The skin reaction was from something else, not from C60. I noted no C60 age reversal with one dog, but quite a bit with the other. The effect lasted about a year and a half before fading, and subsequent doses provided no improvement.
Do you have an theories why there was no subsequent benefits to the administration of c60, say stem cell depletion? Were the dogs particularly old?
I can't draw any conclusions from one dog, but I and several others here have noted a fading of C60 effects with time. The reason remains unknown, but it's easy to speculate that, if C60 is doing something to increase ATP production (either by blocking UCP pores, reducing methylation of mtDNA, acting as a super antioxidant, or dragging grafted polyphenols into the mitochondria with it), and if can stimulate mitochondria in stem cells, then it would wake them up as that is the switch for turning them on. Ultimately, however, the boost from C60 is like the boost from mitochondrial fusion--it increases ATP production but does nothing to cull defective mitochondria, and thus ATP production ultimately declines.
As for stem cells, there may be a sub-population of them that is readily stimulated by C60 and that is used up.
As for the possibility of C60 dragging polyphenols into mitochondria, see this post where I experimented with grafting Coq10 to C60 using red light--
First trials with C60/MCT + CoQ10 + red light:
The mix was a fresh prep of C60 in MCT oil stirred for one week in the dark and not filtered. The C60 was in excess of the solubility limit as some particles remained and the fluid was turbid. To this I added 3mg/ml COQ10 powder and stirred for an hour. This might also be in excess of the solubility limit.
½ teaspoon produced no noticeable exercise benefit at all. Pretty much a zero. The next day I exposed it to 2 minutes of red light as described above, and took another ½ teaspoon. I didn’t think there would be any effect as I didn't give it much light and I didn’t initially feel anything, however I was wrong about that. Anaerobic exercise capacity was noticeably higher than any trial reported above with HT. I haven’t any experience with mitoQ, but I imagine that C60 is acting in a similar manner, dragging CoQ10 adducts into the mitochondria. Later I did get a slight head effect—a slight pressure that was vaguely unpleasant. This could be the result of too much coffee, however.
Years ago I'd added CoQ10 to C60/EVOO and found no benefit, so it appears that irradiation makes all the difference. The CoQ10 has to be attached and red light appears to create reactive sites sufficient to do that.
The best approach, possibly, is to employ a program of forced fission/fusion to restore mitochondria to a healthy state throughout the body, and then use C60 sparingly at the peak of mito health and mito mass. (See Manipulating mitochondrial dynamics.) This is an experiment for the near future.
Edited by Turnbuckle, 14 November 2017 - 11:29 AM.
