4029 replies to this topic

[lostfalco]

joeCohen.JPG

 

Have you thought about moving to Cambria and joining the revolution?

haha Yeah Thomlandia, Joe and I have talked about some ideas. Just thinking through all my options. =)

Edited by lostfalco, 23 February 2016 - 03:23 AM.

[lostfalco]

Hey Falco, I'm sure you have covered this before so I'm sorry.. But I was wondering I read somewhere that targeting the back of your brain with lllt can alter your cercadian rhythm, do you have any comments on this and do you still target your whole brain?
Hey Falco, I'm sure you have covered this before so I'm sorry.. But I was wondering I read somewhere that targeting the back of your brain with lllt can alter your cercadian rhythm, do you have any comments on this and do you still target your whole brain?

Hey Bluecheer, no worries. Yeah, I still laser my whole brain. I don't think there are any circadian concerns. 

Edited by lostfalco, 23 February 2016 - 01:22 AM.

[lostfalco]

I really think a store for unique products like JDTiC, guanfacine, and other worthwhile nootropics are a great thing to start.

 

 

 

As for your latter post on helping people with depression. 

 

For the time being I'm betting my last bet on NRX-1074 after my extensive research in the area of nootropics/depression treatment and cognitive enhancement. Hope this helps in some small way.

 

Cheers.

 

DERP, I totally forgot NSI-189. Lasting positive effects on depression from most of the research I've seen on the topic. Kudos

 

Thanks, yada! Totally appreciate the advice. There are definitely a lot of opportunities to help people. I've read thousands of studies at this point so I have some pretty good ideas about various protocols but I just want to make sure I'm 'doing no harm' first. I'm willing to take bigger risks with myself than I am with others. Anyway, cool you're leading that group buy! Looking forward to reading about people's experiences. 

Edited by lostfalco, 23 February 2016 - 01:46 AM.

[LongLife]

DEPRESSION: If you have not looked into the methylation (epigenetics) related cause of depression, as well as a host of ailments associated, such as ADHD, ADD, Autism, then tale a look at Dr. William Walch and Dr. Ben Lynch websites and YouTube educational and training videos. They offer online classes to practitioners and interested public too. Coupled with personal blood type, see Dr. Peter D'Amado, you get a very different perspective of the cause:effect relationship as opposed to the standard medical view and treatment with drugs and synthetic substances (BigPharma). Reading the forum's threads under "playground" and his perspective concerning Alzheimer's cause:effect and the individual characteristics of biochemistry that people have points out why some do and others do not receive a benefit from nootropics (racetams) which eludes to the body type (blood type) and epigenetic polymorphism and stochastic occurrence.

 

About 60% of the population have mutated genes (polymorphics) and up to 80% of these people suffer from depression or other maladies which can be altered by gene expression "adjustments" through supplementation once identified, not to mention many other current and near epidemic social behavioral problems. View these websites:

 

http://www.walshinst...h-phd-facn.html

http://mthfr.net/about/dr-lynch/

http://www.dadamo.co...t/index.pl?2000

http://www.longecity...ogenic-diet-kd/ AND OTHERS by playground

 

LOSTFALCO, your extensive research for improving memory, all forms of memory I assume, is admirable to say the least and your insatiable quest for knowledge, to retain it and apply it is genuine. In my opinion you will need to have some degree after your name to be recognized as a viable consultant. Upon examination of the the first three web links above, as well as viewing a few YouTube videos by these men, you will appreciate the dire need for continuing education in the professional health care arena, which is extensive and multifaceted. Reminds me of Tony Robbins when he started out coaching. Health care practitioners/providers of all classes could utilize your extensive experience and knowledge base, willing to exchange access to that for some FRN's, obviously. You have a niche market to exploit in memory; enhancement, retention, gain, etc. I know you have mentioned in passing EPIgenetics, so you know it is the future come now and a wide open field. So the answer to your question is GO FOR IT. I think giving talks at colleges and universities would bring you viral for substance sales as well as continuing education on a general level. Obviously person-to-person would not be feasible nor profitable. Serious individuals would need to provide you with their 23andMe genome sequencing, Geno-Type Kit results, blood type and what their desired results are (ie, fix my brain fog, my short term memory, my attention disorder, my insomnia, my lack of energy, etc.), all of which are very DOable. Good fortune in your business venture, the World is at hand young man. You will eventually team up with a medical doctor, a ND or Psychiatrist, certainly.

Edited by LongLife, 28 February 2016 - 02:11 AM.

[Nuke]

Quick update.

 

I'm currently building my Led array. I'm using these Leds.

 

As you can see on the photo, a single string pulls almost 100mA. That should give a radiant power of 50mW per led. This array is planned for 60 leds, on an area of 4cm x 6.5cm. That is 3w of radiant power total or about 115mW per cm2.

 

I'll need some new resistors, the current ones power dissipation are far to low, so they get extremely hot. The Leds are fine though.

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[Sirocco]

Nuke, you could always use more than one resistor connected in parallel with higher resistance (current resistance * amount of parallel resistors) this way you will split power dissipation between multiple elements. Depending on how big you want your array you may be able to get away with that.

[Nuke]

It is what I'm doing for now. I'm using 2x 180 ohm in parallel. Unfortunately I only have 4 left, so I have to make a plan anyway. There is an electronic shop around the corner from the office, so I guess I can go grab some in lunch time tomorrow.

[montana2012]

Hi everyone,

 

So, around 15 months ago I decided to bite the bullet and bought nanoparticle/colloidal (different sized - from atoms up to small colloidal clusters) gold on eBay, for it's supposedly vaunted ability to boost Gf. I don't think it had any positive impact on my IQ, as a matter of fact, my brain felt foggy on it.

 

So, I took it regularly for the first few months. First time I took it I couldn't sleep for 3 nights in a row, I even had a bottle of vodka on one of the nights in an attempt to pass out - didn't work. So, it's not enhancing cognition, it dumbs one down slightly, but the energy/wakefulness boosting effects are spectacular. It also ups the libido significantly.

 

After the initial few months, there was a big hiatus of around 6 months where I didn't touch it at all, until lately... since wellbutrin started making me sleepy on my 4th day on it... 

 

The reason I took a break from the gold wasn't the ruined sleep pattern... it was the pain I felt in the liver area (under my right rib, may not be the liver necessarily) each time I took it. Never checked my liver enzymes while on it, I might do it this week for sports.

 

Many people claim gold shouldn't react with anything in your body, since it's gold, duh... but there's a definite effect. And now I wonder about the other potential side effects, apart from the brain fog.

 

So, is anyone else experienced with this? What is the long-term accumulation/toxicity potential of this thing? I'm including some studies in as well:

Even though the collective results in the literature show controversy about the toxicity of gold nanoparticles, we think that the uptake and toxicity of these nanomaterials are controllable and can be manipulated. As discussed earlier in this perspective, researchers have demonstrated the ability to “detoxify” and regulate the uptake of these nanoparticles by functionalizing the surface of the nanoparticles with smart/benign ligands. In an encouraging recent example, one single intravenous injection of PEG-functionalized gold nanorods which showed a long circulating time (t1/2 17 h) was able to destroy human xenogaft tumors in mice (von Maltzahn et al. 2009). In this study, the total dose of nanorods required for complete photothermal destruction of the tumor was 20 mg/kg (in gold atoms) and did not induce any toxicity in tumor-free mice (von Maltzahn et al.2009). As more data are collected, we are optimistic that proper attention will be paid to surface chemistry and dosages, so that the full potential of gold nanoparticles for biomedical applications can be exploited.

 

Along with the number of potential applications for gold nanoparticles (AuNP) especially for medical and scientific purposes, the interest in possible toxic effects of such particles is rising. The general perception views nanosized gold colloids as relatively inert towards biological systems. However, a closer analysis of pertinent studies reveals a more complex picture. While the chemical compound of which the nanoparticles consists plays an important role, further biocompatibility determining aspects have been made out. The vast majority of trials concerning AuNP-toxicity were performed using somatic cell culture lines. The results show a considerable dependency of toxic effects on size, zeta potential and surface functionalisation. In vivo studies on this subject are still rare. Based on the existing data it can be assumed, that a dosage of under <400 µg Au/kg showed no untoward effects. If higher amounts were applied toxicity depended on route of administration and particle size. Since nanoparticles have been shown to cross reproduction-relevant biological barriers such as the blood-testicle and the placental barrier the question of their reprotoxicity arises. Yet data concerning this subject is far from adequate. Regarding gametes, recent experiments showed a dose-dependent sensitivity of spermatozoa towards AuNP. Oocytes have not yet been tested in that respect. Interestingly, so far no effects were detected on embryos after gold nanoparticle exposure. In conclusion, the biocompatibility of gold nanoparticles depends on a range of particle specific aspects as well as the choice of target tissue. Further clarification of such matters are subject to ongoing research.

Edited by montana2012, 28 February 2016 - 12:01 PM.

[medievil]

I just started reading this thread and im a big fan, you remind me of me in ways.

[lostfalco]

DEPRESSION: If you have not looked into the methylation (epigenetics) related cause of depression...

 

LOSTFALCO, your extensive research for improving memory, all forms of memory I assume, is admirable to say the least...

Thanks for the encouraging words, LongLife! I've studied epigenetics pretty extensively and posted quite a bit about it in this thread in 2014. I totally agree with you that it holds a huge amount of promise for treating a myriad of ailments. There are so many exciting discoveries coming. I appreciate the business ideas as well. You've given me a lot of good things to think about. I think I'm leaning a bit towards the educational side at the moment and seeing how that goes before setting up the infrastructure required to create my own products. It's a tough call though because there are a number of supplements that don't currently exist that I would love to create. We'll see...

Quick update.

 

I'm currently building my Led array. I'm using these Leds.

 

As you can see on the photo, a single string pulls almost 100mA. That should give a radiant power of 50mW per led. This array is planned for 60 leds, on an area of 4cm x 6.5cm. That is 3w of radiant power total or about 115mW per cm2.

 

I'll need some new resistors, the current ones power dissipation are far to low, so they get extremely hot. The Leds are fine though.

Looking good, Nuke! Did you end up getting 830nm LEDs?

[lostfalco]

Hi everyone,

 

So, around 15 months ago I decided to bite the bullet and bought nanoparticle/colloidal (different sized - from atoms up to small colloidal clusters) gold on eBay, for it's supposedly vaunted ability to boost Gf. I don't think it had any positive impact on my IQ, as a matter of fact, my brain felt foggy on it.

 

Sorry montana, I haven't researched gold nanoparticles to any significant extent. Maybe someone else can chime in. 

[lostfalco]

I just started reading this thread and im a big fan, you remind me of me in ways.

Thanks, medieval. I appreciate it. I've definitely enjoyed your posts over the years as well. What are you currently experimenting with?

[medievil]

My current regime is:

 

NSI

Nefiracetam

Oxiracetam

 

but i ran out of those because of money issues but they should be part of it

 

3 fluorophenmetrazepime or something like that, its a cross btw amp and oxycodone but pretty much just a stim

4 fluoroethylphenidate

1p lsd in treshold doses

2 cannabinoids i have i cant remember the name off

codeine

clonazepam

phenibut

lexapro

 

im experimenting with diff rewarding substances as they enhance reward in diff ways, stims for me is the baseline and takes me away from anhedonia torture, a state where no other rewarding substance besides stims work, indicating total dysfunctioning of the reward system which causes extreme suffering, also olanzapine made me find sports entertaining and obsessed with top gear while i normally dont like sports or cars, another way how you can induce reward by a differened mechanism.

 

i would like to try alot more differened things and just have a dex script for during the week so i dont have to abuse rc stims during the week leaving me without many, i wonna keep recreational doses for the weekend, also the combo of my stims with 1p lsd and a cannabinoid causes extreme arrythemia and weird feeling in my heart, i had to call the ambulance once as i was concerned about long qt syndrome or getting cardiac arrest, those symptions would allways induce a panic attack but the scan in the ambulance made them only concerned about my fast heartbeat so i refused to go to hospital as i know i didnt have prolonged qt or anything that can cause severe issues.

 

anyway im holding off that experiment for a bit, also tried mxp its like ketamine but i didnt like it much and much prefer the racetams which i all wonna try, eventually perhaps combine most of them as they all have unique effects, you know i can take things to an extreme.

 

but anyway i cant afford enough stims and allways collapse in severe anhedonia which is nearly impossible to endure, then i also stop taking my sups which can interfere with getting all the benefits, once im back in belguim for a bit and get dexamphetamine things should be better.

 

Also didnt have psychosis or psychosis like symptions for 3 years now, while usually induced by stims im doing much better, also with regards with my cognitive thinking as i sounded crazy at times, ill allways do crazy experiments tough

[bigyellowlemon]

So clonidine is effective, just not as effective as guanfacine?  Why is that? Clonidine effects more adrenic receptors more strongly. Maybe the selecitve action of guanfacine is the key. 

 

https://en.wikipedia...anism_of_action

https://en.wikipedia...ne#Pharmacology

[medievil]

I type too fast at times, its hard to read my above post lol, but i also do this on therapeutic dex doses i live life in the fast line, ive allways been hyper

[lostfalco]

Intranasal insulin enhances brain utilization of glucose and is a VERY intriguing treatment possibility for treating concussions/CTE/PTSD as well as Alzheimer's. Check out this YouTube vid for a quick summary of info (watch 2x speed!). 

 

Novolin R is $25 at Walmart in the U.S. without a prescription. I've been taking it for almost two months now and it's WAY up on my list of favorite noots. I've noticed enhanced brain energy and massive mood improvements (even though I'm normally pretty happy to begin with!). 

 

Edited by lostfalco, 05 March 2016 - 07:41 PM.

[Remington]

Intranasal insulin enhances brain utilization of glucose and is a VERY intriguing treatment possibility for treating concussions/CTE/PTSD as well as Alzheimer's. Check out this YouTube vid for a quick summary of info (watch 2x speed!).

Novolin R is $25 at Walmart in the U.S. without a prescription. I've been taking it for almost two months now and it's WAY up on my list of favorite noots. I've noticed enhanced brain energy and massive mood improvements (even though I'm normally pretty happy to begin with!).

https://www.youtube....h?v=g-fVO0tobFw

Hey lostfalco, I ran out of my Novolin but what affects have you felt? Just want to compare experiences. I've been using the Novolin N though, does it matter if it's not the R version?

[lostfalco]

 

Hey lostfalco, I ran out of my Novolin but what affects have you felt? Just want to compare experiences. I've been using the Novolin N though, does it matter if it's not the R version?

 

Hey Remington, the N is longer lasting than R but it shouldn't matter too much (though maybe a little). I've noticed mood and energy effects. What about you? How has your experience been so far?

[Remington]

Hey lostfalco, I ran out of my Novolin but what affects have you felt? Just want to compare experiences. I've been using the Novolin N though, does it matter if it's not the R version?

Hey Remington, the N is longer lasting than R but it shouldn't matter too much (though maybe a little). I've noticed mood and energy effects. What about you? How has your experience been so far?

It's kinda of tough to tell on mood and energy for me since I'm always in a good mood most days and also I keep with exercise.

But I thought I noticed an increase in information retaining for a moment. I couldn't tell if it was placebo or not so that's why I took a break and maybe I can start over and see if I get the same affect just to be sure.

Did you notice anything with longterm memory or has that never been a problem with you?

[lostfalco]

 

It's kinda of tough to tell on mood and energy for me since I'm always in a good mood most days and also I keep with exercise.

But I thought I noticed an increase in information retaining for a moment. I couldn't tell if it was placebo or not so that's why I took a break and maybe I can start over and see if I get the same affect just to be sure.

Did you notice anything with longterm memory or has that never been a problem with you?

 

Thanks for the feedback! Long term memory is a bit tough to tell without pre-testing, post-testing, and blinding. My Anki studying has been excellent while on insulin as has my schoolwork but I can't say anything very definitive. My time is ridiculously limited at the moment (3 jobs and school).

 

I asked gwern to perform a blinded experiment with it and he seemed pretty interested in the idea...but gwern's gonna do what gwern's gonna do. I'll let everyone know if he decides to do it. Fingers crossed. If anyone else has tried intranasal insulin please jump in and let us know your experience (good or bad)! 

Edited by lostfalco, 05 March 2016 - 11:15 PM.

[lostfalco]

Upcoming experiment: intranasal thymosin beta 4. 

 

In the spirit of snorting every bodybuilding substance I can find, I've decided to give intranasal thymosin beta 4 a go and see what happens. As far as I know I'll be the first to try this but I'm sure some crazy bodybuilder has accidentally snorted lines of it while in a drunken stupor.

 

There's some info on it below if anyone wants to check it out. It lowers inflammation and increases neurogenesis, angiogenesis, oligodendrogenesis, and axonal remodeling but the exact mechanism is not precisely known. It is especially associated with myelinogenesis and appears to be an excellent candidate for demyelinating disorders like Multiple Sclerosis (MS). 

 

TB4 downregulates inhibitors of the PI3/Akt pathway and therefore should synergize well with PI3/Akt enhancers like intranasal insulin, IGF-1, dihexa, 7,8 dihydroxyflavone, etc. 

 

Note: VERY high risk

 

http://www.ncbi.nlm....cles/PMC3471669

 

Ann N Y Acad Sci. 2012 Oct;1269:110-6. doi: 10.1111/j.1749-6632.2012.06651.x.

Treatment of neurological injury with thymosin β4.

Morris DC1, Zhang ZG, Zhang J, Xiong Y, Zhang L, Chopp M.

Author information

Abstract

Neurorestorative therapy targets multiple types of parenchymal cells in the intact tissue of injured brain tissue to increase neurogenesis, angiogenesis, oligodendrogenesis, and axonal remodeling during recovery from neurological injury. In our laboratory, we tested thymosin β4 (Tβ4) as a neurorestorative agent to treat models of neurological injury. This review discusses our results demonstrating that Tβ4 improves neurological functional outcome in a rat model of embolic stroke, a mouse model of multiple sclerosis, and a rat model of traumatic brain injury. Tβ4 is a pleiotropic peptide exhibiting many actions in several different types of tissues. One mechanism associated with improvement of neurological improvement from Tβ4 treatment is oligodendrogenesis involving the differentiation of oligodendrocyte progenitor cells to mature myelin-secreting oligodendrocytes. Moreover, our preclinical data provide a basis for movement of Tβ4 into clinical trials for treatment of these devastating neurological diseases and injuries.

 

"The three models of neurological injury treated with Tβ4 described in this review support our hypothesis that Tβ4 is a potential neurorestorative agent. Neurorestorative agents target intact parenchymal cells to promote brain remodeling or repair of damaged tissues. Improvement of neurological functional outcome observed in all three models is associated with oligodendrogenesis and/or the differentiation of OPC into mature OL. Tβ4 could, in theory, treat all three diseases, stroke, multiple sclerosis, and TBI. Presently, research is focusing on optimizing the dose and the time to administer the peptide after injury. These preclinical studies suggest that clinical trials are warranted for Tβ4 treatment of these debilitating diseases in humans."

 

 

http://www.ncbi.nlm....pubmed/26466330

 

J Neurochem. 2016 Jan;136(1):118-32. doi: 10.1111/jnc.13394. Epub 2015 Nov 10.

Thymosin beta 4 up-regulates miR-200a expression and induces differentiation and survival of rat brainprogenitor cells.

Santra M1, Chopp M1,2, Santra S1, Nallani A1, Vyas S1, Zhang ZG1, Morris DC3.

Author information

 

Abstract

Thymosin beta 4 (Tβ4), a secreted 43 amino acid peptide, promotes oligodendrogenesis, and improves neurological outcome in rat models of neurologic injury. We demonstrated that exogenous Tβ4 treatment up-regulated the expression of the miR-200a in vitro in rat brain progenitor cells and in vivo in the peri-infarct area of rats subjected to middle cerebral artery occlusion (MCAO). The up-regulation of miR-200a down-regulated the expression of the following targets in vitro and in vivo models: (i) growth factor receptor-bound protein 2 (Grb2), an adaptor protein involved in epidermal growth factor receptor (EGFR)/Grb2/Ras/MEK/ERK1/c-Jun signaling pathway, which negatively regulates the expression of myelin basic protein (MBP), a marker of mature oligodendrocyte; (ii) ERRFI-1/Mig-6, an endogenous potent kinase inhibitor of EGFR, which resulted in activation/phosphorylation of EGFR; (iii) friend of GATA 2, and phosphatase and tensin homolog deleted in chromosome 10 (PTEN), which are potent inhibitors of the phosphatidylinositol-3-kinase (PI3K)/AKT signaling pathway, and resulted in marked activation of AKT; and (iv) transcription factor, p53, which induces pro-apoptotic genes, and possibly reduced apoptosis of the progenitor cells subjected to oxygen glucose deprivation (OGD). Anti-miR-200a transfection reversed all the effects of Tβ4 treatment in vitro. Thus, Tβ4 up-regulated MBP synthesis, and inhibited OGD-induced apoptosis in a novel miR-200a dependent EGFR signaling pathway. Our findings of miR-200a-mediated protection of progenitor cells may provide a new therapeutic importance for the treatment of neurologic injury. Tβ4-induced micro-RNA-200a (miR-200a) regulates EGFR signaling pathways for MBP synthesis and apoptosis: up-regulation of miR-200a after Tβ4 treatment, increases MBP synthesis after targeting Grb2 and thereby inactivating c-Jun from inhibition of MBP synthesis; and also inhibits OGD-mediated apoptosis after targeting EGFR inhibitor (Mig-6), PI3K inhibitors (FOG2 and Pten) and an inducer (p53) of pro-apoptotic genes, for AKT activation and down-regulation of p53. These findings may contribute the therapeutic benefits for stroke and other neuronal diseases associated with demyelination disorders.

 

http://www.ncbi.nlm....pubmed/25613458

Expert Opin Biol Ther. 2015;15 Suppl 1:S9-12. doi: 10.1517/14712598.2015.1005596. Epub 2015 Jan 22.

Thymosin β4 as a restorative/regenerative therapy for neurological injury and neurodegenerative diseases.

Chopp M1, Zhang ZG.

Author information

 

Abstract

Thymosin β4 (Tβ4) promotes CNS and peripheral nervous system (PNS) plasticity and neurovascular remodeling leading to neurological recovery in a range of neurological diseases. Treatment of neural injury and neurodegenerative disease 24 h or more post-injury and disease onset with Tβ4 enhances angiogenesis, neurogenesis, neurite and axonal outgrowth, and oligodendrogenesis, and thereby, significantly improves functional and behavioral outcomes. We propose that oligodendrogenesis is a common link by which Tβ4 promotes recovery after neural injury and neurodegenerative disease. The ability to target many diverse restorative processes via multiple molecular pathways that drive oligodendrogenesis and neurovascular remodeling may be mediated by the ability of Tβ4 to alter cellular expression of microRNAs (miRNAs). However, further investigations on the essential role of miRNAs in regulating protein expression and the remarkable exosomal intercellular communication network via exosomes will likely provide insight into mechanisms of action and means to amplify the therapeutic effects of Tβ4.

 

http://www.ncbi.nlm....pubmed/26805386

Neurobiol Dis. 2016 Apr;88:85-95. doi: 10.1016/j.nbd.2016.01.010. Epub 2016 Jan 12.

Thymosin beta4 promotes oligodendrogenesis in the demyelinating central nervous system.

Zhang J1, Zhang ZG2, Li Y2, Lu M3, Zhang Y2, Elias SB2, Chopp M4.

Author information

Abstract

Multiple sclerosis (MS) is a demyelinating disease of the central nervous system (CNS). No effective remyelination therapies are in use. We hypothesized that thymosin beta4 (Tβ4) is an effective remyelination treatment by promoting differentiation of oligodendrocyte progenitor cells (OPCs), and that the epidermal growth factor receptor (EGFR) signaling pathway contributes to this process. Two demyelination animal models were employed in this study: 1) experimental autoimmune encephalomyelitis (EAE), an animal model of MS. EAE mice were treated daily for 30days, with Tβ4 or saline treatment initiated on the day of EAE onset; and 2) cuprizone diet model, a non-inflammatory demyelination model. The mice were treated daily for 4weeks with Tβ4 or saline after fed a cuprizone diet for 5weeks. Immunofluorescent staining and Western blot were performed to measure the differentiation of OPCs, myelin and axons, respectively. To obtain insight into mechanisms of action, the expression and activation of the EGFR pathway was measured. AG1478, an EGFR inhibitor, was employed in a loss-of-function study. Data revealed that animals in both demyelination models exhibited significant reduction of myelin basic protein (MBP(+)) levels and CNPase(+) oligodendrocytes. Treatment of EAE mice with Tβ4 significantly improved neurological outcome. Double immunofluorescent staining showed that Tβ4 significantly increased the number of newly generated oligodendrocytes identified by BrdU(+)/CNPase(+) cells and MBP(+) mature oligodendrocytes, and reduced axonal damage in the EAE mice compared with the saline treatment. The newly generated mature oligodendrocytes remyelinated axons, and the increased mature oligodendrocytes significantly correlated with functional improvement (r=0.73, p<0.05). Western blot analysis revealed that Tβ4 treatment increased expression and activation of the EGFR pathway. In the cuprizone demyelination model, Tβ4 treatment was confirmed that significantly increased OPC differentiation and remyelination, and increased the expression of EGFR and activated the EGFR pathway in the demyelinating corpus callosum. In cultured OPCs, blockage of the activation of the EGFR pathway with AG1478 abolished the Tβ4-increased OPC differentiation. Collectively, these findings indicate that: 1) Tβ4 increases proliferation of OPCs and the maturation of OPCs to myelinating oligodendrocytes which in concert, likely contribute to the beneficial effect of Tβ4 on EAE, 2) EGFR upregulated and activated by Tβ4 may mediate the process of OPC differentiation, and 3) Tβ4 could potentially be developed as a therapy for MS patients, and for other demyelinating neurological disorders.

 

http://www.ncbi.nlm....pubmed/23050817

Ann N Y Acad Sci. 2012 Oct;1270:51-8. doi: 10.1111/j.1749-6632.2012.06683.x.

Neuroprotective and neurorestorative effects of thymosin β4 treatment following experimental traumatic braininjury.

Xiong Y1, Mahmood A, Meng Y, Zhang Y, Zhang ZG, Morris DC, Chopp M.

Author information

Abstract

Traumatic brain injury (TBI) remains a leading cause of mortality and morbidity worldwide. No effective pharmacological treatments are available for TBI because all phase II/III TBI clinical trials have failed. This highlights a compelling need to develop effective treatments for TBI. Endogenous neurorestoration occurs in the brain after TBI, including angiogenesis, neurogenesis, synaptogenesis, oligodendrogenesis, and axonal remodeling, which may be associated with spontaneous functional recovery after TBI. However, the endogenous neurorestoration following TBI is limited. Treatments amplifying these neurorestorative processes may promote functional recovery after TBI. Thymosin beta 4 (Tβ4) is the major G-actin-sequestering molecule in eukaryotic cells. In addition, Tβ4 has other properties including antiapoptosis and anti-inflammation, promotion of angiogenesis, wound healing, stem/progenitor cell differentiation, and cell migration and survival, which provide the scientific foundation for the corneal, dermal, and cardiac wound repair multicenter clinical trials. Here, we describe Tβ4 as a neuroprotective and neurorestorative candidate for treatment of TBI.

 

Edited by lostfalco, 06 March 2016 - 04:08 AM.

[Nuke]

Showing off my LLLT prototype. It gets quite hot and is not the easiest to hold, but it will tell me if I should invest time and money in a better system. I did my first treatment 6pm yesterday, 30s per spot. Usually I like my long hair, but getting the device in my scalp is a PITA.

 

While it may be placebo, I was yawning the whole evening and I ended up going to sleep at 10pm. That is after taking an afternoon nap yesterday, so perhaps something is happening. 

 

I'll stay away from the longer acting insulins. Agonizing the insulin receptor for longer periods may increase resistance in the long term. And I believe most of us is in it for the long run.

 

Btw Falco, have you looked at IGF-2 yet? I find it more interesting that IGF-1.

http://www.nature.co...ature09667.html

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[lostfalco]

Showing off my LLLT prototype. It gets quite hot and is not the easiest to hold, but it will tell me if I should invest time and money in a better system. I did my first treatment 6pm yesterday, 30s per spot. Usually I like my long hair, but getting the device in my scalp is a PITA.

 

While it may be placebo, I was yawning the whole evening and I ended up going to sleep at 10pm. That is after taking an afternoon nap yesterday, so perhaps something is happening. 

 

I'll stay away from the longer acting insulins. Agonizing the insulin receptor for longer periods may increase resistance in the long term. And I believe most of us is in it for the long run.

 

Btw Falco, have you looked at IGF-2 yet? I find it more interesting that IGF-1.

http://www.nature.co...ature09667.html

Very cool, Nuke. I hope your prototype works well for you. Hair is kind of a bitch with LLLT. ha I've toyed with shaving my head a number of times. Still might do it. 

 

There's a trial testing long term intranasal insulin in humans that should have just finished in Feb. Still waiting for results but they are testing your exact question. The longest I've seen in humans so far is around four months and that actually enhanced functional outcome in the patients even months after stopping treatment. I could easily envision cycling it long term as well but the cycle times would be a bit of guesswork at the moment. 8 weeks on, 8 weeks off? Not sure, but a thought I've had especially if the effects last months after stopping. 

 

I have looked into IGF-2 but I haven't found anywhere to purchase it yet. Any ideas? I've tried intranasal IGF-1 but I'm not sure how I feel about it in the short term and I'm VERY cautious about it in the long term. More experiments needed. 

[Nuke]

Peprotech IGF-2. They have some really interesting products, but I have not used them yet. I want to order some NGF and I may try them.

 

I try to watch videos at 2x speed, but it becomes really hard on my ears. The best I can manage is 1.5x, in some videos only 1.25x.

[lostfalco]

Peprotech IGF-2. They have some really interesting products, but I have not used them yet. I want to order some NGF and I may try them.

 

I try to watch videos at 2x speed, but it becomes really hard on my ears. The best I can manage is 1.5x, in some videos only 1.25x.

Those prices are just a bit out of my range. ha  

 

IGF-1 was pretty expensive for me and it's only $66.95 at superior. https://www.superior...m/igf-1-long-r3

 

Btw, the code jj45 gives anyone 45% off at superior. I don't know who jj is but the code works if anyone wants to use it. No affiliation. Makes everything they sell pretty affordable. 

Edited by lostfalco, 06 March 2016 - 05:42 PM.

[Yume]

I've only used a few nootropics before, including LLLT. After reading the studies and watching that video I decided to try the intranasal insulin, so I bought some Novolin R today. It just came in a vial for intravenous use but I'm hoping that I can still use it as an intranasal spray. I'm planning to buy one of these to use with it http://www.mountains...CFdgRgQodQcUJUg. Going to use 10 IU since that's what seems to be used in most studies. Anything I should know Lostfalco?

[cougar]

Hi lostfalco, thank you very much for introducing the intranasal insulin to us. A few weeks ago I asked Walmart about whether insulin was available over the counter here in Canada. I was surprised to hear that it was! However, as the guy asked me which brand I wanted, I had no idea about it. He then told me they had more than eighty different kinds, and said he was not comfortable selling it to somebody who knew nothing about it, and asked me to get a script from my doctor, lol!

Since then I put this aside because I didn't even have time to ask it here. Fortunately I've seen that you've just mentioned the brand to be "Novolin R". So I've decided to move forward. But this time I don't want to show up again like knowing nothing about it. Therefore, I would appreciate it if you could give me more information as to how I should answer their question. For example, how do I answer the question about how many do I need? I mean, what is the unit to buy, in ml, or in vial? What to say if they ask me about how I'm going to use it? Do I just tell them I'm going to inject it? Also, where to buy the nasal spray, and how to answer the question if they ask me what I'm going to use the nasal spray for?

Sorry for asking the stupid questions, I probably should have done some search but I'm just too lazy, which is probably why I needed to try this nasal insulin in the first place, to hopefully improve my mental energy! I'm toooooooo tired lol!

Edited by cougar, 07 March 2016 - 12:11 AM.

[Remington]

Hi lostfalco, thank you very much for introducing the intranasal insulin to us. A few weeks ago I asked Walmart about whether insulin was available over the counter here in Canada. I was surprised to hear that it was! However, as the guy asked me which brand I wanted, I had no idea about it. He then told me they had more than eighty different kinds, and said he was not comfortable selling it to somebody who knew nothing about it, and asked me to get a script from my doctor, lol!

Since then I put this aside because I didn't even have time to ask it here. Fortunately I've seen that you've just mentioned the brand to be "Novolin R". So I've decided to move forward. But this time I don't want to show up again like knowing nothing about it. Therefore, I would appreciate it if you could give me more information as to how I should answer their question. For example, how do I answer the question about how many do I need? I mean, what is the unit to buy, in ml, or in vial? What to say if they ask me about how I'm going to use it? Do I just tell them I'm going to inject it? Also, where to buy the nasal spray, and how to answer the question if they ask me what I'm going to use the nasal spray for?

Sorry for asking the stupid questions, I probably should have done some search but I'm just too lazy, which is probably why I needed to try this nasal insulin in the first place, to hopefully improve my mental energy! I'm toooooooo tired lol!

Hey, cougar I kind of went through the same thing when picking it up from walmart, I just told them I was picking it up for my father. Or just bypass the questions all together and have someone else pick it up for you. As long as you don't ask questions or look like you don't know what your purchasing they won't care.

[cougar]

Hey Remington! Thanks for sharing, very helpful. But I guess I will still have to answer the question of how many I need to buy. What's the unit, for example, 1000 ml or 100 vial? If I say I want Novolin R, are they going to ask me more detailed question, for example Novolin R alpha, or Novolin R beta etc? What if they don't have Novolin R? I was amazed at how they've made a simple insulin thing so complicated, lol! Also, where did you buy the sprayer? How did you ask? Thanks again for sharing

Edited by cougar, 07 March 2016 - 01:54 AM.

[Remington]

Hey Remington! Thanks for sharing, very helpful. But I guess I will still have to answer the question of how many I need to buy. What's the unit, for example, 1000 ml or 100 vial? If I say I want Novolin R, are they going to ask me more detailed question, for example Novolin R alpha, or Novolin R beta etc? What if they don't have Novolin R? I was amazed at how they've made a simple insulin thing so complicated, lol! Also, where did you buy the sprayer? How did you ask? Thanks again for sharing

Heres the Novolin info but this is Novolin N you want the R I'm sure. It's $25 and also I didn't purchase the nasal spray through the pharmacy it should be down the Isle with the sinus allergy sprays. I just bought a cheap one and poured out the solution, cleaned out the bottle and poured the Novolin in the bottle.

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