2255 replies to this topic

[Ethic]

Great links and a very personal insight to your main motive regarding NR, thank you for this, Bryan.

 

I want to give you guys something back for all the things I've learned through this forum and it's even NAD+ related.

 

I started smoking pot relatively young and stopped it one and a half years ago, but I still suffered from a light bipolar disorder.

 

Then I stumbled over this article:

Why Aging People Become Depressed, Fatigued, and Overweight

http://www.lifeexten...rweight/Page-01

http://www.lifeexten...rweight/Page-02

 

So recently I stopped Nicotine as well by strengthening my will through vegan Tryptophan supplementation (->Serotonin, Melatonin, Niacin).

 

Interestingly, my mood became very stable, less thoughts about stupid humanity and my circadian rhythm improved significantly.

Previously I stayed up till 03:00 am almost every night and now I get tired when the sun goes down

 

I can highly recommend it to support your soberness or to even become a better human? (dramatic)

 

It's the least common essential amino acid in our food. I think there's more to it than modern science knows to date...a evolutionary reward system anyone?

 

 

This site could be interesting to you guys aswell, it's basically an overview of natural active substances:

 

http://health-notes....com/cancer.html

 

 

 

 

[Logic]

Great links and a very personal insight to your main motive regarding NR, thank you for this, Bryan.

 

I want to give you guys something back for all the things I've learned through this forum and it's even NAD+ related.

 

I started smoking pot relatively young and stopped it one and a half years ago, but I still suffered from a light bipolar disorder.

 

Then I stumbled over this article:

Why Aging People Become Depressed, Fatigued, and Overweight

http://www.lifeexten...rweight/Page-01

http://www.lifeexten...rweight/Page-02

 

So recently I stopped Nicotine as well by strengthening my will through vegan Tryptophan supplementation (->Serotonin, Melatonin, Niacin).

 

Interestingly, my mood became very stable, less thoughts about stupid humanity and my circadian rhythm improved significantly.

Previously I stayed up till 03:00 am almost every night and now I get tired when the sun goes down

 

I can highly recommend it to support your soberness or to even become a better human? (dramatic)

 

It's the least common essential amino acid in our food. I think there's more to it than modern science knows to date...a evolutionary reward system anyone?

 

 

This site could be interesting to you guys aswell, it's basically an overview of natural active substances:

 

http://health-notes....com/cancer.html

 

NAD+ is synthesised via two major pathways in humans; the NAD de novo biosynthesis and NAD salvage pathways...the de novo pathway starts with L-tryptophan...humans stricken with pellagra, a nicotinamide (niacin) deficiency disease, recovered after the addition of tryptophan...

http://humancyc.org/...ject=NADSYN-PWY

 

NAD+ synthesis pathways are categorized into either de novo pathways, which start with the amino acid aspartate or tryptophan, or salvage pathways, which start with nicotinamide (NAM) or nicotinic acid (NA)

http://journals.plos...al.pgen.1001105

 

In all vertebrates, NAD can be synthesized by...de novo synthesis from tryptophan...For the de novo pathway to be completed it is necessary to have adequate riboflavin (vitamin B2), pyridoxyl phosphate (vitamin B6), and ascorbate (vitamin C).

http://www.ncbi.nlm....les/PMC2651433/

 

With increasing age...profiling of blood serum displayed a marked decrease in tryptophan concentration

http://www.longecity...ndpost&p=763338

[ceridwen]

I've been told that tryptophan increases quinolinic acid,a marker for Neuro degeneration.

[Bryan_S]

Mitonuclear communication in homeostasis and stress http://www.nature.co...rm.2016.23.html

 

http://www.nature.com/ mentions Nicotinamide Riboside in 2 papers this month.

 

 

Nuclear DNA damage signalling to mitochondria in ageing

http://www.nature.co...rm.2016.14.html

 

 

 

 

 

Figure 5: Pharmacological interventions in the DNA damage response that may lead to increases in lifespan and healthspan.

close

 

 

 

 

a | Inhibition of poly(ADP-ribose) polymerase 1 (PARP1) by olaparib and other PARP inhibitors has been shown to increase lifespan in model organisms.b | Impaired cellular metabolism can be counteracted by treatment with NAD⁺ precursors…

 

[Bryan_S]

ASBMB AWARD FOR EXEMPLARY CONTRIBUTIONS TO EDUCATION

 

Brenner recognized for devotion to 'cutting-edge education’ and serving the biomedical community

http://www.asbmb.org...plaryEducation/

 

 "Milestones of Brenner’s research include the discovery of the eukaryotic nicotinamide riboside kinase pathway and dissection of the roles of DNA methylation and demethylation in the epigenetic regulation of tumor-suppressor genes."

[Ethic]

Thank you for the links, Logic

I should have Vitamin B2, B6 and C covered (VEG1 Supplement + fruits)

 

An intake of 60 mg Tryptophan result in 1 mg Niacin metabolite

 

http://www.ncbi.nlm....pubmed/15895512

 

So 3500 mg Tryptophan result in 58 mg Niacin if it's converted linearly

 

I've been told that tryptophan increases quinolinic acid,a marker for Neuro degeneration.

 

quinolinate is an intermediate of de novo biosynthesis pathway

 

http://humancyc.org/...ject=NADSYN-PWY

[Ethic]

Mitonuclear communication in homeostasis and stress http://www.nature.co...rm.2016.23.html

 

http://www.nature.com/ mentions Nicotinamide Riboside in 2 papers this month.

 

 

Nuclear DNA damage signalling to mitochondria in ageing

http://www.nature.co...rm.2016.14.html

 

 

 

 

 

 

Figure 5: Pharmacological interventions in the DNA damage response that may lead to increases in lifespan and healthspan.

close

 

 

 

 

a | Inhibition of poly(ADP-ribose) polymerase 1 (PARP1) by olaparib and other PARP inhibitors has been shown to increase lifespan in model organisms.b | Impaired cellular metabolism can be counteracted by treatment with NAD⁺ precursors…

 

 

Woohooo, i see

 

1) Olaparib

2) NR; beta-Hydroxybutyric acid?

3) AICAR (ZMP)

4) Rapamycin; Spermidine

Edited by Ethic, 10 March 2016 - 12:05 AM.

[Ethic]

...

a | Olaparib; Veliparib

b | NR; P7C3; BHBA

c | ???; AICAR (ZMP)

d | Rapamycin; Spermidine

Edited by Ethic, 10 March 2016 - 02:02 AM.

[Bryan_S]

ChromaDex Initiates Second Human Clinical Study on NIAGEN® -The World’s First and Only Commercially Available Form of Nicotinamide Riboside (NR)

- Broader Study Will Build Upon the Successful Results of the First Trial Which Demonstrated NIAGEN^® is a Safe, Effective NAD+ Precursor in Humans -

IRVINE, Calif., March 10, 2016 (GLOBE NEWSWIRE) -- ChromaDex Corp. (OTCQX:CDXC), an innovator of proprietary health, wellness and nutritional ingredients, that creates science-based solutions to dietary supplement, food and beverage, skin care, sports nutrition, and pharmaceutical products, announced today the initiation of the second human clinical trial for its patented ingredient, NIAGEN^® (Nicotinamide Riboside - NR).   

The second human clinical trial will build upon the results of the Company’s first human clinical trial announced in February 2015 which demonstrated that a single oral dose of NIAGEN^® is a safe, effective nicotinamide adenine dinucleotide (NAD+) precursor in humans.  The results of that study constituted a significant milestone as it showed for the first time an increase in NAD+ in humans has been demonstrated through NR supplementation.

The second human clinical trial will be a randomized, double-blind, placebo controlled parallel study in 140 healthy adults ranging in age from 40-60, which will examine the benefits of NIAGEN^® if taken daily for eight consecutive weeks.  The outcome will provide key information on the effective dose range of NIAGEN^® to increase the mitochondrial co-enzyme NAD+ and NAD+ metabolite concentrations in the body.   

Additional endpoints to be investigated include: C-reactive protein, total cholesterol, LDL, HDL and triglycerides and amino acid panels, NAD+ levels in muscle, mitochondrial biomarkers, and the effects of NR on Resting Metabolic Rate.

Frank Jaksch Jr., founder and CEO of ChromaDex, commented “Given the success of the first human trial of NIAGEN^®, we have been eager to generate additional human data demonstrating NR is an effective precursor to NAD+.”

 

http://investors.chr...icle&ID=2147451

 

[Kevnzworld]

Re:
ChromaDex Initiates Second Human Clinical Study on NIAGEN® -The World’s First and Only Commercially Available Form of Nicotinamide Riboside (NR)

Maybe the results of the second test will actually get published.......the results from the first never were, which doesn't necessarily bode well.
That being said I still take 500 mg a day in divided doses

[Bryan_S]

Is this Tom?

 

http://www.nicotinamide-riboside.net

[midas]

Is this Tom?

 

http://www.nicotinamide-riboside.net

 

The first line....

 

"Nicotinamide riboside (NR), also known as vitamin B3"...............WTF!

[Ethic]

Is this Tom?

 

http://www.nicotinamide-riboside.net

 

 

What makes you think that?

 

Site is a total crap as it is, because it does not link to any sources.

[Bryan_S]

Just did a Whois the site belongs to Andre Thomas who we all know. Guys I see he needs some help, PM him and see what he needs rather than poke at him. Sometimes the French to English translations also need some adjustment. 

Edited by Bryan_S, 12 March 2016 - 07:41 PM.

[Tom Andre F. (ex shinobi)]

Yes nicotinamide-riboside.net is mine.. Sorry im still working on it. I didnt even put the source. Maybe tonight..

Im not sure how you did found it bryan lol It is definitely not finish

Btw, any help is welcome. The goal is to make a summary of all topic related to the molecule like i did for pterostilbene.com

Edited by Tom Andre F. (ex shinobi), 12 March 2016 - 08:20 PM.

[Tom Andre F. (ex shinobi)]

Ok, I just updated the blog with the related references, I hope I did nothing wrong. Will add some articles about it soon

[Bryan_S]

Im not sure how you did found it bryan lol It is definitely not finish

 

I can see it isn't completed. I would think you'd expect me to see these things as or soon after they are posted. It was the page format that tipped me off it might be you. I run a 24 hour and 1 week timeframe search thru the web crawlers every one or 2 days for this threads continuing content.

 

Good start 

[Ethic]

Unfortunately I have to revise a recommendation, highly dosed Tryptophan or its metabolites can cause Eosinophilia-myalgia syndrome (EMS) in some individuals.

 

Post-epidemic eosinophilia myalgia syndrome associated with L-Tryptophan

http://www.ncbi.nlm....les/PMC3848710/

 

 

Eosinophilia-myalgia syndrome induced by excessive L-tryptophan intake from cashew nuts

 

http://www.degruyter...d-2015-0014.pdf

 

 

It was good while it lasted...I'll stick to Niacin.

[Bryan_S]

Form 8-K for CHROMADEX CORP.

https://biz.yahoo.co...11/cdxc8-k.html

 

Item 1.01. Entry into a Material Definitive Agreement

On March 11, 2016, ChromaDex Corporation, a Delaware corporation (the "Company") entered into a Securities Purchase Agreement (the "Purchase Agreement") with an existing stockholder pursuant to which the Company agreed to sell in a registered direct offering a total of 384,615 units (the "Units") at a purchase price of $1.30 per Unit, with each Unit consisting of one share ('Shares") of the Company's common stock, par value of $0.001 per share ("Common Stock"), and one unregistered warrant (the "Warrants") to purchase one half of a share of Common Stock (the "Warrant Shares"). The Common Stock and the Warrants are immediately separable and will be issued separately. The Company has not engaged an underwriter or placement agent in connection with this offering.

 

I don't normally concentrate on the company business but here we can see they are in the process of raising half a million dollars to further some objective ahead.

[stefan_001]

Form 8-K for CHROMADEX CORP.
https://biz.yahoo.co...11/cdxc8-k.html

Item 1.01. Entry into a Material Definitive Agreement
On March 11, 2016, ChromaDex Corporation, a Delaware corporation (the "Company") entered into a Securities Purchase Agreement (the "Purchase Agreement") with an existing stockholder pursuant to which the Company agreed to sell in a registered direct offering a total of 384,615 units (the "Units") at a purchase price of $1.30 per Unit, with each Unit consisting of one share ('Shares") of the Company's common stock, par value of $0.001 per share ("Common Stock"), and one unregistered warrant (the "Warrants") to purchase one half of a share of Common Stock (the "Warrant Shares"). The Common Stock and the Warrants are immediately separable and will be issued separately. The Company has not engaged an underwriter or placement agent in connection with this offering.

I don't normally concentrate on the company business but here we can see they are in the process of raising half a million dollars to further some objective ahead.

If I recall well they stated that they may raise some cash as they plan to uplist exchange. Likely not related to research.

[Tom Andre F. (ex shinobi)]

 

Im not sure how you did found it bryan lol It is definitely not finish

 

I can see it isn't completed. I would think you'd expect me to see these things as or soon after they are posted. It was the page format that tipped me off it might be you. I run a 24 hour and 1 week timeframe search thru the web crawlers every one or 2 days for this threads continuing content.

 

Good start 

 

Thank you !

 

Well you seen it very fastly after i published it.. Impressive Bryan. Im working on it only within my free time and I think on the long run it would be interesting to have a summary from this thread into one blog with different title for each topic for instance the sublingual part, the mechanism etc.. On the forum its good to discuss and debate but very hard for some to use the googlesearch bar from this forum sometimes. And its unfortunate because the work / research done here is very good.

[Kirito]

Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice:

 

http://www.ncbi.nlm....pubmed/26970090

Edited by Kirito, 15 March 2016 - 02:57 AM.

[Ohm]

Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice:

 

http://www.ncbi.nlm....pubmed/26970090

 

Very interesting.

[Bryan_S]

Science News

Mitochondrial metabolism linked to acute kidney injury

 

PGC1 alpha works through NAD 'aging molecule' to guard against stress; research offers new therapeutic target for acute kidney injury

 

https://www.scienced...60316151353.htm

 

Here we go . . . I thought this was appropriate to mention. I think this NAD+ story keeps getting stronger and stronger.

 

 

"Within the kidney there is abundant ATP and mitochondria to carry out this essential chemical function." said Parikh. "We hypothesized that mitochondria might also be serving as an essential and early target for the various risk factors leading to kidney injury." 

 

"One of the most robust reactions was a local deficiency of niacinamide [an analog of vitamin B3] within the injured kidney," said Parikh. Vitamin B3 serves as a precursor for NAD, the 'aging molecule' at the heart of energy metabolism. "Recent studies have suggested that NAD levels fall with aging or with diabetes, which are two of the major risk factors for kidney injury. That was when a lightbulb went on."

To determine if increasing the level of NAD would accelerate recovery from kidney injury, the researchers administered extremely large quantities of vitamin B3 nutritional supplements -- the human equivalent of hundreds of tablets -to the genetically impaired mice.

"We found that, following administration of the supplements, the kidneys were able to resist both toxic and ischemic stress," said Parikh. "Normal doses of nutritional supplements are probably not going to cure kidney injury, but these experiments provide a proof-of-concept that metabolic manipulation of NAD might be a new avenue for the development of future therapies.

"Our work draws some new connections between PGC1 alpha and the NAD molecule, and their effects on metabolism," added Parikh. "If indeed NAD levels fall with aging, the kidney may be a new window to thinking about how we study aging overall and may take us outside the world of acute injury to more broadly consider how the body deals with environmental stressors throughout life."

Edited by Bryan_S, 29 March 2016 - 09:18 PM.

[Bryan_S]

 

Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice:

 

http://www.ncbi.nlm....pubmed/26970090

 

Very interesting.

 

 

http://onlinelibrary...acel.12461/full

 

I think this is the complete study.

[Logic]

 

 

Nicotinamide mononucleotide supplementation reverses vascular dysfunction and oxidative stress with aging in mice:

 

http://www.ncbi.nlm....pubmed/26970090

 

Very interesting.

 

 

http://onlinelibrary...acel.12461/full

 

I think this is the complete study.

 

 

Complete paper for any study FOC:

http://sci-hub.io/

 

Longecity should be sponsoring this..?

[Bryan_S]

A Study Investigating the Effects of Niagen™ in Healthy Adults. (15NRHC)

Posted March 17th

https://clinicaltria...how/NCT02712593

 

 Purpose

This study will evaluate the effect of repeated doses of Niagen™ on Niagen™ metabolite concentrations in blood, urine and muscle. There will be 3 doses of Niagen™ compared to a placebo. One quarter of subjects will receive the low dose of Niagen™, one quarter will receive the moderate dose of Niagen™, one quarter will receive the higher dose of Niagen™ and one quarter will reveive the placebo.

 

Dietary Supplement: Niagen™ 100

Dietary Supplement: Niagen™ 300

Dietary Supplement: Niagen™ 1000

Other: Placebo

 

 

Estimated Enrollment:140Study Start Date:March 2016Estimated Primary Completion Date:December 2016 (Final data collection date for primary outcome measure)

 

More studies, 6 as of this date.

https://clinicaltria...e&Search=Search

[stefan_001]

Frank Jaksch uses 500mg NR per day. Several Chromadex employees between 250 and 500mg / day. Some on ot for 3 years. Very interesting results conf call. Worth to listen

[HappyPaul]

Frank Jaksch uses 500mg NR per day. Several Chromadex employees between 250 and 500mg / day. Some on ot for 3 years. Very interesting results conf call. Worth to listen

 

Where can we listen?  Thank you

[docmaas]

 

Frank Jaksch uses 500mg NR per day. Several Chromadex employees between 250 and 500mg / day. Some on ot for 3 years. Very interesting results conf call. Worth to listen

 

Where can we listen?  Thank you

 

There is a transcript here:  http://seekingalpha....ipt?part=single

 

I had to register to read it.  It's an investment advice site but you can opt out of further communication by choosing judiciously.

 

Mike