6219 replies to this topic

[Strangelove]

You have to give NSI-189 a full 28 days of dosing to get its benefits.

Anyone getting effects from the first few days is simply the placebo effect. Though, i could be wrong but this is my personal experience from multiple cycles.

I keep a diary of when NSI-189 kicks in, and its obvious. Its always around the day 22 mark.

 

This is not always the case, but everyone trying it should persist for at least month, I remember a member (maybe Major Legend?) that had its first effects after one and a half months. I got manic with my first NSI-189 dose in a way I never expected, it had the same effect (to a lower degree) for the following 3-4 days until it subsided to a more natural positive feeling. I have a pm from a guy in UK with the exact same experience. He said that possibly is going to post it in the future, so he maybe do so if/when he read this. I do not think there is another experience like his posted on line, extreme good feelings (close to manic) I ll maybe contact him to see if its OK to copy/paste his pm.

[drg]

Hey SL I have been trying to figure it out. What application is HPLC best for and what applications should GC-MS be used?

[Strangelove]

Hey SL I have been trying to figure it out. What application is HPLC best for and what applications should GC-MS be used?

 

Both HPLC and GC have the same function in separating the compounds of a chemical mixture. From previous reading on line choosing the best method to analyze NSI-189, HPLC is the most straightforward way for quantification of the components (if more than one) of a mixture to check purity, but you can also do this with GC with some extra step.

 

MS is added in GC to identify a chemical with great accuracy comparing with the spectrums in a database or directly trying to figure the unknown chemical through the total mass indicated, elements present and some other characteristics I do not remember right now. I guess you are asking for analyzing mifepristone samples.

 

I do not know what are the price ranges for GC-MS, but for identification pm me to give you a lab for NMR spectroscopy for $200. Although as mifepristone is not very expensive we could try to subjectively see if our experiences match reports online and test only for purity, saving money.

 

Edit: If what do you ask is, if you can identify mifepristone with HPLC only, I do not think so.

Edited by Strangelove, 09 December 2015 - 04:36 PM.

[SearchingForAnswers]

http://medicalxpress...tive-phase.html

 

I don't know if this is new info (I had to hand type the link in, might be errors), but it's dated 8 December and it's regarding NSI-189.

 

Edited by SearchingForAnswers, 14 December 2015 - 06:36 AM.

[Mind_Paralysis]

Links not working, SearchingForAnswers.

[SearchingForAnswers]

Strange, I edited it correctly.

 

http://medicalxpress...tive-phase.html

 

 

 

[panel]

Nice article. Thanks a lot

 

So this confirms:

1. NSI-189 shows improved both depressive and cognitive symptoms

2. Effects appear to persist for several months after treatment discontinuation

3. Research: 3 groups. 8 patients each (2 got placebo, 6 got NSI-189 dosage in different dosages for each group 1x40mg, 2x,40mg, 3x40mg). Reports of adverse effects - none of them serious - were similar in both the control group and in participants receiving the active drug, even those who took the maximum daily dose.

4. EEG readings taken prior to and at several times during treatment showed some changes, primarily an increase in high-frequency alpha waves, and MRI scans suggested the possibility of increased hippocampal volume in those taking the active drug.

5. On four measures of depressive or cognitive symptoms, participants receiving the active drugs showed improvement after the 28-day treatment period, with significant differences from the placebo group on two measures based on participants' self-reports. Symptom improvement was maintained throughout the follow-up period (the effects of current antidepressants typically only last as long as they are taken)

 

BWT larger phase 2 trial of NSI-189 that has recently been initiated

 

 

[Mind_Paralysis]

Strange, I edited it correctly.

 

http://medicalxpress...tive-phase.html

 

It's working now - cheers mate! =)

[Sleepdealer]

I wonder how much the growth of the hippocampus and amygdala would continue on a longer trial, like how much and how long. On one month the effects seemed efficient but the growth were statistically insignificant, right? Maybe it would be markably significant after 3-6 months of the same daily dosage.

Edited by Sleepdealer, 15 December 2015 - 10:06 PM.

[panel]

I wonder how much the growth of the hippocampus and amygdala would continue on a longer trial, like how much and how long. On one month the effects seemed efficient but the growth were statistically insignificant, right? Maybe it would be markably significant after 3-6 months of the same daily dosage.

 

Mouse trial

+20% in 1 year

[chrisp2]

Mouse trial

+20% in 1 year

 

 

Phosphate...  Oral delivery?

[Lsdium]

 

I wonder how much the growth of the hippocampus and amygdala would continue on a longer trial, like how much and how long. On one month the effects seemed efficient but the growth were statistically insignificant, right? Maybe it would be markably significant after 3-6 months of the same daily dosage.

 

Mouse trial

+20% in 1 year

 

 

Got any Links for that?

[panel]

 

 

I wonder how much the growth of the hippocampus and amygdala would continue on a longer trial, like how much and how long. On one month the effects seemed efficient but the growth were statistically insignificant, right? Maybe it would be markably significant after 3-6 months of the same daily dosage.

 

Mouse trial

+20% in 1 year

 

 

Got any Links for that?

 

 

I do not have it here, but I have watched video (fromsome medical conference) and president of Neuralsteam (company testing NSI-189) talked about this reseach.

 

You could review this long thread to find this video or simply try to google it (NSI-189, hipocamp 20%, mouse) and you'll definitly find a relevant source of this research.

 

This is a quote from my notes:

"Studies have shown that it stimulates neurogenesis of human hippocampus-derived neural stem cells in vivo and vitro (5). In healthy adult mice, NSI-189 has been shown to increase the hippocampal volume by 20% (due to synaptogenesis) and reverse behavioral symptoms in mouse depression models, meaning at the source it could address depression (5). "

[finalgates]

can you use it while on ssris?

[bocor]

I have yet to try nsi but am wandering anyone have any help with PTsd by taking it? I have multiple ptsd issues which causes a lot of paranoia the only thing that concerns me is radically this stuff seems to grow brain tissue couldn't this contribute to cancer somehow?

[Mind_Paralysis]

I have yet to try nsi but am wandering anyone have any help with PTsd by taking it? I have multiple ptsd issues which causes a lot of paranoia the only thing that concerns me is radically this stuff seems to grow brain tissue couldn't this contribute to cancer somehow?

 

I think we're way past that point now - they've actually begun human testing, which has gone fairly well. They're doing a phase 2 trial now. The reason they've gone into human testing is of course because none of the test-animals developed cancer or any other serious side-effects.

If it passes that trial as well, then I would say the risk is negligible - you'll be more likely to contract brain-cancer from your detergent.

 

Your fear is logical and well-placed, a reasonable assumption, but it has become much less likely with the newer data.
 

[bocor]

Much thx for info I'm seriously considering it even with risk as I have really bad ptsd

[justabody]

So what's the consensus on the ROA? Neuralstem states oral in the phase 1b results, but couldn't oral mean sublingual? I feel stuck between risking low bioavailability (oral) and missing the bell-shaped curve because I'm absorbing too much sublingually. Basically, what are the success stories' ROAs?

Edited by justabody, 18 December 2015 - 01:39 AM.

[Shai Hulud]

No, oral doesn't mean sublingual. They also use the phosphate, which works much better orally than sublingually.
So your chosen ROA would depend on what you can get: If it is freebase, you choose sublingual RoA. If it is phosphate, choose oral RoA.

[Sleepdealer]

 

They also use the phosphate, which works much better orally than sublingually.
So your chosen ROA would depend on what you can get: If it is freebase, you choose sublingual RoA. If it is phosphate, choose oral RoA.

 

Wait, how do we know this? If Strangelove's NSI-189 is phosphate then I've been taking it wrong all the time, I took it sublingually.

[Perek]

SLs NSI is free base, got a neutral taste. I use ut myself sublingual and find it less anxiogenic than the bitter tasting phosphate, taken oral or sublingual.

[panel]

No, oral doesn't mean sublingual. They also use the phosphate, which works much better orally than sublingually.

 

Why do you think so? 

 

 

I am taking NSI sublingual, so now I start to wonder is there really any diifference in this case?

 

Let me explain:

The point of taking something sublingual is to improve it's absorbtion - the substance is abssorbed in mountch and never get's to stomach (or only part of substabnce gets there). 

 

So now we have 2 scenarios of doing it 'wrong':

1. Should be taken sublingual but you take it oral

All substance goes to stomach = worse absorbtion

 

2. Should be taken oral but you take it sublingual (like NSI-189)

Maby part of substance will be absorbed sublingual rest will still go to stomatch with saliva (It's almoust like oral)

Do you thik there is much difference in this case?

[Shai Hulud]

It was mentioned several times in this thread. Don't remember if there was a neuralstem source for it.
Paul from Ceretropic also mentioned it to me.

[justabody]

I'll vouch for the fact it seems to work orally. Also this stuff (phosphate) is hygroscopic and clumps up like nobody's business, unfortunate. It's like Tianeptine.

 

Edited by justabody, 21 December 2015 - 06:11 PM.

[motorcitykid]

I wonder if a possible mechanism of action for NSI-189 is that increases expression of the newly discovered protein Dnmt3a2. Knockdown of protein in the hippocampus enhanced fear memory formation.

 

Researchers hope the discovery of this protein will open the door for new treatments for PTSD.

 

Here's the paper:

http://www.ncbi.nlm....ubmed/26598069/

 

[adamh]

Sorry to ask a dumb question but I can't read 120 pages to see if its been answered before. I see NSI-189 for sale on amazon at $75 for 1.2 gm. There are only 3 reviews but they are all positive. Should I go for this or is there a group buy which is a better deal or a better source that is vouched for?

[aribadabar]

Sorry to ask a dumb question but I can't read 120 pages to see if its been answered before. I see NSI-189 for sale on amazon at $75 for 1.2 gm. There are only 3 reviews but they are all positive. Should I go for this or is there a group buy which is a better deal or a better source that is vouched for?

 

Read the last 10 pages and you will find your answer.

If you can't be bothered to do that, go with Amazon.

[adamh]

ari, thats better than no answer at all. I can read 10 pages. Whats wrong with amazon? Chinese junk? And I don't mean a boat.

[drg]

Strange love is a good guy and like 1/3 the price. I am sure you can find many better sources for that money

[Al Capacino]

I don't think there's much, if any, legit nsi out there these days. My last 3 nsi purchases from different vendors have either non existent effects or massively watered down effects which are hard to tell apart from placebo. Nothing compared to my first two experiences of several months on nsi. Judging by the lack of posts from earlier nsi users as well makes me assume this is true for others too.