72 replies to this topic

[PAMPAGUY]

Don't blame you for trying to save money. What you have found is a legit lab supplier for labs that do work on animals. The rapa is cheap, but going to guess that you have to be a licensed lab for them to sell to you. Also, do you want to be a compounding pharmacy in your home just to save a few hundred dollars a year. Rapa is not soluble in water, so thats another problem to overcome. Just always remember that rapa is not a supplement, it is a serious drug. For your own safety. Mouth sores are the first sign that your immune system is being compromised. Good Luck in your experiment.

[smithx]

Also, if you are taking 1mg to 4mg it is VERY hard to measure those quantities accurately without scales that cost thousands of dollars.

 

And taking double could be bad (8mg instead of 4mg).

 

Also, rapamycin even intermittent could be bad for us, so please note I am not recommending it to anyone.

[TaiChiKid]

In the rapa thread:  Is anyone taking this? Daredevil and others discuss dissolving a larger, measurable quantity of rapa in 151 or higher alcohol, such as overproof rum.  You then take a small quantity (say a teaspoon) once a week or two, depending on how you arrange your othe regimen components.

 

For example, you might dissolve 100mg of rapa in 20 teaspoons of rum, thus making 20 x 5mg doses.

 

Then a cheap 1mg digital scale from FleaBay (or even a jewelers scale) would be fine.

Edited by TaiChiKid, 08 November 2017 - 07:02 PM.

[PAMPAGUY]

Like I said before, you are setting yourself up to be compounding pharmacy. Any mistake could compromise immune system. If Rapa OD, it is poison.

[TaiChiKid]

Agreed, Rampaguy.  Doing the protocol as DD suggested, you mix 100mg rapa with alcohol, in which it dissolves completely.  The 1mg schale gives an accuracy of +- 1mg.  You are then dividing either 99mg to 101mg of rapa into teaspoons, giving you 5mg +- 0.05mg.  Only do this if you are competent at doing first year university lab work.

[RWhigham]

Dissolving 500 mg of rapamycin powder in Everclear

• Get some 95 proof Everclear brand grain alcohol (ethanol) from the liquor store
• Get a 2 oz dropper bottle (organic grocery stores often carry them)
• Calibrate the dropper with ice cold ethanol from the freezer and calculate how much ice cold ethanol will give 1000 drops.
• Result: 5 mL of ice cold Everclear gave 250 drops from my dropper. Check 5 mL/250 = 0.02 mL = 20 mg = typical dropper drop size
• So 20 mL will give 1000 drops from my dropper bottle
• Put 20 mL of Everclear in the dropper bottle and add 500 mg of rapamycin powder. It will completely dissolve.
• Keep in the freezer. It will not freeze. It will keep better. It will take 100 weeks (almost 2 years) to use it up.
• Now you have 0.5 mg/drop. Add 10 drops to 1/4 cup of water for a 5 mg dose.

Edited by RWhigham, 11 November 2017 - 02:20 AM.

[tintinet]

Don't blame you for trying to save money. What you have found is a legit lab supplier for labs that do work on animals. The rapa is cheap, but going to guess that you have to be a licensed lab for them to sell to you. Also, do you want to be a compounding pharmacy in your home just to save a few hundred dollars a year. Rapa is not soluble in water, so thats another problem to overcome. Just always remember that rapa is not a supplement, it is a serious drug. For your own safety. Mouth sores are the first sign that your immune system is being compromised. Good Luck in your experiment.

 

 

WRT to mouth sores- AFAIK from the research I've read about this phenomenon, it's not necessarily a sign of immune system compromise, but appears to be an idiosyncratic phenomenon of uncertain mechanistic origin. Perhaps this is old data?

[PAMPAGUY]

I visited Dr. Green in May of this year for a full exam, and 2 hour conversation on using Rapa for anti-ageing. He is a Pathologist who has taken Rapa for 18 months. He also is in direct contact with other MD's and scientist in the field. He instructs and uses mouth sores as the first sign of an overdose on his patients and himself.
When it comes to my health and safety, I prefer to take the advice of trained professionals.

https://rapamycintherapy.com

[tintinet]

I visited Dr. Green in May of this year for a full exam, and 2 hour conversation on using Rapa for anti-ageing. He is a Pathologist who has taken Rapa for 18 months. He also is in direct contact with other MD's and scientist in the field. He instructs and uses mouth sores as the first sign of an overdose on his patients and himself.
When it comes to my health and safety, I prefer to take the advice of trained professionals.

https://rapamycintherapy.com

 

 

Whateva works for ya, cool. I'm not appealing to authority, just reflecting the science on the issue. I've not had mouth sores, so I can't comment on the experience. 

[VP.]

http://www.aging-us....cle/101319/text

Towards natural mimetics of metformin and rapamycin

Aging is now at the forefront of major challenges faced globally, creating an immediate need for safe, widescale interventions to reduce the burden of chronic disease and extend human healthspan. Metformin and rapamycin are two FDA-approved mTOR inhibitors proposed for this purpose, exhibiting significant anti-cancer and anti-aging properties beyond their current clinical applications. However, each faces issues with approval for off-label, prophylactic use due to adverse effects. Here, we initiate an effort to identify nutraceuticals—safer, naturally-occurring compounds—that mimic the anti-aging effects of metformin and rapamycin without adverse effects. We applied several bioinformatic approaches and deep learning methods to the Library of Integrated Network-based Cellular Signatures (LINCS) dataset to map the gene- and pathway-level signatures of metformin and rapamycin and screen for matches among over 800 natural compounds. We then predicted the safety of each compound with an ensemble of deep neural network classifiers. The analysis revealed many novel candidate metformin and rapamycin mimetics, including allantoin and ginsenoside (metformin), epigallocatechin gallate and isoliquiritigenin (rapamycin), and withaferin A (both). Four relatively unexplored compounds also scored well with rapamycin. This work revealed promising candidates for future experimental validation while demonstrating the applications of powerful screening methods for this and similar endeavors.

[VP.]

The lifespans of flies and worms are prolonged by limiting the activity of an enzyme common to all animals, finds a UCL-led study.

"We now think that Pol III promotes growth and accelerates ageing in response to a signal inhibited by rapamycin, and that inhibiting Pol III is sufficient to result in flies living longer as if they were given rapamycin. If we can investigate this mechanism further and across a wider range of species, we can develop targeted antiaging therapies."

https://www.scienced...71129131437.htm

[StanG]

Ozone8

 

The first time I took it I mixed it in rum and after I drank it, I saw just a tad and touched my finger to it and put it on my tongue - DUMB DUMB DUMB!!! It burnt for a couple of hours. Since then I have been taking about 6mg it once a week with rum after I have mixed it up and have had no problem. I can't say whether it helps but my research on RAPA indicates it makes sense to me.

[Ozone8]

Stan,

 

Your first experience mirrors what probably happened to me. I put it in a capsule with magnesium but some residue got on a glove with which I then handled the capsule. Popping it in my mouth and swallowing with water likely caused the reaction I described, although for some reason it didn't bother me until a couple of hours later. Since then I've been careful to keep the outside of the capsule RAPA free.

[roberth2309]

Taking 7m per week of rapamycin, 1000mg of Metformin per day, rifampicin 300mg per day, allantoin 200mg per day and NMN 150mg per day. No serious side effects after 6 weeks. Will get blood test in about 2 weeks

[PAMPAGUY]

I have been taking rapa for 1 year, started with 6 mg weekly and now take 8mg weekly.  Men have stronger TOR signal than women.

You might want to take a look at the following Longecity Forum concerning NAD+ supplementation.  As a former user of NR, I'm sold and

have been getting good results.  In my opinion 125 mg of NAD is insufficient.  There is a reason Dr. Sinclair takes 500 mg , but the cost is prohibitive.  Everything else your taking is excellent.  The Trifecta of anti-ageing are:

 

1.  TOR inhibition

2.  Glucose control

3.  NAD+ replenishment (Mitophagy)

[Ozone8]

PAMPAGUY,

 

Thanks for the report.

 

Would you mind providing your weight? 

 

Also, for what reason did you increase your weekly dose from 6 to 8 mg? Doesn't Dr. Green suggest a maximum of 6 mg/week? Have you had different results with the increased dosage?

[roberth2309]

One thing I have noticed is that any side effects seem to occur during the last day or two of the cycle. Also they start easing within a few hours of taking the new 7mg dosage.

[Ozone8]

What side effects are you experiencing?

[PAMPAGUY]

PAMPAGUY,

Thanks for the report.

Would you mind providing your weight?

Also, for what reason did you increase your weekly dose from 6 to 8 mg? Doesn't Dr. Green suggest a maximum of 6 mg/week? Have you had different results with the increased dosage?

[PAMPAGUY]

Im still in contact with Dr. Green. He personally has upped his dosage to 8mg weekly and so have I. Weight 178lbs. Contnue to lose. Goal 165 lbs. 71" tall
Reasons for raising dosage. Mens TOR signal is much stronger than womens. Many Murine studies show females getting a larger life span boost than males. Usually same dosage both sexes. I continue to take all necessary medication to follow the Trifecta of ageing.
TOR inhibition
Glucose control
NAD+ supplementation

[Ozone8]

Thanks for the further explanation. Evidently Dr. Green has not yet updated his website to reflect his current thinking.

 

At the same height (71") as you but weighing 145 lbs. I'm not looking to lose any but might elevate my dose from 5-6 to 7-8 mg per week. 

[PAMPAGUY]

Keep tight control on mouth sores and cut back if necessary. They are first sign of compromised immune system. Its a delicate balance and everyone is different.

[roberth2309]

The only effect is the occasional mild canker sore. Though I have noticed they are brcoming less common.

[StanG]

A couple of thoughts. Women weigh more than men which may account for the difference regarding dosage. Second; Eat a piece of apple or grapes or crackers right after you take the RAPA and see if you get the sores. I've never gotten the sores after the first time ref my Dec 1st post.  

[PAMPAGUY]

I have taken rapa 48 times and only 1 sore. Could have been a flu virus. They believe men's testosterone is driving force behind a stronger TOR signal. All that male agression could possibly be blamed on TOR.

[roberth2309]

Mine could have been driven by other factors. Haven't had for last two cycles. Prior to that I trashed my immune system taking dasatinib and quercetin.
Since I am supplementing with testosterone cream and testosterone is quite high now, I probably need to increase my rapa dose to 8mg also.

[Ozone8]

roberth2309,

 

You mention taking dasatinib/quercetin that "trashed" you immune system. What was your overall impression of that experience? Any benefits? What dosage did you take and for how long?

[roberth2309]

100mg of dasatinib, 3000mg quercetin & fisetin. Felt like I had a viral infection for 3 days. Felt weak and trashed... sore throat, low grade nausea.

[smithx]

I got a sore on at the very back of the left side of my jaw, behind my wisdom teeth, so I skipped a dose (I had been taking 4mg of rapamycin once every 10 days).

I then took another dose a few days ago, for the first time in 20 days and my sore has returned. It may have not gone away completely.

This is not the only time I've gotten a sore in my mouth or even in that location, so it may very well be a coincidence. And the rapamycin seems to make me feel much more energetic.

But I am concerned about immune suppression. Does anyone have any insights about this topic and how to determine if a mouth sore is resulting from the rapamycin or not?

[to age or not to age]

I was taking 5mg of rapamycin once a week, then cut back to every 10 days after 8 o r 9 months - total duration almost 11 months. I got a few mouth sores, my wife, who was also taking it, had frequent mouth sores and stopped around 3 months ago. 4 weeks ago I took a 5mg dose.  The next day I was out in busy close company.  I came down with some kind of flu the following day, and felt sick for 2 weeks.  I have discontinued rapamycin for now.  I have read that rapamycin may need not only to be taken intermittently, but for only a certain length of time.  In the case of the middle aged mice, they were dosed for only 3 months I believe, and yet gained in health and l lifespan. Also,

if the half life of rapamycin is 60 hours, over an extended period there will be a slight build up of itinq the body, a very small and slow build up, but still some. I also want to add that we also take resveratrol, curcumin (not within a day or two of taking rapamycin) and NR, so it is not clear whether any of these other compounds also knock down mtor, and whether there is an net increase in effect.