3 replies to this topic

[dws123]

DNA synthesis commonly refers to : DNA Relication ( DNA biosysnthesis esis), Polymerase chain reaction (enzymatic DNA synthesis), Oligonucleotide synthesis ( chemical sysnthesis of nucleic acids), Gene synthesis (physically creating artificial gene sequences).

[dws123]

DNA synthesizer is the process of synthesizing a gene in vitro without the need for initial template DNA samples. The main method is currently by oligonucleotide synthesis from digital genetic sequences and subsequent annealing of the resultant fragments. In contrast, natural DNA replication requires existing DNA templates for synthesizing new DNA.

[Danail Bulgaria]

How large sequences can be generated? Can a "whole" DNA of a human be generated?

[treonsverdery]

Human artificial chromosome


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A human artificial chromosome (HAC) is a microchromosome that can act as a new chromosome in a population of human cells. That is, instead of 46 chromosomes, the cell could have 47 with the 47th being very small, roughly 6-10 megabases in size, and able to carry new genes introduced by human researchers. Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first appeared in 1997. They are useful in expression studies as gene transfer vectors and are a tool for elucidating human chromosome function. Grown in HT1080 cells, they are mitotically and cytogenetically stable for up to six months.


Here is a link to placing artificial chrmosomes at human embryonic progenitor cytes http://hmg.oxfordjou...0/15/2905.short Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells

I wonder if anyone has generated a fully functional human tissue from a cyte with at least one chromosome (of a pair) where all the non gene "junk dna", as it has sometimes been called, although sometimes these are regulatory areas, has actually been removed? then they could place new genes of many megabases where the junk dna was. editing out the "junk" dna from just one of a pair of chromosomes would permit the pre edited chromosome to provide sufficient protein construction activity while permitting new proteins to be constructed at the reprogrammed junkless chromosome

Edited by treonsverdery, 22 August 2012 - 03:54 PM.