rats/
#56
Posted 05 July 2012 - 10:21 AM
So somebody else asks the same question like me - C60 is C60 Fullerene molecule and oo is olive oil
#57
Posted 08 July 2012 - 06:50 PM
Like in previous week-ends, the mice fasted overnight and took C60oo-on-bread this morning.
Here are images of them taking the treatment:
b1-080712.JPG 39.34KB 10 downloads b2-080712.JPG 48.36KB 12 downloads w-080712.JPG 36.12KB 8 downloads
I get the progressive impression that the mice are eating more than they used to. I have never been used to measure how much they eat (at home) so I am not sure; I am mentioning it in case someone could test in a lab. They do not look larger than before (*) so if they really eat more than normal (to be checked by someone?) it could be for example that C60oo reduces nutriment absorption : it could be some cryptoCR.
(*) contrary to humans in general mice naturally do not eat too much when fed ad libitum. In C60oo disturbs feeding behavior there is a risk in humans to become overweight for exemple. This is far extrapolation based on a tenuous impression, but I prefer to mention it, in case.
Edited by AgeVivo, 08 July 2012 - 06:56 PM.
#58
Posted 08 July 2012 - 07:03 PM
http://agevivo.com/l...-very-small.mp4
(at the very end of the video the third mouse also climbed upside down but I've stopped the video with my camera)
#59
Posted 15 July 2012 - 08:01 AM
Here are images of them taking the treatment:
b150712.JPG 29.63KB 10 downloads bw150712.JPG 35.93KB 13 downloads w150712.JPG 44.51KB 13 downloads
#60
Posted 18 July 2012 - 04:02 AM
Also tagged with one or more of these keywords: buckyballs, fullerenes, c60, mouse, mice, lifespan, olive oil, home, project, life extension
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