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NGF spray

nootropic ngf

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#601 resveratrol_guy

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Posted 21 June 2016 - 02:04 PM

what happened to this group buy

There is no group buy because it has become clear that we can obtain this compound directly with minimal cost overhead.

Finally, after almost a year, I got back on betaBGF last week. This time, I decided to pay for dry ice shipping to see if the effects were any different. As soon as it arrived, I make a fresh nasal spray batch, and dripped in about 4 ug.

Bear in mind, this is the first dose I've had since my surgery. So it's interesting that about half an hour later, the area between my chin and my lower lip started feeling cold, as though I had applied toothpaste to the area. This area had lost much of its sensation as a result of the surgery, and has only partially recovered, so this sort of response makes some sense. I may have dripped some of the nasal spray on it by accident during the dosing process.

But about 20 minutes later still, while I was sitting down working on other things, I started to feel the same "toothpaste" sensation on the roof my mouth toward the front. This is the other major area which had lost much of its sensation as a surgical side effect. It got severe enough to become an annoyance, and only subsided after a couple of hours. Perhaps it occurred due to nasal drainage, or perhaps both symptoms were due to betaNGF which had entered the blood stream and migrated to the affected sites. Either way, especially in the second case, the effect was unmistakable.

In the several days since, on every day but one, I applied a single spray in each nostril, rather than dripping, in exactly the same manner of application as normal nasal spray. The downside of this practice is obviously that the olfactory bulb gets much less exposure than via the drip method, so if it works at all, it must be mostly due to diffusion into the tissues near the brain and the circulation in general. Even if we assume that it cannot cross the BBB, it can definitely cross in places where that vascular network is ruptured. With enough microvascular damage, for instance, it ought to be able to enter the brain easily. (Having said that, I think the data shows that it crosses the BBB everywhere, as previously discussed.)

I estimate that the switch in technique has knocked down my dose by roughly a factor of 10. Since doing so, I have not yet experienced a repeat of the toothpaste sensation. However, I have once again started to experience very vivid dreaming. As before, it's not merely clear and realistic. It's logical, in the sense that the laws of physics seem to be followed just as in the real world, and people behave in the way that one would expect in a modern society. For example, I dreamed of a place where I drove somewhere to get something, then drove back later. On my return, I took the opposite side of the same road, and had to make a U turn back to my destination, in a manner consistent with real road networks; these are not "LSD" episodes with flying monkeys and rainbow colored horses. Suffice to say, though, that none of this means that your dreams will be uplifting or reassuring. Mine seem to have been quite the opposite for the most part.

 

This is not to say that betaNGF somehow causes panic or anxiety; the science suggests very much the opposite. Even in my sober and at times terrifying dreams, I would not characterize my reactions as such. And while awake, I've noticed that several minutes after a spray dose, my whole body seems to slow down and relax with no effort on my part. It's a subtle effect, though, which might well be placebo. I'm generally very calm as it is, so I'm not the ideal candidate to perform such a test. It would be interesting to see what more anxious people experience in this regard, although in good faith I cannot recommend that anyone copy my uncontrolled human experiment.

 

In sum, these observations strengthen my hypothesis that "nerve growth factor" is a misnomer. It should be called "neuron stem cell activating factor", as neuron growth is merely a secondary and very longterm consequence of its actions. I should add that the dry ice didn't seem to enhance the magnitude of the effect, which reinforces my suspicion that this substance is more stable than the documentation would lead us to believe.

At this point, after months on ashitaba chalcone, I would say that betaNGF has a more immediate and obvious impact on brain function. It may just be that chalcone acts over a long period of time, or maybe its effects are quite simply too subtle, apart from its obvious and rapid appetite suppression.
 


Edited by resveratrol_guy, 21 June 2016 - 02:08 PM.


#602 dz93

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Posted 21 June 2016 - 02:23 PM

Let's not forget about GK-2

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#603 dz93

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Posted 22 June 2016 - 03:40 PM

Anyone know why trials for CERE-110 ended? All I can find is that it wasn't as effective as they liked but everything shows it works exactly as intended and is highly tolerated and safe.

#604 resveratrol_guy

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Posted 26 June 2016 - 10:59 PM

Anyone know why trials for CERE-110 ended? All I can find is that it wasn't as effective as they liked but everything shows it works exactly as intended and is highly tolerated and safe.

 

It was not effective. This is sort of unsurprising because apparently AD brains tend to have more NGF activity than normal ones, probably because neural stem cells (NSCs) are trying desperately to sustain the neuron population. But this is all too little too late in a toxic neurological environment.

 

I suspect that combining NGF gene therapy of this sort with various other interventions, such as TERT gene therapy and dietary modifications, would actually work in moderate states of disease. But you know how the system works: we just try monotherapies in isolation, then throw most of them in the garbage can, or charge astronomical sums for those which are slightly effective.

 

So it's back to diet and lifestyle, for now. In the long term, personally, I would rather get TERT than NGF gene therapy, because I don't want to overtax my NSCs with neurogeneration if TERT can suppress the plaque accumulation enough to hold the cell death rate down to a small enough level such that normal NGF secretion can compensate for it.



#605 resveratrol_guy

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Posted 27 June 2016 - 02:07 AM

Also, with respect to betaNGF, even when spraying as opposed to dripping it, I seem to experience the tinnitus spike about 20 or 30 minutes later. This evening, for instance, I took 2 sprays in each nostril then settled down to work. I was quite startled when the spike hit, wondering for a moment what I had done wrong in my diet to cause such an event, only to realize seconds later that it was likely due to the spray.

Playground reported a similar experience earlier in this thread.

What this means to me is that it's definitely getting into the brain. Moreover, the timing is fairly consistent with what is known about exogenous NGF propagation in the rabbit brain (2 hours from front to rear, if I recall). I take this all to mean that the delay from the olfactory bulb to the auditory cortex is the time stated above. (Of course, it may be true that it's mostly coming via the circulatory system; I suppose the distinction is unimportant.)

But why would any form of NGF trigger tinnitus? Shouldn't NGF relieve it by virtue of repair activation? Perhaps the answer is that central (as opposed to mechanical or ciliary) tinnitus is due to the auditory cortex trying to repair itself, but consistently failing. NGF would logically be expected to stimulate such a repair process, only to end up causing a resurgence in tinnitus.

By contrast, my own mesenchymal stem cell therapy resulted in a marked decrease in tinnitus, probably on account of its antiinflammatory effects on the CNS. Not that I think NGF is inflammatory, but the "G" stands for "growth", so we really shouldn't expect it to be antiinflammatory, either.

On a personal note, I continue to be extremely busy writing software, but I do try to check in at least weekly.


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#606 greyfox

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Posted 16 December 2016 - 05:27 PM

 

what happened to this group buy

There is no group buy because it has become clear that we can obtain this compound directly with minimal cost overhead.
 

 

 

Where can it be purchased directly? I'm in a bit of a hurry to head to work and skimmed through the past couple pages of the thread but didn't seem to see any discussion of it.



#607 YOLF

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Posted 16 December 2016 - 10:08 PM

 

Anyone know why trials for CERE-110 ended? All I can find is that it wasn't as effective as they liked but everything shows it works exactly as intended and is highly tolerated and safe.

 

It was not effective. This is sort of unsurprising because apparently AD brains tend to have more NGF activity than normal ones, probably because neural stem cells (NSCs) are trying desperately to sustain the neuron population. But this is all too little too late in a toxic neurological environment.

 

I suspect that combining NGF gene therapy of this sort with various other interventions, such as TERT gene therapy and dietary modifications, would actually work in moderate states of disease. But you know how the system works: we just try monotherapies in isolation, then throw most of them in the garbage can, or charge astronomical sums for those which are slightly effective.

 

So it's back to diet and lifestyle, for now. In the long term, personally, I would rather get TERT than NGF gene therapy, because I don't want to overtax my NSCs with neurogeneration if TERT can suppress the plaque accumulation enough to hold the cell death rate down to a small enough level such that normal NGF secretion can compensate for it.

 

 

Sounds like some alternative therapies for psoriasis might help here along with the right stuff to get your cells metabolizing healthily again as cellular turnover is an issue here too. You might try cycloastragenol and/or astragaloside IV while you wait for the HTERT GT to become available. You could also use the turnover while it's still not under control to purge your ECM with alpha hydroxy acids such as pyruvate.



#608 resveratrol_guy

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Posted 18 December 2016 - 06:19 AM

 

 

Anyone know why trials for CERE-110 ended? All I can find is that it wasn't as effective as they liked but everything shows it works exactly as intended and is highly tolerated and safe.

 

It was not effective. This is sort of unsurprising because apparently AD brains tend to have more NGF activity than normal ones, probably because neural stem cells (NSCs) are trying desperately to sustain the neuron population. But this is all too little too late in a toxic neurological environment.

 

I suspect that combining NGF gene therapy of this sort with various other interventions, such as TERT gene therapy and dietary modifications, would actually work in moderate states of disease. But you know how the system works: we just try monotherapies in isolation, then throw most of them in the garbage can, or charge astronomical sums for those which are slightly effective.

 

So it's back to diet and lifestyle, for now. In the long term, personally, I would rather get TERT than NGF gene therapy, because I don't want to overtax my NSCs with neurogeneration if TERT can suppress the plaque accumulation enough to hold the cell death rate down to a small enough level such that normal NGF secretion can compensate for it.

 

 

Sounds like some alternative therapies for psoriasis might help here along with the right stuff to get your cells metabolizing healthily again as cellular turnover is an issue here too. You might try cycloastragenol and/or astragaloside IV while you wait for the HTERT GT to become available. You could also use the turnover while it's still not under control to purge your ECM with alpha hydroxy acids such as pyruvate.

 

Psoriasis? I think you meant that reply for another thread.


 

Where can it be purchased directly? I'm in a bit of a hurry to head to work and skimmed through the past couple pages of the thread but didn't seem to see any discussion of it.

 

Sinobiological, or one of their distributors listed on their website.



#609 YOLF

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Posted 18 December 2016 - 07:08 AM

Well, I was thinking that since psoriasis is about excessive turnover of skin cells that there might be similar underlying mechanisms to slowing the two diseases. P53 activators for instance, work well, or even modulating telomere lengths, senolytics offer improvement also. And the alternative therapies aren't yet well understood.



#610 Mr. Olive Oil

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Posted 09 December 2017 - 06:34 AM

On the Sinobiological website under Distributors I see USA on the map but no actual link to a USA distributer. That is unfortunate.

Perhaps Sinobiological directly sells to the United States... I'll have to check on that.


Edited by highlightfocus, 09 December 2017 - 06:35 AM.


#611 YOLF

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Posted 09 December 2017 - 03:23 PM

I think for the next group buy, we should get a plasmid for this made, perhaps at the-odin so we can just start manufacturing this stuff ourselves.

 

So what was the outcome? Did people get younger? Iirc, the naked mole rats live much longer than other rodents b/c they make an excess of a NGF.



#612 thompson92

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Posted 28 May 2020 - 02:04 AM

https://content.iosp...ports/adr180055

 

Long-Term Non-Invasive Treatment via Intranasal Administration of Nerve Growth Factor Protects the Human Brain in Frontotemporal Dementia associated with Corticobasal Syndrome: A Pilot Study
Background:

Nerve growth factor (NGF) is known for playing a critical protective role on a number of brain neurons in mammals, including humans. NGF can be delivered to the CNS via nasal route and has a neuroprotective action in case of neurodegenerative diseases.

Objective:

The aim of this study is to investigate for the first time whether purified NGF can play a neuroprotective role on human brain neurons affected by neurodegenerative diseases when administered via nasal route.

Methods:

Two female patients, both affected by frontotemporal dementia (FTD) associated with corticobasal syndrome (CBS) at different stages of disease progression, received a daily intranasal NGF spray for one year. Clinical/neurological aspects were observed over time. The follow-up study was performed using 18 FDG PET.

Results:

This case study seems to demonstrate that IN-NGF slows down the common decline caused by FTD/CBS.

Conclusions:

These findings suggest the potential neuroprotective role of IN-NGF administered in case of neurodegenerative diseases.

 
 
NGF purification and administration

The NGF was purified in our laboratory from submaxillary salivary gland of the adult male mice, following Bocchini and Angeletti method and stored at –70°C until used [59]. Patients received 10 μl of NGF (concentration 200 μg/ml) dissolved in physiological saline solution daily. 5 μl per each nostril daily (via nasal spray) for a 1-year period (range 12–18 months). For further details of purification see: Lambiase et al. [60].

 
 

 



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#613 hotnerds26

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Posted 07 April 2024 - 12:36 AM

I feel I have a moral obligation to mention, that NGF is found in considerable quantities in seminal fluid.

 

Recent studies have identified NGF (previously known as Ovulation-Inducing Factor) in seminal fluid as the main causal agent in Llamas to induce ovulation. A comparable dose of injected NGF to trigger ovaluation was around-above 100-200mcg(!). Llamas roughly have the same ejaculatory volume as humans. Current evidence indicates that the ovulating effect of NGF is not due to localized but systemic action, which would require similarly high dosages to be present.

 

Unlike Llamas, bovines are spontaneous ovulators like humans and NGF levels were found to be about 10x lower per same volume. Due to genome sequence comparison, it is highly likely that NGF is present in the seminal fluid to some degree in all mammals. NGF supports sperm proliferation and various immunologically relevant processes. It has been suggested to use blood serum NGF for bovines as a superior indicator for sperm quality. Although human data is totally lacking, bovine sperm was used as representative sample for spontaneous ovulators like humans in other studies. There is no evidence to assume, that it wouldn't as well contain NGF in similar magnitudes.

 

It has been speculated that semen has antidepressant properties in scientific studies:

 

 

Among women who “always” or “usually” used condoms, about 20% reported suicidal thoughts, but among those who used condoms only “sometimes,” the figure was much lower at 7%. Among women who “never” used condoms, only 5% reported suicidal thoughts

 

Semen retention is a well known and ancient practice, to increase mental and spiritual powers. I can personally attest, that it has extremely powerful mental effects. Those can very well cause and resemble the manic states that people frequently describe online from Dihexa. It could be true that this is actually caused / aggravated simply by high quantities of NGF from reabsorbing seminal fluid.

 

Obviously NGF would also be well absorbed from rectal administration.

 

pubmed.ncbi.nlm.nih.gov/27762054/

pubmed.ncbi.nlm.nih.gov/12049024/


Edited by hotnerds26, 07 April 2024 - 01:01 AM.






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