I understood that Epithalamin was discontinued due to raw material shortage, however it is still available in capsule form as Endoluten, whose price (high) is likely a reflection of there being limited raw material available,
Regarding a Group Buy custom synthesis of Thymogen, there might be a big technical issue to contend with:
- Genuine Thymalin is produced/packaged as freeze dried powder which requires reconstitution
- Genuine Prostatilen is produced/packaged as freeze dried powder which requires reconstitution
- Genuine Cortexin is produced/packaged as freeze dried powder which requires reconstitution
- BUT, genuine Thymogen is produced/packaged as a solution ready for injection
- AND, unlike other short Peptide Bioregulators, Thymogen isn't available in capsule form
Why? Why wouldn't genuine Thymogen (produced by the same Russian company that produces Prostatilen) also be produced/packaged as freeze dried powder like all the others? It would surely be better for shelf-life.
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A: because short Peptide Bioregulators, particularly those shorter than 4 amino acids in length, are very hygroscopic (attractant of moisture - even minute amounts in the air).
Even if it's produced under GMP conditions in a humidity/moisture controlled environment it will be very difficult to freeze dry (so as to be totally dry) the bulk powder synthesized in order to weigh it to determine the total quantity/weight of peptide actually synthesized.
And then, even if this is successful, in the time it takes to take it out of the freeze dryer and properly/precisely weigh and then bag [for storage] (if not being immediately reconstituted, aliquoted, and freeze dried) the bulk powder synthesized, it will attract moisture from the atmosphere.
Hence why the Russian company which produces genuine Thymogen produces it as an already-reconstituted solution ready for injection. And if you're going to produce it in this form, then you need to be certain it is sterile.
I had Cartalax and Vesugen (both 3 amino acids long) synthesized and it was a nightmare. 2 back to back rounds of 4 days freeze drying and the bulk powders could still not be completely dried.
In the end, to achieve a dry bulk powder, they had to add a known quantity of Trehalose (another excipient like Mannitol, but far more moisture absorbent) to the bulk peptide.
I also had Pinealon synthesized (also 3 amino acids long) and for it too it was also difficult to completely freeze dry the total quantity synthesized to a dry bulk powder, but unlike Vesugen and Cartalax it did not require any moisture absorbing excipient to be added to the total quantity of peptide synthesized in order to achieve a dry bulk powder when freeze dried. It just took an inordinately long time in the freeze drier.
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BUT, the very interesting thing was, that according to various online peptide property calculation tools, Pinealon was supposed to be the most hydrophilic/hygroscopic of all three of them, yet it was the only one of the three which could be freeze dried to a dry bulk powder without the addition of a moisture absorbing excipient to the total quantity of peptide synthesized.
However, those online tools do say that hydrophilicity/hygroscopicity calculations are just theoretical, and that the actual properties in this regard can only be properly determined by experimentation.
Obviously the known quantity of excipient which had already been added to the total quatity of peptide synthesized in order to achieve a dry bulk freeze dried powder (as verified by moisture content test) of Vesugen and Cartalax then had to be taken into consideration when it came to the ultimate reconstitution, aliquoting, and freeze drying into vials.
I am sure that Pivotal Bioscience did not make any real money out of those orders from me because of all the frigging around and extra processes which were required! hahaha!
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If you're going to try to have produced the short Peptide Bioregulators as freeze dried powder, then you have to be careful because a supplier which doesn't give a toss about quality will just weight the bulk peptide powder after freeze drying and tell you there is, say, 1 gram, but 40% of the weight might be water content, and so you'll pay for, say, 1 gram, but only really get 600 mg.
And you can't really tell whether a peptide has a high water content by its appearance. It must be tested, which is yet another reason to use a reputable source to do proper testing, which despite what some posters say, does not in fact cost the earth.
According to online peptide calculation tools, Thymogen contains 1 Hydrophilic amino acid (Glu) and 1 Hydrophobic amino acid (Trp), so who knows how Hygroscopic it will be in reality.
But if the Russian OE producer of Thymogen is not producing it as a freeze dried powder like they are Prostatilen, then it suggests that its Hygroscopicity is an issue.
Edited by pure, 28 March 2016 - 01:36 AM.