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Ashitaba Liquid Chalcone Group Buy

ashitaba chalcone group buy dementia ngf cancer xanthoangelol

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#1 resveratrol_guy

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Posted 28 January 2016 - 11:31 PM


Hi folks, I'm just trying to guage interest in this substance with regards to a group buy. Here is a paper describing the major components expected to be in it.

 

Over the past few months, I've put a ton of effort into sourcing and testing on my own dime, and I'm satisfied so far with the results.  I have farm photos and LC/MS data which I can post. But none of this matters if not enough people are interested. In the worst case, I have a personal inventory in the fridge which I wouldn't mind selling for $190/liter. However, I think that if we got a sizable group buy going, the price could be much lower.

 

Right now, supply is zero, but my farmer assures me that at least 100 L should be available by April. Shipping from the farm takes about 3 weeks.

 

And no, I don't actually want to run the buy. It's nontrivial to import liquid containers, divide them up, repackage them with appropriate customs documentation, and send them back out. So anyone who wants to do this for a reasonable fee per liter is invited to speak up. Wholesale cost will be somewhere in the range of $80-120/liter including shipping to the group buy manager, depending on quantity and exchange rate fluctuations. Cost will go up from there depending on how much we pay the manager, plus destination shipping and customs duties, if applicable, to the end recipient.

 

The ashitaba is raised in the highlands of Indonesia. On the minus side, I have to translate discussions with the help of my local friend, although at this point I'm satisfied with the farm operation so it's pretty simple to order. But on the plus side, it appears to be an isolated region very distant from urban pollution.

 

Thus far, personally, I've consumed a liter and a half. A friend of mine consumed a liter as well. She said it kept her up at night and lowered her body temperature, which I haven't noticed myself although fasting tends to do the same thing. Both of us have noticed some degree of stomach discomfort, but I have no indications of actual illness as a result. To err on the side of caution, however, I would recommend taking probiotics in advance, as this stuff is not sterilized in any way, and should therefore be presumed to contain some microbes. I've seen some positive effects, but my regimen is so multifaceted that it's hard to pin down the cause. So this is as much a mass health experiment, as a group buy. (But what group buy isn't?)

 

For that matter, you should be aware of the xanthotoxin and psoralen content. Xanthotoxin apparently makes it easier to sunburn until your liver fully neutralizes it. I don't know much else about it, but Google helps. Personally, I just take my ashitaba after my last sun exposure of the day, or in the morning if I have plans to go out.

 

My dosing has been as high as 40 or 50 mL on one day, although 20 is probably average.

 

The taste is very "medicinal", so let's be upfront about that. You're not buying cherry cola here. It's kind of like having a glass of lemony kale juice, only more bitter.

 

I mailed a liter to a US lab and got back the results a couple weeks ago. Arsenic, cadmium, lead, mercury, bisphenol A, aflatoxin, and ethyl acetate are all below detection thresholds. (It's possible that the heavy metals were bound up with some component of the chalcone, I suppose, but in that case I would expect low bioavailability.) I selected those substances because heavy metals are in some soils, bisphenol A is in plastic packaging, aflatoxin is produced by a mould under conditions of poor crop management, and ethyl acetate is used by some low quality vendors to extract chalcone. I attached a screen shot of the results which should be visible if you're logged in. Additionally, they were able to identify xanthoangelol and 4-hydroxyderricin, the main compounds suspected of bioactivity in humans. There's not much to show in that case except for 2 big spikes.

 

Anyway, let me know what y'all think.Attached File  test.jpg   72.5KB   1 downloads


Edited by resveratrol_guy, 28 January 2016 - 11:43 PM.

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#2 Ark

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Posted 29 January 2016 - 11:14 AM

Sounds interesting!

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#3 playground

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Posted 31 January 2016 - 11:08 AM

yes, sounds interesting :-)

I don't have the time to focus on this at the moment

as i'm working on different projects that require my

full attention.  ... but i will continue to  check back to

see how things are coming along.

 

playground



#4 lumia

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Posted 13 March 2016 - 01:00 AM

resveratrol_guy, I read your stuff on ashitaba and it seems pretty interesting, and ashitaba supplements on the market usually can't supply this much xanthoangelol. Can you refresh my memory regarding the chalcone content of the stuff you're taking?

#5 resveratrol_guy

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Posted 13 March 2016 - 01:03 AM

resveratrol_guy, I read your stuff on ashitaba and it seems pretty interesting, and ashitaba supplements on the market usually can't supply this much xanthoangelol. Can you refresh my memory regarding the chalcone content of the stuff you're taking?

 

Unfortunately my lab test only confirmed the presence of xanthoangelol and 4-hydroxyderricin, not the concentration. So it's possible that the chalcone was surreptitiously diluted. Granted, I happen to have a few ashitaba plants in my garden, and the color of the sap looks the same, which suggests to me that it's just what would be expected in the actual plant. But there's a lot of money at stake here, so I'm not completely confident.
 



#6 ceridwen

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Posted 13 March 2016 - 01:39 AM

Sounds interesting now

#7 ceridwen

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Posted 13 March 2016 - 02:03 AM

Sounds interesting now

#8 LongLife

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Posted 03 April 2016 - 11:55 PM

RESVERATROL_GUY:

 

We are into April, what is the prognostics on production?

Where will this be shipped from?

Thanks.



#9 resveratrol_guy

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Posted 04 April 2016 - 02:00 AM

RESVERATROL_GUY:

 

We are into April, what is the prognostics on production?

Where will this be shipped from?

Thanks.

 

Apart from my personal inventory, I have nothing at the moment, mainly due to a lack of interest on this thread. I'd be willing to sell my own supply as specified above, but I don't have enough to service a typical group buy. It comes from Indonesia as explained above.

 

I had paused consumption for a few months on account of my jaw surgery and various follow ups to that, as I didn't want neuroregeneration to enhance pain sensation. Just yesterday I started drinking it again. The only positive thing I can say about the flavor is that it's more delicious than garlic juice!
 



#10 normalizing

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Posted 04 April 2016 - 07:39 AM

im still confused, what is wrong with the actual extract of this plant that there needs to be a specific chalcone isolate?



#11 resveratrol_guy

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Posted 04 April 2016 - 02:10 PM

im still confused, what is wrong with the actual extract of this plant that there needs to be a specific chalcone isolate?

 

Nothing. I've actually eaten dried stems, which also contain some chalcone. It's a question of economics. Why ship a ton of vegetable fiber that you can buy at the grocery store, in order to obtain a small amount of the liquid you actually want? You can buy powerdered ashitaba on Amazon if you want to go that route.
 



#12 normalizing

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Posted 04 April 2016 - 08:22 PM

on another note, maybe people arent so interested in this because there is not a single pronounced effect report from consuming this thing.



#13 resveratrol_guy

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Posted 05 April 2016 - 10:52 PM

on another note, maybe people arent so interested in this because there is not a single pronounced effect report from consuming this thing.

 

All the data comes from rat studies. The only human data we have is related to the consumption of the whole plant. It's assumed, but not proven, that the sap is responsible for most of the benefits. I'm not aware of anyone else drinking it.
 



#14 resveratrol_guy

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Posted 07 May 2016 - 05:11 AM

I realize that this thread is basically dead, but I figured I should post this for the record.

I accidentally overdosed on liquid chalcone about 10 hours ago. It was basically just the result of carelessness. Anyway I drank about 60 mL, which isn't all that much above my norm. However, after the fact, I noticed that a bunch of undissolved chalcone was sitting on the bottom of the container, which would suggest that the concentration was also higher than normal. It also happens that I had about 500 mg of pterostilbene today, which is as high as I've ever dosed. I'd like to say that this was all some grand experiment, but it was really just the result of sloppy dosing. I had some chocolate on top of that, but not nearly enough to make me feel ill.

So, for the record, I've spent most of the afternoon and evening feeling very nauseous. I've never experienced a complete disgust for food to the extent that even the thought of steamed veggies is repugnant. This is way beyond lack of hunger. One odd thing is that I had a runin with a particularly pollusive truck about an hour after drinking it. The memory of the pollution odor is as vivid as any odor memory has ever been for me, that I can recall. It's been hard to stop replaying in my head. One downside to playing memory enhancement games is that we cannot necessarily choose which memories will be enhanced!

My body is also feeling quite hot, like I'm in the midst of hardcore lypolysis, although for some reason I'm not sweating. It's not a fever; my thermometer currently reads 97.8F, whereas last time I had a fever it was over 99F. I'm actually slurping ice water as I write this because it's the only thing I can get down and I feel the need to cool off, even though it's 76F in the house, which would normally make me feel cold.

Hopefully this will serve to dissuade anyone from drinking too much chalcone too quickly, if this ever becomes a more widely used supplement. I've actually had to use mind-over-matter at several moments today in order to suppress memories of tastes or odors. Pretty much anything food-related makes me want to vomit. I feel quite ill, although this does not feel like an infection, based on the lack of actual stomach cramps or fever chills.

 


Edited by resveratrol_guy, 07 May 2016 - 05:15 AM.

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#15 resveratrol_guy

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Posted 07 May 2016 - 11:27 PM

For once, I actually agree with whoever labelled my post as dangerous and irresponsible. I should have measured more carefully. But fortunately, I didn't, so at least we have some qualitative data in this vein. I seem to have returned to normal this morning, although my hunger level is still weak. I haven't taken any chalcone today, as I probably need a break.


Edited by resveratrol_guy, 07 May 2016 - 11:27 PM.

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#16 resveratrol_guy

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Posted 12 May 2016 - 04:13 PM

Here is more photographic evidence of ashitaba destroying mutated age spots. With N=2, my confidence is low, but it would interesting to know if there's any other research on this.


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#17 lumia

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Posted 14 May 2016 - 11:33 PM

I wonder if some of your responses has to do with ashitaba chalcones' anti-diabetic effect? That part is well-reported, including an increase in adiponectin at a dosage far lower than what you have consumed.



#18 lumia

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Posted 15 May 2016 - 12:09 AM

I just found something... more interesting. Ashitaba chalcones may be MAOIs. (PMID 24265870)

 

BiomolTher (Seoul). 2013 May 30;21(3):234-40

Xanthoangelol and 4-Hydroxyderricin Are the Major Active Principles of the Inhibitory Activities against Monoamine Oxidases on Angelica keiskei K.
Kim JH1, Son YK, Kim GH, Hwang KH.

Abstract

Monoamine oxidase inhibitors (MAOI) have been widely used as antidepressants. Recently, there has been renewed interest in MAO inhibitors. The activity-guided fractionation of extracts from Angelica keiskei Koidzumi (A. keiskei K.) led to the isolation of two prenylated chalcones, xanthoangelol and 4-hydroxyderricin and a flavonoid, cynaroside. These three isolated compounds are the major active ingredients of A. keiskei K. to inhibit the MAOs and DBH activities. Xanthoangelol is a nonselective MAO inhibitor, and a potent dopamine β-hydroxylase (DBH) inhibitor. IC50 values of xanthoangelol to MAO-A and MAO-B were calculated to be 43.4 μM, and 43.9 μM. These values were very similar to iproniazid, which is a nonselective MAO inhibitor used as a drug against depression. The IC50 values of iproniazid were 37 μM, and 42.5 μM in our parallel examination. Moreover, IC50 value of xanthoangelol to DBH was calculated 0.52 μM. 4-Hydroxyderricin is a potent selective MAO-B inhibitor and also mildly inhibits DBH activity. The IC50 value of 4-hydroxyderricin to MAO-B was calculated to be 3.43 μM and this value was higher than that of deprenyl (0.046 μM) used as a positive control for selective MAO-B inhibitor in our test. Cynaroside is a most potent DBH inhibitor. The IC50 value of cynaroside to DBH was calculated at 0.0410 μM. Results of this study suggest that the two prenylated chalcones, xanthoangelol and 4-hydroxyderricin isolated from A. keiskei K., are expected for potent candidates for development of combined antidepressant drug. A. keiskei K. will be an excellent new bio-functional food material that has the combined antidepressant effect.


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#19 normalizing

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Posted 15 May 2016 - 02:38 PM

so how are those any important MAOIs in comparison to some of the psychoactives in ayhuasca or general synthetics? better than deprenyl at what dose and what administration? lots of MAOIs out there, lots of potent synthetics but also quite a bit of natural ones, how are these any more important, a vital question I ask.


Edited by normalizing, 15 May 2016 - 02:42 PM.


#20 lumia

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Posted 16 May 2016 - 12:57 AM

A very PubMed search comes up with these:

 

The IC50 for harmine towards MAOA is "in the range of 0.05-0.1 mg/kg" (PMID9257326). If I'm converting it correctly, it sounds like in the range of 0.24-0.47μM.

 

Genistein: MAOB IC50 is 6.81μM, while for MAOA it's "more potent than standard deprenyl." (PMID27118978)

 

Azure B: MAOA IC50 of 11nm, and MAOB IC50 of 70nm. (PMID 22197611)

 

Bacopaside I: "IC50 and Ki values of 17.08 ± 1.64 and 42.5 ± 3.53 µg/mL, respectively, for MAO-A enzyme."(PMID 24449518) I converted it to something like 17.44μM.



#21 normalizing

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Posted 16 May 2016 - 01:47 PM

lumia whats stopping you from trying it and reporting?



#22 resveratrol_guy

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Posted 18 May 2016 - 04:14 AM

A very PubMed search comes up with these:

 

The IC50 for harmine towards MAOA is "in the range of 0.05-0.1 mg/kg" (PMID9257326). If I'm converting it correctly, it sounds like in the range of 0.24-0.47μM.

 

Genistein: MAOB IC50 is 6.81μM, while for MAOA it's "more potent than standard deprenyl." (PMID27118978)

 

Azure B: MAOA IC50 of 11nm, and MAOB IC50 of 70nm. (PMID 22197611)

 

Bacopaside I: "IC50 and Ki values of 17.08 ± 1.64 and 42.5 ± 3.53 µg/mL, respectively, for MAO-A enzyme."(PMID 24449518) I converted it to something like 17.44μM.

 

First of all, as to the antidiabetic effect, all I can say is that a moderate sip of the stuff (20-40 mL) shuts down my appetite almost immediately, starting with the offensive "laundry detergent" flavor and ending with a sort of cold feeling. (Cleansing the palate with a sip of carrot juice is highly recommended.) From that point on, typically for hours, my hunger is in remission.

 

The MAOI angle is not something I was aware of. From a safety perspective, I suppose this means this means that we should avoid these foods in order to prevent hypertensive tyramine crisis. Now that you mention it, although I had relatively little chocolate the day of the "attack", I'd had 100 g of 86% dark the day before. It's possible that was close enough in time to the chalcone overdose that I did, in fact, have some such crisis.

 

Thanks for bringing this to our attention. It's an important safety consideration, if nothing else. For my part, I skipped a day or two after the attack, and have since resumed at 20-40 mL/d.


Edited by resveratrol_guy, 18 May 2016 - 04:16 AM.


#23 normalizing

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Posted 18 May 2016 - 05:14 PM

resveratrol guy why dont you just genetically test yourself and go from there. this is quite silly



#24 resveratrol_guy

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Posted 21 May 2016 - 04:49 AM

resveratrol guy why dont you just genetically test yourself and go from there. this is quite silly

 

Test for which SNP? I'm not sure why it matters at this point, although obviously it might have helped before the overdose. Anyway I have 23andMe.



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#25 lumia

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Posted 18 June 2016 - 06:34 PM

lumia whats stopping you from trying it and reporting?

 

Sorry for replying so late, but trying what?


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