37 replies to this topic

[Logic]

Mitochondria are important in aging, and the process by which they become damaged and contribute to degenerative aging starts with the fact that they emit reactive oxidants as a byproduct of their normal operation...

 

...the most potent anti-oxidants were those that targeted the...mitochondria...the most potent mitochondrial targeted anti-oxidant was Tiron...which provided 100%, protection of the skin cell against UVA sun damage and the release of damaging enzymes causing stress-induced damage...

• Resveratrol...was found to protect against 22% of both the ultraviolet A radiation and stress-induced damage.
• NAC...offered 20% protection against oxidative stress and 8% against UVA,,,
• curcumin offered 16% protection against oxidative stress and 8% against UVA.
• In comparison Tiron offered 100% protection against UVA radiation and 100% protection against oxidative stress...

http://www.ncl.ac.uk...nger-for-longer

 

Tiron chelates released Iron in the mitochondria where the released iron would normally damage mtDNA.
Metals like iron and copper also catalyse the reactions that lead to Advanced Glycation Endproducts and Tiron looks to be effective, in vivo, here too.
http://www.ncbi.nlm....pubmed/17585685

 

It has undergone tox studies in pregnant rats with fetal size issues showing up at 3000 mg/kg/day, making the no observable adverse effect level (NOAEL) for maternal and developmental toxicity; 1500 mg of Tiron per kg per day, in rats.

That works out to a NOAEL of 486 mg/kg/day  in humans, or 3400 mg per day in a 70 kg human.

http://www.ncbi.nlm..../pubmed/1882131

NB That the above is NOT the recommended dosage, is the highest safe dosage

 

There are no studies I can find that give any indication of the optimal dosage in humans, so taking Nilotinib would be pretty experimental...

 

In-vivo studies:

 

Developmental toxicity evaluation of tiron (sodium 4,5-dihydroxybenzene-1,3-disulfonate) in mice.
http://www.ncbi.nlm..../pubmed/1882131

Prevention by Tiron (sodium 4,5-dihydroxybenzene-1,3-disulfonate) of vanadate-induced developmental toxicity in mice
http://onlinelibrary...480207/abstract

Protective effect of Tiron (4,5-dihydroxybenzene-1,3-disulfonic acid disodium salt) against beryllium-induced maternal and fetal toxicity in rats
https://www.research...oxicity_in_rats

Chelation therapy and vanadium: Effect on reproductive organs in rats
http://nopr.niscair....(6) 515-523.pdf

Cotherapy of Tiron and selenium against vanadium induced toxic effects in lactating rats
http://www.ncbi.nlm....les/PMC4575759/

Acute superoxide scavenging reduces sympathetic vasoconstrictor responsiveness in short-term exercise-trained rats
http://jap.physiolog...ent/114/11/1511

Cytoprotective potential of tiron and methyl palmitate against acetaminophen-induced acute liver injury
http://www.nrcresear.../cjpp-2015-0270

Prevention by Tiron (sodium 4,5-dihydroxybenzene-1,3-disulfonate) of vanadate-induced developmental toxicity in mice
http://onlinelibrary...480207/abstract

Superoxide Anions Contribute to Impaired Regulation of BloodPressure by Nitric Oxide During the Development ofCardiomyopathy
http://jpet.aspetjou...3/1643.full.pdf

Efficacy of Tiron for Enhancing the Excretion of Uranium from the Rat
http://het.sagepub.c.../3/195.abstract

Role of combined administration of Tiron and glutathione against aluminum-induced oxidative stress in rat brain.
http://sci-hub.bz/10...emb.2006.12.001
 

Tiron Group buy

 

I will be getting a 25 gram bottle of Tiron from the same suppliers as Nilotinib (3rd buy) for $ 87.50.
Adding it to the the Nilotinib Parcel will negate the $ 40 courier fee to the USA. 
USPS Priority Mail Small Flat Rate Box is $ 6.80.  International price calculator.

 

If anyone else wants Tiron, for research purposes, contact me.

I will need your address to work postage and PayPal fees and will give you my PayPal/Email address to make payment.

 

Edited by Mind, 03 April 2017 - 09:00 PM.

[Logic]

The following people have shown interest in Tiron:

• SuicidalTraveler
• richard hnry
• Qowpel

 

The following people have paid for Tiron:

• tiron     1x 25gram vial
• Logic    2x

As my contact is flying on business atm we have until around 8 am South Africa time to get orders in and paid up.
I require the # of vials you want ($ 87.50 per 25 gram vial) and your postal address via PM to do a costing that includes postage.

I will send payment details with the costing.

Edited by Logic, 23 August 2016 - 10:32 AM.

[ceridwen]

Can this be taken orally?

[tunt01]

Tiron seems interesting.  It would probably do quite a bit of good with seafood consumption to offset any metal contaminants.  I was thinking about upping my cilantro intake for similar properties.

[Logic]

Can this be taken orally?

 

Yes its taken orally Ceridwen, but seems to have quite low bioavailability.

Perhaps this can be fixed with lysosomes or Cyclodextrin or something.

[Logic]

Tiron seems interesting.  It would probably do quite a bit of good with seafood consumption to offset any metal contaminants.  I was thinking about upping my cilantro intake for similar properties.

 

In conclusion, in the present study, we have shown the high binding capacity of the pigment from black sesame seeds toward heavy metals, such as lead, cadmium, and mercury, that are commonly found in food. The highest activity is associated with neutral pH values simulating the intestinal environment compared to the acidic pH values peculiar of the gastric compartments. Even at low concentrations (0.05 mg/mL), the pigment proved able to remove the metals to a significant extent (>65% for cadmium and >90% for mercury and lead). At such doses, the pigment is able to remove the metals completely at the highest levels that have been reported in contaminated food. A good chelating ability against lead and to a lower extent cadmium is retained by BSP, even with the metal at concentrations up to 200μM. The binding activity is not lost following digestion as simulated using a model of the gastrointestinal transit, and on the basis of competition experiments, it should not interfere with the absorption of essential metals, a drawback exhibited by several dietary supplements, such as cereal fibers.In combination with our previous observations indicating the marked antioxidant properties of BSP, these results highlight the potential of this low-cost, easily accessible material from plant sources as an ingredient of functional food or as a food supplement.

http://www.longecity...ndpost&p=766829

 

BSP should be a filler in every supp IMHO.

 

 

There is also intriguing research showing additive and even synergistic effects from combining intra and extracellular chelators.
The intracellular chelator gets the unbound metal out of the cell and 'passes the ball' to the stronger extracellular chelator for excretion.

 

The combination of a weak chelator which has a better ability to penetrate ceils, with a stronger chelator that penetrates cells poorly but has a more efficient urinary excretion, may result in a synergistic effect through iron shuttling between the two compounds.
http://www.ncbi.nlm..../BCA-01-151.pdfhttps://books.google...ellular&f=false

 

What makes Tiron intriguing is its ability to not only get into cells, but also into the iron rich mitochondria within cells where any unbound iron causes serious damage.

(Tiron gives 100% protection against sunburn due to this  property..! Think 'no skin aging due to sun exposure...')

 

Naturally its important to know the half life of your chelators for efficient chelation therapy...  That and the clinically effective dosage is proving very difficult to ascertain!

 

 

It would seem that the Mitochondria damaged by unbound iron may also overload the lysosome with iron when recycled.
The iron causes a lysosome damaging 'fizz' when the acid within them reacts with it.
I am sure you are aware of the importance of a properly functioning garbage recycling 'production line' within cells...

Hence my getting and wanting to 'test' Tiron.

Edited by Logic, 24 August 2016 - 11:12 PM.

[Dorian Grey]

IP6 (Inositol Hexaphosphate)...  Cheaper, easier, better!

 

http://www.ncbi.nlm....les/PMC2703150/

[Logic]

IP6 (Inositol Hexaphosphate)...  Cheaper, easier, better!

 

http://www.ncbi.nlm....les/PMC2703150/

 

 

Ye its good stuff!  Except for probably downregulating autophagy like IP3:

http://www.longecity...ndpost&p=763863

 

I take Inositol in the morning due to autophagy being upregulated at night/fasted and it having a half life of around 5.5 hrs in babies. Probably longer in adults.

[ekaitz]

Is this the same compound as the one mentioned on this article, called "mitoiron"?

 

http://www.scienceda...60720105042.htm

[SuicidalTraveler]

Anything new?

[Logic]

tiron (his longecity nic) has received his tracking # as have I.

tiron should receive his Tiron on monday, I will have to wait a little longer.

 

The Tiron was tested by the lab and found to be as advertised.

 

If anyone else is interested, contact me.

[DareDevil]

Hi Logic I would be interested. What intake is recommended? SubQ shots in suspended solution or taken orally with something else?

 

DareDeveil

[Logic]

It would seem that there is enough interest in T to get some more again.
Plz post here (amount) if interested.

I will also require your postal address and preferably Email address (for alternate comms)

 

As T has very little research on its effects etc; it's important to post results here too.

[ekaitz]

Im interested, but have no idea what dosage/protocol is adviced with this substance..

[Logic]

My research into dosage with Tiron did not turn up much useful information and was quite extensive.
I dont have the time I would like to look further.
This is a good start:

https://www.google.c...ron oral dosage

 

I posted a lot of info here:
http://www.longecity...ed-antioxidant/

including research on the concurrent use of intra and extra cellular chelators...

Tiron is certainly very experimental.
 

Wololo Has placed a firm order for 50 grams.
anyone else?
I need to buy more as the SA Post Office has misplaced my package.  Via courier this time.
The order will be placed on the 27th due to the Chinese new year.
 

[Valijon]

Hi Logic,

Anything more on how to increase bioavailability of Tiron? Also, I don't think you published a total price per gram. Any idea how much would be a good dose?

[DareDevil]

Hi Logic,

 

Thanks for counting me in for 1 x 25g vial of Tiron.

 

At dosage of 300mg/day a vial lasts 83 days.

This allows for a 1 month trial with up to triple dosage.

I will be testing it for effects, and modulate the dose.

 

Thanks for the Group Buy

 

DareDevil

[Leni]

Are there still group buys to buy tiron? Is there any more known about what dosage could be effective but (hopefully) harmless. Is anyone using tiron as potential topical/oral supplement to try to counteract photoaging? 

Edited by Lenie, 03 February 2017 - 11:53 AM.

[DareDevil]

Hi Lenie,

 

You'll want to contact Logic about this as one might be forthcoming.

 

Cheers,

DareDevil

[Adamzski]

Has anyone now taken this for a while?

[Iuvenale]

If there is another group buy, I would like to be included.

[Leni]

If there is another group buy, I would like to be included.

So would I. I'm surprised that there has not been much more enthusiasm about Tiron. I have not been able to find any anecdotal info of someone taking Tiron here.

[ceridwen]

Yes I think I would be interested in this. Thanks

[DareDevil]

BUMP

 

It is supposed to be good stuff !

 

[Leni]

Yes, really: who has been using it? What dosage? Any anecdotal info to share (also with regard to UVA/UVB protection)?

 

And are there still any group orders taking place?

 

Tiron sounds too good to be true (so perhaps it is too good to be true): again, why is it not much  much more popular on Longecity?

[akshay ukey]

Hello guys. Is anyone using this.I live in India and I can get Tiron at very cheap rates. But can anyone suggest proper guidelines to consume it. I see 300mg/day being mentioned on this thread.

Edited by akshay ukey, 30 July 2017 - 05:06 PM.

[Qowpel]

I'm . Highly interested in buying and have a few hundred to spare at the moment. So can we make another group buy? Are there any chance of Tiron causing negative side effects especially when it comes to autophagy?

[meatsauce]

I can do a group buy in the US. I have a reliable supplier that I have used before. 

I can do a group buy in the US. I have a reliable supplier that I have used before. 

[tintinet]

Why not? Bitcoin profits up daily!

[akshay ukey]

Noob question: Will this prevent us from tanning?