821 replies to this topic

[Logic]

But what a coincidence we're dealing with a supplier who is so remiss as to not have mentioned/addressed this quite important aspect (Mannitol) from the outset?

I can see the above "1." and 2." has been presented/structured in such a way as to suggest that they mentionend Mannitol first, and I just "happened" to raise the point a few minutes afterwards afterwards.

This is BS.

But bigger BS still is: ("TLR actually wanted to ask which would be preferred but did not wish to have any complications/arguments ensue."). hahahaha!! Yeah, sure they did. Total Rubbish!!

I don't appreciate the attempt to try and cover their as_es.

Obviously I mentioned Mannitol first and in doing so they have realized they've put their foot in it (in terms customers impression of their credibility and competency) by not mentioning it at the outset as they should have if they were competent, remotely technically knowledgeable, and anything more than some amateurs operating out of their bedroom, who at times obviously copy and paste (verbatim) technical advice they have obtained from their supplier or another external source in reply to more technical questions raised, while letting us punters think it's their in-house knowledge base which has imparted the information.

Since they say with authority ("TLR is open to running it either way") that it can be lyophilized without Mannitol (because we are dealing with a supplier who possesses so much technical knowledge, NOT!!), then they should lyophilize just native peptide and see what happens. I dare them.

And when they do (and see the result) we can then query them on the above two bracketed statements they've made and ask them how on earth they could possibly have said such things IF they were anything more than amateurs operating from their bedroom.

Did someone who dropped out of high school at the second year author the below?:

"To make a best lyophilized powder (best drug for peptide types) it is best to add mannitol to the powder. This is standard procedure. Lyophilization is obviously quality appearance and all. It can be done without it perhaps, though it may not be as 100% 'on point'"

IF I am going to contemplate putting a research compound into my body, I at least want obtain it from a supplier who has the grammar skils of someone older than 10.

Harsh perhaps?

I think not because we're talking about other peoples health here. There is simply no room for jackass incompetence and amateurism, because one day it will lead to someone getting someone hurt.

 

Hell! True to you nic! 

 

I think they  were trying to avoid just this sort of thing.  I was.

The quoted bit is from a very early Email, before TLR was aware that they could be a little more technical when talking to me. 

It was I who thought it best to stick with exactly what was used in the studies, so its more my fault that this was not brought up earlier.
I still do as the unused product will stay in the freezer, but wonder about degradation during postage?

 

Plz let us know your thoughts on lyophilization with Mannitol as this is the actual urgent question at hand atm.

Edited by Logic, 02 February 2015 - 01:16 PM.

[pure]

I've never read a study which specifically mentions with/without mannitol. Effectively it's considered an inactive excipient.

So, I don't think you can assume that mannitol wasn't used in a study just because it isn't mentioned.

Given it's a 50/50 blend, I think it is best if the lab in China producing it advises whether or not Mannitol should be incorporated.

[Logic]

I've never read a study which specifically mentions with/without mannitol. Effectively it's considered an inactive excipient.
So, I don't think you can assume that mannitol wasn't used in a study just because it isn't mentioned.
Given it's a 50/50 blend, I think it is best if the lab in China producing it advises whether or not Mannitol should be incorporated.

Thx. Much appreciated.
I'm sure TLR is watching and will do whatever is best while keeping how we plan to use the product (sublingual and topical) in mind.

[niner]

It doesn't matter if there's mannitol in it, as long as we know the concentration of the peptide and complex.  It's probably best if there is mannitol in it, so that it's easier to handle sub-milligram amounts of the peptide.  Basically, it has to be cut with something in order to measure it out (even if the something is water), and mannitol is pretty benign.   Is TLR's lab in China?  That wasn't my impression.  It would be nice to see a picture of their facility, if they'd be up for that.

[Logic]

It doesn't matter if there's mannitol in it, as long as we know the concentration of the peptide and complex.  It's probably best if there is mannitol in it, so that it's easier to handle sub-milligram amounts of the peptide.  Basically, it has to be cut with something in order to measure it out (even if the something is water), and mannitol is pretty benign.   Is TLR's lab in China?  That wasn't my impression.  It would be nice to see a picture of their facility, if they'd be up for that.

 

Thx Niner.  yet again! 

 

[Heisenburger]

I’ve pre-ordered one gram from TLR. I’ve gathered that 200 micrograms is the dose I should be aiming for. So far, only sublingual and subcutaneous administration has been suggested. Any reason why I can’t simply dissolve it in buffered saline and administer it as a nasal mist?

[Logic]

Synergistic additive supps with GHK/GHK-Cu
http://www.longecity...with-ghkghk-cu/

 

Let us know your reasons why you are going to take GHK/GHK-Cu and what you think would synergise or be additive to its effects.

[echocharlie]

Dosage and Method of Administration

 

The protocol linked http://www.hindawi.c...ri/2014/151479/ seems to indicate that a favorable dose for human studies would be in the 100-200mg range.

 

"Based on our studies, in which GHK was injected intraperitoneally once daily to induce systemic wound healing throughout the body, we estimate about 100–200 mgs of GHK will produce therapeutic actions in humans."

 

I'm assuming that IV administration would be the corresponding protocol in humans and have similar efficacy, but 100mgs is higher than the 100mcgs I've seen elsewhere in this thread, and would mean that a 30-40 day trial would require 4g GHK/GHK-Cu; however, the paper goes on to say:

 

"But even this may overestimate the necessary effective dosage of the molecule. Most cultured cells respond maximally to GHK at 1 nanoM. GHK has a half-life of about 0.5 to 1 hour in plasma and two subsequent tissue repair studies in rats found that injecting GHK intraperitoneally 10 times daily lowered the necessary dosage by approximately 100-fold in contrast to our earlier studies [38, 65]."

 

Lowering the dosage 100-fold would certainly decrease the cost of testing the protocol (and get the dosage down into that 100mcg range) but 10x/daily IV dosing would be inconvenient to say the least. This brings me to my questions:

1. Is anyone planning on running the protocol at ~100mcgs IV 10x/daily?
2. Is anyone planning on running the protocol at 100mgs IV qid?
3. What other dosages are experimenters planning to run?
4. What other administration methods are experimenters planning to use (oral, sublingual, inhaled, topical with or without DMSO?)
5. Is there any evidence (other than similarity with other tripeptides) that oral or sublingual administration would be effective?
6. If so, approximately how efficient would other administration modalities be?
7. How long are experimenters planning to run the protocol?
8. How are experimenters planning to measure the effects?

Edited by echocharlie, 06 February 2015 - 10:59 PM.

[Heisenburger]

I’ve been fiddling around with an empty Afrin bottle in anticipation of my upcoming experiments. It seems to administer a metered dose of exactly 0.10 milliliters. I filled the empty bottle with ten milliliters of water using a cheap transfer pipette, and it gave exactly 99 squirts and then sputtered on the hundredth squirt. This should make the mixing math easy.

[niner]

 

Dosage and Method of Administration

 

The protocol linked http://www.hindawi.c...ri/2014/151479/ seems to indicate that a favorable dose for human studies would be in the 100-200mg range.

 

"Based on our studies, in which GHK was injected intraperitoneally once daily to induce systemic wound healing throughout the body, we estimate about 100–200 mgs of GHK will produce therapeutic actions in humans."

 

I'm assuming that IV administration would be the corresponding protocol in humans and have similar efficacy, but 100mgs is higher than the 100mcgs I've seen elsewhere in this thread, and would mean that a 30-40 day trial would require 4g GHK/GHK-Cu; however, the paper goes on to say:

 

"But even this may overestimate the necessary effective dosage of the molecule. Most cultured cells respond maximally to GHK at 1 nanoM. GHK has a half-life of about 0.5 to 1 hour in plasma and two subsequent tissue repair studies in rats found that injecting GHK intraperitoneally 10 times daily lowered the necessary dosage by approximately 100-fold in contrast to our earlier studies [38, 65]."

 

Lowering the dosage 100-fold would certainly decrease the cost of testing the protocol (and get the dosage down into that 100mcg range) but 10x/daily IV dosing would be inconvenient to say the least. This brings me to my questions:

1. Is anyone planning on running the protocol at ~100mcgs IV 10x/daily?
2. Is anyone planning on running the protocol at 100mgs IV qid?
3. What other dosages are experimenters planning to run?
4. What other administration methods are experimenters planning to use (oral, sublingual, inhaled, topical with or without DMSO?)
5. Is there any evidence (other than similarity with other tripeptides) that oral or sublingual administration would be effective?
6. If so, approximately how efficient would other administration modalities be?
7. How long are experimenters planning to run the protocol?
8. How are experimenters planning to measure the effects

 

Yeesh.  Pickart et al. aren't entirely making sense here.  First they say 100-200 MILLIGRAMS(!) then later they say:

 

 

The most likely effective dosage of GHK was given to rats for healing bone fractures. This mixture of small molecules included Gly-His-Lys (0.5 μg/kg), dalargin (1.2 μg/kg) (an opioid-like synthetic drug), and the biological peptide thymogen (0.5 μg/kg) (L-glutamyl-L-tryptophan) to heal bones. The total peptide dosage is about 2.2 μg/kg or, if scaled for the human body, about 140 μg per injection with 10 treatments per day [38, 65].

 

The share of that 140 ug (times ten) that is GHK is ~35 ug * 10, or about a third of a milligram.  It's not clear what accounts for a nearly three order of magnitude difference in dose, but it doesn't give me a lot of confidence that this is well sorted out.  I wouldn't take 200 mg of this stuff if you paid me.  Not until I see some evidence that it is safe and effective, at least.

 

It is possible for tripeptides taken orally to be absorbed in the gut without hydrolysis.  Obviously it would not be as effective as an iv or ip route, but it might work.  I don't know how you'd figure out the proper dose.  You'd need a way to get a blood level.  I don't have a good feeling about delivering a peptide sublingually.  I think that would just end up being an oral dose, along with some unnecessary exposure to saliva enzymes.  (That's just my gut feeling, no data.)

Edited by niner, 07 February 2015 - 03:33 AM.

[Heisenburger]

I don't know how you'd figure out the proper dose. 

 

 

I’m just gonna wing it. Twenty milligrams per day for 50 consecutive days and just guage the subjective results. This is probably the silliest LE experiment I have ever run on myself, because I really have absolutely no idea what I’m doing. So I’m going to dissolve 100 milligrams of the stuff in 10 milliliters of buffered saline and snort twice (once in each nostril) ten times a day until the gram is exhausted in seven weeks or so. Needless to say, I’ll report back.

 

[Arcanist]

Hi guys, rather new LC-member here - I'm about to read up this thread and intent to try out the peptide. I feel like my face and looks are deterioating by the day for some time now. It's horrible, there's something going on with my connective tissue- eyes/cornea as well. Will report back here in regular intervals

[echocharlie]

Yeesh.  Pickart et al. aren't entirely making sense here.  First they say 100-200 MILLIGRAMS(!) then later they say:

 

 

The most likely effective dosage of GHK was given to rats for healing bone fractures. This mixture of small molecules included Gly-His-Lys (0.5 μg/kg), dalargin (1.2 μg/kg) (an opioid-like synthetic drug), and the biological peptide thymogen (0.5 μg/kg) (L-glutamyl-L-tryptophan) to heal bones. The total peptide dosage is about 2.2 μg/kg or, if scaled for the human body, about 140 μg per injection with 10 treatments per day [38, 65].

 

The share of that 140 ug (times ten) that is GHK is ~35 ug * 10, or about a third of a milligram.  It's not clear what accounts for a nearly three order of magnitude difference in dose, but it doesn't give me a lot of confidence that this is well sorted out.  I wouldn't take 200 mg of this stuff if you paid me.  Not until I see some evidence that it is safe and effective, at least.

 

It is possible for tripeptides taken orally to be absorbed in the gut without hydrolysis.  Obviously it would not be as effective as an iv or ip route, but it might work.  I don't know how you'd figure out the proper dose.  You'd need a way to get a blood level.  I don't have a good feeling about delivering a peptide sublingually.  I think that would just end up being an oral dose, along with some unnecessary exposure to saliva enzymes.  (That's just my gut feeling, no data.)

 

 

My reading of that would be that the quoted protocol utilized a mixture that included Gly-His-Lys together with dalargin and thymogen specifically targeted for bone healing. They then extrapolated a scaled dosage for humans @ 140 μg delivered 10xdaily IV (human dosing IP isn't practical, so I'll just assume IV). This seems to follow from their previous assertion that due to the half-life, multiple doses maintained optimal levels with smaller total dosing.

 

In the absence of dilargin and thymogen (and absent targeting bone healing) they still seem to be asserting (elsewhere in the paper) that roughly 100-200 μg administered 10x daily would be a comparable human dose. This still doesn't answer the question of how to objectively measure any effects and/or side-effects (although none were reported in the studies). What other factors that could be objectively measured would provide markers for efficacy and dose-efficiency?

 

Put another way, what are we trying to measure, and how do we measure whether the dosage and administration protocol are ultimately effective?

 

You also state that you wouldn't take 200mgs of GHK/GHK-CU without further evidence that it is safe and effective. The study doesn't indicate any side effects at the stated dosages. Granted, it is a single-study in a rat model, but what potential contraindications would you be watching for that would cause you to abort testing?
 

 

I don't know how you'd figure out the proper dose. 

 

 

I’m just gonna wing it. Twenty milligrams per day for 50 consecutive days and just guage the subjective results. This is probably the silliest LE experiment I have ever run on myself, because I really have absolutely no idea what I’m doing. So I’m going to dissolve 100 milligrams of the stuff in 10 milliliters of buffered saline and snort twice (once in each nostril) ten times a day until the gram is exhausted in seven weeks or so. Needless to say, I’ll report back.

 

 

What subective measures are you looking for to determine whether the peptide is having any effects / side-effects? Are you planning to titrate dosage until some particular measure is reached? What if anything would cause you to change dosage or abort testing altogether?

[niner]

You also state that you wouldn't take 200mgs of GHK/GHK-CU without further evidence that it is safe and effective. The study doesn't indicate any side effects at the stated dosages. Granted, it is a single-study in a rat model, but what potential contraindications would you be watching for that would cause you to abort testing?

 

You'd want a full complement of blood work-- the usual metabolic panels, blood counts, liver enzymes, kidney parameters, heart function, anything that might indicate that something was going south.

[echocharlie]

This paper by Pickart, Vasquez-Soltero, and Margolina seems to be a bit more thorough treatment of GHK:

 

http://www.ncbi.nlm....les/PMC3359723/

 

There appears to be a long track record of safe usage in humans - at least in skincare (implying topical application):

 

 

The human copper-binding tripeptide glycyl-L-histidyl-L-lysine (GHK) is a compound with a long history of safe use in wound healing and antiaging skin care. Since its discovery in 1973, almost four decades of extensive research have established its diverse beneficial actions in many organs and tissues including nervous tissue, skin, intestine, bone, and blood vessels.

 

GHK is a natural consituent of human plasma, and levels decline with age. We might look at internal supplementation to replace youthful levels.

 

 

 

Human peptide GHK was isolated in 1973 as an activity in human plasma that caused old human liver tissue to synthesize proteins like younger tissue [10]. In human plasma GHK is present at about 200 micrograms/liter in men of age 20–25 but declines to 80 micrograms/liter by age 60–80.

 

Pickart et al state that oral adminsitration would require encapulation into lipsomes, and offers a human dose extrapolated from a wound healing study in pigs. They also provide a rough indication of toxic action (hypotension) and levels of GHK that induced toxicity (330 mg / kg), then go on to once again say that even lower doses might still be therapeutic.

 

 

The peptide could be administered intravenously or orally when encapsulated into liposomes. Strong systemic wound healing was induced in pigs at about 1.1mg GHK-Cu per kilogram body weight which would correspond to about 75 mgs in humans. This is about 300-fold below GHK-Cu's toxic action (lowering of blood pressure). Much lower dosages may also be effective since GHK-Cu's actions on cells generally occur at a 1 nanomolar concentration [68].

 

GHK peptide vs. GHK-Cu copper complex are also discussed in the conclusion:

 

 

Even though it is not always possible to distinguish between activity of GHK peptide and its copper complex GHK-Cu, we strongly believe that for the future therapeutical applications GHK-Cu should be used. Numerous studies demonstrating pleiotropic health promoting and antiage activity of the GHK-Cu peptide together with recent studies revealing gene regulating activity of GHK suggest that this compound may belong to a class of epigenetic modifiers capable of exhibiting broad protective and restorative actions, reducing harmful epigenetic changes caused by environmental perturbagens. The GHK-Cu peptide should be considered a promising neuroprotective agent capable of preventing the development of common age-associated neurodegenerative disorders.

 

Edit: Clarity, added section for safe usage and presence/levels in human plasma.

Edited by echocharlie, 09 February 2015 - 04:01 PM.

[johnross47]

Any word on when we might get our orders of ghk?

[Heisenburger]

Good question. I'm still waiting for mine. This is somewhat annoying, as my account has already been debited, and the e-mail I received from TLR on February 5 clearly indicated that it would arrive by the 12th.

 

[Logic]

I've contacted TLR. They will be posting here shortly.
Its nothing serious. but things have been delayed due to an oversight.
I'll let them explain.

[pure]

hahahah! I hate to say 'I told you so', but... from my post 238 in which I raised for the very first time the crucial issue of with/without Mannitol (which should have been addressed by the [in]competent supplier on day 1):

"..if they were competent, remotely technically knowledgeable, and anything more than some amateurs operating out of their bedroom.."

"There is simply no room for jackass incompetence and amateurism.."

 

Further affirmation of this, what-should-be now be obvious to all, aspect about the supplier is that they don't even possess the professionalism to contact customers and inform them of their "oversight".

Rather, it takes customers to report their failure, before someone (not even them) makes mention of it on their behalf.

Will be interesting to see what story they invent to try and cover their ass_s.

 

But despite all this, no doubt there will still be those who are prepared to put into their bodies what 'the guys in the garage' are peddling. Hilarious.

I suggest that someone independent of the group buy finds an analytical laboratory to test the final compound for Identity, Purity, Microbial's, etc., and a different person (also unconnected with the group buy) deals with the lab and reports the results.

 

I've contacted TLR. They will be posting here shortly.
Its nothing serious. but things have been delayed due to an oversight.
I'll let them explain.

 

Edited by pure, 20 February 2015 - 07:01 AM.

[Logic]

hahahah! I hate to say 'I told you so', but... from my post 238 in which I raised for the very first time the crucial issue of with/without Mannitol (which should have been addressed by the [in]competent supplier on day 1):
"..if they were competent, remotely technically knowledgeable, and anything more than some amateurs operating out of their bedroom.."
"There is simply no room for jackass incompetence and amateurism.."
 
Further affirmation of this, what-should-be now be obvious to all, aspect about the supplier is that they don't even possess the professionalism to contact customers and inform them of their "oversight".
Rather, it takes customers to report their failure, before someone (not even them) makes mention of it on their behalf.
Will be interesting to see what story they invent to try and cover their ass_s.
 
But despite all this, no doubt there will still be those who are prepared to put into their bodies what 'the guys in the garage' are peddling. Hilarious.
I suggest that someone independent of the group buy finds an analytical laboratory to test the final compound for Identity, Purity, Microbial's, etc., and a different person (also unconnected with the group buy) deals with the lab and reports the results.

Has there ever been a group buy here with out a flame war and people unnecessarily ending up as enemies?
Inevitably the group goes in a direction not liked by some. That's just how things are.

In your case Pure; you were keen on GHK... from a supplier of your choice?
http://www.longecity...ndpost&p=696584
http://www.longecity...ndpost&p=698007

The group decided to go the way it did. That's life.

Your bringing up Manitol
http://www.longecity...ndpost&p=711086
did cause a delay as Myself and TLR waited for feedback from others here, then decided to go with exactly what everyone paid for.

Please don't take a shit on myself and essentially everyone else who decided to buy because things didn't turn out as You would have liked.
This is the 1st time I have organised a 'group buy' and no-one wants it to go well as much as I do.
The way things have worked out has resulted is an unforeseen delay. Shit happens.
Everyone will get what they chose to order. Its just going to take a little longer is all.

[pure]

Huh? I don't care one iota about who the group buys from. I got one quote to contribute to starting the process and another from Active Peptide. I haven't mentioned it at all since.
I'm merely pointing out the obvious, same as I would have done if Active Peptide had been the supplier and it became apparent they were inept amateurs.

 

"Shite" as you say, generally only happens when you're dealing with unprofessional amateurs working out of their garage.

IF they knew what they were doing, they would have addressed the Mannitol issue right at the start, because they would know that GHK is hygroscopic.

But they didn't because they don't know.

 

And on that basis, there is grounds and every reason to have an independent analytical laboratory verify identity, purity, microbials, etc., and have that process managed by someone not connected with the group buy.

Edited by pure, 20 February 2015 - 12:11 PM.

[tepol]

Very interesting substance. I suffer from a few health issues that might, in my unscientific opinion, share a common feature of collagen problems: contractures in both hands, a leaky heart valve and a badly stretched bladder that requires self catheterisation. If it was available I would jump at the chance of trying it.

 

have you been tested for ehlers danlos syndrome ?

[tepol]

Hey everyone. Its the new year (all the best) and time to get this group buy going!

I was hesitant to do this group buy as the postal service here in South Africa is both bankrupt (read cash-flow embezzled) and continuously on strike and just generally too 3rd world to be considered reliable.

Also, I was loath to work with everyone's hard earned cash and perhaps become as popular as baked beans on a bus trip if anything went wrong.

TeamTLR, have offered a rather elegant solution to both of the above issues:

They are willing to produce 30 grams of GHK/GHK-Cu (50/50) 98% at their own cost if the 30 or so interested people here commit to buying a gram each from them at 89.90USD per gram (+ shipping cost, as per selected/desired method). Your gram will arrive in 10 vials of 100 mg per vial.

They will add the item to their website to facilitate easy ordering once the 30 or so people who have expressed interest here confirm that they will be buying the product.
(This will allow everyone to select whichever shipping option they preferred upon checkout)

The GHK/GHK-Cu will be listed on the site at 109.90USD per gram,  but we get a discount code worth 20USD.
The code will be posted here and it will be valid for a period of 10 days from posting.

Here is the list of people who have shown interest either here or via PM:

Katrina >
medicineman > 1gram +
neuralis > 500mg
smithx
johnross47
thedarkbobo > 333mg
xEva
smccomas01 > 1gram
Hebbeh >
nbourbaki > 1gram
smccomas01 > 1gram
pure > got prices from Pivotal Bioscience pure GHK 179 for 5 grams 158 for 10
smithx > worried about copper
pampoenkop
neuralis
micro2000
sthira
Metagene
Soma
Infinite1
PWAIN
Ehvam
ceridwen
gt35r
ta5
zorba990
Volcanic
pleb

Logic

Please post your intention to buy here so that TeamTLR can start production. Once I confirm with them we have reached a 30g 'commitment' (or thereabouts) they will add the item to the website, remit the Code to me to post, and they will commence production.

The lead time from the commitment and subsequent posting of the aforementioned code is approximately 3 weeks.

I hope this meets with everyone's approval?

 

I think I missed the 1st buy , curious to hear how you all get on once you get your orders and whether they'll be 2nd group buy based on your results.

 

tepol

 

 

 

[tepol]

Quick question - what effect would GHK have on genetic diseases ( specifically connective tissue ones like EDS ) , any ideas or rough guesses  ?

[Forty Six & 2]

Greetings All,

 

Many sincerest apologies for the delay within the shipping of the GHK/GHK-Cu 1:1 Blend. Truly we regret to have to relate such.

 

We had need to best optimize the lyophilization, which took a bit longer to coordinate than anticipated. We deemed this essential especially due to international shipping concerns, as well as to supply the most superior overall product.

 

The issue as to the delay was in which we did coordinate such with the idea we would get under the time line of the Chinese New Year for the product to ship out to us on the 12th. However, all shipping closed off quite a few days earlier than the actual CNY and left the shipment as such delayed heavily by the total shutdown of Chinese shipping until February 27^th. We have a long and very solid relationship with this supplier. They were certainly doing their best to do as they thought would best accommodate all aspects, and regrettably did not know the window was to close so soon.

 

As such, we will be looking at a shipping time within the current estimated arrival to be circa two weeks. Again, we do very much regret the long delay and do very much appreciate all gracious patience and understanding.

 

We would also like to announce we are working on something new that we feel is very strong within the scope of anti-aging research, especially within potentials that appear evident as within a synergistic efficacy with co-administration of GHK/GHK-Cu. Hopefully we will have the good news to relay within this shortly.

 

All really indeed looked on track to be within to enable shipping near the estimated lead time, however, indeed those who have experience in the real world know that 'things happen', small glitches can create quite undesirable delays, and it's better to place doing it right over doing it fast most all the time.  Any other questions or inquiries of course we will do our best to answer such promptly.

 

Respectfully,

Forty Six & 2

& TeamTLR 

 

P.S.  

As to tepol's inquiry above, GHK dynamics appear to have an almost panacea-type profile of beneficial effects, that encompass rejuvenation of tissues which would seem to include connective tissues as well.  Now depending on the genetic specific detriments there may be varying degrees of how such may be of benefit, but it appears it likely will only be of benefit, and has no clear indications as I have read to be otherwise within any capacity.  That's a very good thing!

 

Connective tissue related links:

http://www.ncbi.nlm..../pubmed/8227353

http://link.springer...456-5_10#page-1

 

Furthermore, to elaborate on other beneficial effects that have been demonstrated to date, GHK has shown solid indications for as well having positive effects on mood, anxiety, pain, and the list goes on as to so many other beneficial effects.  Certainly further research within this area is of strongest potential.  

 

Apologies again for the delay, as trust we are as eager to get this out to all as anyone is eager to have it arrive for their research.

Edited by Forty Six & 2, 20 February 2015 - 07:25 PM.

[Logic]

Quick question - what effect would GHK have on genetic diseases ( specifically connective tissue ones like EDS ) , any ideas or rough guesses  ?

I don't know Tepol, but it seems worth a try when you look at the effects GHK/GHK-Cu had in tests.
Why not contact TLR and ask if there is any left as they may have had more made than what the group buy ordered for just this sort of occasion and future buyers.

[Logic]

Group buy members:
Please not that the GHK will come in hermetically sealed containers so there is no chance of moisture and bacteria getting in and degrading the lyophilised peptide.
I plan to add bacteriostatic or distilled water and wine to my vials, one at a time, before sublingual and topical use, just as I did with Epitalon.
The alcohol in the wine will protect the peptide from degradation by bacteria long enough to finish the vial.
The other vials will be kept in the freezer until needed.

[Forty Six & 2]

As a quick note, to address the above, there are about 2 lots of 10 x 100mg presently unaccounted for - so for anyone wishing in on this batch kindly note such and this may go to "Out of Stock" status on the site any day within such.

 

We will likely be running another batch shortly after this one, within we feel this is a strong item of requisite research for further investigation toward 'progress and betterment'.  We are as well researching into the development of something even more ambitious related directly to GHK, but that is a long way off and within the early stages of investigation.  This is not what was prior alluded to in the last post, just to be clear.

 

Kindly note, it is best for in vivo animal research to use bacteriostatic water, which is more than sufficient to keep the peptide and solution stable for quite some period, within proper refrigeration and storage.

 

We do have some 30ml vials of Bac Water (0.9% Benzyl Alcohol) for our 'in-house' research and perhaps can make such available upon request.  There was thought to make such available on the site initially, however, we wanted to keep packs small and tidy for our international shipping customers and chose to direct all items and packaging to best accommodate such.

 

[tepol]

As a quick note, to address the above, there are about 2 lots of 10 x 100mg presently unaccounted for - so for anyone wishing in on this batch kindly note such and this may go to "Out of Stock" status on the site any day within such.

 

We will likely be running another batch shortly after this one, within we feel this is a strong item of requisite research for further investigation toward 'progress and betterment'.  We are as well researching into the development of something even more ambitious related directly to GHK, but that is a long way off and within the early stages of investigation.  This is not what was prior alluded to in the last post, just to be clear.

 

Kindly note, it is best for in vivo animal research to use bacteriostatic water, which is more than sufficient to keep the peptide and solution stable for quite some period, within proper refrigeration and storage.

 

We do have some 30ml vials of Bac Water (0.9% Benzyl Alcohol) for our 'in-house' research and perhaps can make such available upon request.  There was thought to make such available on the site initially, however, we wanted to keep packs small and tidy for our international shipping customers and chose to direct all items and packaging to best accommodate such.

 

What temp would you best recommend for long term storage and how long would that exactly last ?

 

Also when do you plan to get 2nd batch ?

 

thanks,

 

tepol
 

[pleb]

Most peptides still in the vial as lyphalised powder can be stored in a freezer and will last a minimum of two years probably longer. After adding bac water preferable if your planning on injecting to one vial at a time leave the remainder in the freezer.
or sterile water and wine if under the tongue via the mucous membrane after dilution it should last at least a month in the fridge. it will start to go cloudy when it gets past its usefully life.
It's not recommended to store the diluted vial in the freezer as repeated freezing and thawing can damage the peptide.