Equipment: mixing, centrifuging and filtering
#151
Posted 26 January 2013 - 04:22 AM
The N2 should be cold when it comes out of the canister, and should settle to the bottom.
Another thought would be take a chunk of dry ice and put it in a tea bag steeper. Suspect the ball a few inches above the oil. After the dry ice sublimates, the blender should be filled with CO2. Slowly remove the ball, and then blend.
#152
Posted 26 January 2013 - 05:09 AM
OK, here is my idea from reading this. The unmixed stuff settles to the bottom mostly, so why not pour off most of it, and then just pour the last part with the junk in there into the blender and turn it on for a few minutes.
It might be oversaturated -- lots of C60 without enough oil.
#153
Posted 26 January 2013 - 05:50 AM
OK, here is my idea from reading this. The unmixed stuff settles to the bottom mostly, so why not pour off most of it, and then just pour the last part with the junk in there into the blender and turn it on for a few minutes.
It might be oversaturated -- lots of C60 without enough oil.
It probably would be, but the particles should be now more smashed and any not dissolved yet should dissolve easily into the main body of oil once poured back into it, after a few hours or a few days. I am seeing a blender as more of a smashing of the big particles, then as a stirring machine when you didn't crush the C60 before mixing it with the olive oil.
We may be worrying way too much about getting air into the mix for a short period, since the air will rapidly leave and since both the oil and the C60 should be highly protected from any reaction to the oxygen by each other. Just my take
#154
Posted 27 January 2013 - 08:00 PM
#155
Posted 28 January 2013 - 01:58 PM
http://www.longecity...post__p__549500
I do not shake every day. About 3 minutes a week.
The color is now the same as Sara vaughter (http://c60antiaging.com). But there are still a lot of visible aggregates.
I hesitate to use the filter and the filter residue for testing with eugenol and limonene.
Perhaps placing the bottle well wrapped in clothes wringer?
#156
Posted 30 January 2013 - 04:53 AM
#157
Posted 30 January 2013 - 12:49 PM
The autofil units work great. I bought a case of the .22 micron half-liter style, and each is good for a liter or so of filtrate. Very little work involved. By comparison, expect the syringe filters to be a lot of work.Has anybody tried syringe filters? They seem to be the least expensive type of filter, but I've never used one so I don't know if they would work. I also don't know where to get glass syringes, or whether momentary contact between C60/EVOO and plastic would be bad.
Edited by Turnbuckle, 30 January 2013 - 12:50 PM.
#158
Posted 30 January 2013 - 01:00 PM
Has anybody tried syringe filters? They seem to be the least expensive type of filter, but I've never used one so I don't know if they would work. I also don't know where to get glass syringes, or whether momentary contact between C60/EVOO and plastic would be bad.
Contact between C60-oo and plastic is probably ok; at least as ok as contact between plain olive oil and plastic, which probably varies depending on the plastic. A lot of oils are sold in plastic bottles. (probably PETE) so that's presumably been deemed 'safe' by someone or other. For what it's worth, a lot of people don't filter. I grind the c60 into a very fine powder, mix with olive oil (in a glass container, FWIW) and shake occasionally over the next week or so. That's it. No mag stirrer, no centrifuge, no filter.
#159
Posted 30 January 2013 - 06:26 PM
The autofil units work great. I bought a case of the .22 micron half-liter style, and each is good for a liter or so of filtrate. Very little work involved. By comparison, expect the syringe filters to be a lot of work.Has anybody tried syringe filters? They seem to be the least expensive type of filter, but I've never used one so I don't know if they would work. I also don't know where to get glass syringes, or whether momentary contact between C60/EVOO and plastic would be bad.
Same setup here. But I also picked up a case of Kimax 45 ml glass bottles which cured cracks I was getting when reusing the Autofil bottles. I still see occasional small cracking in the screw area of the filter half but it doesn't seem to be causing a problem. Don't know if the similar competing products like Whatman, Nalgene, or TGSI are any better. I figure the case of Autofils I have will last about another year before I try another brand.
Also tried fine crushing the c60. The color change when mixing went faster but I still used a magnetic mixer for 2 weeks but got little change in filter residue. Out of concern that I might actually be getting more undissolved c60 smaller than 22 microns that gets past the filter, I'm going to discontinue the crushing in my next batch.
Howard
#160
Posted 30 January 2013 - 07:26 PM
The 500 ml vessels can't take a full vacuum. If you don't use more than 300 mm Hg, they should be fine. And I wouldn't worry about small particles not dissolving. The smaller the particle, the faster it should dissolve--a matter of surface area per volume. I grind a little at a time and then add oil drop-wise as I grind, and thereafter see very little filter residue after three days of stirring.The autofil units work great. I bought a case of the .22 micron half-liter style, and each is good for a liter or so of filtrate. Very little work involved. By comparison, expect the syringe filters to be a lot of work.Has anybody tried syringe filters? They seem to be the least expensive type of filter, but I've never used one so I don't know if they would work. I also don't know where to get glass syringes, or whether momentary contact between C60/EVOO and plastic would be bad.
Same setup here. But I also picked up a case of Kimax 45 ml glass bottles which cured cracks I was getting when reusing the Autofil bottles. I still see occasional small cracking in the screw area of the filter half but it doesn't seem to be causing a problem. Don't know if the similar competing products like Whatman, Nalgene, or TGSI are any better. I figure the case of Autofils I have will last about another year before I try another brand.
Also tried fine crushing the c60. The color change when mixing went faster but I still used a magnetic mixer for 2 weeks but got little change in filter residue. Out of concern that I might actually be getting more undissolved c60 smaller than 22 microns that gets past the filter, I'm going to discontinue the crushing in my next batch.
Howard
Edited by Turnbuckle, 30 January 2013 - 07:27 PM.
#161
Posted 30 January 2013 - 07:49 PM
] The 500 ml vessels can't take a full vacuum. If you don't use more than 300 mm Hg, they should be fine. And I wouldn't worry about small particles not dissolving. The smaller the particle, the faster it should dissolve--a matter of surface area per volume. I grind a little at a time and then add oil drop-wise as I grind, and thereafter see very little filter residue after three days of stirring.
I have the 1-liter autofils and the docs they came with mentioned 15 inch max but I kept the pressure under 10 (below 300 mm) and still occasionally got some cracking. Guess its just a fact of life with the disposable design. But far superior to the erlenmeyer flask I tried first. I saw a neat erlenmeyer-like setup once that wasn't plastic. Glass, ceramic, and metal I believe, that involved the filter being clamped in place. But I haven't been able to locate one like that for myself. Probably costs a fortune.
Howard
#162
Posted 30 January 2013 - 09:18 PM
#163
Posted 30 January 2013 - 09:37 PM
#164
Posted 31 January 2013 - 03:23 AM
http://www.amazon.co...m vacuum filter
There are also 250 mL and 1000 mL versions.
And here is a vacuum pump:
http://www.amazon.co...nd vaccuum pump
Are those the kind of components Turnbuckle and others are talking about? Does anybody know some palce that sells similar things for same or lower price as Amazon, perhaps direct from manufacturer?
#165
Posted 31 January 2013 - 03:26 PM
This is what I use--http://www.scientifi...7118&fullpage=yI searched on amazon and found a "Sterlitech - Autofil Laboratory Filtration System 500 mL Volume, 0.22 um Filter":
http://www.amazon.co...m vacuum filter
There are also 250 mL and 1000 mL versions.
And here is a vacuum pump:
http://www.amazon.co...nd vaccuum pump
Are those the kind of components Turnbuckle and others are talking about? Does anybody know some palce that sells similar things for same or lower price as Amazon, perhaps direct from manufacturer?
I connect it to a hand vacuum pump like the one you linked to. The case of 12 should be good for 12 liters, enough for a lifetime assuming you don't live forever. (It doesn't say Autofil, but that's what came from Tisch Scientific.) I wouldn't buy the full liter size as the pressure rating is less. The stand shown on the Amazon page is quite expensive but not necessary.
Edited by Turnbuckle, 31 January 2013 - 03:40 PM.
#166
Posted 31 January 2013 - 05:04 PM
Howard
Edited by hav, 31 January 2013 - 05:10 PM.
#168
Posted 31 January 2013 - 09:01 PM
Then this is different from the pedestal offered by Tisch, which has a vacuum hookup built in. The cost is almost $600.If you get a case (12 filters+bottles) directly from Sterltech, the pedestal is on special for $20.
Howard
I think they're the same..
pedestal.jpg 173.54KB 9 downloads.
#169
Posted 31 January 2013 - 09:19 PM
That's a heck of a deal! Twenty dollars when the list price is $543.64. The markup on these things must be obscene.Then this is different from the pedestal offered by Tisch, which has a vacuum hookup built in. The cost is almost $600.If you get a case (12 filters+bottles) directly from Sterltech, the pedestal is on special for $20.
Howard
I think they're the same..
pedestal.jpg 173.54KB 9 downloads.
Edited by Turnbuckle, 31 January 2013 - 09:19 PM.
#170
Posted 31 January 2013 - 09:21 PM
Wow, what's it made of, osmium?That's a heck of a deal! Twenty dollars when the list price is $543.64. The markup on these things must be obscene.
#171
Posted 31 January 2013 - 10:12 PM
Edited by zorba990, 31 January 2013 - 10:14 PM.
#172
Posted 01 February 2013 - 05:38 AM
#173
Posted 01 February 2013 - 02:49 PM
#174
Posted 05 February 2013 - 07:49 PM
Grinding C60 by mortar and pestle, putting into oil, and shaking sounds so much easier by contrast.
not to mention cost effective
#175
Posted 05 February 2013 - 08:43 PM
Grinding C60 by mortar and pestle, putting into oil, and shaking sounds so much easier by contrast.
not to mention cost effective
"shaking sounds" ... With what?
#176
Posted 05 February 2013 - 09:09 PM
Grinding C60 by mortar and pestle, putting into oil, and shaking sounds so much easier by contrast.
"shaking sounds" ... With what?
the word "sounds" belongs with "so much easier", as in 'seems so much easier'
#177
Posted 05 February 2013 - 09:39 PM
#178
Posted 06 February 2013 - 01:57 PM
i pour half the oil into a cup first that is then used to wash the spoons in after crushing and then added to the rest in the bottle,
#179
Posted 06 February 2013 - 05:00 PM
#180
Posted 06 February 2013 - 05:42 PM
Also tagged with one or more of these keywords: mixer, centrifuge, filter
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