11 replies to this topic

[Nate-2004]

I've been debating about which supplements to keep in my regimen. I had been taking pterostilbene/resveratrol assuming that it activates SIRT1/3. According to this study however, neither do any such thing. Basis includes pterostilbene in their NR formula due to this very claim that Sirtuins get activated by it. What's the deal?

 

I see a limited number of posts about pterostilbene. There's also very little information filled in on the Wikipedia page.  Does this actually work to activate Sirtuins or not? Should I bother with this supplement? I am taking honokiol for the same reason.

 

Abstract

Recent studies have implicated resveratrol and pterostilbene, a resveratrol derivative, in the protection against age-related diseases including Alzheimer's disease (AD). However, the mechanism for the favorable effects of resveratrol in the brain remains unclear and information about direct cross-comparisons between these analogs is rare. As such, the purpose of this study was to compare the effectiveness of diet-achievable supplementation of resveratrol to that of pterostilbene at improving functional deficits and AD pathology in the SAMP8 mouse, a model of accelerated aging that is increasingly being validated as a model of sporadic and age-related AD. Furthermore we sought to determine the mechanism of action responsible for functional improvements observed by studying cellular stress, inflammation, and pathology markers known to be altered in AD. Two months of pterostilbene diet but not resveratrol significantly improved radial arm water maze function in SAMP8 compared with control-fed animals. Neither resveratrol nor pterostilbene increased sirtuin 1 (SIRT1) expression or downstream markers of sirtuin 1 activation. Importantly, markers of cellular stress, inflammation, and AD pathology were positively modulated by pterostilbene but not resveratrol and were associated with upregulation of peroxisome proliferator-activated receptor (PPAR) alpha expression. Taken together our findings indicate that at equivalent and diet-achievable doses pterostilbene is a more potent modulator of cognition and cellular stress than resveratrol, likely driven by increased peroxisome proliferator-activated receptor alpha expression and increased lipophilicity due to substitution of hydroxy with methoxy group in pterostilbene.

 

 

Edited by Nate-2004, 27 June 2016 - 05:02 AM.

[APBT]

FULL TEXT

 

Attached Files

•  Low-dose pterostilbene, but not resveratrol, is a potent neuromodulator in aging and Alzheimer's disease.pdf   783.64KB   35 downloads

[Nate-2004]

FULL TEXT

 

Thanks. I'm not sure why they called it "low dose" as they used a dosage of 120mg/kg which is a whole lot higher than I'm taking now (100mg daily compared with what would be 9 grams daily).

 

The other benefits this study shows are pretty significant though. Its effects on inflammation and an increase in MnSOD in the brain means we should not dismiss the value of pterostilbene simply because it doesn't activate SIRT1 after all. I have a neurodegenerative disorder called essential tremor and perhaps I should continue taking pterostilbene for that reason alone, though this study's concern was alzheimer's, I'm sure it's not just a benefit for that particular disease.

 

I'll cut out resveratrol because according to this and other studies the bioavailability appears low and in addition, has no real effect on the above much less SIRT1. That cuts the price in half at least, Jarrow pterostilbene is $12 vs NOW foods pterostilbene/resveratrol $25.

[davis89x]

Thanks. I'm not sure why they called it "low dose" as they used a dosage of 120mg/kg which is a whole lot higher than I'm taking now (100mg daily compared with what would be 9 grams daily).

 

As I understand, You've missed the information or sth, that it was for mouses.

For human, for Your weight it would be 730mg.

[Nate-2004]

Not sure what I'm ill informed about. Why not just tell me what it is instead of marking it ill informed?

 

Anyway:

 

As I understand, You've missed the information or sth, that it was for mouses.

For human, for Your weight it would be 730mg.

 

120mg / kg was the dosage in the study. 120mg * 79kg (my weight) = 9,480mg. 

 

Where are you getting 730mg?

 

What is sth?

Edited by Nate-2004, 28 June 2016 - 09:40 PM.

[pamojja]

What is sth?

 

Guess 'something'.

 

See for example here:

 

converting-dosages-from-animal-studies/
 

[Nate-2004]

 

What is sth?

 

Guess 'something'.

 

See for example here:

 

converting-dosages-from-animal-studies/
 

 

 

Thanks so much. So ~700mg or so for the referenced study on pterastilbene.

 

So anyway, Basic includes pterastilbene in their NR product and I believe this is because they think it activates sirtuins. This study proves otherwise.

 

However because of this study I've been megadosing this week to see how that might effect my essential tremor problem. So far it's promising. I do notice much less of a tremor lately but I can never trust the initial results, I want to see what happens long term. It also contains resveratrol so I can't be sure it isn't that. I've also seen other placebo effects happen when testing new things. Last night I was putting together a desk which involved a lot of screw driving, that task is usually quite difficult for me since I can't guide the screwdriver into the slots without two hands helping to steady myself. Last night I only needed one hand and up until a certain point I had no problems guiding it in.

 

I'm still confused as to why they conclude that the levels of pterostilbene were "low doses" and "diet achievable" when they were using the human equivalent of 700+ mg. That has to be some kind of error or I'm misinterpreting the words "120mg/kg of diet" to mean 120mg/kg of pterostilbene. It's confusing what they mean.

 

Five-month-old male and female SAMP8 were fed with either resveratrol or pterostilbene at an identical dose (120 mg/kg of diet) for 8 weeks or control diet, 120 mg/kg of diet equated to the content of resveratrol of in 2 glasses of wine. Animals were kept on a 12-hour light and 12-hour dark cycle with free access to food and water. Pterostilbene dose was kept identical to that of resveratrol to determine potency differences

 

Edited by Nate-2004, 06 July 2016 - 03:53 PM.

[pamojja]

That has to be some kind of error or I'm misinterpreting the words "120mg/kg of diet" to mean 120mg/kg of pterostilbene. It's confusing what they mean.

Five-month-old male and female SAMP8 were fed with either resveratrol or pterostilbene at an identical dose (120 mg/kg of diet) for 8 weeks or control diet, 120 mg/kg of diet equated to the content of resveratrol of in 2 glasses of wine.

I read mg per kg of diet to mean exactly that.

 

 

https://en.wikipedia...aboratory_mouse

 

Food intake is approximately 15 g (0.53 oz) per 100 g (3.5 oz) of body weight per day; water intake is approximately 15 ml (0.53 imp fl oz; 0.51 US fl oz) per 100 g of body weight per day.^[5]

 

Guess a mice weights about 20 gram.

[Tom Andre F. (ex shinobi)]

Nate, there is plenty explanation that can be given to that, but we speak about a certain tissue and a certain condition. Still pterostilbene improved this condition as you noted.

 

Still I wrote an article that explain that pterostilene is a direct activator of sirt1 unlike resveratrol for instance and give some reference here:  http://www.pterostil...irt1-activator/

 

there is lot of study that show it does increase SIRT1 :

 

SIRT1 activation by pterostilbene attenuates the skeletal muscle oxidative stress injury and mitochondrial dysfunction induced by ischemia reperfusion injury

http://link.springer...0495-016-1258-x

 

Restoration of sirt1 function by pterostilbene attenuates hypoxia-reoxygenation injury in cardiomyocytes

http://www.sciencedi...014299916300954

 

 

 

[Nate-2004]

Nate, there is plenty explanation that can be given to that, but we speak about a certain tissue and a certain condition. Still pterostilbene improved this condition as you noted.

 

Still I wrote an article that explain that pterostilene is a direct activator of sirt1 unlike resveratrol for instance and give some reference here:  http://www.pterostil...irt1-activator/

 

there is lot of study that show it does increase SIRT1 :

 

SIRT1 activation by pterostilbene attenuates the skeletal muscle oxidative stress injury and mitochondrial dysfunction induced by ischemia reperfusion injury

http://link.springer...0495-016-1258-x

 

Restoration of sirt1 function by pterostilbene attenuates hypoxia-reoxygenation injury in cardiomyocytes

http://www.sciencedi...014299916300954

 

Interesting, so these are showing the opposite outcome. Maybe it was the low dose through diet in the study I cited in the original post that prevented SIRT1 from being activated. Though just to point out, these studies you linked are all in vitro on mouse cells it appears.

 

Are there any studies in humans showing SIRT1 is activated by pterostilbene? The evidence is quite inconclusive, Google search gets more support for evidence that it does not, but that's people's interpretation of the study I linked at the beginning.

[Tom Andre F. (ex shinobi)]

I really dont know or a deprivation of the cells by beta amyloid plaque or something else that also block the B3s precursor such as NR to activate sirt1 too (see the specific thread my last post).

 

http://link.springer...0495-016-1258-x this one is vivo usng pterostilbene

 

you have also some studies done in vivo by sinclair using resveratrol and since we know pterostilbene is better..

 

 

[Tom Andre F. (ex shinobi)]

To close this topic:

 

Nicotinamide restores cognition in AD transgenic mice via a mechanism involving sirtuin inhibition and selective reduction of Thr231-phosphotau

 

http://www.ncbi.nlm....les/PMC2617713/

 

Means what I thought was probably true: you have to not activate SIRT1 in AD mice brain. Thats probably why pterostilbene did not.