1023 replies to this topic

[sensei]

I can concur that there is a subtle sensorium based experience after downing a 50ml/45mg bottle of C60OO.

 

I would not call it a "rush" in any sense of the word.  Perhaps an extremely, very very minor flush reminiscent of that from niacin but much much less.

 

I have not consumed 50ml of plain OO to provide a control experience, although I expect it is just that, a response to 500 calories of oil hitting ones stomach.

 

At teaspoon and tablespoon doses I have observed no such effect whatsoever; zip, zilch, nada.

[Kalliste]

The C60 "rush" might be an autonomic effect that occurs with potent antioxidants (c60, NAC) in some people, but for some reason it only occurs in the first few uses of the compound.  I have no idea what the mechanism might be, but the effect has been reported both here and in the literature (in a ref that I don't have now).  At any rate, it doesn't appear to have any negative consequences.  Another possibility is a placebo effect.  That wouldn't be surprising in the case of a very novel and poorly understood compound that's been reported to have miraculous effects.

 

I felt that effect the first time I took it. My hearbeat was elevated before taking it and the entire effect was grounded on the fact that I was using an experimental substanace for the first time. I have never felt any effect on heartbeats from c60 (or MitoQ for that matter).

Edited by Cosmicalstorm, 17 December 2014 - 07:24 PM.

[StevesPetRat]

It occurred to me, after reading a Turnbuckle citation, that it may be time to revisit NADH, AKA "biological rocket fuel", in the c60oo context; note that the aforelinked threads have been necrotic since just about the time that c60oo entered the mainstream press. In particular, can we use mitochondrial antioxidants as an ROS mitigation mechanism, in order to safely unleash the supercharging potential of NADH on neuronal and muscular systems? (diana_2000 suggested cycling NADH; c60oo would require a reevaluation of that recommendation.) We might also need sodium ascorbate, liposomal vitamin C, alpha lipoic acid, or some other extramitochondrial antioxidant in order to subsume the full ROS blast radius. But if it works, we might end up with a superathlete with unusually low response latency and perhaps enhanced cognition. Any takers?

Well, I took 200 mg (10 x 20 mg) of sublingual NADH the other day (OK, to be honest, a lot of it was crammed into my lower lip after I ran out of sublingual real estate). I might have felt a little warmer than usual. No other effects noticed. Since many people "feel something" from as little as 5 mg, I suspect there are some cogs jammed up elsewhere in my metabolism... Or NADH sucks.

Edit: Or I blacked out and ran a marathon.

Edited by StevesPetRat, 30 December 2014 - 06:10 AM.

[Adamzski]

I had a rush the first time I took 1ml of carbon c60 again after not having any for a year

 

http://www.longecity...ndpost&p=689709

[sensei]

I had a rush the first time I took 1ml of carbon c60 again after not having any for a year

 

http://www.longecity...ndpost&p=689709

 

I really, really, really, think that is psychosomatic in nature.

 

1ml is 1/5 of a teaspoon

 

And FYI I did the 50ml of plain olive oil test -- yup, same sort of really, really mild flush -- i expect it is from that much oil (500 calories) hitting the stomach.

 

 

Note: I dose a full 50ml/45mg C60OO bottle at a time, sometimes more than 1 bottle.

[smccomas01]

I second that on the initial rush being a possible placebo. I have been downing between 40 and 50 ml in a shot (using a shot glass) no rush or flush to speak of.  

[niner]

You guys that think it's placebo, what do you think of the discussion in post 442?  The effect described there only happens the first time or two that you take particularly potent antioxidants, and then only in some people.  It's been reported here by a number of people; it takes various forms, but they fall under a broad heading of "weird autonomic effects".  I didn't notice it myself, but I've seen a lot of reports of it, and it's been mentioned in the literature.

[smccomas01]

To a degree yes, I have been thinking about this for a while and I have made some observations.

 

It would seem that we tend to focus on whether a person "feels" something reading through the nootropics threads it normally goes like this, took (insert name of something) and report of whether the person felt an effect or not.

 

When I first started dosing with C60 I believed that I felt a rush or boost of energy in retrospect I currently believe this to be mostly placebo. That explains the perceived loss of effect.

 

However in my opinion what is more of an indicator is what I do not feel. I do not feel tired during the work day, the number of aches and pains from the RA has been greatly reduced. I am physically stronger now, I lost a considerable amount of muscle mass when the RA was raging and after 6 + months of being on Enbrel it had not returned. After being on C60 I can see an increase in the size of my biceps this was gradual process and it was not expected.

 

Think about this does a person "feel" there hair going grey or turning black.  

[resveratrol_guy]

Quick note: I don't want to go back to the whole aquarium fish discussion which evidently wasn't popular here, but I do feel it worth mentioning that I've noticed a sharp decrease in monthly fish death rate (about 60%) and a complete shutdown of infectious episodes since starting them (about 40 fish) on c60oo (basically a monthly megadose at perhaps 30% of caloric intake for one day). It's now standard operating procedure for me when introducing new fish in any of my tanks. Not that this is surprising, but obviously fish are easier to study than humans, and might expedite the optimization of c60oo delivery and dosing. And to anyone who finds this post and wants to run an experiment, I suggest petstore variety xiphophorus variatus. They are heavily inbred in order to accentuate their colors, as evinced by their high rate of spinal and fin deformity. As a result, their immune systems are remarkably inferior to those of wild fish of the same genus, both of which I keep. Previously, I had a severe loss rate within a couple weeks of introduction of the petstore ones; this is the most notable improvement due to c60oo dosing.

 

Edited by resveratrol_guy, 01 January 2015 - 03:37 AM.

[mait]

Quick note: I don't want to go back to the whole aquarium fish discussion which evidently wasn't popular here, but I do feel it worth mentioning that I've noticed a sharp decrease in monthly fish death rate (about 60%) and a complete shutdown of infectious episodes since starting them (about 40 fish) on c60oo (basically a monthly megadose at perhaps 30% of caloric intake for one day). It's now standard operating procedure for me when introducing new fish in any of my tanks. Not that this is surprising, but obviously fish are easier to study than humans, and might expedite the optimization of c60oo delivery and dosing. And to anyone who finds this post and wants to run an experiment, I suggest petstore variety xiphophorus variatus. They are heavily inbred in order to accentuate their colors, as evinced by their high rate of spinal and fin deformity. As a result, their immune systems are remarkably inferior to those of wild fish of the same genus, both of which I keep. Previously, I had a severe loss rate within a couple weeks of introduction of the petstore ones; this is the most notable improvement due to c60oo dosing.

 

Thanks for effort. Another interesting data point about C60 indeed. I might be overly greedy but I wonder if it is possible to extend this experiment a little by using two tanks of fish of same variety from same genus. One tank is dosed with C60, other is not.  This would give a rough estimation of effect of C60 on fish lifespan. Because fish You are using seem to be relatively small it would enable a larger number of test organism to be used. This would give pretty good estimation of distribution of lifespan and mortality curves between C60 and control groups of fish. 100 fish on both tanks would give pretty good statistical power to this experiment.

 

The idea of using fishes as test organisms is not as far-fetched as it would seem at first. For example zebrafish is increasingly been used in experimental biology. Even animal behaviour and neuropsychopharmacology related experiments are carried out by using zebrafish as test organism.  After some Google scholaring there seems to number of articles that have used zebrafish to study ageing.

Edited by mait, 01 January 2015 - 03:27 PM.

[Xerxes]

Hmmm I'm rather interested in doing an experiment like this.. 2 identical tanks with zebrafish.. My only question (two actually); how do you feed fish C60 in oil? Won't the oil just sit on top - or do the fish go to the top and eat the oil as they would with fish flakes.. Also, is a filter necessary if a half-third of the water is replaced daily/every other day?

 

 

[mait]

To my surprise zebrafish seems to have longer lifespan than rodents. From purely practical standpoint of caring out lifespan experiment it may be disadvantageous. Still it may have its merits – short lived model organisms may have different main mechanisms that lead to age related death than longer lived organism. If C60 treatment manages to increase the lifespan of already long lived species it may have some validity for humans too.

 

From http://www.sciencedi...568163707000098

 

The zebrafish has emerged over the past decade as a major model system for the study of development due to its invertebrate-like advantages coupled with its vertebrate biology. These features also make it a potentially valuable organism for gerontological research. The main advantages of zebrafish include its economical husbandry, small yet accessible size, high reproductive capacity, genetic tractability, and a large and growing biological database. Although zebrafish life span is longer than rodents, it shares the feasibility of large-scale mutational analysis with the extremely short-lived invertebrate models. This review compares zebrafish with the more widely used model organisms used for aging research, including yeast, worms, flies, mice, and humans.

 

 

In addition ageing in zebrafish has been related to ROS and insulin signalling, which is similar to mammals.

 

From http://www.sciencedi...531556503002900

 

Fish represent approximately half of all vertebrate species, yet have received little attention as models for aging research relative to invertebrate organisms or rodents. However, the basic gerontological characteristics of several fish species have been studied and provide compelling data for further investigation. In particular, guppies have proved to be an invaluable model for evolutionary analyses of aging, killifish are short-lived and may be exploitable for life span manipulation studies, and zebrafish come with a formidable armament of associated biological tools from their widespread use as a model of vertebrate development. These fish are well suited for the investigation of basic processes implicated in aging, such as insulin signaling, oxidative stress, and comparative studies of species with widely divergent longevities. Under-explored areas for which these fish may also provide unique research opportunities include their use as platforms for disease modeling, drug discovery, and regenerative medicine.

 

[resveratrol_guy]

Moved the fish/c60oo discussion here, which is probably the most appropriate thread.

[pone11]

I wanted to inform this thread that I had recently created a new thread to discuss if there are other species that might be better for C60 testing:

http://www.longecity...or-c60-studies/

 

Oops, just saw that another poster pointed to the same thread.  Unfortunately, this site does not let me delete posts?

Edited by pone11, 02 January 2015 - 11:57 PM.

[sensei]

I have just noticed that a deep scar caused by a cut to the bone on my finger has become much less evident. It is not lumpy and seems to be disappearing.  This leads me to believe that tissue remodeling (maybe stem cell genesis or upregulation) is caused by C60OO at the dosage I'm taking.

 

I can also report that my sense of smell -- that was very good to begin with, is off the charts.  I can also smell the difference between people now. 

[ceridwen]

It affects my sense of smell too. I didn't use to be able to smell anything at all. Now I can as I now take C60.
It affects my sense of smell too. I didn't use to be able to smell anything at all. Now I can as I now take C60.

[niner]

I've been taking c60 for a couple years now, and recently my sense of smell went to hell in a handbasket.  I think this was caused by a viral infection.  I'm just hoping it comes back.

[Turnbuckle]

I have just noticed that a deep scar caused by a cut to the bone on my finger has become much less evident. It is not lumpy and seems to be disappearing.  This leads me to believe that tissue remodeling (maybe stem cell genesis or upregulation) is caused by C60OO at the dosage I'm taking.

 

I can also report that my sense of smell -- that was very good to begin with, is off the charts.  I can also smell the difference between people now. 

 

 

I haven't noticed the improvement in smell, but I have noticed other effects--hair regrowth, nail health, and the disappearance of scars, that are very suggestive of a boost in stem cell activity. So I think we are seeing a bi-modal effect. C60 is a good mitochondrial antioxidant, maybe even better than glutathione, and that effect doesn't fade with time. You can take it and drink all night and not have much in the way of negative effects the next day, and you can do that for a long time. But there is apparently a second effect where it stimulates the activity of mitochondria and thereby stimulates the differentiation of stem cells, and this effect does fade over time. It fades for one of two reasons--or both--that stem cells are becoming depleted, or that the boost in mitochondrial activity is subject to homeostasis--as any change in the activity of mitochondria is likely to be.

[sensei]

 

I haven't noticed the improvement in smell, but I have noticed other effects--hair regrowth, nail health, and the disappearance of scars, that are very suggestive of a boost in stem cell activity. So I think we are seeing a bi-modal effect. C60 is a good mitochondrial antioxidant, maybe even better than glutathione, and that effect doesn't fade with time. You can take it and drink all night and not have much in the way of negative effects the next day, and you can do that for a long time. But there is apparently a second effect where it stimulates the activity of mitochondria and thereby stimulates the differentiation of stem cells, and this effect does fade over time. It fades for one of two reasons--or both--that stem cells are becoming depleted, or that the boost in mitochondrial activity is subject to homeostasis--as any change in the activity of mitochondria is likely to be.

 

 

C60 induces stem cell differentiation

 

Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

 

"Here, we investigated an extremely powerful antioxidant, polyhydroxylated fullerene (fullerol),17–19 and report for the first time that antioxidative fullerol enhanced osteogenesis and reduced reactive oxygen species (ROS) in human ADSCs, as well as increased expression of FoxO1, a major isoform of forkhead box O transcription factors that defend against ROS and promote osteoblast differentiation in bone.20,21"

 

http://www.ncbi.nlm....les/PMC4149442/

[Turnbuckle]

 

 

I haven't noticed the improvement in smell, but I have noticed other effects--hair regrowth, nail health, and the disappearance of scars, that are very suggestive of a boost in stem cell activity. So I think we are seeing a bi-modal effect. C60 is a good mitochondrial antioxidant, maybe even better than glutathione, and that effect doesn't fade with time. You can take it and drink all night and not have much in the way of negative effects the next day, and you can do that for a long time. But there is apparently a second effect where it stimulates the activity of mitochondria and thereby stimulates the differentiation of stem cells, and this effect does fade over time. It fades for one of two reasons--or both--that stem cells are becoming depleted, or that the boost in mitochondrial activity is subject to homeostasis--as any change in the activity of mitochondria is likely to be.

 

 

C60 induces stem cell differentiation

 

Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

 

"Here, we investigated an extremely powerful antioxidant, polyhydroxylated fullerene (fullerol),17–19 and report for the first time that antioxidative fullerol enhanced osteogenesis and reduced reactive oxygen species (ROS) in human ADSCs, as well as increased expression of FoxO1, a major isoform of forkhead box O transcription factors that defend against ROS and promote osteoblast differentiation in bone.20,21"

 

http://www.ncbi.nlm....les/PMC4149442/

 

 

Stem cells (like cancer cells) inhibit oxidative phosphorylation in favor of aerobic glycolysis for energy production. This is done by decoupling the stem cells' mitochondria. Reverse that and you can force them to differentiate into somatic cells. So how could C-60 up-regulate mitochondria? One possibility is that C60 is simply plugging the UCP pores that mitochondria use to decouple  oxidative phosphorylation from ATP synthesis. The size of a C-60 molecule with adducts (or fullerol) is about right to serve as a plug.

Edited by Turnbuckle, 05 January 2015 - 03:54 AM.

[aribadabar]

Guys,

 

I accidentally came across the Superball:

 

Glycosidase Inhibition with Fullerene Iminosugar Ball: a Dramatic Multivalent Effect
Angew. Chem. Int. Ed. 2010, 49, 5753.

Superball! Even if considerable interest has been recently directed towards the design of specific glycosidase inhibitors, only few studies have been performed using multivalent glycomimetics. Biological results reported to date with di- to tetravalent iminosugars were rather disappointing. We have recently designed an unprecedented multivalent iminosugar based on a C₆₀ scaffold. A unique globular platform presenting iminosugars with a defined valency of 12 was synthesized by click chemistry. The inhibitory activity of this iminosugar ball was found to be significantly higher than that of the corresponding monovalent ligand for most glycosidases, with a binding enhancement up to 2100 for Jack bean α-mannosidase (180-fold enhancement per iminosugar!).

 

Can niner and/or Turnbuckle decipher what this means besides looking super cool  

[aribadabar]

I wanted to report another effect I have noticed. I came across this hearing test about 3 weeks ago and initially I was able to hear until 14kHz (I will be 34 in 1.5 months). As mentioned earlier , I am taking 45 ml C60 every other day for the last 3 weeks too and kept taking the test a few times per week with no discernible improvement.

Today, I was able to hear slightly the 15kHz sound. The tests have been performed at my home PC with the same (cheap) earphones so I suspect C60 may have helped. Now I also have been taking 10mg MitoQ per day during the same period - so not sure which one would be the contributing factor. AFAIK, MitoQ doesn't have stem cell activation properties necessary for such effect so I ascribe the improved hearing to C60.

I will continue the experimentation to see if it was a fluke or a robust improvement.

Thoughts, comments are welcome. 

 

The bursts of heightened smelling ability reported earlier also persist.

 

 

I also look forward to hearing any comments about the Superball above. What does it really mean? Do you think it could be beneficial for us? Does this require specialized lab equipment or can it be done at home?

 

Thanks!

[cytg]

Short story

After a divorce i've been turning to a therapist best known to man as ms. alcohol, now given some time this is mostly under control but i do still have the occasional binge. 

I've done c60 before and I did notice this 'effect' before but cast it off as placebo or something else. 

Now having taking a break from c60 and just recently gotten back on it. Last night I had a small recession wich involved a whole bottle of jack and a bottle of wine, this would have knocked me out for a solid 24h, headaches and general uncomfort following. I am typing this 12h after the first drop and about 6 hours before i tumbled over in my bed. I should not be this 'awake' or to my senses as I currently am. I can feel that I am still under the influence but in a few hours I will give myself a home administred alcohol test to check it off.

Just wanted to share.

[Walter Derzko]

I still wonder what a little DMSO/c60/OO would do for wrinkles. I know it's used with arnica oil.

 

DMSO is toxic and a mutagen (See MSDS for DMSO)   

[mikey]

 

I still wonder what a little DMSO/c60/OO would do for wrinkles. I know it's used with arnica oil.

 

DMSO is toxic and a mutagen (See MSDS for DMSO)   

 

 

"DMSO is toxic and a mutagen"

 

I'm sorry, Walter. You should dig a bit deeper than to quote the MSDS piece, as a reference. Those write-ups must state the absolute worst-case potential, whether they are backed by solid science or not.

That statement is, scientifically speaking, unsupportable.

 

DMSO has been studied with eight species of mammals, including humans, as well some fish and birds, with almost universal agreement as to its low toxicity. DMSO is roughly 1/7th as toxic as aspirin (http://www.ncbi.nlm.nih.gov/pubmed/17755402)

 

In ABC-TV's Good Morning America's interview of Robert Herschler, the co-discoverer of the pharmaceutical effects of DMSO at 8:17 AM, February 5. 1981, he said, "...the toxicity of DMSO is very low. It's not true that it is dangerous. Compared to aspirin, DMSO is a much safer drug. People are killed taking aspirin; no one has even been killed taking DMSO."

 

If we look at the MSDS for aspirin (https://www.caymanch...sdss/70260m.pdf) it is so toxic that it likely would not be approved by FDA today. Further, it can't be patented, and so FDA would view it, behind closed doors, as competing with patentable drugs too well, as FDA is locked in bed with big pharma.

 

DMSO is an invaluable medicine, which was regarded as one of the three most important medical finds of the 20th century.

 

FDA attempted to ban it in 1965, most likely because it competes with conventional profit-driven medical interventions. This after there were over 300 studies and over 10,000 patient records showing its amazing properties.

 

However, it can't be patented or generate profits AND it can't be put through double-blind studies because of its odd odor. So FDA’s J. Richard Crout, MD, admitted that they could not control its use and thus rejected its approval and then tried to remove it from public use.

 

However, since it is commonly available as an industrial solvent, FDA could not truly ban it.

 

However, FDA ruled that the companies that sell it to those of us that know about it must put warnings on the labels, "Not approved for human use MAY BE UNSAFE." And WARNING: This product is sold as a solvent only. It is unlawful to represent in any way that this product is useful or safe for medical purposes." 

 

Thus, the manufacturer - or, according to FDA, even I am not allowed to talk about DMSO's amazing medical properties. FDA abrogates the First Amendment?

 

It has absolutely changed my life (and continues to), and according to my foot surgeon, has done what is considered to be "medically impossible" in healing torn ligaments in my foot [anterior talofibular (ATF), calcanealfibular (CFL), and posterior talofibula (PTF)] AND my torn Achilles tendon -- without immobilization.

 

Further, I have two friends who experienced chronic migraines, and when DMSO was rubbed on their heads where it hurt, the pain disappeared with five minutes - because DMSO is highly transdermal and blocks conduction in nerves, in a manner similar to the way that local anesthetics work. 

 

Another with chronic sinus headaches keeps a jar of DMSO gel in her purse, because it not only stops her sinus headaches, but stops her carpal tunnel pain, as she is a bookkeeper and working on the computer all day.

https://www.youtube....h?v=MgvymT63oA4

 

DMSO is as important as antibiotics, only antibiotics can be patented and generate great profits.

 

As to DMSO and wrinkles, I have rubbed it on the skin under my eyes with vitamin A, which causes tremendous dryness and wrinkling after a couple days. I worried that I had done permanent damage to the skin.

 

However, on cessation, after about a week, the skin looked as if it had been renewed.

 

Dr. H. Harry Szmant, former Chairman of the University of Detroit's chemistry department said that DMSO "...has a tremendous capacity to dissolve substances. It is a reagent that can speed up some chemical reactions a "billionfold."

 

"The unique capability of DMSO to penetrate living tissues without causing significant damage is probably related to its relatively polar nature, its capacity to accept hydrogen bonds, and its relatively small and compact structure."

 

DMSO penetrates skin to increase the rate of healing of soft tissues beneath skin tremendously. 

 

Thus, it healed the three ligaments in my foot and healed my Achilles tendon by simply rubbing it on the skin above them twice a day for four months.

 

So, yes, it can improve wrinkled skin, too.

 

For more, watch the three 60 minute reports on DMSO on YOUTUBE:

https://www.youtube....h?v=u0i7jARfKeI

https://www.youtube....h?v=icfh4x2vxbA

https://www.youtube....h?v=gvHNN2XbkqU

 

and then various videos at:

https://www.youtube....arch_query=DMSO

Edited by mikey, 10 January 2015 - 09:04 AM.

[Turnbuckle]

 

I still wonder what a little DMSO/c60/OO would do for wrinkles. I know it's used with arnica oil.

 

DMSO is toxic and a mutagen (See MSDS for DMSO)   

 

 

First, this is an off-topic discussion. Second, the MSDS is not a good source of information. It's information is typically yes or no to certain questions, and if you were to read these warnings, you might never use anything. Look at the MSDS for ethanol, for instance. Or even vitamin C, which is described as "Mutagenic for mammalian somatic cells. Mutagenic for bacteria and/or yeast."

 

Here are papers with more in-depth information on DMSO, which suggests it is not benign--

 

Dimethyl sulfoxide (DMSO) is an organic solvent with several biological applications. It is extensively used to dissolve compounds that hardly dissolve in water to detect their genotoxic activity in vitro. In this study DMSO will be tested to determine its genotoxic potential. The effect of DMSO on the frequency of chromosomal aberrations in anaphase as well as DNA fragmentation through the comet assay has been evaluated in the meristematic cells of the root tips of Vicia faba. It has been observed that the frequency of chromosomal aberrations increases when the concentration of DMSO increases, reaching its maximum value with 20% of DMSO and decreasing at 30 and 40% of DMSO, in comparison to this maximum value, but significantly higher than the values observed in the control. Similarly, the percentage of fragmentation and damage index evaluated through the comet assay showed the same behavior; some of the possible mechanisms of action are discussed.

 

http://link.springer...3530-012-0130-9

 

 

 

And another--

 

In the present study, a DMSO concentration of 1% does not significantly affect the astrocyte survival during a 24-h exposure period, but impairs cell viability, mitochondrial integrity and glutamate transporter expression. Exposure of astrocyte cultures to DMSO at concentrations of 5% significantly impairs cell survival and increased apoptosis. Collectively, these results provide strong in vitro evidence for the neuropharmacological and neurotoxicological effects of DMSO.

 

http://www.ncbi.nlm....les/PMC4169574/

 

 

[mikey]

 

 

I still wonder what a little DMSO/c60/OO would do for wrinkles. I know it's used with arnica oil.

 

DMSO is toxic and a mutagen (See MSDS for DMSO)   

 

 

First, this is an off-topic discussion. Second, the MSDS is not a good source of information. It's information is typically yes or no to certain questions, and if you were to read these warnings, you might never use anything. Look at the MSDS for ethanol, for instance. Or even vitamin C, which is described as "Mutagenic for mammalian somatic cells. Mutagenic for bacteria and/or yeast."

 

Here are papers with more in-depth information on DMSO, which suggests it is not benign--

 

Dimethyl sulfoxide (DMSO) is an organic solvent with several biological applications. It is extensively used to dissolve compounds that hardly dissolve in water to detect their genotoxic activity in vitro. In this study DMSO will be tested to determine its genotoxic potential. The effect of DMSO on the frequency of chromosomal aberrations in anaphase as well as DNA fragmentation through the comet assay has been evaluated in the meristematic cells of the root tips of Vicia faba. It has been observed that the frequency of chromosomal aberrations increases when the concentration of DMSO increases, reaching its maximum value with 20% of DMSO and decreasing at 30 and 40% of DMSO, in comparison to this maximum value, but significantly higher than the values observed in the control. Similarly, the percentage of fragmentation and damage index evaluated through the comet assay showed the same behavior; some of the possible mechanisms of action are discussed.

 

http://link.springer...3530-012-0130-9

 

 

 

And another--

 

In the present study, a DMSO concentration of 1% does not significantly affect the astrocyte survival during a 24-h exposure period, but impairs cell viability, mitochondrial integrity and glutamate transporter expression. Exposure of astrocyte cultures to DMSO at concentrations of 5% significantly impairs cell survival and increased apoptosis. Collectively, these results provide strong in vitro evidence for the neuropharmacological and neurotoxicological effects of DMSO.

 

http://www.ncbi.nlm....les/PMC4169574/

 

 

 

 

Those are interesting studies, Turnbuckle.

 

Do you know of similar human in vivo data?

[sensei]

I recently noticed not only the gray disappearing from my beard, but also the color returning to what it was about 15 years ago. 

 

In my mid-late 20's my hair was still blonde, and my beard was an auburn with hints of gold.  Through my 30's and 40's the hair on my head and beard darkened to a dull brownish/dark dark blond.

 

I also have a pronounced scar from a head injury that is approximately 2 cm long, and .5 cm across and went to the bone, above my eye near my orbital socket.  It seems to be getting less pronounced.

 

I will see if I have any pictures for comparison (of the scar)

 

 

[Kalliste]

The only effect I feel certain about so far is less need for sleep. I woke up 4 in the morning today despite having turned in at 10pm after a big dinner, some wine and another little thing that makes you sleepy and hungry... I took 4 ml before going to sleep. I'm going to continue varying the dosage to see what happens,

[pone11]

I recently noticed not only the gray disappearing from my beard, but also the color returning to what it was about 15 years ago. 

 

In my mid-late 20's my hair was still blonde, and my beard was an auburn with hints of gold.  Through my 30's and 40's the hair on my head and beard darkened to a dull brownish/dark dark blond.

 

I also have a pronounced scar from a head injury that is approximately 2 cm long, and .5 cm across and went to the bone, above my eye near my orbital socket.  It seems to be getting less pronounced.

 

I will see if I have any pictures for comparison (of the scar)

 

It would be really interesting to see photos of the color changes in the beard as well.

 

It's a shame that people are not doing measurements of oxidation related metabolites before they start C60, so that we can get real data on what is changing in the system.   I think it would be valuable to have direct measurements of oxidative damage like lipid peroxides, before and after.

 

Is there any blood test we can do that would directly measure the level of oxidative stress?  Measuring just the impact of the stress is not enough, because it could be the oxidative stress remains at a high level, and we simply compensated for this by raising antioxidants (e.g., glutathione peroxidase, SOD, or catalase).