3585 replies to this topic

[YOLF]

Are Kidney stones and amyloid made of the same or similar junk? Is it part of the same process?

[solarfingers]

In keeping with the thread C60 Experiments @ Home, I've uploaded a video to Youtube where I show how I am making a variable speed magnetic stirrer from a household fan...

Edited by solarfingers, 26 May 2013 - 12:37 AM.

[niner]

Are Kidney stones and amyloid made of the same or similar junk? Is it part of the same process?

No, totally different. Amyloid is made of layers of particular protein sequences that stick together. The most common is amyloid beta involved in Alzheimer's, and the one the afflicts most if not all supercentenarians is transthyretin, responsible for systemic amyloidosis. There are undoubtedly others. Kidney stones are mineral accretions. There are a few different kinds, but they are essentially crystals

[YOLF]

In keeping with the thread C60 Experiments @ Home, I've uploaded a video to Youtube where I show how I am making a variable speed magnetic stirrer from a household fan...

http://www.youtube.com/watch?v=tbqj2BU6Rfw

Can you devise a way to electrify the magnets? What dimension of neodymium magnet are you using.

[solarfingers]

Can you devise a way to electrify the magnets? What dimension of neodymium magnet are you using.

What would be the benefit of electrifying the magnets? The ones I am looking at are round at about 3/4ths of an inch. I've seen these before and they'll draw blood if you get your skin pinched between a pair. I don't think we need to worry about them loosing their magnetic potential so I can see no reason to electrify them. If they ever did get weak they can be easily replaced. I'll post another video when I am about to start stirring the c60-oo. I'll take it apart and show how the magnets are attached.

Thanks!

[Logic]

Can you devise a way to electrify the magnets? What dimension of neodymium magnet are you using.

Why? Electrify the magnets?
Whatever came out of the Hard Disk Drive.

Edited by Logic, 26 May 2013 - 01:58 AM.

[solarfingers]

Logic, you could use a magnet from a HDD yet I would have to sacrifice one but that's no good for my data...

[solarfingers]

I'm looking at the following Nalgene filtering system...



Does anyone have any experience with this system for c60-oo? What filtering system are you using?

[Logic]

Logic, you could use a magnet from a HDD yet I would have to sacrifice one but that's no good for my data...

Oh I see; you're thinking of electromagnets.
I'm the kind of person who has fans and old drives lying around. (IT guy)
A hardware shop should have a broken drive they will give you to strip.

[d4shing]

Are Kidney stones and amyloid made of the same or similar junk? Is it part of the same process?

not even close; Kidney stones are just calcium salts; amyloid is a misfolded protein

[YOLF]

Can you devise a way to electrify the magnets? What dimension of neodymium magnet are you using.

What would be the benefit of electrifying the magnets? The ones I am looking at are round at about 3/4ths of an inch. I've seen these before and they'll draw blood if you get your skin pinched between a pair. I don't think we need to worry about them loosing their magnetic potential so I can see no reason to electrify them. If they ever did get weak they can be easily replaced. I'll post another video when I am about to start stirring the c60-oo. I'll take it apart and show how the magnets are attached.

Thanks!

Adding electric would strengthen the field. Though I'm guessing you shouldn't have a problem given the size of your magnets.

[YOLF]

Are Kidney stones and amyloid made of the same or similar junk? Is it part of the same process?

No, totally different. Amyloid is made of layers of particular protein sequences that stick together. The most common is amyloid beta involved in Alzheimer's, and the one the afflicts most if not all supercentenarians is transthyretin, responsible for systemic amyloidosis. There are undoubtedly others. Kidney stones are mineral accretions. There are a few different kinds, but they are essentially crystals

Any relationship in catabolism by-products?

[pleb]

magnetising iron in the first place or ceramic magnets involves passing them through a electrical field

putting an electrical field round a permanent magnet could de magnetise it,

[YOLF]

Are Kidney stones and amyloid made of the same or similar junk? Is it part of the same process?

not even close; Kidney stones are just calcium salts; amyloid is a misfolded protein

Guess not, so how does the protien get misfolded? How might the of C60 on Alzheimers in rats be explained? Does it have anything to do with protein?

[solarfingers]

I'm only planning on using stationary magnets. There is no need in complicating the design with an electromagnet. Here is my proposed process:

1) Mix 400mg of c60 to 500ml of olive oil
2) Place it on the magnetic stirrer in the dark for three days
3) Filter the results through a .22 micron Nalgene filtering system (Could take a day)
4) Drink the whole 500ml in one shot - Joke

I'm actually thinking of taking a large dose up front and tapering off over the course of a couple of months (Kind of the same pace as the Baai study), taking a two month break and then repeat. I have yet to sit down and work out the numbers.

Edited by solarfingers, 26 May 2013 - 03:27 PM.

[zen]

I'm only planning on using stationary magnets. There is no need in complicating the design with an electromagnet. Here is my proposed process:

1) Mix 400mg of c60 to 500ml of olive oil
2) Place it on the magnetic stirrer in the dark for three days
3) Filter the results through a .22 micron Nalgene filtering system (Could take a day)
4) Drink the whole 500ml in one shot - Joke

I'm actually thinking of taking a large dose up front and tapering off over the course of a couple of months (Kind of the same pace as the Baai study), taking a two month break and then repeat. I have yet to sit down and work out the numbers.

Why do you think three days of mixing is enough?

As for the equipment you may want to take a look on http://www.longecity...492&qpid=589554 .

Also, filtering shouldn't take that long, if you use a good vacuum pump like for example http://www.amazon.co...0?ie=UTF8&psc=1

[solarfingers]

I'm only planning on using stationary magnets. There is no need in complicating the design with an electromagnet. Here is my proposed process:

1) Mix 400mg of c60 to 500ml of olive oil
2) Place it on the magnetic stirrer in the dark for three days
3) Filter the results through a .22 micron Nalgene filtering system (Could take a day)
4) Drink the whole 500ml in one shot - Joke

I'm actually thinking of taking a large dose up front and tapering off over the course of a couple of months (Kind of the same pace as the Baai study), taking a two month break and then repeat. I have yet to sit down and work out the numbers.

Why do you think three days of mixing is enough?

As for the equipment you may want to take a look on http://www.longecity...492&qpid=589554 .

Also, filtering shouldn't take that long, if you use a good vacuum pump like for example http://www.amazon.co...0?ie=UTF8&psc=1

You are correct I meant three weeks not three days... It won't let me correct it however.

Thanks for the leads on the equipment!

I'm only planning on using stationary magnets. There is no need in complicating the design with an electromagnet. Here is my proposed process:

1) Mix 400mg of c60 to 500ml of olive oil
2) Place it on the magnetic stirrer in the dark for three weeks
3) Filter the results through a .22 micron Nalgene filtering system (Could take a day)
4) Drink the whole 500ml in one shot - Joke

I'm actually thinking of taking a large dose up front and tapering off over the course of a couple of months (Kind of the same pace as the Baai study), taking a two month break and then repeat. I have yet to sit down and work out the numbers.

As for the equipment you may want to take a look on http://www.longecity...492&qpid=589554 .

This link does not work...

[hav]

...
I know I picked up the 10 day elimination idea somewhere. I'm glad that's cleared up. I would hate to continue espousing wrong information.
...

It might be from this statement in the Baati abstract, "Pharmacokinetic studies show that dissolved C60 is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours." But I'm not sure if that statement in the abstract is supported by the study as broadly as it is phrased. Their tissue and organ detection method consisted of examination with microscopes after sacrifice, looking for brown splotches believed to be c60 crystals. Which for the pharmacokinetic study in the quoted statement, would have been on animals sacrificed 48 hours after administration.

I'm thinking their statement only really applies to free c60 which might be able to form into a visible crystal or clump. And maybe only in places where the lipid that the c60 was attached to was oxidized or metabolized, thereby freeing the attached c60 in a place where no other lipids were available for the c60 to reattach to. In any event, I'm not sure if c60 that was still attached or adducted to a fat molecule would show up in any way using the type of microscope examinations they used. Might need to do some sort of tagging to really track it.

The paper mentions that their observations on accumulation and clearance do not apply to c60 complexed with liposomes:

In the case of tail vein administration [25] it is difficult to compare the data because C60 was complexed with liposomes. The scarceness of C60 crystals inside lung and kidney cells (Fig. 2) confirms the difference in behaviour of C60-liposome complexes, which mainly accumulates in lungs after tail vein administration [25].

So I'm guessing the quoted statement isn't applicable to c60 adducts that might be embedded in fatty membranes either. And, unfortunately they never released any organ or tissue analysis on rats that went the distance on c60. So we don't even really know for sure which form of c60 produced the longevity effect, the few c60 crystals they did detect early on or the c60 adducts that may or may not have accumulated short- or long-term in fatty membranes. More and better focused study is needed.

Howard

Edited by hav, 26 May 2013 - 05:19 PM.

[hav]

I'm looking at the following Nalgene filtering system...

Does anyone have any experience with this system for c60-oo? What filtering system are you using?

I use the 1-liter Autofil which looks very similar. I did experience cracking in the bottle after reuse but it may be due to the large bottle size. Thinking of trying a different brand, perhaps in the 500 ml size next time around and would be curious to find out how you make with these.

The pump I use is the Mityvac recommended earlier. Inexpensive, works well, and comes with an abundance of fittings and tubing.

Howard

Edited by hav, 26 May 2013 - 05:46 PM.

[solarfingers]

I use the 1-liter Autofil which looks very similar. I did experience cracking in the bottle after reuse but it may be due to the large bottle size. Thinking of trying a different brand, perhaps in the 500 ml size next time around and would be curious to find out how you make with these.

The pump I use is the Mityvac recommended earlier. Inexpensive, works well, and comes with an abundance of fittings and tubing.

Howard

Thanks Howard... I haven't had any success looking for an affordable Autofil. After Zen's post I realized that I actually have a hand pump in the garage for the car. If I change tubing it should be fine and it does appear as if it will work with the Nalgene filtering system. These are for medical use and aren't really industrial grade like the Autofil. It's likely going to be a bit flimsy. I'm going for inexpensive. I'll let everyone know how it goes.

I'll have to look into the Mityvac... I think my homemade magnetic stirrer will hold up to the challenge. I hope.

Edited by solarfingers, 26 May 2013 - 05:44 PM.

[hav]

Also, I spin magnetically for 2 weeks. Suggest if you buy a stirrer, stay away from Hanna... they burn out fairly quickly stirring oils. Benchmark is a little more expensive but has held almost a year so far without a problem. I went through 1 Hanna base model after a month and another heavy duty Hanna after a mere week before I switched brands.

Howard

Edited by hav, 26 May 2013 - 05:49 PM.

[stephen_b]

The equipment stuff might be better in the Equipment: mixing, centrifuging and filtering thread.

[stephen_b]

So, great result, BUT also consistent with the training I have been doing in preparation (averaging 3000 calories/34 miles a week peaking at 46 miles) for the 15 weeks prior to the race.

Just an anecdote, but over the past week I seem to be having better luck at a lower and more frequent dose (going from 15 ml biweekly to about 0.5 ml daily) with running performance.

In particular, my average heart rate at a range of paces from easy to fast has been noticeably lower. I hope it continues.

[zen]

This link does not work...

I am sorry, I meant http://www.longecity...-and-filtering/

[solarfingers]

Zen, thank you. I have been trying to give you a thumbs-up but my stupid browser (or Longecity) is not allowing me to...

[solarfingers]

I'm considering this as my protocol of c60 over a one year period of time. The starting max dosage is what Turnbuckle recommended in a previous post. I am cycling down the dosage from 1 - 10 months and will take a two month break before repeating.

Mixture:

400 mg c60 to 500 ml of Olive Oil = .8 mg/ml

400 mg c60 to 500 ml of Olive Oil = .8 mg/ml

200 mg c60 to 250 ml of Olive Oil = .8 mg/ml

These three mixtures over the year will expend all of my available c60...

Dosages:

1st month

12.5 ml a day = ~ 4.10 ml of c60-oo 3 X daily = 10 mg of c60 daily

2nd - 3rd months

6.25 ml a day = ~ 2.10 ml of c60-oo 3 X daily = 5 mg of c60 daily

4th - 6th months

3 ml a day = ~ 1 ml of c60-oo 3 X daily = 2.4 mg of c60 daily

7th - 10th months

1 ml a day = 1 ml of c60-oo 1 X daily = .8 mg of c60 daily

Quantities:

1st month

12.5 ml X 30 = ~300mg/375ml

2nd - 3rd months

6.25 ml X 60 = ~300mg/375ml

4th - 6th months

3 ml X 90 = ~216mg/270ml

7th - 10th months

1 ml X 120 = ~96mg/120ml


300
300
216
+ 96
====
912 mg


375
375
270
+120
====
1140 ml

~ These numbers are approximate as some rounding did occur in my calculations...

Edited by solarfingers, 27 May 2013 - 01:51 AM.

[Andre69]

How big was the dot, and how long did it last? A subconjunctival hemorrhage supposedly takes 10-14 days to clear. Is there anything there now that you could take a picture of? The second case really sounds like an allergic reaction. That's not to be dismissed, because allergic reactions can be very serious; I'd just like to get an accurate diagnosis. Antibiotics can mess up your gut and cause weird pains in your abdomen. In fact I just went through that myself a while back. Sounds like you had Zithromax, if it was a 5 day course. This has a very long half life, so it was actually in your system for closer to two weeks.

The dot was about 1mm big, I can't be exact but it was big enough to get scared of. I have tried to take some pictures but most of it is almost gone and you can barely notice anything.
I was prescribed Amoxicillin don't know what the difference is. Its in the penicillin family thats all I know.

I doubt that the olive oil caused any allergic reaction or the "kidney" pain, am originally Mediterranean and olive oil is a part of my daily diet. We even used to make our own olive oil too when I was younger.

Its almost a week now after I took C60 and am feeling alot better. No kidney pain, no muscle twiching,and I got a clear head at last. I believe its either the fullerenes or something in the olive oil that should´t be there that cause it.

I believe I will try from another supplier C60 maybe that will give me different results. If the results are the same then that would mean that am allergic to C60 fullerenes in EVOO. I have another unopened bottle which I don't know if am willing to try.

What surprised me is that this looney person had the same symptoms as I did. Not as dramatic as he described them, but still within a certain frame of plausibility.

Am wondering the colour of my bottle is not purple but rather more brownish kind of purple. Is that the result of the green of the olive oil and the purple colour of the C60?

Some general thoughts follow:

Some theorise that C60 attaches to the DNA and might cause it to unwind which usually happens when there is inflammation and this would mean more DNA damage. This would however contradict with the Baati study. Mice though don't age the same way humans do. Their metabolism is different and their oxidative stress compared to ours is far greater. If such an anti-oxidant like C60 was introduced to the mice it would be clear why they lived longer. Another example is that mice have somatic cells with long telomeres where as humans have short ones...however humans still live longer.
All am basically trying to say is we can not directly link the two together. Ofcourse if the study is correct then the results are too good to be true! I mean come on 90% longer life span? If you find a Ferrari on the internet for 100$ will you buy it? If it sounds too good to be true then its most probably not. That is what I was told and it came out true every time I went with it. I don't think I can say the same about C60 but I believe it will turn out to be a two-edged sword.

P.S. Are there any commercial suppliers you recommend? I found two that come up on google and I got mine form Sarah because she seemed to be the most trust worthy one (even though there was a case of mold in a bottle).

[solarfingers]

Andre69,

I read that study concerning the unwinding of DNA and it was only a simulation. I posted the same concern and thank you Niner for pointing that out. There is no evidence of c60 causing DNA damage when administered in a lipid form. In fact that would contradict the Baai rat study since there is no way the c60-oo administered rats would have lived nearly as long as they did and without any sign of cancer. They likely would have died in short order. It certainly would have caused early cell death and multiple cancers should the speculated DNA damage have occurred. As far as I know Sarah is one of the only reputable suppliers. From every angle, c60 appears to be safe and non-toxic in my honest opinion.

[YOLF]

I seem to remember a study done with C60 and Alzheimers. Did they manage some form of reversal or was it just a delay?

[solarfingers]

This is not a subject I am acquainted with yet... Here's what Google presents us with:

http://www.wired.com...eimers-disease/

http://www.nanowerk....potid=23726.php

What confuses me about this is my understanding that c60 suspended in water has some unwanted toxicity... Why drink the stuff? Am I off base in my understanding?