238 replies to this topic

[Hebbeh]

I'll start looking for quotes. Might take a while given the backlog of other compounds of interest. But, this one takes priority ATM.

This sounds interesting. I'd be interested in giving it a go.

[Reformed-Redan]

Cool, we'll see the results from JPC16 and if good we'll go for ISRIB.

[phil8462643]

I am always ready to throw in on anything interesting! and this one seems to be, so i am in too!

[solarfingers]

Certainly have my attention...

[MasterHerb]

I would be interested in a group buy.

[Nattzor]

For OP (or well, the dude who bought it): Are you planning on selling some or just keeping it?

[Reformed-Redan]

For OP (or well, the dude who bought it): Are you planning on selling some or just keeping it?

Well, yeah. He bought some after I asked him to supply C16 for another member. So, yeah he wants to make a profit. Lol @ the Limitless pic in OP. If C16 is good, which we will find out soon, then we should go with ISRIB. It works by the same or similar mechanism to enhance memory encoding.

[Reformed-Redan]

I don't know, but they do the same thing, I think.
PKR inhibition which leads to inhibition of eif2 alpha phosphorylation which leads to the prevention of inhibition of translation. And translation is good for memory formation.

Could you please quote the study that states the above because this study just came out today.

Memory-Boosting Chemical Identified in Mice: Cell Biologists Find Molecule Targets a Key Biological Pathway June 14, 2013 — Memory improved in mice injected with a small, drug-like molecule discovered by UCSF San Francisco researchers studying how cells respond to biological stress.

The same biochemical pathway the molecule acts on might one day be targeted in humans to improve memory, according to the senior author of the study, Peter Walter, PhD, UCSF professor of biochemistry and biophysics and a Howard Hughes Investigator. The discovery of the molecule and the results of the subsequent memory tests in mice were published in eLife, an online scientific open-access journal, on May 28, 2013. In one memory test included in the study, normal mice were able to relocate a submerged platform about three times faster after receiving injections of the potent chemical than mice that received sham injections. The mice that received the chemical also better remembered cues associated with unpleasant stimuli -- the sort of fear conditioning that could help a mouse avoid being preyed upon. Notably, the findings suggest that despite what would seem to be the importance of having the best biochemical mechanisms to maximize the power of memory, evolution does not seem to have provided them, Walter said. "It appears that the process of evolution has not optimized memory consolidation; otherwise I don't think we could have improved upon it the way we did in our study with normal, healthy mice," Walter said. The memory-boosting chemical was singled out from among 100,000 chemicals screened at the Small Molecule Discovery Center at UCSF for their potential to perturb a protective biochemical pathway within cells that is activated when cells are unable to keep up with the need to fold proteins into their working forms. However, UCSF postdoctoral fellow Carmela Sidrauski, PhD, discovered that the chemical acts within the cell beyond the biochemical pathway that activates this unfolded protein response, to more broadly impact what's known as the integrated stress response. In this response, several biochemical pathways converge on a single molecular lynchpin, a protein called eIF2 alpha. Scientists have known that in organisms ranging in complexity from yeast to humans different kinds of cellular stress -- a backlog of unfolded proteins, DNA-damaging UV light, a shortage of the amino acid building blocks needed to make protein, viral infection, iron deficiency -- trigger different enzymes to act downstream to switch off eIF2 alpha. "Among other things, the inactivation of eIF2 alpha is a brake on memory consolidation," Walter said, perhaps an evolutionary consequence of a cell or organism becoming better able to adapt in other ways. Turning off eIF2 alpha dials down production of most proteins, some of which may be needed for memory formation, Walter said. But eIF2 alpha inactivation also ramps up production of a few key proteins that help cells cope with stress. Study co-author Nahum Sonenberg, PhD, of McGill University previously linked memory and eIF2 alpha in genetic studies of mice, and his lab group also conducted the memory tests for the current study. The chemical identified by the UCSF researchers is called ISRIB, which stands for integrated stress response inhibitor. ISRIB counters the effects of eIF2 alpha inactivation inside cells, the researchers found. "ISRIB shows good pharmacokinetic properties [how a drug is absorbed, distributed and eliminated], readily crosses the blood-brain barrier, and exhibits no overt toxicity in mice, which makes it very useful for studies in mice," Walter said. These properties also indicate that ISRIB might serve as a good starting point for human drug development, according to Walter. Walter said he is looking for scientists to collaborate with in new studies of cognition and memory in mouse models of neurodegenerative diseases and aging, using ISRIB or related molecules. In addition, chemicals such as ISRIB could play a role in fighting cancers, which take advantage of stress responses to fuel their own growth, Walter said. Walter already is exploring ways to manipulate the unfolded protein response to inhibit tumor growth, based on his earlier discoveries. At a more basic level, Walter said, he and other scientists can now use ISRIB to learn more about the role of the unfolded protein response and the integrated stress response in disease and normal physiology. Additional UCSF study authors are Diego Acosta-Alvear, PhD, Punitha Vedantham, PhD, Brian Hearn, PhD, Ciara Gallagher, PhD, Kenny Ang, PhD, Chris Wilson, PhD, Voytek Okreglak, PhD, Byron Hann, MD, PhD, Michelle Arkin, PhD, and Adam Renslo, PhD. Other authors are Han Li, PhD, and Avi Ashkenazi, PhD, from Genentech; and, Karim Nader, PhD, Karine Gamache, and Arkady Khoutorsky, PhD, from McGill University. The study was funded by the Howard Hughes Medical Institute.

From here. The results seem similar to that of C16 although this seems like a more stable chemical. Anyone have the full study?

[solarfingers]

Yadayada, You were going to look into having a lab create this for a group buy. What is the progress of this and did you source ISRIB as well?

This all seems a bit academic if there are no easily accessible sources for either of these two chemicals. Wouldn't any reliable chemical synthesis company be concerned about licensing issues if making it for the group? I'm sure that using the product in "research" would be considered viable for educational purposes. I guess my only concern is where OP getting the C16 and how pure is it?

Thanks...

[Reformed-Redan]

Yadayada, You were going to look into having a lab create this for a group buy. What is the progress of this and did you source ISRIB as well?

This all seems a bit academic if there are no easily accessible sources for either of these two chemicals. Wouldn't any reliable chemical synthesis company be concerned about licensing issues if making it for the group? I'm sure that using the product in "research" would be considered viable for educational purposes. I guess my only concern is where OP getting the C16 and how pure is it?

Thanks...

Well, at the moment I am quite busy with trying to get the PRL-8-53 group buy moving again and waiting to see results from ScienceGuy on IDRA-21. I also almost finished the BPAP group buy. But, if I had found this compound earlier (before PRL, IDRA-21), I would have pushed to get it first as it seems the most promising of the rest of the compounds for memory enhancement. However, I will wait till things clear up. The OP got his batch from Sigma-Aldrich. Also, as a reminder everything on these forums is strictly for research only.

[solarfingers]

Wow , you are busy! I need to look into these other chems you are referencing. Sigma-Aldrich is a good source. My chickens are eagerly waiting...

[YOLF]

I'm wondering if my cat would do tricks again, or even learn new ones.

[Reformed-Redan]

I'm wondering if my cat would do tricks again, or even learn new ones.

That cat is awesome.

[YOLF]

Thanks Have you seen his C60 thread?
http://www.longecity...ther-cat-trial/

[Reformed-Redan]

Thanks Have you seen his C60 thread?
http://www.longecity...ther-cat-trial/

I just had to get a better look at that picture:

Meow!~ :D

Post some more photos of your cat, lol. Hahahah.

[solarfingers]

Oh, that is a nice cat.

[lostfalco]

God you have a nice pussy! (you were all thinking it)

"That cat was the best fuck I ever had." -Steve Martin

Zsa Zsa with a cat in her lap: Johnny would you like to pet my pussy?
Johnny: Sure, if you move that cat out of the way.

[YOLF]

Thanks Have you seen his C60 thread?
http://www.longecity...ther-cat-trial/

I just had to get a better look at that picture:

Meow!~ :D

Post some more photos of your cat, lol. Hahahah.

Careful, he'll yeow your head off! He's part Snowshoe. I love yeowing back though, it's like we're actually talking about something. I'll post more pics later this afternoon and maybe some videos too. That pic did turn out great too, unfortunately the vet apparently had to shave him on the neck to take some blood, so a patch of his fur is missing. He's really a cool cat though, if you've ever seen that 80s/90s commercial for "My Buddy" the stuffed toy, the song fits him perfectly.

[Reformed-Redan]

Really great news. ISRIB can be synthesized relatively easily by our trusted European Custom Synthesis company.

10 participants at $99 each will get 2.5g of ISRIB. Anyone able and willing, just post here.

Effective doses in lab rats was 2.5mg ot 5mg per kilogram.

[JPC16]

I am in.

[Metagene]

Yes please yadayada! My wallet is ready.

[sunshinefrost]

I would hop in as well.

[Nattzor]

Really great news. ISRIB can be synthesized relatively easily by our trusted European Custom Synthesis company.

10 participants at $99 each will get 2.5g of ISRIB. Anyone able and willing, just post here.

Effective doses in lab rats was 2.5mg ot 5mg per kilogram.

If you haven't changed the dosage, just remember to do so. You usually divide it by 10-15 (or use the actual formula).

But I thought they said it was active in the mcg range earlier in the thread?

[Googoltarian]

Anyone able and willing, just post here.

NO! There is enough trash/ off-topic posting here!

Don't take me the wrong way, ISRIB is very interesting, but please start another thread.

[Reformed-Redan]

Anyone able and willing, just post here.

NO! There is enough trash/ off-topic posting here!

Don't take me the wrong way, ISRIB is very interesting, but please start another thread.

Sorry, will start a new thread.

[megatron]

I'm a 100% in!

[Reformed-Redan]

Please post here:
http://www.longecity...srib-group-buy/

[MasterHerb]

Any updates on c16?

[JPC16]

No, I am right in the middle of getting the compound transported to me. But we had some minor setbacks (DHL woudn't transport it).
But I'll will very probably still get the compound before the ISRIB comes around though.

[Reformed-Redan]

No, I am right in the middle of getting the compound transported to me. But we had some minor setbacks (DHL woudn't transport it).
But I'll will very probably still get the compound before the ISRIB comes around though.

Awesome. Hope it works out!