Hmmm not so sure about that. To me it feels like the positive effects fade away after max 1 week
What sort of effects are you seeing that fade after 1 week? How much c60-oo do you take?
Posted 20 February 2013 - 09:51 PM
Hmmm not so sure about that. To me it feels like the positive effects fade away after max 1 week
Posted 29 March 2013 - 09:05 PM
Hi bcelliott, thanks for posting! It's great to have someone with your expertise on board. What are your thoughts about the structure of the presumed C60 olive oil adduct? Olive oil is about 8% polyunsaturates, which might lead to a Diels-Alder coupling with C60, and an epoxide-mediated coupling is another thing that has been suggested as a way that C60 might react with saturated fatty acids. Franco Cataldo has discussed the spectrosopic evidence that C60 forms a compound with vegetable oils (refs are around here somewhere, I can find them if you'd like to take a look), but I don't think anyone has ever nailed down the structures. (I'm assuming more than one structure, given the mixed fatty acid composition of vegetable oils.)
I think several reactions are possible, depending upon oil temperature, level of oxygen exposure, and amount of light. Typical 1,2 addition products as well as Diels-Alder and multiple radical additions are certainly possible.
Out of curiosity, I looked for a charge transfer peak around 450 in the visible absorption spectrum of my homemade C60-OO, and found a very weak peak there. Mine was made by room temperature stirring in the dark with minimal oxygen, so I wouldn't expect a large yield of addition products under those conditions, but these products should increase with increased heat and oxygen. I would also expect that a clear bottle of C60-OO that has a violet color would gradually gain a reddish/brown tint over time if left exposed, since the majority of functionalized C60 compounds have a reddish color in solution.
Nailing down the identity of the addition compounds would be a bear of a problem--one I wouldn't enjoy! You'd have to chromatographically separate the products out of the oil into something like toluene, then collect enough to examine via NMR, mass spec, and electrochemistry. As you'll probably get a mixture of mono-, bis-, and higher poly-adducts of differing types having varying regiochemistry on the C60 cage, this would end up being an expensive, time-consuming project with little interest to most people. Sounds like the perfect grad student research project!
Posted 18 April 2013 - 05:09 PM
Posted 19 April 2013 - 06:47 PM
HHM,
I assume by now that you have tried the HyFn products you received. Any enoticeable effects worth mentioning?
Edited by HHM, 19 April 2013 - 06:51 PM.
Posted 22 April 2013 - 06:25 PM
HHM,
I assume by now that you have tried the HyFn products you received. Any enoticeable effects worth mentioning?
Yes I just finished the received HyFn. I haven't noticed any effect whatsoever. With c60-oo i did experience prononced effect on skin and hair
Posted 23 February 2015 - 07:30 PM
Last time I saw such an amazing list of properties the label said, "Finest Snake Oil". Is this likely to be real? C60 is certainly beginnning to look like the nearest thing to the elixir of life but that just makes me cautious, on the basis of the old adage, "if it looks too good to be true it probably is."
Last time I saw such an amazing list of properties the label said, "Finest Snake Oil". Is this likely to be real? C60 is certainly beginnning to look like the nearest thing to the elixir of life but that just makes me cautious, on the basis of the old adage, "if it looks too good to be true it probably is."
Last time I saw such an amazing list of properties the label said, "Finest Snake Oil". Is this likely to be real? C60 is certainly beginnning to look like the nearest thing to the elixir of life but that just makes me cautious, on the basis of the old adage, "if it looks too good to be true it probably is."
Posted 29 June 2015 - 07:14 PM
Posted 03 July 2015 - 03:46 PM
Edited by Walter Derzko, 03 July 2015 - 03:51 PM.
Posted 04 July 2015 - 06:25 PM
We used a spectrophotometric method and a method of biochemoluminescence to demonstrate that HyFnC60 in concentrations below 10(-9 )M down to 10(-23 )M stabilizes peroxidase, alkaline phosphatase, and bacterial luciferase against inactivation due to long-term incubation of the enzymes at room temperature and also against heat inactivation.
10**(-23) m/L * (6.02 e23 molecules/mole) = 6 molecules/liter.
I wasn't able to download the attachment for some reason, so I haven't seen the full text. If c60 is ordering water in a Pollack-like fashion, the ordered water must be pretty stable. This seems kind of implausible... It would take VERY little contamination to raise the c60 concentration by a factor of a million when dealing with concentrations of a handful of molecules per liter. This wouldn't necessarily negate the results they see at, say, femtomolar concentrations.
Posted 04 July 2015 - 08:01 PM
Posted 05 July 2015 - 02:36 PM
We used a spectrophotometric method and a method of biochemoluminescence to demonstrate that HyFnC60 in concentrations below 10(-9 )M down to 10(-23 )M stabilizes peroxidase, alkaline phosphatase, and bacterial luciferase against inactivation due to long-term incubation of the enzymes at room temperature and also against heat inactivation.
10**(-23) m/L * (6.02 e23 molecules/mole) = 6 molecules/liter.
I wasn't able to download the attachment for some reason, so I haven't seen the full text. If c60 is ordering water in a Pollack-like fashion, the ordered water must be pretty stable. This seems kind of implausible... It would take VERY little contamination to raise the c60 concentration by a factor of a million when dealing with concentrations of a handful of molecules per liter. This wouldn't necessarily negate the results they see at, say, femtomolar concentrations.
Only 6 Molecules C60 per Liter??
You could uptake a significantly higher level of C60 in Olive Oil.
What I find interesting is they demonstrate similar antibacterial effects found in the other C60 studies.
Study cited:
Fullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
Another Study Cited:
Stem cell niches provide resident stem cells with signals that specify their identity. Niche signals act over a short range such that only stem cells but not their differentiating progeny receive the selfrenewing signals1. However, the cellular mechanisms that limit niche signalling to stem cells remain poorly understood. Here we show that the Drosophila male germline stem cells form previously unrecognized structures, microtubule-based nanotubes, which extend into the hub, a major niche component. Microtubule-based nanotubes are observed specifically within germline stem cell populations, and require intraflagellar transport proteins for their formation. The bone morphogenetic protein (BMP) receptor Tkv localizes to microtubule-based nanotubes. Perturbation of microtubule- based nanotubes compromises activation of Dpp signalling within germline stem cells, leading to germline stem cell loss. Moreover, Dpp ligand and Tkv receptor interaction is necessary and sufficient for microtubule-based nanotube formation. We propose that microtubule-based nanotubes provide a novel mechanism for selective receptor-ligand interaction, contributing to the short-range nature of niche-stem-cell signalling.
Posted 06 July 2015 - 07:14 PM
Only 6 Molecules C60 per Liter??
You could uptake a significantly higher level of C60 in Olive Oil.
What I find interesting is they demonstrate similar antibacterial effects found in the other C60 studies.
Study cited:
Fullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
Another Study Cited:
Stem cell niches provide resident stem cells with signals that specify their identity. Niche signals act over a short range such that only stem cells but not their differentiating progeny receive the selfrenewing signals1. However, the cellular mechanisms that limit niche signalling to stem cells remain poorly understood. Here we show that the Drosophila male germline stem cells form previously unrecognized structures, microtubule-based nanotubes, which extend into the hub, a major niche component. Microtubule-based nanotubes are observed specifically within germline stem cell populations, and require intraflagellar transport proteins for their formation. The bone morphogenetic protein (BMP) receptor Tkv localizes to microtubule-based nanotubes. Perturbation of microtubule- based nanotubes compromises activation of Dpp signalling within germline stem cells, leading to germline stem cell loss. Moreover, Dpp ligand and Tkv receptor interaction is necessary and sufficient for microtubule-based nanotube formation. We propose that microtubule-based nanotubes provide a novel mechanism for selective receptor-ligand interaction, contributing to the short-range nature of niche-stem-cell signalling.
I found the part highlighted in red interesting. Does anyone know what studies they are referring to there?
Posted 06 July 2015 - 09:28 PM
Edited by Walter Derzko, 06 July 2015 - 09:32 PM.
Posted 06 July 2015 - 09:38 PM
Posted 07 July 2015 - 12:06 AM
Only 6 Molecules C60 per Liter??
You could uptake a significantly higher level of C60 in Olive Oil.
What I find interesting is they demonstrate similar antibacterial effects found in the other C60 studies.
Study cited:
Fullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
Another Study Cited:
Stem cell niches provide resident stem cells with signals that specify their identity. Niche signals act over a short range such that only stem cells but not their differentiating progeny receive the selfrenewing signals1. However, the cellular mechanisms that limit niche signalling to stem cells remain poorly understood. Here we show that the Drosophila male germline stem cells form previously unrecognized structures, microtubule-based nanotubes, which extend into the hub, a major niche component. Microtubule-based nanotubes are observed specifically within germline stem cell populations, and require intraflagellar transport proteins for their formation. The bone morphogenetic protein (BMP) receptor Tkv localizes to microtubule-based nanotubes. Perturbation of microtubule- based nanotubes compromises activation of Dpp signalling within germline stem cells, leading to germline stem cell loss. Moreover, Dpp ligand and Tkv receptor interaction is necessary and sufficient for microtubule-based nanotube formation. We propose that microtubule-based nanotubes provide a novel mechanism for selective receptor-ligand interaction, contributing to the short-range nature of niche-stem-cell signalling.
I found the part highlighted in red interesting. Does anyone know what studies they are referring to there?
http://www.ncbi.nlm....ubmed/21872392#
Fullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
PMID: 21872392
Edited by bixbyte, 07 July 2015 - 12:09 AM.
Posted 07 July 2015 - 04:00 AM
Only 6 Molecules C60 per Liter??
You could uptake a significantly higher level of C60 in Olive Oil.
What I find interesting is they demonstrate similar antibacterial effects found in the other C60 studies.
Study cited:
Fullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
Another Study Cited:
Stem cell niches provide resident stem cells with signals that specify their identity. Niche signals act over a short range such that only stem cells but not their differentiating progeny receive the selfrenewing signals1. However, the cellular mechanisms that limit niche signalling to stem cells remain poorly understood. Here we show that the Drosophila male germline stem cells form previously unrecognized structures, microtubule-based nanotubes, which extend into the hub, a major niche component. Microtubule-based nanotubes are observed specifically within germline stem cell populations, and require intraflagellar transport proteins for their formation. The bone morphogenetic protein (BMP) receptor Tkv localizes to microtubule-based nanotubes. Perturbation of microtubule- based nanotubes compromises activation of Dpp signalling within germline stem cells, leading to germline stem cell loss. Moreover, Dpp ligand and Tkv receptor interaction is necessary and sufficient for microtubule-based nanotube formation. We propose that microtubule-based nanotubes provide a novel mechanism for selective receptor-ligand interaction, contributing to the short-range nature of niche-stem-cell signalling.
I found the part highlighted in red interesting. Does anyone know what studies they are referring to there?
http://www.ncbi.nlm....ubmed/21872392#
J Hazard Mater. 2011 Oct 30;194:324-30. doi: 10.1016/j.jhazmat.2011.07.101. Epub 2011 Aug 5.Intratracheal administration of fullerene nanoparticles activates splenic CD11b+ cells.AbstractFullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
PMID: 21872392[PubMed - indexed for MEDLINE]
fullerenes intratracheally
Posted 07 July 2015 - 02:03 PM
http://www.ncbi.nlm....ubmed/21872392#
J Hazard Mater. 2011 Oct 30;194:324-30. doi: 10.1016/j.jhazmat.2011.07.101. Epub 2011 Aug 5.Intratracheal administration of fullerene nanoparticles activates splenic CD11b+ cells.AbstractFullerene nanoparticles ("Fullerenes"), which are now widely used materials in daily life, have been demonstrated to induce elevated pulmonary inflammation in several animal models; however, the effects of fullerenes on the immune system are not fully understood. In the present study, mice received fullerenes intratracheally and were sacrificed at days 1, 6 and 42. Mice that received fullerenes exhibited increased proliferation of splenocytes and increased splenic production of IL-2 and TNF-α. Changes in the spleen in response to fullerene treatment occurred at different time-points than in the lung tissue. Furthermore, fullerenes induced CDK2 expression and activated NF-κB and NFAT in splenocytes at 6 days post-administration. Finally, CD11b(+) cells were demonstrated to function as responder cells to fullerene administration in the splenic inflammatory process. Taken together, in addition to the effects on pulmonary responses, fullerenes also modulate the immune system.
PMID: 21872392[PubMed - indexed for MEDLINE]
fullerenes intratracheally
Indeed. Inhaled fullerenes which is perfectly legitimate if you're doing toxicology studies for environmental exposure but almost certainly not applicable to C60oo or hydrated fullernes.
Given that they that these inhaled fullerense were inducing inflammation, the fact that they were seeing immune system modulation isn't terribly surprising is it? I mean, how would you have inflammation without immune system modulation?
Edited by Daniel Cooper, 07 July 2015 - 02:05 PM.
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