14 replies to this topic

[lostfalco]

Please share your thoughts on how to enhance exercise results using bioenergetics. =) http://en.wikipedia....i/Bioenergetics

[BigPapaChakra]

Re-post from other thread: http://www.youtube.c...u7CUXkDfs#t=836 This is a laboratory controlled case study by the creator of the Cool Fat Burner Vest - there was an increase in RMR by 301% (that was sustained for a long time); anecdotally in his personal experience he increased insulin sensitivity and learned that he could enter ketosis faster (probably due to either depletion of glycogen from prolonged shivering, or, drops in blood glucose in addition to increased catecholamines).

I'm thinking of a winter experiment - deep CT with special emphasis on CT during training and during periodic overfeeds to gain mass and strength. I have not yet fully applied Dr. Kruse CT protocol, mainly due to being incapable of taking ice baths at the moment along with toxins in my current home that would lead to awful detox reactions, thus I'm waiting until I move. Once I do, having access to a fitness center at the apartment, cold weather from Winter, properly operating AC - I'm giving it a go. I was thinking that a mix of the Cool Fat Burner + the soon to be released Gut Buster while doing high intensity resistance training or HIIT would lead to unfathomable gains. Vasper uses this with much success, and anecdotally, if I take cold showers prior to lifting weights I'm stronger and fatigue less quickly, I could only imagine the results of actually remaining cold throughout the duration of a workout. Oh yeah, another anecdote - Dr. Dough McGuff of Body By Science has said on Dr. Kruse CT blogs that he and the other trainers and researchers notice drastic increases in exercise performance when working out in a 61* Fahrenheit room.

Here is Vasper: http://vasper.com/ (intra-workout CT + compression + grounding = 2hr workout in 20 minutes)

[BigPapaChakra]

I feel as though Brown Adipose Tissue and Beige Adipose Tissue (there's a difference) formation could be a strong addition to TULIP. From the Mitochondrial Panel Discussion I posted on the main thread, I learned that the three largest sources of mitochondria are the brain, heart, and skeletal-muscle tissue. Well, Brown Adipose Tissue is more embryologically similar to muscle than fat tissue. It's brown due to its vast source of mitochondria. Substances that activate AMPK are touted as being exercise in a pill, well, maybe we actually can recreate some of the benefits of exercise via other means. Maybe creating more BAT and Beige Adipose Tissue (BAT and BAT, lol) gives us more space for mitochondria, allowing us to do even more with TULIP.

http://edrv.endojour...t/34/3/413.full

"Brown adipocytes contain well-developed mitochondria filling most of the cytoplasm. The high density of mitochondria is comparable to that of cardiomyocytes. In contrast, the mitochondrial content of white adipocytes is low. BAT is also endowed with a rich blood and nerve supply and a dense niche of perivascular mesenchymal cells, which are a nursery of preadipocytes. The abundance of mitochondria containing respiratory chain cytochrome enzymes with iron as a cofactor, as well as the vasculature within BAT, gives rise to a darker red (brown) color, compared with the paler hue exhibited by WAT."

"Brown and beige adipocytes are also enriched for the transcriptional coactivator PGC1-α, which directs the expression of key regulatory molecules responsible for mitochondrial biogenesis in response to external stimuli, such as cold exposure (50). Once differentiated, these different types of adipocytes (and the depots enriched in these cell types) can be identified by their unique gene expression signatures (68)."

"PGC-1α not only is enriched in classic BAT depots but also determines the extent of BAT enrichment within WAT. Although PGC-1α expression is low in WAT, it can be stimulated by cold exposure or β-adrenergic agents (50). Ectopic overexpression of PGC-1α in an adipocyte cell line induced the expression of UCP1 and increased mitochondrial content, leading to the emergence of multilobulated lipid droplets that are indistinguishable from brown adipocytes (50). Mice lacking PGC-1α specifically in WAT and BAT exhibit a blunted induction of UCP1 mRNA expression to cold exposure (51) or PPAR-γ agonist (52). These results provide evidence for PGC-1α as a key factor regulating brown adipogenesis."

PQQ has effects on PGC-1a.

Now this just opened a whole different rabbit hole - damnit!!!

[lostfalco]

Great posts Papa! Those are some fascinating and unique ideas. I've been thinking about this lately. 1. laser fat to access stored fat energy, 2. lift/cardio using this fat energy and 3. laser to enhance muscle recovery. There are a lot of pubmed studies on lasering fat and lasering muscles. Let me know your thoughts on these ideas. We could possibly combine this with a vibration plate and/or a cold thermogenesis vest for absurdly quick body recomp.

"LLLT stimulates the mitochondria in adipocytes to increase adenosine triphosphate (ATP) synthesis with subsequent up regulation of cyclic adenosine monophosphate (cAMP). The increase of cAMP stimulates cytoplasmic lipase which converts triglycerides into fatty acids and glycerol, which can pass through pores formed in the cell membrane [4]." http://www.ncbi.nlm....les/PMC3680639/

[lostfalco]

Review article of LLLT and muscle recovery.

Conclusion: Exposing skeletal muscle to single-diode and multidiode laser or multidiode LED therapy was shown to positively affect physical performance by delaying the onset of fatigue, reducing the fatigue response, improving postexercise recovery, and protecting cells from exercise-induced damage.

http://www.ncbi.nlm....pubmed/23672326

[BigPapaChakra]

• Wilson JM, Joy JM, Lowery RP, Roberts MD, Lockwood CM, Manninen AH, Fuller JC, De Souza EO, Baier SM, Wilson SMC, Rathmacher JA. Effects of oral adenosine-5[prime]-triphosphate supplementation on athletic performance, skeletal muscle hypertrophy and recovery in resistance-trained men. Nutrition & Metabolism 2013, 10:57.

Oral ATP supplementation 30 minutes before exercise has beneficial effects on exercise performance, recovery, hypertrophy, etc.

http://www.bengreenf...genesis-how-to/ Dr. Kruse and Ben Greenfield on Cold Thermogenesis for exercise and sports performance

http://www.bengreenf...ur-life-easier/ Ben Greenfield with Vasper engineer.

[lostfalco]

Recent review article on LLLT and fat loss. (I apologize if I've posted this article before =))

"Results: The studies as of today suggest that LLLT has a potential to be used in fat and cellulite reduction as well as in improvement of blood lipid profile without any significant side effects."

http://www.ncbi.nlm....pubmed/23749426

Lasers Surg Med. 2013 Aug;45(6):349-57. doi: 10.1002/lsm.22153. Epub 2013 Jun 7.

Low-level laser therapy for fat layer reduction: a comprehensive review.
Avci P, Nyame TT, Gupta GK, Sadasivam M, Hamblin MR.

Source
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

Abstract
BACKGROUND AND OBJECTIVE:
Low-level laser (light) therapy (LLLT) is a noninvasive, nonthermal approach to disorders requiring reduction of pain and inflammation and stimulation of healing and tissue regeneration. Within the last decade, LLLT started being investigated as an adjuvant to liposuction, for noninvasive body contouring, reduction of cellulite, and improvement of blood lipid profile. LLLT may also aid autologous fat transfer procedures by enhancing the viability of adipocytes. However the underlying mechanism of actions for such effects still seems to be unclear. It is important, therefore, to understand the potential efficacy and proposed mechanism of actions of this new procedure for fat reduction.

MATERIALS AND METHODS:
A review of the literature associated with applications of LLLT related to fat layer reduction was performed to evaluate the findings from pre-clinical and clinical studies with respect to the mechanism of action, efficacy, and safety.

RESULTS:
The studies as of today suggest that LLLT has a potential to be used in fat and cellulite reduction as well as in improvement of blood lipid profile without any significant side effects. One of the main proposed mechanism of actions is based upon production of transient pores in adipocytes, allowing lipids to leak out. Another is through activation of the complement cascade which could cause induction of adipocyte apoptosis and subsequent release of lipids.

CONCLUSION:
Although the present studies have demonstrated safety and efficacy of LLLT in fat layer reduction, studies demonstrating the efficacy of LLLT as a stand-alone procedure are still inadequate. Moreover, further studies are necessary to identify the mechanism of action.

Edited by lostfalco, 06 October 2013 - 02:02 PM.

[Judd Crane]

I'm curious if LLLT could be used against "puffy nipples". Or make it worse by stimulating prolactin?

Edited by Judd Crane, 06 October 2013 - 02:11 PM.

[lostfalco]

I feel as though Brown Adipose Tissue and Beige Adipose Tissue (there's a difference) formation could be a strong addition to TULIP. From the Mitochondrial Panel Discussion I posted on the main thread, I learned that the three largest sources of mitochondria are the brain, heart, and skeletal-muscle tissue. Well, Brown Adipose Tissue is more embryologically similar to muscle than fat tissue. It's brown due to its vast source of mitochondria. Substances that activate AMPK are touted as being exercise in a pill, well, maybe we actually can recreate some of the benefits of exercise via other means. Maybe creating more BAT and Beige Adipose Tissue (BAT and BAT, lol) gives us more space for mitochondria, allowing us to do even more with TULIP.

http://edrv.endojour...t/34/3/413.full

"Brown adipocytes contain well-developed mitochondria filling most of the cytoplasm. The high density of mitochondria is comparable to that of cardiomyocytes. In contrast, the mitochondrial content of white adipocytes is low. BAT is also endowed with a rich blood and nerve supply and a dense niche of perivascular mesenchymal cells, which are a nursery of preadipocytes. The abundance of mitochondria containing respiratory chain cytochrome enzymes with iron as a cofactor, as well as the vasculature within BAT, gives rise to a darker red (brown) color, compared with the paler hue exhibited by WAT."

"Brown and beige adipocytes are also enriched for the transcriptional coactivator PGC1-α, which directs the expression of key regulatory molecules responsible for mitochondrial biogenesis in response to external stimuli, such as cold exposure (50). Once differentiated, these different types of adipocytes (and the depots enriched in these cell types) can be identified by their unique gene expression signatures (68)."

"PGC-1α not only is enriched in classic BAT depots but also determines the extent of BAT enrichment within WAT. Although PGC-1α expression is low in WAT, it can be stimulated by cold exposure or β-adrenergic agents (50). Ectopic overexpression of PGC-1α in an adipocyte cell line induced the expression of UCP1 and increased mitochondrial content, leading to the emergence of multilobulated lipid droplets that are indistinguishable from brown adipocytes (50). Mice lacking PGC-1α specifically in WAT and BAT exhibit a blunted induction of UCP1 mRNA expression to cold exposure (51) or PPAR-γ agonist (52). These results provide evidence for PGC-1α as a key factor regulating brown adipogenesis."

PQQ has effects on PGC-1a.

Now this just opened a whole different rabbit hole - damnit!!!

Papa, have you tried lasering your brown fat yet?

I'm still working through my thoughts on cold exposure and fat loss. What are your thoughts on this article? http://download.cell...termediate=true

Edited by lostfalco, 06 October 2013 - 02:31 PM.

[lostfalco]

Muscle regeneration in elderly rats using 830nm light.

Quick Summary:
1. Increased muscle regeneration.
2. Increased capillary blood count.
3. Increase in MyoD and VEGF gene expression.

http://www.ncbi.nlm....les/PMC3671618/

Photonics Lasers Med. 2012 Oct 1;1(4):287-297.

Low intensity laser therapy accelerates muscle regeneration in aged rats.
Vatansever F, Rodrigues NC, Assis LL, Peviani SS, Durigan JL, Moreira FM, Hamblin MR, Parizotto NA.

Source
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA; and Department of Dermatology, Harvard Medical School, Boston, MA, USA.

Abstract
BACKGROUND:
Elderly people suffer from skeletal muscle disorders that undermine their daily activity and quality of life; some of these problems can be listed as but not limited to: sarcopenia, changes in central and peripheral nervous system, blood hypoperfusion, regenerative changes contributing to atrophy, and muscle weakness. Determination, proliferation and differentiation of satellite cells in the regenerative process are regulated by specific transcription factors, known as myogenic regulatory factors (MRFs). In the elderly, the activation of MRFs is inefficient which hampers the regenerative process. Recent studies found that low intensity laser therapy (LILT) has a stimulatory effect in the muscle regeneration process. However, the effects of this therapy when associated with aging are still unknown.

OBJECTIVE:
This study aimed to evaluate the effects of LILT (λ=830 nm) on the tibialis anterior (TA) muscle of aged rats.

SUBJECTS AND METHODS:
The total of 56 male Wistar rats formed two population sets: old and young, with 28 animals in each set. Each of these sets were randomly divided into four groups of young rats (3 months of age) with n=7 per group and four groups of aged rats (10 months of age) with n=7 per group. These groups were submitted to cryoinjury + laser irradiation, cryoinjury only, laser irradiation only and the control group (no cryoinjury/no laser irradiation). The laser treatment was performed for 5 consecutive days. The first laser application was done 24 h after the injury (on day 2) and on the seventh day, the TA muscle was dissected and removed under anesthesia. After this the animals were euthanized. Histological analyses with toluidine blue as well as hematoxylin-eosin staining (for counting the blood capillaries) were performed for the lesion areas. In addition, MyoD and VEGF mRNA was assessed by quantitative polymerase chain reaction.

RESULTS:
The results showed significant elevation (p<0.05) in MyoD and VEGF genes expression levels. Moreover, capillary blood count was more prominent in elderly rats in laser irradiated groups when compared to young animals.

CONCLUSION:
In conclusion, LILT increased the maturation of satellite cells into myoblasts and myotubes, enhancing the regenerative process of aged rats irradiated with laser.

Edited by lostfalco, 06 October 2013 - 03:52 PM.

[lostfalco]

Conclusions: These results suggest that LLLT could be an effective therapeutic approach in increasing muscle performance during a resistance exercise protocol.

http://www.ncbi.nlm....pubmed/24102167

Photomed Laser Surg. 2013 Oct;31(10):492-498.
Effect of Low-Level Laser Therapy (808 nm) in Skeletal Muscle After Resistance Exercise Training in Rats.

Patrocinio T, Sardim AC, Assis L, Fernandes KR, Rodrigues N, Renno AC.

Source
1 Department of Physiotherapy, Federal University of São Carlos , São Carlos, São Paulo, Brazil .

Abstract
Abstract Objective: The aim of this study was to evaluate the effects of 808 nm laser applied after a resistance training protocol, on biochemical markers and the morphology of skeletal muscle in rats. Background data: Strenuous physical activity results in fatigue and decreased muscle strength, impaired motor control, and muscle pain. Many biochemical and biophysical interventions have been studied in an attempt to accelerate the recovery process of muscle fatigue. Among these, low-level laser therapy (LLLT) has been demonstrated to be effective in increasing skeletal muscle performance in in vivo studies and in clinical trials. However, little is known about the effects of LLLT on muscle performance after resistance training. Methods: Thirty Wistar rats were randomly divided into three groups: control group (CG), trained group (TG), and trained and laser-irradiated group (TGL). The resistance training program was performed three times per week for 5 weeks, and consisted of a climbing exercise, with weights attached to the tail of the animal. Furthermore, laser irradiation was performed in the middle region of tibialis anterior (TA) muscle of both legs, after the exercise protocol. Results: Analysis demonstrated that TGL (training group with laser) demonstrated significantly reduced resting lactate level and decreased muscle glycogen depletion than the animals that were exercised only, and significantly increased the cross-section area of TA muscle fibers compared with thoseo in the other groups. Conclusions: These results suggest that LLLT could be an effective therapeutic approach in increasing muscle performance during a resistance exercise protocol.

Edited by lostfalco, 11 October 2013 - 12:10 AM.

[lostfalco]

"The champions' mitochondria: is it genetically determined? A review on mitochondrial DNA and elite athletic performance"

http://physiolgenomi.../43/13/789.long

"One of the numerous adaptations to regular aerobic (endurance) exercise, which distinguishes elite endurance athletes from the nonathletic population, is improved skeletal muscle capacity for oxygen consumption [as typically assessed by maximal oxygen uptake (V&#775;O_2max) determination]; this in turn is a direct result of higher mitochondrial content (37)."

Edited by lostfalco, 14 October 2013 - 04:01 AM.

[lostfalco]

[lostfalco]

[lostfalco]

Can we optimise the exercise training prescription to maximise improvements in mitochondria function and content?

http://www.ncbi.nlm....pubmed/24128929

"The results of cross-sectional studies, as well as training studies involving rats and humans, suggest that training intensity may be an important determinant of improvements in mitochondrial function (as determined by mitochondrial respiration), but not mitochondrial content (as assessed by citrate synthase activity). In contrast, it appears that training volume, rather than training intensity, may be an important determinant of exercise-induced improvements in mitochondrial content. "