• Log in with Facebook Log in with Twitter Log In with Google      Sign In    
  • Create Account
  LongeCity
              Advocacy & Research for Unlimited Lifespans

Photo
* * * * * 29 votes

Lostfalco's Extensive Nootropic Experiments [Curated]

nootropic

  • Please log in to reply
4029 replies to this topic

#1501 chung_pao

  • Guest
  • 352 posts
  • 92
  • Location:Sweden.

Posted 19 November 2013 - 09:29 PM

http://www.ncbi.nlm....pubmed/24102298
More potential mitochondrial biogenesis upregulators
1. bezafibrate
2. resveratrol
3. AICAR

Target: PGC-1α http://en.wikipedia.org/wiki/PPARGC1A

"PGC-1α is a transcriptional coactivator that regulates the genes involved in energy metabolism. PGC-1α is a regulator of mitochondrial biogenesis and function.[3]"



Have you tried Resveratrol as a PDE-4 inhibitor, to be used standalone with fasting or Forskolin?
There are studies touting it as a pde-4 inhibitor, but I can't find anyone who has tested it as a potential replacement of Artichoke, or as an addition to the CILTEP-stack.

#1502 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 19 November 2013 - 10:22 PM

http://www.ncbi.nlm....pubmed/24102298
More potential mitochondrial biogenesis upregulators
1. bezafibrate
2. resveratrol
3. AICAR

Target: PGC-1α http://en.wikipedia.org/wiki/PPARGC1A

"PGC-1α is a transcriptional coactivator that regulates the genes involved in energy metabolism. PGC-1α is a regulator of mitochondrial biogenesis and function.[3]"



Have you tried Resveratrol as a PDE-4 inhibitor, to be used standalone with fasting or Forskolin?
There are studies touting it as a pde-4 inhibitor, but I can't find anyone who has tested it as a potential replacement of Artichoke, or as an addition to the CILTEP-stack.

Hey, what's up Chung? It's been a while man. Hope you're doing well.

I haven't tried Resveratrol for that purpose...only Artichoke. How's CILTEP been holding up for you? You've been on it for a quite a while now if I remember correctly.

sponsored ad

  • Advert
Click HERE to rent this advertising spot for BRAIN HEALTH to support LongeCity (this will replace the google ad above).

#1503 EncyclopediaBrown

  • Guest
  • 44 posts
  • 19
  • Location:US

Posted 20 November 2013 - 11:15 PM

Effect of the Mildronate in the cell

http://youtu.be/uk-eiAUgO9w

Edited by EncyclopediaBrown, 20 November 2013 - 11:16 PM.


#1504 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 21 November 2013 - 03:14 PM

Effect of the Mildronate in the cell

http://youtu.be/uk-eiAUgO9w

Very interesting. Thanks EBrown!

#1505 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 21 November 2013 - 03:37 PM

So, I think I've mentioned this supplement before but aarfai introduced me to it a few months ago http://www.longecity...330#entry599276 and I find myself simultaneously intrigued and skeptical (since it's creator claims to be Nikola Tesla reincarnated...for real!). It claims to consist of hydride ions (H-....hydrogen with two electrons) attached to silica. This extra electron is easily donated thereby acting as a powerful antioxidant. Has anyone tried this? What do you guys think? http://www.amazon.co...s/dp/B000GFPD7O

Edited by lostfalco, 21 November 2013 - 03:37 PM.

  • like x 2

#1506 Joe Cohen

  • Guest
  • 156 posts
  • -10
  • Location:USA

Posted 21 November 2013 - 06:09 PM

-How are people's experiences with shilajit. I took 8X the suggested dosage and I'm still not sure if I feel anything, and I'm super sensitive. I will try it again and if still not will take it to 12X.

-I'm experimenting with an earthing mat. I **MAY** feel something, but the problem is there's no way to up the dosage, like I usually do if I'm not sure if something is working. Could be placebo.

-I'd like to recommend Clay to people. Powerful sh*t. Take 4g if you want to feel it on an empty stomach. Not necessarily completely good. I have to figure out what it's doing exactly.

-One of the best noots is SUNLIGHT, believe or not. I've been experimenting with it over 4 months and it is powerful stuff.

-LF, I'm going to try to put drinking water in the sun and see if I notice anything. I'm interested in trying your halogen experiment.

Edited by Joe Cohen, 21 November 2013 - 06:09 PM.


#1507 gwern

  • Guest
  • 77 posts
  • 27
  • Location:USA

Posted 21 November 2013 - 06:18 PM

-One of the best noots is SUNLIGHT, believe or not. I've been experimenting with it over 4 months and it is powerful stuff.


FWIW, I analyzed years of my own data and found no influence: http://gwern.net/Weather Possibly the vitamin D or melatonin screen off any sunlight influences.
  • like x 1

#1508 Metagene

  • Guest
  • 674 posts
  • 78
  • Location:Florida
  • NO

Posted 21 November 2013 - 08:07 PM

-How are people's experiences with shilajit. I took 8X the suggested dosage and I'm still not sure if I feel anything, and I'm super sensitive. I will try it again and if still not will take it to 12X.


How long have you taken it? Beneficial effects might be more pronounced over a period of weeks rather than something you would notice after consuming.

I didn't feel much outside of endurance level increase when on it.




Sent from my iPhone using Tapatalk

#1509 Godof Smallthings

  • Guest
  • 710 posts
  • 136
  • Location:Thailand

Posted 22 November 2013 - 07:35 AM

I've taken shilajit together with CoQ 10, and, occasionally, PQQ for some time now. Can't say I notice much difference. Jarrow's shilajit is the one I am taking at present. Before that, I finished a bottle of Natural Factor's shilajit. Nothing definitive.

Treating my mother, who has hip osteoarthritis, with the 48 LED (30 sec per sore spot, essentially the hip joint and then down the leg along one of the muscles which is the most sore), shilajit, CoQ10 and vit C since a few days. She also drinks a glass of collagen in the afternoon.

Before she started this regime, she could only walk for 10 minutes continuously and then be in pain, now she is up to 23 min, and can move around the rest of the day without too much pain. Placebo or not, I do not know, but we'll keep doing it as long as it seems to be working.
  • like x 1

#1510 Nattzor

  • Guest
  • 549 posts
  • 103
  • Location:Sweden

Posted 23 November 2013 - 02:11 PM

No idea if it has been posted before, but gonna read it tonight. http://www.ncbi.nlm....les/PMC2790317/ - "Biphasic Dose Response in Low Level Light Therapy"

#1511 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 23 November 2013 - 04:19 PM

“Researchers at Mid Sweden University compared the effects of caffeine and blue light on the brain and found them both to have a positive effect. Interestingly, people exposed to blue light performed better on tests of brain function that included a distraction.”

"The study builds on research that suggests blue light can improve cognitive abilities such as memory, alertness and reaction time. Although harmless to tissue, blue light is strong enough to trigger biological effects in the body, and it’s being used increasingly for a range of medical treatments."

http://www.dailymail.co.uk/health/article-2511979/Could-blue-lights-replace-daily-cup-coffee-Scientists-claim-effective-keeping-alert-caffeine.html#ixzz2lS0GaBmK

This effect is most likely caused by photosensitive ganglion cells. http://en.wikipedia.org/wiki/Photosensitive_ganglion_cell

"Most work suggests that the peak spectral sensitivity of the receptor is between 460 and 484 nm. Lockley et al. in 2003 showed that 460 nm (violet) wavelengths of light suppress melatonin twice as much as 555 nm (green) light, the peak sensitivity of the photopic visual system. In work by Zaidi, Lockley and co-authors using a rodless, coneless human, it was found that what consciously led to light perception was a very intense 481 nm stimulus; this means that the receptor in visual terms enables some rudimentary vision maximally for blue light.[4]"

I've been using this device first thing in the morning for over 6 months now. It makes me very alert. http://www.amazon.co...s=suntouch plus

If you are having trouble falling asleep then make sure and block blue light from reaching your photosensitive ganglion cells at least one hour before you want to fall asleep. If you block blue light, then endogenous melatonin will be increased. Try these. Works like a charm. =) http://www.amazon.co... safety glasses

BigPapaChakra showed me some recent articles suggesting that certain wavelengths of red light might enhance alertness as well...without suppressing melatonin production. Here's one: http://www.scienceda...30422100801.htm

Edited by lostfalco, 23 November 2013 - 04:30 PM.


#1512 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 23 November 2013 - 04:53 PM

-How are people's experiences with shilajit. I took 8X the suggested dosage and I'm still not sure if I feel anything, and I'm super sensitive. I will try it again and if still not will take it to 12X.

Hey, what's up Joe? I have not taken shilajit by itself but I have taken CoQ10 with it and without it. I've subjectively noticed 'more energy' and 'more sustained energy' with the shilajit as compared to CoQ10 alone. As always, take that anecdote for what it's worth. =)

-I'm experimenting with an earthing mat. I **MAY** feel something, but the problem is there's no way to up the dosage, like I usually do if I'm not sure if something is working. Could be placebo.

Very cool. I'll be interested to hear your results over time. I'm still a little skeptical about earthing but not completely closed to the idea. Which mat did you decide to try out?

-LF, I'm going to try to put drinking water in the sun and see if I notice anything. I'm interested in trying your halogen experiment.

Nice. I'm very interested to see if you replicate my results. I honestly wasn't expecting any change at all but I'm cool with 99% blood oxygenation and a resting heart rate that's leveled off around 53bpm. Tbh, I can't say that I 'feel' the change...but I'll take it anyway. I use this pulse oximeter to measure my results. http://www.walgreens...6089451-product

Edited by lostfalco, 23 November 2013 - 04:54 PM.


#1513 Joe Cohen

  • Guest
  • 156 posts
  • -10
  • Location:USA

Posted 23 November 2013 - 05:03 PM

-How are people's experiences with shilajit. I took 8X the suggested dosage and I'm still not sure if I feel anything, and I'm super sensitive. I will try it again and if still not will take it to 12X.

Hey, what's up Joe? I have not taken shilajit by itself but I have taken CoQ10 with it and without it. I've subjectively noticed 'more energy' and 'more sustained energy' with the shilajit as compared to CoQ10 alone. As always, take that anecdote for what it's worth. =)

-I'm experimenting with an earthing mat. I **MAY** feel something, but the problem is there's no way to up the dosage, like I usually do if I'm not sure if something is working. Could be placebo.

Very cool. I'll be interested to hear your results over time. I'm still a little skeptical about earthing but not completely closed to the idea. Which mat did you decide to try out?

-LF, I'm going to try to put drinking water in the sun and see if I notice anything. I'm interested in trying your halogen experiment.

Nice. I'm very interested to see if you replicate my results. I honestly wasn't expecting any change at all but I'm cool with 99% blood oxygenation and a resting heart rate that's leveled off around 53bpm. Tbh, I can't say that I 'feel' the change...but I'll take it anyway. I use this pulse oximeter to measure my results. http://www.walgreens...6089451-product


Ya, I'm really skeptical of the whole grounding thing as well, but grounding does have an effect on the body, so it's not implausible.

My blood oxygenation is usually 99% and resting heart rate is 60 or under generally, so not sure what it will do for me on that front.

#1514 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 23 November 2013 - 05:32 PM

-One of the best noots is SUNLIGHT, believe or not. I've been experimenting with it over 4 months and it is powerful stuff.


FWIW, I analyzed years of my own data and found no influence: http://gwern.net/Weather Possibly the vitamin D or melatonin screen off any sunlight influences.

How's it going gwern? Have you looked into photosensitive ganglion cells, blue light therapy, red light therapy, and the effects of temperature on the rate of chemical reactions in the human body? I would love to see you apply your meticulous measuring and analytic abilities to such phenomenona...at some point. I know you're super busy. =)

Red and Blue Light
http://www.ncbi.nlm....pubmed/23535242

Effect of Temperature on Brain Function
http://www.ncbi.nlm....pubmed/23274506
http://www.ncbi.nlm....pubmed/23300680
http://www.ncbi.nlm..../pubmed/8646277

Representative Quote:
"By interacting with neural mechanisms regulating metabolic activity and heat exchange between the brain and the rest of the body, neuroactive drugs either increase or decrease brain temperatures both within (35-39 °C) and exceeding the range of physiological fluctuations. These temperature effects differ drastically depending upon the environmental conditions and activity state during drug administration. This state-dependence is especially important for drugs of abuse that are usually taken by humans during psycho-physiological activation and in environments that prevent proper heat dissipation from the brain. Under these conditions, amphetamine-like stimulants induce pathological brain hyperthermia (>40 °C) associated with leakage of the blood-brain barrier and structural abnormalities of brain cells."

Edited by lostfalco, 23 November 2013 - 05:49 PM.


#1515 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 23 November 2013 - 05:40 PM

Ya, I'm really skeptical of the whole grounding thing as well, but grounding does have an effect on the body, so it's not implausible.

My blood oxygenation is usually 99% and resting heart rate is 60 or under generally, so not sure what it will do for me on that front.

Mine was 98% before and heart rate was usually around 60 to 62ish...so it might do something. Or...maybe I changed something else without realizing it. =)

Are you still experimenting with lactate? What's your current recommendation there? Still sourdough bread?

btw...have you tried Hydrogen Boost? I bought some two days ago and it really seems like a powerful antioxidant. I'd love to hear your thoughts on it. It's only about $13-$14 on amazon. http://www.amazon.co...8&condition=new

Edited by lostfalco, 23 November 2013 - 05:46 PM.


#1516 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 23 November 2013 - 06:04 PM

Pulsed Electromagnetic Field Products (for those interested) =)
http://drpawluk.com/store/

This guy is a salesman...so be careful. Still...some interesting ideas for possible enhancement.
http://www.pemfbook....ecommendations/

#1517 stephen_b

  • Guest
  • 1,744 posts
  • 240

Posted 23 November 2013 - 06:10 PM

I've been using this device first thing in the morning for over 6 months now. It makes me very alert. http://www.amazon.co...s=suntouch plus


Hi lostfalco. Were you getting your good results from near IR therapy before you started with the blue light in the mornings?

#1518 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 23 November 2013 - 06:15 PM

I've been using this device first thing in the morning for over 6 months now. It makes me very alert. http://www.amazon.co...s=suntouch plus


Hi lostfalco. Were you getting your good results from near IR therapy before you started with the blue light in the mornings?

Hey Stepen. Yeah. I used LLLT for at least 3+ months (probably more like 5 or 6) before adding blue light first thing in the morning.

#1519 OpaqueMind

  • Guest
  • 471 posts
  • 144
  • Location:UK
  • NO

Posted 23 November 2013 - 06:22 PM

I've been using an earthing mat under my bedsheets every night and it's been really effective at improving my sleep quality, as well as dream recall and intensity. It also deepens my meditation, perhaps because it reduces cortisol - http://www.ncbi.nlm....ubmed/15650465. I'll be honest, I'm not a biochemist, I'm just an experimenter and earthing had such universally good reviews I thought I'd invest in a mat. It's been amazing for just these two things.

How I know it's not a placebo - I've tried a ridiculous number of interventions of various kinds to improve my fragmented and suboptimal sleep over the last few years, and most of them have done very little, definitely nothing on the scale of this. Also, paradoxically, the first few nights I used the mat I had very disturbed sleep and intense dreams - apparently this is normal as the body adjusts to the difference. After that, my sleep got slowly more efficacious and now I wake up feeling rested! That is a major shift from my previous efforts. It has easily been the most effective sleep remedy I've used, along with blue-light-blocking glasses.

From the little I know of antioxidants, they donate electrons to neutralise free radicals, so if this truly works by pumping electrons into the body directly then it is essentially a lifetime supply of practically unlimited antioxidants! I suppose science is nothing if it does not transcend the banality of 'common sense', as this very thread attends.

#1520 BigPapaChakra

  • Guest
  • 199 posts
  • 32
  • Location:Illinois, USA

Posted 23 November 2013 - 07:02 PM

I definitely wouldn't dismiss earthing, but there are some very important aspects to it that are overlooked. I personally wouldn't use an earthing sheet without a proper understanding of how the wiring in my home, in addition to the outlets, are in terms of quality. In Clint Ober's Earthing book, he goes into brief but significant detail about how many homes are improperly wired, have problems with outlets, and a whole host of other things, and that when using an earthing sheet they become an antennae for dirty electricity in that wiring. I think earthing via contact with the ground or water (sea/ocean/lake/etc.) or creating your own earthing sheets with grounding rods attached to the earth outside, finally followed by getting earthing pads/sheets only after testing your home, are all the best options.

I also think that earthing has a lot to do with geo/biomagnetism, as opposed to simply the free electrons. So, earthing will be great, if you go and do yoga barefoot in a park or run at a beach, I'd guarantee you'd feel an increase in your mood and recovery, and if you do it chronically your health will probably improve. Yet, unfortunately, that wouldn't be enough as our earth's beneficial magnetic fields have been greatly diminished, the Schumann Resonance is harder to sense, AND there is a whole host of other detrimental frequencies picked up by our Superchaismatic Nucleus. This is why other things such as PEMF, magnetic sleep pads, jewelry, etc. are necessary.

Recently, Paul Becker sent me an "only once used" EarthPulse as a gift to both cure my younger brother of his diseases, and aid in my mental/psych./emotional recovery (from HPPD, family problems, etc.). It's pretty crazy how this thing works. My body definitely needs a period of adaptation. When receiving it, you get a bunch of web links and pdf files on recommended and experimental use of the product. I have to drastically weaken the strength of the device to get a good nights rest - I put it at the end of my bed, close to where my feet lie, while also detaching the screw in the magnet as to weaken the force and make it less concentrated. When I have it closer, roughly where the center of my back is, I get put into this very weird state, EarthPulse refers to this as agitation. I've almost experienced OBEs and Astral Projection before, getting into the state where you lose awareness of your body, feel vibrations, get odd sensory images, yet are completely mentally awake; well, when I have the EP too close (in Sleep-Mode 3) I get put into that state. I'm literally dreaming and awake at the same time - I start experiencing dream like sensations and lose sense of my body though I 'know' I'm still 'awake' and can hear everything around me outside/in my room/etc. I'll stay in this state ALL night if I don't diminish the power. Fortunately, after awhile, I should be able to move the magnet closer and closer after a few weeks.

I'm going to quantify my experience as best as possible and blog about it and share my experience here at this thread; they recommend testing your resting breath hold as a proxy for oxygen utilization, retention, and OXPHOS (oxidative phosphorylation) capabilities. I'm also researching Broda Barnes, so, I'm also recording my body temperature and pulse, along with body comp and posture (as I have a really bad back, even at my young age (disc bulges, bad shoulder blades, and a screwed up right side of my body)).

I believe that if you emailed Paul Becker, depending on your situation and your personal discussion with him, you may be able to receive a used one, work out payment plans, or perhaps negotiate a lower price. Just don't quote me on that. Paul is an extremely genuine and kind individual, although he can start to 'rant' about conspiratorial events that have taken place, lol.
  • like x 2

#1521 OpaqueMind

  • Guest
  • 471 posts
  • 144
  • Location:UK
  • NO

Posted 23 November 2013 - 07:19 PM

Also, I've been using NeurOptimal for the last few months, and experienced awesome results. So far I've done about 30 sessions, and evidently I have quite a way to go because I still hear glitches in the audio track while using it, which supposedly correspond to impending aberrations in brain function... they're pretty black box about their mechanisms.

Nonetheless, the experience has been profound - what I've experienced thus far, and what continues to be on the increase are the following factors; memory, relaxation ability, connectivity between thoughts (gestalt factor), mind-body connection, length and intensity of focus, depth of meditation (this is huge, I've been meditation for two years and I'd average the depth increase at an extra 200%, so 3x deeper), ability to empathise, unstressability (I was pretty awesome at that anyway haha), better creativity (playing guitar and singing in particular) which seems to come from a greater ability to access the flow state, richer and more detailed sensorium, more endurance (?), faster reactions, greater motivation, proneness to frission, and just the general awesomeness of life has been ramped up. There's probably more I'm missing out, but you get the picture.

I haven't tried any other forms of EEG neurofeedback yet, so I cannot compare them in their effectiveness, and I wouldn't endorse one over the other, but if you have a practitioner who uses neurofeedback in your area definitely check it out for at least a dozen sessions. Also, be on the look out for second-hand versions, given the original supplier is so damn expensive. I'm so blown away by it that I've started hooking a friend up to it - I feel it's almost my duty to help him revolutionise his existence. You can't keep all this amazing shit to yourself, not when you see that you could help better someones path through this life to such a degree. Hence my review here - hopefully some words will inspire some of you to look deeper into this... there are plenty of testimonials online.

From what little I know of the mechanism and my interpretation of said mechanism, this is like super-charged meditation. To illustrate this we can borrow an ancient metaphor from buddhism; the mind is like a body of water, and the bright moon, representing reality, is reflected on this water. Thoughts are disturbances that perturb this body and obscure the reflection of reality. The turbulence of the discursive mind ripples in waves across the surface and makes it hard to see the true reflection. When the energy of aberrant thought is relinquished, the body of water naturally comes to rest in stillness - then the reflection is seen in its fullness. Meditation is the practice of letting go of these disturbances we attach to, by the act of simple observing them with our whole being. This is all well and good, but it takes a long long time to reconfigure these patterns by simply being still, and turbulence bubbles up from the unrestful depths of the psyche regardless. Using NeurOptimal is akin to producing counter waves which inversely mirror these disturbances and so induce a kind of phase cancellation, stilling the water(mind) through direct and opposite action. Each disturbance is countered before its ripple can propagate into widespread chaos, and over time the tendency of the water to fluctuate in emotional and distractive response diminishes.

By the way, I am very intrigued about your experiences with PEMF Papa, do keep us updated! That looks seriously awesome. May have to treat myself for christmas :)

Edited by OpaqueMind, 23 November 2013 - 07:43 PM.

  • like x 3

#1522 abelard lindsay

  • Guest
  • 873 posts
  • 227
  • Location:Mare Serenitatis Circumlunar Corporate Republic

Posted 23 November 2013 - 07:43 PM

I recently had a friend asking me about laser therapy. What do you guys think of this intranasal laser product? Yes, you stick it up your nose.

http://www.mediclights.com/

Here's the product matrix:
http://www.vielight....evice-category/

Looks like it supports 633 655 and 810 nm

Anyway, interesting little device. Will also make for a great Wirehead Haloween costume. :)

Edited by abelard lindsay, 23 November 2013 - 07:46 PM.


#1523 Nattzor

  • Guest
  • 549 posts
  • 103
  • Location:Sweden

Posted 23 November 2013 - 07:57 PM

I recently had a friend asking me about laser therapy. What do you guys think of this intranasal laser product? Yes, you stick it up your nose.

http://www.mediclights.com/

Here's the product matrix:
http://www.vielight....evice-category/

Looks like it supports 633 655 and 810 nm

Anyway, interesting little device. Will also make for a great Wirehead Haloween costume. :)

http://en.wikipedia.org/wiki/Blood_irradiation_therapy

I personally think it'll provide sub-optimal results compared to doing it IV, the effects that it's suppose to produce I have no idea about. The claimed effects seem to have very little to no research behind it, but wort experimenting with. It seems to be recommended to use UVB though, so I guess a normal LED would not work. If I build a helmet (goddammit, need to restart my experiment so it'll be done) I'll probably add 2 white diodes for the ears (valkee-style) and maybe some red for the nose.

#1524 Nattzor

  • Guest
  • 549 posts
  • 103
  • Location:Sweden

Posted 23 November 2013 - 08:43 PM



5. POSSIBLE EXPLANATIONS FOR BIPHASIC DOSE RESPONSE IN LLLT

The repeated observations that have been made of the biphasic dose response phenomenon in LLLT require some explanation. The natural assumption that is frequently made is, that if a small dose of red or near-infrared light produces a significant therapeutic effect, then a larger dose should produce an even more beneficial effect. This natural assumption is frequently not the case. We here propose three possible explanations for the existence of the biphasic dose response based upon mechanistic considerations outlined in section 2.

5.1. Excessive ROS

As discussed in 2.5 the light mediated generation of reactive oxygen species has been observed in many in vitro studies and has been proposed to account for the cellular changes observed after LLLT via activation of redox sensitive transcription factors (Chen et al. 2009a). The evidence of ROS mediated activation of NF-κB in MEF cells presented in 4.1 provides additional support for this hypothesis (Chen et al. 2009a). It is well-accepted that ROS can have both beneficial and harmful effects (Huang and Zheng 2006). Hydrogen peroxide is often used to kill cells in vitro (Imlay 2008). Other ROS such as singlet oxygen (Klotz et al. 2003) and hydroxyl radicals (Pryor et al. 2006) are thought to be harmful even at low concentrations. The concept of biphasic dose response in fact is well established in the field of oxidative stress (Day and Suzuki 2005). If the generation of ROS can be shown to be dose dependent on the delivered energy fluence this may provide an explanation for the stimulation and inhibition observed with low and high light fluences.

5.2. Excessive NO

The other mechanistic hypothesis that is put forward to explain the cellular effects of LLLT relates to the photolysis of nitrosylated proteins that releases free NO (see section 2.6). Again the literature has many papers that discuss the so-called two-faced or “Janus” molecule NO (Anggard 1994; Lane and Gross 1999). NO can be either protective or harmful depending on the dose and particularly on the cell or tissue type where it is generated (Calabrese 2001a).

5.3. Activation of a cytotoxic pathway

The third hypothesis to explain the biphasic dose response of LLLT is the idea that the protective and stimulatory effects of light occur at low doses, but there is an additional pathway that leads to damaging effects of light that only occurs at high doses, and effectively overwhelms the beneficial effects of low doses of light. Work from South China Normal University provides some support for this hypothesis. Low doses of LLLT were found to phosphorylate hepatocyte growth factor receptor (c-Met), and initiate signaling via cyclic AMP and Jun kinase and Src (Gao and Xing 2009). By contrast, high dose LLLT was found to induce apoptosis via a mitochondrial caspase-3 pathway and cytochrome c release was attributed to opening of the mitochondrial permeability transition pore caused by high-level intracellular reactive oxygen species (ROS) generation (Wu et al. 2009). A secondary signaling pathway through Bax activation was observed (Wu et al. 2009).

- http://www.ncbi.nlm....les/PMC2790317/

Fairly good paper on explaining on why too much is bad and too little can be bad.
  • like x 1

#1525 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 25 November 2013 - 07:27 PM

Cardiolipin, a critical determinant of mitochondrial carrier protein assembly and function

http://www.ncbi.nlm....les/PMC2757529/

"CL is almost exclusively associated with membranes charged with the task of generating an electrochemical gradient that is used to produce ATP. Such membranes include the mitochondrial inner membrane and the bacterial plasma membrane. The mitochondrial inner membrane has an unusually high ~3-4:1 protein:lipid ratio. In contrast, the protein:lipid ratio of the mitochondrial outer membrane is ~1–1.6:1 [51-53]. While the contribution of CL to the establishment of this remarkably high concentration of proteins in the inner membrane has yet to be demonstrated, it is noteworthy that CL has the ability to interact with a number of different proteins, including all of the major players involved in oxidative phosphorylation [4, 6, 54-65]. Much recent attention has focused on the importance of CL for the structural organization of the respiratory complexes in higher order structures of functional importance [5, 7, 8]. A modern appreciation of the importance of CL in the structure and function of another critical component of the oxidative phosphorylation machinery, the ADP/ATP carrier (AAC), has ostensibly been forgotten. A comparison of the assembly status of oligomers of the ATP synthase, the respiratory supercomplexes, and AAC in wild type (+CL) and Δcrd1 (- CL) yeast clearly demonstrates the relative importance of CL for AAC (Fig. 1). Therefore, in this review, I will briefly discuss the importance of CL in the formation of the respiratory supercomplexes. I will then turn my attention to the critical role of CL for the normal structure and function of AAC, the charter member of the expanding mitochondrial carrier family."

Cardiolipin wiki http://en.wikipedia....iki/Cardiolipin

Edited by lostfalco, 25 November 2013 - 08:07 PM.


#1526 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 26 November 2013 - 12:22 AM

Very interesting interview with an electrical expert on grounding (esp. in the U.S.) and electrical pollution. I'm still forming my views on these topics and thought I would share. =)

Stetzer recommends standing on a sheet of aluminum foil for 2 minutes multiple times throughout the day in order to discharge the electrical charge that builds up on your body (sounds like a cheap enough experiment). He strongly advises against using the earthing mats (in the U.S.) that you plug into the wall or into the ground because 70% of the electrons sent to your home return to the power station through the ground (according to him, of course).

Edited by lostfalco, 26 November 2013 - 01:16 AM.


#1527 lostfalco

  • Topic Starter
  • Guest
  • 1,686 posts
  • 414
  • Location:the present

Posted 26 November 2013 - 02:47 AM

Ummm...alright, this water stuff is getting crazy! You guys have to (if you have time) check out this conversation between Gerald Pollack and physicist Dan Nelson. They talk about levitating frogs with magnetic fields, turning water into gold, and using lasers to change the hydrogen bonding angle of water. I'm not saying that I believe any of this...but holy crap it's fascinating. Let me know what you guys think. The convo starts around 29:40 and takes a few minutes to really get going.


#1528 Neal Cullum

  • Guest
  • 48 posts
  • 2
  • Location:united kingdom

Posted 26 November 2013 - 05:51 PM

Hi everyone. I've bought myself a 96 850NM LED lamp in the hopes that i'll find some benefit from it. When i plugged it in i was expecting bright light but what i've got is a very dim light coming out the bulbs. Is this supposed to be normal? Here is the link to what i bought off ebay :- http://www.ebay.co.u...984.m1497.l2649

Hi everyone. I've bought myself a 96 850NM LED lamp in the hopes that i'll find some benefit from it. When i plugged it in i was expecting bright light but what i've got is a very dim light coming out the bulbs. Is this supposed to be normal? Here is the link to what i bought off ebay :- http://www.ebay.co.u...984.m1497.l2649


Wrong link, this is the right one:- http://www.ebay.co.u...US_Surveillance

#1529 p3x888

  • Guest
  • 133 posts
  • 16
  • Location:USA
  • NO

Posted 26 November 2013 - 08:04 PM

Hi everyone. I've bought myself a 96 850NM LED lamp in the hopes that i'll find some benefit from it. When i plugged it in i was expecting bright light but what i've got is a very dim light coming out the bulbs. Is this supposed to be normal? Here is the link to what i bought off ebay :- http://www.ebay.co.u...984.m1497.l2649

Wrong link, this is the right one:- http://www.ebay.co.u...US_Surveillance


The LEDs are near infrared - this means you will see only a slight red light emit from the device. If it was true infrared, you wouldn't see anything. This means the human eye simply can't see those wavelengths but rest ashured, the light is coming out.

You should be able to feel them warming up.

Edited by p3x888, 26 November 2013 - 08:05 PM.


sponsored ad

  • Advert
Click HERE to rent this advertising spot for BRAIN HEALTH to support LongeCity (this will replace the google ad above).

#1530 Neal Cullum

  • Guest
  • 48 posts
  • 2
  • Location:united kingdom

Posted 26 November 2013 - 08:43 PM

Hi everyone. I've bought myself a 96 850NM LED lamp in the hopes that i'll find some benefit from it. When i plugged it in i was expecting bright light but what i've got is a very dim light coming out the bulbs. Is this supposed to be normal? Here is the link to what i bought off ebay :- http://www.ebay.co.u...984.m1497.l2649

Wrong link, this is the right one:- http://www.ebay.co.u...US_Surveillance


The LEDs are near infrared - this means you will see only a slight red light emit from the device. If it was true infrared, you wouldn't see anything. This means the human eye simply can't see those wavelengths but rest ashured, the light is coming out.

You should be able to feel them warming up.


Thanks for your reply. I'm a total noob to this LLLT so is this device i've got suitable? I know this LLLT is in the experimental stages for most but i just don't want to be using the wrong type of LED lamp and think that LLLT doesn't work for me.





Also tagged with one or more of these keywords: nootropic

139 user(s) are reading this topic

0 members, 139 guests, 0 anonymous users

Topic Led By