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c60 buckminsterfullerene antiaging c60 human trial c60 source vaughter wellness

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#61 Hebbeh

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Posted 01 July 2012 - 03:52 PM

Well, I still think the oil tastes very strong but my initial reaction, when I took the first sip was "I hope that C60 won't eati its way through my throat". I have never drunk pure olive oil so I never experienced that. Sadly I can't judge whether the strong taste is a rancid taste or not. My husband says the oil is fine, but then again, he's the major shareholder of our company :-)



Compare sampling some of the C60 processed oil with the unprocessed oil right out of the bottle. My oil in my C60 bottle is very mild tasting with no real flavor...unlike EVOO. I assume the C60 processing is having some effect on the oil? I can attest to feeling benefits from the C60 though. As reported earlier, my exercise stamina/endurance has definitely increased and I have less tolerance for caffeine. I did notice the slight "lung ache" that would briefly occur shortly after dosing but that seems to be lessening. Somebody described it as the burning that occurs in the lungs after being winded from a very vigorous aerobic workout and it did resemble that for me only milder. That was very concerning but it seems lessening with each day...either that or I'm getting used to it.

#62 SarahVaughter

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Posted 01 July 2012 - 05:52 PM

The processing: I watched the filtering of the oil. What happens is that a white frothy foam appears on top of the oil as it is filtered. This is not from air mixing with the oil, as some have suggested, but the opposite - all air is "boiling off" out of the oil, like Caisson disease, the air gets out of solution and becomes bubbles due to the reducion in pressure. I donät know whether that would make any difference. I was amazed that so much air was in the oil. Our overhead stirrers do not whip up the oil, we put them on a speed high enough to make the entire container swirl with a deep depression in the center, but not enough so that sounds appear, with air whipped into it.

I just took a sip of the half-liter bottle we will use in our next batch and it definitely has a slightly burning effect on the throat. It does not taste like stale peanuts as one would expect wit hstale oil, but it has a very rich taste, let's say there is a lot of taste to it. I don't know how to describe it better.

The price of the product is very high in comparison to the price of even the most expensive olive oil so we are open to all suggestions as to the very best oil, available by mail order, in bulk, to Europe.

It's now a few minutes after I tasted the oil (straight from the 0.5 liter bottle) and when I move my mouth I still feel a mildly "burning" taste. But I really can't explain the differences between oils in technical terms. We have several other, cheaper oils here and they smell and taste very differently. Milder, sweeter perhaps, much less "strong".

I have no more faith in the olive oil business and I've only read half of the first chapter of the book I mentioned..

However it is not known at this point how important the quality is. It would perhaps be much better to dissolve the oil in another oil, an oil that can dissolve much more C60 for example. But I guess we should stick as much to the original study as possible.

Edited by SarahVaughter, 01 July 2012 - 06:04 PM.


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#63 Edgar

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Posted 01 July 2012 - 06:12 PM

Sarah, if your oil is exposed to the air as it's stirred it will indeed oxidize it. This would not require a whipping or frothing of the oil; as the oil is stirred, oil below is continually brought to the surface and exposed to air. It would be useful to compare the taste of the oil both before and after stirring, as well as before and after filtering in order to assess the effects.

#64 SarahVaughter

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Posted 01 July 2012 - 06:35 PM

So you suggest to fill up Erlenmeyers all the way to the top and close them hermetically?

When we do that, the production capacity will be close to nil, in realistic terms.We have been thinking of buying a 50 l mixing vessel, we have to look into industrial-scale mixing under airtight conditions then..

I just put 1.5 ml of our C60-in-oilive-oil into my mouth. I don't recognize much, if any difference. I just had salad and soup before so my throat and palate was cleared of the previous oil. I definitely get the same slightly "burning" sensation that lingers. Perhaps there is a difference but I can't say it tastes significantly worse than the other oil.

Mind you, the oil from that bottle was the same brand but from 0.75 l bottles bought earlier. Could have been a different production run.

#65 SarahVaughter

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Posted 01 July 2012 - 06:39 PM

Another thing is the protective effect of C60 on the (per)oxidation of the oil. C60, being a powerful antioxidant, should prevent that to a certain extent, no?

#66 Hebbeh

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Posted 01 July 2012 - 06:51 PM

Another thing is the protective effect of C60 on the (per)oxidation of the oil. C60, being a powerful antioxidant, should prevent that to a certain extent, no?


We really don't know what C60 is doing. Not all antioxidants are the same or have the same characteristics...if they did, we would all just take a vitamin C tablet and call it a day. In regards to stirring the oil in the presence of oxygen or perhaps even dissolving a certain amount of oxygen in the oil during the stirring process (very likely), perhaps the oil should be stirred while sealed with an inert gas...or under vacuum.
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#67 Hebbeh

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Posted 01 July 2012 - 07:03 PM

I've begun a home brew experiment like others have done earlier, placing C60 in full bottles of high quality, highest polyphenol oil I could buy. I'm going low tech...shaking the bottles several times per day for 2 weeks....keeping them sealed in a dark, cool location. I don't plan on centrifuging or filtering the oil...as I'm not convinced that it is necessary. Remember, the original rat experiment was not a longevity experiment but a toxicology experiment....and I assume they took the extra step of centrifuging and filtering out any impurities so that the toxicity was on the C60 alone and not possibly affected by any contaminants or anything else in the oil. We are comparing apples to oranges in regards to what we hope to achieve and what the original experiment was intended to learn...and thus, the methods are not going to be entirely the same.

#68 zorba990

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Posted 01 July 2012 - 08:06 PM

This may be some helpful info:

Antioxidant protection of edible oils.

http://www.ncbi.nlm....pubmed/17285359
Results showed that sesame oil had the highest FRAP value (803 microM), followed by canola oil (400 microM), and sunflower, peanut, corn and olive oils (100-153 microM).
No idea if they used a high quality Olive Oil though...

#69 smithx

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Posted 01 July 2012 - 08:56 PM

So you suggest to fill up Erlenmeyers all the way to the top and close them hermetically?


How about filling the void space of the flasks with nitrogen, and then sealing them?

#70 zorba990

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Posted 01 July 2012 - 09:08 PM

So you suggest to fill up Erlenmeyers all the way to the top and close them hermetically?


How about filling the void space of the flasks with nitrogen, and then sealing them?


Looks like a pretty standard thing to do. So much so that there is a consumer oriented product for this:
http://boutique.oliv...t/can-inert-gas

#71 Hebbeh

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Posted 01 July 2012 - 09:38 PM

I'm also having a hard time believing it takes 2 weeks of constant stirring to dissolve the C60 into solution. I would think that under constant stirring, 24 hours would be a great plenty. I have to ask, are we really gaining anything by stirring beyond a day or two?

#72 SarahVaughter

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Posted 01 July 2012 - 09:50 PM

We still see some undissolved particles after an entire week of stirring..

#73 Hebbeh

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Posted 01 July 2012 - 10:19 PM

We still see some undissolved particles after an entire week of stirring..


Thanks Sarah. That answers my question...and explains why they stirred for 2 weeks in the original rat toxicology experiment. My low tech hand shaking will obviously take longer... :sad:

#74 Hebbeh

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Posted 01 July 2012 - 10:25 PM

I've begun a home brew experiment like others have done earlier, placing C60 in full bottles of high quality, highest polyphenol oil I could buy. I'm going low tech...shaking the bottles several times per day for 2 weeks....keeping them sealed in a dark, cool location. I don't plan on centrifuging or filtering the oil...as I'm not convinced that it is necessary. Remember, the original rat experiment was not a longevity experiment but a toxicology experiment....and I assume they took the extra step of centrifuging and filtering out any impurities so that the toxicity was on the C60 alone and not possibly affected by any contaminants or anything else in the oil. We are comparing apples to oranges in regards to what we hope to achieve and what the original experiment was intended to learn...and thus, the methods are not going to be entirely the same.


I had purchased 1 gram of C60 and 2 x 750ml bottles of oil for simplicity. I split the gram of C60 into 500mg and dumped into each 750ml bottle of oil....this should yield 0.667 mg/ml. With a saturation limit of .9 mg/ml, I'm hoping all of my C60 will have an easier time of getting into solution since it will be well below the saturation point.....hopefully.

Edited by Hebbeh, 01 July 2012 - 10:28 PM.


#75 Hebbeh

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Posted 01 July 2012 - 10:32 PM

And I apologize to Lister for taking his log slightly off topic....perhaps a Mod will want to consider merging the last couple pages into the main thread?

#76 Lister

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Posted 02 July 2012 - 04:21 AM

No Problem Hebbeh. I'm very interested in this conversation. I would suggest a separate topic regarding the best way to impart a pure red color into quality oil without Oxidizing. As was mentioned before it's a reasonable assessment to assume that oxidized oil won't affect the absorption of C60 into the body.

The reason we are taking this is for the benefits of C60 and not exclusively the Oil. Obtaining an acceptable sample of C60 Oil I would imagine will turn out to be more of an aesthetic issue.

I can't say that I have lots of experience with Oils but it would seem to me the best possible result would be clear inexpensive oil like Canola made to be nearly flavorless. This would adopt the red color more completely and I feel that inexpensive oil would allow for more capital for research and development of C60 as a product.

Sarah,

Thank you for joining our discussion here.

As I have said previously I’ve invested in 7 bottles total thus far. Through my doctor I’m going to be looking at finding researchers involved in Anti-Aging possibly at UBC. As I’m committed to this fairly risky experiment I don’t see why the scientific community can’t freely benefit. Hopefully someone is interested.

My roommate 10 years into Multiple Sclerosis and has been watching my results closely. I’m going to include her in this in about a week and I’ll post her results as well. As MS has been thought to be an issue with blood flow to the brain perhaps C60 may help.

Regardless I’m committed to Vaughter Wellness for better or worse. As I have done here I will continue to relay my source where I can.

On a new topic:

Nano Tubes have been noted for their electrical properties. They are extremely conductive. Considering C60 is part of the same Fullerene family could its electrical properties have an effect?

The reason I ask this is because we’ve been experiencing 2 results; cardio improvements and mental improvements. I’m not a neuroscientist but I have to wonder if C60s electrical properties can enhance electrical transitions throughout the brain. What are your thoughts?

#77 zorba990

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Posted 02 July 2012 - 04:39 AM

No Problem Hebbeh. I'm very interested in this conversation. I would suggest a separate topic regarding the best way to impart a pure red color into quality oil without Oxidizing. As was mentioned before it's a reasonable assessment to assume that oxidized oil won't affect the absorption of C60 into the body.

The reason we are taking this is for the benefits of C60 and not exclusively the Oil. Obtaining an acceptable sample of C60 Oil I would imagine will turn out to be more of an aesthetic issue.

I can't say that I have lots of experience with Oils but it would seem to me the best possible result would be clear inexpensive oil like Canola made to be nearly flavorless. This would adopt the red color more completely and I feel that inexpensive oil would allow for more capital for research and development of C60 as a product.

Sarah,

Thank you for joining our discussion here.

As I have said previously I’ve invested in 7 bottles total thus far. Through my doctor I’m going to be looking at finding researchers involved in Anti-Aging possibly at UBC. As I’m committed to this fairly risky experiment I don’t see why the scientific community can’t freely benefit. Hopefully someone is interested.

My roommate 10 years into Multiple Sclerosis and has been watching my results closely. I’m going to include her in this in about a week and I’ll post her results as well. As MS has been thought to be an issue with blood flow to the brain perhaps C60 may help.

Regardless I’m committed to Vaughter Wellness for better or worse. As I have done here I will continue to relay my source where I can.

On a new topic:

Nano Tubes have been noted for their electrical properties. They are extremely conductive. Considering C60 is part of the same Fullerene family could its electrical properties have an effect?

The reason I ask this is because we’ve been experiencing 2 results; cardio improvements and mental improvements. I’m not a neuroscientist but I have to wonder if C60s electrical properties can enhance electrical transitions throughout the brain. What are your thoughts?



Canola oil is junk. If you want a clear oil then use coconut oil (but the room would have to be warm enough). But none of that is replicating the experiment -- and there are still many unknowns. I'd be inclined to use sesame oil as a first substitution for olive oil, an unfiltered one like this: http://www.rawoils.com/sesame.htm

Over processed oils like canola contain toxic elements:
http://udoerasmus.co...s/udo/hbaco.htm
In addition, 0.5 to 1% of the oil molecules themselves are damaged during the processing. Some of these molecules have been isolated, tested with animals, and found to be very toxic. The damaged molecules found in cooking oils are much more toxic than the trans- fats which, according to the Harvard School of Public Health, double the risk of heart attacks, kill at least 30,000 people in the US every year, and increase diabetes.

C60 exhibits particle wave duality so perhaps a theoretical physicist would be good to add to the team:
http://en.wikipedia....iki/Matter_wave
Mitochondria emit biophotons: http://www.ncbi.nlm....pubmed/21425483
Light and tissue regeneration re biophotons: http://services.bepr...tissue_regen/2/

Edited by zorba990, 02 July 2012 - 04:40 AM.


#78 carbon

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Posted 02 July 2012 - 04:42 AM

At carbon60oliveoil.com we mix using 1 liter pyrex screw top glass beakers on magnetic stirrers for 2 weeks. We centrifuge using a Sorvall superspeed ss-3 centrifuge. We filter using a custom stainless steel pressure manifold running 15 psi through .22 micron syringe filters. Our oil turns out purple in small quantities and is a dark red in large quantity. We use certified organic extra virgin first cold press olive oil from the Mediterranean region and 99.9+ % purified C60 from SES Research.

#79 SarahVaughter

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Posted 02 July 2012 - 11:21 AM

Today we will centrifuge and filter our next batch, and I just smelled one of the mixing bottles and the smell is the same as the oil from a bottle straight from the shop - not bad, just an olive oil smell. I read that C60 prevents peroxidation of the oil. there is a Norwegian study that found that C60 was twice as effective as vit. E in preventing salmon go rancid.

We have just been on the phone with a supplier in Germany. He will design for us a mixing vessel for 50 liter (round flask) in which a stirrer can be hung with a vacuum seal. The flask has a tap at the bottom and several glass tube conections to which we can connect a vacuum pump and/or add Nitrogen.

So in the near future, all our batches will be mixed under those conditions.

We are also contacting Fathi Moussa (from the original rat study) and we'll try to get delivery of the same olive oil they used.
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#80 SarahVaughter

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Posted 02 July 2012 - 04:18 PM

The deep ruby red color of our product (photo made a few minutes ago):

http://c60antiaging....06/ruby-red.jpg

#81 zorba990

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Posted 02 July 2012 - 06:37 PM

The deep ruby red color of our product (photo made a few minutes ago):

http://c60antiaging....06/ruby-red.jpg


It looks brown in the dropper is this normal?

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#82 SarahVaughter

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Posted 02 July 2012 - 07:29 PM

Yes. Please refer to http://c60antiaging....0-in-olive-oil/

#83 zorba990

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Posted 02 July 2012 - 07:58 PM

At carbon60oliveoil.com we mix using 1 liter pyrex screw top glass beakers on magnetic stirrers for 2 weeks. We centrifuge using a Sorvall superspeed ss-3 centrifuge. We filter using a custom stainless steel pressure manifold running 15 psi through .22 micron syringe filters. Our oil turns out purple in small quantities and is a dark red in large quantity. We use certified organic extra virgin first cold press olive oil from the Mediterranean region and 99.9+ % purified C60 from SES Research.


Are you baking the SES fullerene to remove any extra solvents, or do you think the 99.9 SES research is pure enough?

#84 carbon

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Posted 03 July 2012 - 07:35 PM

Yes to both questions. I bake mine, but I don't think that it is necessary. Many of the people doing it at home seem to be getting great results. I also forgot to mention that my stirring is done in the dark of course.

Question for mods: Can we start another thread for people to review C60 olive oil producers? Right now it's just me and Mrs Vaughter producing this stuff, but several other sites are going up. People are going to be discussing the differences and I'm concerned about producer reviews taking over threads like this one.

#85 Lister

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Posted 03 July 2012 - 09:49 PM

It's been nearly 7 days now since my last dose of C60. I stopped taking it because I wanted to confirm that I could "escape" if I needed to. After 72 hours the majority of it had cleared. The possitive effects took 5 days to clear and now I'm back to normal. I really do notice the difference between when I was on it and now that I'm off it. I'm excited to get started again.

As far as neggative effects from stopping C60; there are none that I could tell. If you stop you stop and it clears your system. This is based off of a 14 day trial of 7 days on and 7 days off. I'll jump back on it again tomorrow, include my MS roommate and report the results in 4 days when it really starts to take effect.

#86 Hebbeh

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Posted 04 July 2012 - 12:21 AM

It's been nearly 7 days now since my last dose of C60. I stopped taking it because I wanted to confirm that I could "escape" if I needed to. After 72 hours the majority of it had cleared. The possitive effects took 5 days to clear and now I'm back to normal. I really do notice the difference between when I was on it and now that I'm off it. I'm excited to get started again.

As far as neggative effects from stopping C60; there are none that I could tell. If you stop you stop and it clears your system. This is based off of a 14 day trial of 7 days on and 7 days off. I'll jump back on it again tomorrow, include my MS roommate and report the results in 4 days when it really starts to take effect.


And how did you determine it cleared your system?

#87 Lister

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Posted 04 July 2012 - 07:35 AM

And how did you determine it cleared your system?


I guess I can't really determine if it's cleared my system or not. All I can say is nothing aches, I feel I have less energy, things seem exactly as they did before I started taking C60. Weren't there some predictions it could last in the system for years? Another study said it clears after 72 hours...

Feels like it's cleared my system at any rate.

#88 tintinet

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Posted 04 July 2012 - 11:42 PM

I think you're all loopy on placebo effects. Colored olive oil to me, so far.
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#89 Hebbeh

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Posted 05 July 2012 - 12:24 AM

I think you're all loopy on placebo effects. Colored olive oil to me, so far.



I'm all for whatever works! And I would think the rats would agree! ;) It's definitely doing "something" though. And plenty of research and patents are turning up for various biological uses...so placebo is highly unlikely...and the effects I've felt are solid.

#90 golden1

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Posted 05 July 2012 - 01:30 AM

Could you mix these with say piracetam or something? What does it take to meet the requirements for c60 to carry the molecule(this happens right?)? Depending on what you can have it carry besides olive oil components, maybe it would also facilitate the transport of other antioxidants/etc. Personally I'm thinking if the c60 do indeed carry molecules, which I thought was part of this and I know exists otherwise with molecules of other entrapment shapes than pure spheres(for example febreeze's active chemical), than it would be pretty interesting to maybe look into putting antioxidants or other low bioavailable things into the olive oil to increase their absorbtion and distribution. No idea how safe that is, but it seemed like an interesting avenue.





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