29 votes
Posted 05 December 2017 - 02:17 PM
Hi Lostfalco,
Could you describe/illustrate the 9 regions you're putting the 96-LED on?
"5. 850nm, 96 LED, 9 individual "regions" on sides, top, and back of head AND 850nm, 48 LED, 5 individual spots on forehead along hairline (roughly)."
Posted 19 December 2017 - 12:04 PM
Posted 22 December 2017 - 08:52 AM
Dear Lostfalco:
Longtime lurker and fan, first time commenter. I greatly admire your nootropic daring, and I'm glad you're busy with a great new job.
I picked up LLLT and INI from you, thanks!
You said a few months ago, "Right now, I am testing every element of Dr. Bredesen's protocol simultaneously (with a few of my own additions)." I'm an intellectual, but not educated in life sciences, 68 years old and APOE 4/4. Thus, I'm very interested in your explorations and discoveries with the protocol. Bredesen mentions a few substances in his book that are quite obscure and that remain unmentioned at apoe4.info. Also, by the way, there is a an analytic elaboration of the Bredesen protocol at Anti-Aging Firewalls that might interest you. http://www.anti-agin...art-1-the-plan/
Happy to hear your results someday.
Best,
Bladedmind
Posted 03 January 2018 - 02:36 AM
Hi Lostfalco,
Could you describe/illustrate the 9 regions you're putting the 96-LED on?
"5. 850nm, 96 LED, 9 individual "regions" on sides, top, and back of head AND 850nm, 48 LED, 5 individual spots on forehead along hairline (roughly)."
Hey Judd, the main idea is to try to laser your entire brain. Don't get too caught up on the exact regions. I just found that I could get the front, back, and sides with about 9 non-overlapping placements of the 96 LED.
Posted 03 January 2018 - 03:11 AM
Hi,have anyone looked at this study from S. Moskvin at the State Scientific Center of Laser Medicine in Moscow:The title is "Only lasers can be used for low level laser therapy", and the conclusion is"Thus, NON-laser light sources (lamps with or without filters, LEDs with or without a polarizer, etc.) cannot be used in low level laser therapy because of their minimal efficiency."I have a vetrolaser, but for convenience I have been using LEDs instead. This makes me think it might be a good idea to go back to using the laser.
Hey Olorin, this is a very strange, emotional article (ie. not very scientific). I wouldn't let it sway your decision of which device to use. This was a hotly debated topic 20 years ago but the majority of LLLT practitioners now think that LEDs are just effective as lasers for one reason: LEDs work in experiments.
Here are a few recent examples. =)
https://www.ncbi.nlm...pubmed/28464523
Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice.
We aimed to investigate the effects of low-level light emitting diode therapy (LED-T) on the long-term functional outcomes after cerebral ischemia, and the optimal timing of LED-T initiation for achieving suitable functional recovery. Focal cerebral ischemia was induced in mice via photothrombosis. These mice were assigned to a sham-operated (control), ischemic (vehicle), or LED-T group [initiation immediately (acute), 4 days (subacute) or 10 days (delayed) after ischemia, followed by once-daily treatment for 7 days]. Behavioral outcomes were assessed 21 and 28 days post-ischemia, and histopathological analysis was performed 28 days post-ischemia. The acute and subacute LED-T groups showed a significant improvement in motor function up to 28 days post-ischemia, although no brain atrophy recovery was noted. We observed proliferating cells (BrdU+ ) in the ischemic brain, and significant increases in BrdU+ /GFAP+ , BrdU+ /DCX+ , BrdU+ /NeuN+ , and CD31+ cells in the subacute LED-T group. However, the BrdU+ /Iba-1+ cell count was reduced in the subacute LED-T group. Furthermore, the brain-derived neurotrophic factor (BDNF) was significantly upregulated in the subacute LED-T group. We concluded that LED-T administered during the subacute stage had a positive impact on the long-term functional outcome, probably via neuron and astrocyte proliferation, blood vessel reconstruction, and increased BDNF expression. Picture: The rotarod test for motor coordination showed that acute and subacute LED-T improves long-term functional recovery after cerebral ischemia.
https://www.ncbi.nlm...pubmed/28347821
Photostimulation with low-level light emitting diode therapy (LED-T) modulates neurological and psychological functions. The purpose of this study was to evaluate the effects of LED-T pretreatment on the mouse brain after ischemia/reperfusion and to investigate the underlying mechanisms. Ischemia/reperfusion brain injury was induced by middle cerebral artery occlusion. The mice received LED-T twice a day for 2 days prior to cerebral ischemia. After reperfusion, the LED-T group showed significantly smaller infarct and edema volumes, fewer behavioral deficits compared to injured mice that did not receive LED-T and significantly higher cerebral blood flow compared to the vehicle group. We observed lower levels of endothelial nitric oxide synthase (eNOS) phosphorylation in the injured mouse brains, but significantly higher eNOS phosphorylation in LED-T-pretreated mice. The enhanced phospho-eNOS was inhibited by LY294002, indicating that the effects of LED-T on the ischemic brain could be attributed to the upregulation of eNOS phosphorylation through the phosphoinositide 3-kinase (PI3K)/Akt pathway. Moreover, no reductions in infarct or edema volume were observed in LED-T-pretreated eNOS-deficient (eNOS-/-) mice. Collectively, we found that pretreatment with LED-T reduced the amount of ischemia-induced brain damage. Importantly, we revealed that these effects were mediated by the stimulation of eNOS phosphorylation via the PI3K/Akt pathway.
https://www.ncbi.nlm...pubmed/28001756
We review the general topic of traumatic brain injury (TBI) and our research utilizing transcranial photobiomodulation (tPBM) to improve cognition in chronic TBI using red/near-infrared (NIR) light-emitting diodes (LEDs) to deliver light to the head. tPBM improves mitochondrial function increasing oxygen consumption, production of adenosine triphosphate (ATP), and improving cellular energy stores. Nitric oxide is released from the cells increasing regional blood flow in the brain. Review of published studies: In our previously published study, 11 chronic TBI patients with closed-head TBI caused by different accidents (motor vehicle accident, sports-related, improvised explosive device blast injury) and exhibiting long-lasting cognitive dysfunction received 18 outpatient treatments (Monday, Wednesday, Friday for 6 weeks) starting at 10 months to 8 years post-TBI. LED therapy is nonthermal, painless, and noninvasive. An LED-based device classified as nonsignificant risk (FDA cleared) was used. Each LED cluster head (5.35 cm diameter, 500 mW, 22.2 mW/cm2) was applied for 9 min 45 sec (13 J/cm2) using 11 locations on the scalp: midline from front-to-back hairline and bilaterally on frontal, parietal, and temporal areas. Testing was performed before and after transcranial LED (tLED; at 1 week, 1 month, and at 2 months after the 18th treatment) and showed significant improvements in executive function and verbal memory. There were also fewer post-traumatic stress disorder (PTSD) symptoms reported. Ongoing studies: Ongoing, current studies involve TBI patients who have been treated with tLED using either 26 J/cm2 per LED location on the head or treated with intranasal only (iLED) using red (633 nm) and NIR (810 nm) diodes placed into the nostrils. The NIR iLED is hypothesized to deliver photons to the hippocampus, and the red 633 nm iLED is believed to increase melatonin. Results have been similar to the previously published tLED study. Actigraphy sleep data showed increased time asleep (on average one additional hour per night) after the 18th tLED or iLED treatment. LED treatments may be performed in the home. Sham-controlled studies with veterans who have cognitive dysfunction from Gulf War Illness, blast TBI, and TBI/PTSD are currently ongoing.
Edited by lostfalco, 03 January 2018 - 03:13 AM.
Posted 03 January 2018 - 03:17 AM
Dear Lostfalco:
Longtime lurker and fan, first time commenter. I greatly admire your nootropic daring, and I'm glad you're busy with a great new job.
I picked up LLLT and INI from you, thanks!
You said a few months ago, "Right now, I am testing every element of Dr. Bredesen's protocol simultaneously (with a few of my own additions)." I'm an intellectual, but not educated in life sciences, 68 years old and APOE 4/4. Thus, I'm very interested in your explorations and discoveries with the protocol. Bredesen mentions a few substances in his book that are quite obscure and that remain unmentioned at apoe4.info. Also, by the way, there is a an analytic elaboration of the Bredesen protocol at Anti-Aging Firewalls that might interest you. http://www.anti-agin...art-1-the-plan/
Happy to hear your results someday.
Best,
Bladedmind
Hey Bladedmind, thanks so much! I'm glad you've gained something from some of my experiments.
I'm still testing everything from Bredesen's protocol. I'll be sharing one of these days (hopefully soon).
Thanks for the link. I like Vince's site so I'll definitely have to check it out.
Posted 04 January 2018 - 01:35 PM
Hi Lostfalco,
I was wondering if LLLT on mice possibly has a better effect because of how small their skulls are?
It seems the light can penetrate at least one inch into the human brain. Wouldn't the light be able to penetrate the entire brain of mice?
Apologies if this question was asked before!
Thank you either way.
Posted 06 January 2018 - 01:30 AM
I am contemplating to buy a joovv device to use on my head because it scares me to use a device intended for a completely different purpose. Do you guys think this is wasted money?
The cheapest one I saw was $495!
I'd test out a $20 device first before spending that much.
Hi Lostfalco,
I was wondering if LLLT on mice possibly has a better effect because of how small their skulls are?
It seems the light can penetrate at least one inch into the human brain. Wouldn't the light be able to penetrate the entire brain of mice?
Apologies if this question was asked before!
Thank you either way.
Their skulls are definitely thinner than ours which is why dosing is adjusted for humans.
Here are affordable devices to try out and how to calculate dosing for humans. http://www.lostfalco...therapy-dosing/
Edited by lostfalco, 06 January 2018 - 01:34 AM.
Posted 09 January 2018 - 05:50 PM
Enjoy!
https://www.ncbi.nlm...pubmed/29307593
Sleep deprivation (SD) causes oxidative stress in the hippocampus and subsequent memory impairment. In this study, the effect of near-infrared (NIR) photobiomodulation (PBM) on learning and memory impairment induced by acute SD was investigated. The mice were subjected to an acute SD protocol for 72 hr. Simultaneously, NIR PBM using a laser at 810 nm was delivered (once a day for 3 days) transcranially to the head to affect the entire brain of mice. The Barnes maze and the What-Where-Which task were used to assess spatial and episodic-like memories. The hippocampal levels of antioxidant enzymes and oxidative stress biomarkers were evaluated. The results showed that NIR PBM prevented cognitive impairment induced by SD. Moreover, NIR PBM therapy enhanced the antioxidant status and increased mitochondrial activity in the hippocampus of SD mice. Our findings revealed that hippocampus-related mitochondrial damage and extensive oxidative stress contribute to the occurrence of memory impairment. In contrast, NIR PBM reduced hippocampal oxidative damage, supporting the ability of 810 nm laser light to improve the antioxidant defense system and maintain mitochondrial survival. This confirms that non-invasive transcranial NIR PBM therapy ameliorates hippocampal dysfunction, which is reflected in enhanced memory function.
Posted 13 January 2018 - 09:47 AM
Hi Lostfalco,
I was wondering if LLLT on mice possibly has a better effect because of how small their skulls are?
It seems the light can penetrate at least one inch into the human brain. Wouldn't the light be able to penetrate the entire brain of mice?
Apologies if this question was asked before!
Thank you either way.Their skulls are definitely thinner than ours which is why dosing is adjusted for humans.
Here are affordable devices to try out and how to calculate dosing for humans. http://www.lostfalco...therapy-dosing/
Thank you for the reply and the link. I read it but I think I am still misunderstanding or I worded my previous post badly-
Since mice brains are so small, and the light can fully penetrate throughout (reaching every part and the hippocampus completely), how would dosing adjustments for humans compensate?
Considering the light penetrates one inch into the human brain, I assume that we have a limitation in effect compared to the full light penetration with mice?
P.S:
I have been using the 10,000 lux sunbright product each morning for eye exposure and it has made me feel more alert, energetic, and most important of all... it boosts my mood!
Edit: Not sure if chitosan has been discussed in this thread, but I am going to start researching it tomorrow. Seems it increases intranasal administration through the olfactory nerve tissue and is effective with NGF. I wonder if it can be added into insulin without compromising the solution.
https://www.ncbi.nlm...pubmed/19156912
https://www.ncbi.nlm...pubmed/21879386
Edited by highlightfocus, 13 January 2018 - 09:51 AM.
Posted 06 March 2018 - 07:08 PM
Hello,
I believe I have reached the edge of what I can achieve with nootropics and other chemicals, now I am looking into LLLT.
Has anyone tried the Vielight Neuro Alpha or Gamma? It's very costly, but it seems to solve the problem of not knowing where to aim and for how long.
If I am mainly looking for curing mental fatigue and executive function, what should I buy and where should I am.
Sorry I know there are hundreds of pages of info, but I just don't know how to dig through it all without getting lost. Thanks!
Posted 24 June 2018 - 04:59 PM
Has anyone used LLLT for a long time and then stopped, and observed any negative effects? Typically when the body is given anything externally it stops creating it internally and when the external source stops it can be difficult to get the body's natural production restarted again. I would imagine (guess) that it would be wise to cycle LLLT but I am also looking for anecdotes if anyone has some experience with this.
Posted 24 June 2018 - 06:23 PM
Has anyone used LLLT for a long time and then stopped, and observed any negative effects? Typically when the body is given anything externally it stops creating it internally and when the external source stops it can be difficult to get the body's natural production restarted again. I would imagine (guess) that it would be wise to cycle LLLT but I am also looking for anecdotes if anyone has some experience with this.
Posted 08 August 2018 - 03:21 PM
This is so stinking cool!
Who would have thought that mitochondria would be so important? lol
https://www.scienced...80807095140.htm
"The compounds in question -- AP39, AP123 and RT01 -- have been designed by the Exeter team to selectively deliver minute quantities of the gas hydrogen sulfide to the mitochondria in cells and help the old or damaged cells to generate the 'energy' needed for survival and to reduce senescence.
"Our compounds provide mitochondria in cells with an alternative fuel to help them function properly," said Professor Matt Whiteman, also from the University of Exeter.
"Many disease states can essentially be viewed as accelerated ageing, and keeping mitochondria healthy helps either prevent or, in many cases using animal models, reverse this.
"Our current study shows that splicing factors play a key role in determining how our compounds work."
Here's the study if anyone wants to check it out: https://www.ncbi.nlm...pubmed/30026406
Mitochondria-targeted hydrogen sulfide attenuates endothelial senescence by selective induction of splicing factors HNRNPD and SRSF2.
Cellular senescence is a key driver of ageing, influenced by age-related changes to the regulation of alternative splicing. Hydrogen sulfide (H2S) has similarly been described to influence senescence, but the pathways by which it accomplishes this are unclear.We assessed the effects of the slow release H2S donor Na-GYY4137 (100 µg/ml), and three novel mitochondria-targeted H2S donors AP39, AP123 and RT01 (10 ng/ml) on splicing factor expression, cell proliferation, apoptosis, DNA replication, DNA damage, telomere length and senescence-related secretory complex (SASP) expression in senescent primary human endothelial cells.All H2S donors produced up to a 50% drop in senescent cell load assessed at the biochemical and molecular level. Some changes were noted in the composition of senescence-related secretory complex (SASP); IL8 levels increased by 24% but proliferation was not re-established in the culture as a whole. Telomere length, apoptotic index and the extent of DNA damage were unaffected. Differential effects on splicing factor expression were observed depending on the intracellular targeting of the H2S donors. Na-GYY4137 produced a general 1.9 - 3.2-fold upregulation of splicing factor expression, whereas the mitochondria-targeted donors produced a specific 2.5 and 3.1-fold upregulation of SRSF2 and HNRNPD splicing factors only. Knockdown of SRSF2 or HNRNPD genes in treated cells rendered the cells non-responsive to H2S, and increased levels of senescence by up to 25% in untreated cells.Our data suggest that SRSF2 and HNRNPD may be implicated in endothelial cell senescence, and can be targeted by exogenous H2S. These molecules may have potential as moderators of splicing factor expression and senescence phenotypes.
Edited by lostfalco, 08 August 2018 - 03:28 PM.
Posted 08 August 2018 - 04:58 PM
Has anyone used LLLT for a long time and then stopped, and observed any negative effects? Typically when the body is given anything externally it stops creating it internally and when the external source stops it can be difficult to get the body's natural production restarted again. I would imagine (guess) that it would be wise to cycle LLLT but I am also looking for anecdotes if anyone has some experience with this.
Do you cycle sunlight?
Not sure if you are referring to Laser or LED therapy Basicallyyes. If LED, I've done some months, and stopped (not related to the therapy). No negatives, rather I've been feeling like I need to start again, and just have done so.
MettMett, keep in mind that sunlight is a wide continuous spectrum, i.e., all the light frequencies and far beyond at both ends of the spectrum, with many frequencies harmful. LLLT uses one or a few very specific frequencies that interact with specific the biological processes to elicit a desired result.
Posted 09 August 2018 - 01:58 AM
lostfalco, do u still get the cheaper version of ibudilast or you gave up on it? because i cannot find it, maybe ill get any of yours if you still have
Hey hazy, I get the expensive stuff from mimaki pharmacy and take low doses to make it more affordable. https://www.mimaki-f...item_branch=001
Posted 09 August 2018 - 11:18 AM
This is so stinking cool!
Who would have thought that mitochondria would be so important? lol
https://www.scienced...80807095140.htm
"The compounds in question -- AP39, AP123 and RT01 -- have been designed by the Exeter team to selectively deliver minute quantities of the gas hydrogen sulfide to the mitochondria in cells and help the old or damaged cells to generate the 'energy' needed for survival and to reduce senescence.
"Our compounds provide mitochondria in cells with an alternative fuel to help them function properly," said Professor Matt Whiteman, also from the University of Exeter.
"Many disease states can essentially be viewed as accelerated ageing, and keeping mitochondria healthy helps either prevent or, in many cases using animal models, reverse this.
"Our current study shows that splicing factors play a key role in determining how our compounds work."
Here's the study if anyone wants to check it out: https://www.ncbi.nlm...pubmed/30026406
Aging (Albany NY). 2018 Jul 19;10(7):1666-1681. doi: 10.18632/aging.101500.Mitochondria-targeted hydrogen sulfide attenuates endothelial senescence by selective induction of splicing factors HNRNPD and SRSF2.
AbstractCellular senescence is a key driver of ageing, influenced by age-related changes to the regulation of alternative splicing. Hydrogen sulfide (H2S) has similarly been described to influence senescence, but the pathways by which it accomplishes this are unclear.We assessed the effects of the slow release H2S donor Na-GYY4137 (100 µg/ml), and three novel mitochondria-targeted H2S donors AP39, AP123 and RT01 (10 ng/ml) on splicing factor expression, cell proliferation, apoptosis, DNA replication, DNA damage, telomere length and senescence-related secretory complex (SASP) expression in senescent primary human endothelial cells.All H2S donors produced up to a 50% drop in senescent cell load assessed at the biochemical and molecular level. Some changes were noted in the composition of senescence-related secretory complex (SASP); IL8 levels increased by 24% but proliferation was not re-established in the culture as a whole. Telomere length, apoptotic index and the extent of DNA damage were unaffected. Differential effects on splicing factor expression were observed depending on the intracellular targeting of the H2S donors. Na-GYY4137 produced a general 1.9 - 3.2-fold upregulation of splicing factor expression, whereas the mitochondria-targeted donors produced a specific 2.5 and 3.1-fold upregulation of SRSF2 and HNRNPD splicing factors only. Knockdown of SRSF2 or HNRNPD genes in treated cells rendered the cells non-responsive to H2S, and increased levels of senescence by up to 25% in untreated cells.Our data suggest that SRSF2 and HNRNPD may be implicated in endothelial cell senescence, and can be targeted by exogenous H2S. These molecules may have potential as moderators of splicing factor expression and senescence phenotypes.
I have very good experience with MitoQ which is also a mito-targeted compound. Essentially the only dietary supplement that ever lived up to the hype imo.
Waiting for SkQ1 with some annoyance.
Posted 09 August 2018 - 11:52 AM
MettMett, keep in mind that sunlight is a wide continuous spectrum, i.e., all the light frequencies and far beyond at both ends of the spectrum, with many frequencies harmful. LLLT uses one or a few very specific frequencies that interact with specific the biological processes to elicit a desired result.
Posted 09 August 2018 - 11:55 AM
This is so stinking cool!
Who would have thought that mitochondria would be so important? lol
https://www.scienced...80807095140.htm
"The compounds in question -- AP39, AP123 and RT01 -- have been designed by the Exeter team to selectively deliver minute quantities of the gas hydrogen sulfide to the mitochondria in cells and help the old or damaged cells to generate the 'energy' needed for survival and to reduce senescence.
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Posted 09 August 2018 - 02:04 PM
Not sure what blue lights you are referring to, but no I don't. The LLLT I use is 660nm, i.e., deep red.
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