40 mg is overdose. Stick to 20mg/d max preferably in 2x10mg AM and PM.
I know it's a high dose, but others didn't get much in the way of results at lower doses. The necessary dose would seem to be about 5mg or less. However, this paper suggests a dose of 100-200 mgs in humans, and Dr. Pickart recommended 75mg 3x/wk at one time.
Based on our studies, in which GHK was injected intraperitoneally once daily to induce systemic wound healing throughout the body, we estimate about 100–200 mgs of GHK will produce therapeutic actions in humans. But even this may overestimate the necessary effective dosage of the molecule. Most cultured cells respond maximally to GHK at 1 nanoM. GHK has a half-life of about 0.5 to 1 hour in plasma and two subsequent tissue repair studies in rats found that injecting GHK intraperitoneally 10 times daily lowered the necessary dosage by approximately 100-fold in contrast to our earlier studies
From the rat study:
The total peptide dosage is about 2.2 μg/kg or, if scaled for the human body, about 140 μg per injection with 10 treatments per day
That works out to a dose of 140mg/day if given in a single injection.
This paper also suggests a high dose.
Strong systemic wound healing was induced in pigs at about 1.1 mg GHK-Cu per kilogram body weight which would correspond to about 75 mgs in humans. This is about 300-fold below GHK-Cu's toxic action (lowering of blood pressure).
So 40 mg may or may not be unnecessarily high, but it shouldn't be a toxic overdose.
At any rate, I've been injecting 20mg/day and my skin is still more sensitive, but the rash is gone. I will continue like this for now. I try to split it into 2 injections, but sometimes I do it in one. I would do 10 injections, but that's not feasible.
Edited by bi0hacker, 05 April 2021 - 06:06 AM.